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Having amplified the short tandem repeat sequences within your DNA sample, you will then separate
out the fragments using gel electrophoresis (see Practical 6.1). Comparing the position of the bands
on the gels to a standard or reference you will be able to draw conclusions about the DNA sample
you started with. Requirements per student or group of students Notes Respirometer See the
diagram on the Student sheet. How did you know how to split your research between the rationale
and discussion. This resource hasn't been reviewed yet To ensure quality for our reviews, only
customers who have purchased this resource can review it Report this resource to let us know if it
violates our terms and conditions. And I did well on it apart from the method section, I am very
confused about my method, do I just outline a treatment of colic and use a study to show that vets
use it and show that it is effective. Supports independent learning through a range of additional
resources provided on the ActiveBook CD-ROM, included FREE in the back of each book.
Atmospheric pressure pushes the liquid along the tube, until the pressure in and outside the tube is
equal. Light readings can vary widely with time of day and cloud cover. U-tube respirometers would
be even better, if there is a class set available. My As coursework topic is colic in horses which is an
illness. Safety Wash your hands thoroughly after handling the snail. Not consenting or withdrawing
consent, may adversely affect certain features and functions. Edexcel Biology Core Practicals A2
core practicals: 1-looking for pattern (ecological studies) 2-Effect of temperature o. A key to
organisms likely to be found in the area The Field Studies Council produces a range of excellent
guides. The volume of air the person inhales and exhales can be calculated from the distance the lid
moves. If they might attempt to lift a piece of tape from an incubated dish, the agar plates should be
sealed around the circumference after incubation but before being returned for observation. Life and
Health science (step up science equivalent) Drop Psychology or English Literature. For example,
allergy to penicillin is not uncommon. How did you know how to split your research between the
rationale and discussion. In this activity you will learn how a spirometer works and how to interpret
the spirometer trace that is produced. Changing Skies 22 Hi there as I posted my AS Biology
coursework due to a lot of interest, I thought I'd post my A2 coursework to help a bit more. Reviews
Select overall rating (no rating) Your rating is required to reflect your happiness. Watch 9 years ago
A2 Edexcel Biology coursework example. Notes on the procedure Rate of respiration is not a
learning outcome in the specification. Write a review Update existing review Submit review Cancel
It's good to leave some feedback. My As coursework topic is colic in horses which is an illness. Label
the Petri dish on the base at the edge with your name, the date and the type of bacterium it is
inoculated with. 3 Flame the forceps and then use them to pick up an antibiotic disc or Mast ring. A
quadrat Either square or point quadrats can be used. Estimating animal populations Quadrats cannot
be used for mobile animals as these don’t stay in the quadrats. Procedure You may have the
opportunity to complete experimental work using restriction enzymes and gel electrophoresis or you
may use the simulation of this.
Methods of measuring abundance Density Count the number of individuals in several quadrats and
take the mean to give number per unit area, for example per metre squared (m22). A link to Google
Classroom that contains ALL of the following: 35. If you are using thick-walled capillary tubing it is
worth reminding students of the formula for working out the volume of oxygen used, and explaining
it again. This resource hasn't been reviewed yet To ensure quality for our reviews, only customers
who have purchased this resource can review it Report this resource to let us know if it violates our
terms and conditions. This resource hasn't been reviewed yet To ensure quality for our reviews, only
customers who have purchased this resource can review it Report this resource to let us know if it
violates our terms and conditions. I feel like I'm not detailed enough even though I've written quite a
lot already. Snails should be kept in moist conditions before the lesson, to encourage activity. Eye
protection is not necessarily required during microbial work, though it is good standard practice.
CLEAPSS guidance is in section 14.5.1 of the Laboratory handbook. However, they cannot be used
for measuring oxygen consumption. For example, you can see if there is a significant positive or
negative correlation between the factor you think is responsible for the pattern and the distribution of
the organisms you have recorded. No obvious. Read more about the condition Very Good: A book
that does not look new and has been read but is in excellent condition. Brine shrimps will breed and
produce cysts that can be collected. If resistance to breathing suddenly increases it may be due to
valves in the spirometer sticking. Large beaker or tank of salt water 1 substrate and algae, set up well
in advance. Restriction enzymes The use of restriction enzymes to cut DNA and electrophoresis to
separate the resulting fragments is possible using equipment available from NCBE (National Centre
for Biotechnology Education) and Bio-Rad. Movements of the chamber are recorded using either a
kymograph (pen writing on a rotating drum), a chart recorder, computer or datalogger. It is set up so
that adding air to the chamber makes the lid of the chamber rise in the water, and removing air makes
it fall. Having amplified the short tandem repeat sequences within your DNA sample, you will then
separate out the fragments using gel electrophoresis (see Practical 6.1). Comparing the position of
the bands on the gels to a standard or reference you will be able to draw conclusions about the DNA
sample you started with. Hide replies Empty reply does not make any sense for the end user Submit
reply Cancel zoebailey369 7 years ago report 5 very useful - thank you Show replies russellsuter 7
years ago Glad to be of assistance - thank you for the feedback Hide replies Empty reply does not
make any sense for the end user Submit reply Cancel JMScobie 9 years ago report 4 Extremely
useful. A link to Google Classroom that contains ALL of the following: 35. If conditions change
across a habitat, for example across a rocky shore or in a sloping meadow that becomes more boggy
towards one side, then systematic sampling along a transect allows the changes to be studied. See
other similar resources ?1.00 (no rating) 0 reviews BUY NOW Save for later Not quite what you
were looking for. How did you know how to split your research between the rationale and
discussion. If resistance to breathing suddenly increases it may be due to valves in the spirometer
sticking. Thanks 0 Report Reply Reply 16 8 years ago lucylu.1998 hi, i was wondering if you could
help me. Take care that the stimulus causes no harm to the snails. Community ? Groups Quotes Ask
the Author People Sign in Join Jump to ratings and reviews Want to read Buy on Amazon Rate this
book Edexcel Biology for A2 C.J. Clegg 4.75 4 ratings 1 review Want to read Buy on Amazon Rate
this book Edexcel Biology for A2 is a full colour textbook with supporting Dynamic Learning
website, written specifically for the new Edexcel specification. Accessibility, User Agreement,
Privacy, Payments Terms of Use, Cookies, CA Privacy Notice, Your Privacy Choices and AdChoice.
English Literature A-Level is edexcel alevel hard.
Raise the lid of the Petri dish and place the Mast ring firmly in the centre of the agar; if individual
discs are used they will need to be spaced evenly around the dish. 4 Tape the dish securely with two
pieces of adhesive tape (but do not seal it completely), then keep it upside down at room temperature
for 48 hours. Or have you been clambering over a rocky shore and spotted that the seaweeds grow in
distinctive bands and that you only find mussels where the tide is far out. Price from Blades
Biological, ?48.26 (2009) including post and packing, brine shrimp eggs and innoculum.
Requirements per student or group of students Notes Giant African land snail (or large garden snail)
Giant African land snails can be purchased in some pet shops or from Biological suppliers such as
Blades Biological. Discuss the limitations of your results and conclusions based upon them, and
suggest modifications that you could make to the procedure. If clover was recorded in 10 of the 25
squares that make up a 0.25?m2 quadrat frame, the percentage frequency would be 40%. Use aseptic
techniques when transferring the bacteria to the Petri dishes. A couple in my class had a similar
count, some others had theirs at about 5 I think you should be fine Posted from TSR Mobile 1
Report Reply Reply 4 9 years ago Henil Hey. I'm sure you are if you can't think of anything else,
you're probably done. A key to organisms likely to be found in the area The Field Studies Council
produces a range of excellent guides. The subject will feel some resistance to breathing when using a
spirometer. See also section 15.10 on respirometers in the CLEAPS Laboratory Handbook for details
of other (bulk) suppliers. 5?g of an actively respiring organism Use actively germinating peas, beans
or other seeds, or maggots, or woodlice. Because of this they should not use the spirometer while
exercising. I feel like I'm not detailed enough even though I've written quite a lot already. Reviews
Select overall rating (no rating) Your rating is required to reflect your happiness. See other similar
resources ?18.00 (no rating) 0 reviews BUY NOW Save for later Not quite what you were looking
for. If conditions change across a habitat, for example across a rocky shore or in a sloping meadow
that becomes more boggy towards one side, then systematic sampling along a transect allows the
changes to be studied. If your calculated rs value is less than the critical value, then the null
hypothesis is accepted. 10 Write a statistical conclusion for your experimental data. They may also
consider individual variation and sample sizes. A canister containing soda lime is inserted between
the mouthpiece and the floating chamber. Random sampling Frequently, ecologists notice a distinct
pattern that may be related to one or more factors at two sites. This practical requires a
microcentrifuge and the protocol is not downloadable from the NCBE website. For detailed guidance
on the care and breeding of brine shrimps see the publication Brine Shrimp Ecology by Michael
Dockery and Stephen Tomkins, available from: Homerton Brine Shrimp Project, Dept of Biological
Sciences, Homerton College, Cambridge CB2 2PH Tel: 01223 507175. Community Reviews 4.75 4
ratings 1 review 5 stars 3 (75%) 4 stars 1 (25%) 3 stars 0 (0%) 2 stars 0 (0%) 1 star 0 (0%) Search
review text Filters Displaying 1 of 1 review Menna 8 reviews 1 follower August 1, 2012 Detailed in
all topics But poor in ecology Like Comment Displaying 1 of 1 review Join the discussion Add a
quote Start a discussion Ask a question Can't find what you're looking for. You might estimate the
number of dandelions in each meadow by counting the number in several small areas and then
multiplying up to calculate a value for each meadow. If you have suitable data, you can calculate
correlation coefficients between your biotic and abiotic data. Topic 5: On the wild side 4.5.1 A
particular species can be ide. Notes on the procedure The aim of the practical is to show students a
method of determining bacterial sensitivity to different antibiotics and to give them practice at using
aseptic techniques. Accessibility, User Agreement, Privacy, Payments Terms of Use, Cookies, CA
Privacy Notice, Your Privacy Choices and AdChoice. Safety Wash your hands thoroughly after
handling the snail.
Small beaker of salt water For counting larvae on the second day. Allow the snail to get used to its
new surroundings for a few minutes until it has fully emerged from its shell. 2 Dampen a cotton
wool bud with water. 3 Firmly touch the snail between the eye stalks with the dampened cotton wool
bud and immediately start the stopwatch. Residual air prevents the walls of the bronchioles and
alveoli from sticking together. Take care that the stimulus causes no harm to the snails. Seller shipped
immediately but the book took over half a month to arrive, due to the carrier used on the first leg, to
get the package to USPS. Quadrats are laid down at regular intervals along the tape and the
abundance of species within each quadrat is recorded. See other similar resources ?1.00 (no rating) 0
reviews BUY NOW Save for later Not quite what you were looking for. Measure the length of time
between the touch and the snail being fully emerged from its shell once again, with its eye stalks
fully extended. 4 Repeat the procedure in step 3 for a total of 10 touches, timing how long the snail
takes to re-emerge each time. 5 Record your results in a suitable table. 6 Present your results in an
appropriate graph. The chapters follow the order suggested by the concept approach to the course.
Notes on the procedure Students need to carry out a study on the ecology of a habitat to produce
valid and reliable data (including the use of quadrats and transects to assess the abundance and
distribution of organisms and the measurement of abiotic factors). In this practical you will be using
a very simple respirometer, while considering the advantages of some of the slightly more complex
ones. The need for valid, reliable results should be considered and the random nature of error could
be investigated. Each centre holds equipment that can be used to conduct biotechnology practicals
for 25 students at a time. Which exam board dominate most of your exams? What exam board do
you hate the most. Community Reviews 4.75 4 ratings 1 review 5 stars 3 (75%) 4 stars 1 (25%) 3
stars 0 (0%) 2 stars 0 (0%) 1 star 0 (0%) Search review text Filters Displaying 1 of 1 review Menna 8
reviews 1 follower August 1, 2012 Detailed in all topics But poor in ecology Like Comment
Displaying 1 of 1 review Join the discussion Add a quote Start a discussion Ask a question Can't find
what you're looking for. Spirometers allow us to study both breathing and respiration. But working
with one’s own DNA can be uniquely motivating. The volume of air the person inhales and exhales
can be calculated from the distance the lid moves. The completion of Spearman’s rank correlation
provides an extension to this activity. I had a plan written by my teacher Posted from TSR Mobile
your work is really good. If PCR is to be used a full risk assessment should be obtained for the
procedure and carefully followed by both staff and students. About 2?cm3 of coloured liquid e.g.
water and food colour or equivalent. There are several publications available about the care of land
snails. They have all the equipment necessary for completing DNA fingerprinting, bacterial
transformation (genetically altering bacteria with a bioluminescent jellyfish gene), purification of a
useful protein, and the polymerase chain reaction (PCR). Suggestions for the presentation of
variation in graphs may also vary but need to identify anomalous results clearly so that conclusion are
obviously based on reliable results. 4 You may have to consider whether the patient is allergic to any
of the antibiotics. Label the Petri dish on the base at the edge with your name, the date and the type
of bacterium it is inoculated with. 3 Flame the forceps and then use them to pick up an antibiotic
disc or Mast ring. Brine shrimps are very delicate and care must be taken when handling them.
Reviews Select overall rating (no rating) Your rating is required to reflect your happiness. It does not
attempt to provide detailed accounts of the techniques. For example, you can see if there is a
significant positive or negative correlation between the factor you think is responsible for the pattern
and the distribution of the organisms you have recorded.
Show replies PMTEducation 2 years ago Glad it's helped! - Heidi, PMT team Hide replies Empty
reply does not make any sense for the end user Submit reply Cancel Bruffalo 4 years ago report 5
Amazing, really helpful for my students who have moved schools and missed sections, made it a lot
easier to catch them up in short sessions. Three-way taps can cause confusion with some students.
The data can be subjected to statistical analysis using correlation coefficients (Pearson’s parametric or
Spearman rank non-parametric), particularly useful with transects. When the lid moves up and down,
the string moves over the pulley. Sensors such as motion sensors can be used to record the up and
down motion of the airfilled spirometer lid. Finding the horizontal scale 4 On most kymographs there
is a switch allowing you to set the speed at which the drum turns. Review 4 Select overall rating (no
rating) Your rating is required to reflect your happiness. Read the following section, which briefly
mentions some of the techniques that you could use. If snails are still inactive, they can be paced for
a short time in warm water. Question 1 can be used as a summary activity for student understanding
of respiration if students are asked to complete it in as much detail as possible and to state the source
of carbon dioxide as well as its fate. Learn how we and our ad partner Google, collect and use data.
I had a plan written by my teacher Posted from TSR Mobile 0 Report Reply Reply 11 9 years ago
Watchthe 5 Original post by Changing Skies Hey. Atmospheric pressure pushes the liquid along the
tube, until the pressure in and outside the tube is equal. A transect is effectively a line laid out across
the habitat, usually using a tape measure, along which samples are taken. You need to be consistent
when determining presence or absence in a sampling unit. It helps if the whole group can have access
to a computer as soon as possible after collecting the data and to a full set of equipment during data
collection. They are easy to keep in a glass or plastic aquarium tank containing several centimetres of
compost, organic compost is recommended. And I did well on it apart from the method section, I am
very confused about my method, do I just outline a treatment of colic and use a study to show that
vets use it and show that it is effective. The two sites could be compared by random sampling. Do
NOT open the Petri dishes once they have been incubated. As the lid moves up and down the change
in distance can be recorded. Community ? Groups Quotes Ask the Author People Sign in Join Jump
to ratings and reviews Want to read Buy on Amazon Rate this book Edexcel A Level Science: A2
Biology Students' Book with ActiveBook CD Ann Fullick 3.50 38 ratings 2 reviews Want to read
Buy on Amazon Rate this book Description Providing complete,step-by-step coverage of the 2008
Edexcel GCE Biologyspecification, this is the official Student Book for theconcept-led approach to
the specification. To investigate the effect of exercise, readings should be taken immediately after
exercise. Error: Try Again Ok Sell Watchlist Expand Watch List Loading. The conductivity of pure
water is zero; increasing ion concentration raises the conductivity. I just wanted all the past papers
for Edexcel as i can prepare from now for the exam! 0 Report Reply Reply 9 9 years ago Watchthe 5
Hey thanks so much for uploading this. The data collected can then be made available to students
involved in the investigation. Write a review Update existing review Submit review Cancel It's good
to leave some feedback. See other similar resources ?0.00 5.00 2 reviews Download Save for later
Not quite what you were looking for. The abundance of organisms within the quadrat is estimated
(see the section Methods of measuring abundance and Figure 3).