Scribd Logo
Upload

Welcome to Scribd!

  • 6 views

    Doxycycline Tablets

    Uploaded by

    Alexandra Cociu
    This document provides two typical chromatograms for the analysis of doxycycline tablets. The first chromatogram is for the related substances test and shows 5 peaks corresponding to impurities and doxycycline. The second chromatogram is for the assay test and shows a single peak for doxycycline. Both chromatograms use the same column, mobile phase, and detection method at 280nm. The chromatograms are intended to guide analysts in interpreting results for doxycycline tablet tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Doxycycline Tablets

    Uploaded by

    Alexandra Cociu
    6 views2 pages
    This document provides two typical chromatograms for the analysis of doxycycline tablets. The first chromatogram is for the related substances test and shows 5 peaks corresponding to impurities and doxycycline. The second chromatogram is for the assay test and shows a single peak for doxycycline. Both chromatograms use the same column, mobile phase, and detection method at 280nm. The chromatograms are intended to guide analysts in interpreting results for doxycycline tablet tests.

    Original Description:

    doxiciclina tablets

    Original Title

    Doxycycline-Tablets (1)

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides two typical chromatograms for the analysis of doxycycline tablets. The first chromatogram is for the related substances test and shows 5 peaks corresponding to impurities and doxycycline. The second chromatogram is for the assay test and shows a single peak for doxycycline. Both chromatograms use the same column, mobile phase, and detection method at 280nm. The chromatograms are intended to guide analysts in interpreting results for doxycycline tablet tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    6 views2 pages

    Doxycycline Tablets

    Uploaded by

    Alexandra Cociu
    This document provides two typical chromatograms for the analysis of doxycycline tablets. The first chromatogram is for the related substances test and shows 5 peaks corresponding to impurities and doxycycline. The second chromatogram is for the assay test and shows a single peak for doxycycline. Both chromatograms use the same column, mobile phase, and detection method at 280nm. The chromatograms are intended to guide analysts in interpreting results for doxycycline tablet tests.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    Doxycycline Tablets – BP 2019

    These chromatograms are provided for information only as an aid to analysts and are intended as
    guidance for the interpretation and application of BP monographs.

    Typical chromatogram for solution (3) in the Related Substances test for Doxycycline Tablets as
    published in BP 2019.
    12 12

    10 10

    8 8

    6 6
    mAU

    mAU
    4 1 4

    2 2

    0 0

    4
    0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44
    Minutes

    Peak ID: 1: Impurity C; 2: Impurity A; 3: Impurity B; 4: Doxycycline; 5: Impurity F.

    Column : Waters, XTerra RP18 (250 mm x 4.6 mm, 5 µm)


    Solution A : 11.16 % w/v solution of ethylenediaminetetracetic acid disodium salt dihydrate,
    adjusted to pH 7.0 with concentrated ammonia (35 %).
    Buffer : 6.79 % w/v tetrabutylammonium hydrogen sulfate solution adjusted to pH 7.0
    with concentrated ammonia (35 %).
    Mobile Phase : Acetonitrile: water: buffer: solution A (13:17:35:35, v/v/v/v)
    Diluent : 0.01 M hydrochloric acid
    Flow Rate : 1.0 mL/min
    Column Temp : 35°C
    Injection Volume : 20 µL
    Detection : UV, 280 nm
    Typical chromatogram for solution (2) in the Assay test for Doxycycline Tablets as published in BP
    2019.
    35 35

    30 30

    25 25

    20 20
    mAU

    mAU
    15 15

    10 10

    5 5

    0 0

    1
    0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
    Minutes

    Peak ID: 1: Doxycyline

    Column : Waters, XTerra RP18 (250 mm x 4.6 mm, 5 µm)


    Solution A : 11.16 % w/v solution of ethylenediaminetetracetic acid disodium salt dihydrate,
    adjusted to pH 7.0 with concentrated ammonia (35 %).
    Buffer : 6.79 % w/v tetrabutylammonium hydrogen sulfate solution adjusted to pH 7.0
    with concentrated ammonia (35 %).
    Mobile Phase : Acetonitrile: water: buffer: solution A (13:17:35:35, v/v/v/v)
    Diluent : 0.01 M hydrochloric acid
    Flow Rate : 1.0 mL/min
    Column Temp : 35°C
    Injection Volume : 20 µL
    Detection : UV, 280 nm

    You might also like