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    PhysioEx Exercise 8 Activity 4

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    andrea gomez herrera
    This document summarizes the results of an experiment that assessed the activity of the enzyme pepsin at different pH levels. The experiment found that pepsin had the highest activity at pH 2.0, which correlates with the pH in the human stomach where protein digestion begins. Negative control tubes without pepsin or substrate validated the experimental results. Higher optical density readings indicated greater substrate digestion by pepsin and thus higher enzyme activity.

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    PhysioEx Exercise 8 Activity 4

    Uploaded by

    andrea gomez herrera
    25 views3 pages
    This document summarizes the results of an experiment that assessed the activity of the enzyme pepsin at different pH levels. The experiment found that pepsin had the highest activity at pH 2.0, which correlates with the pH in the human stomach where protein digestion begins. Negative control tubes without pepsin or substrate validated the experimental results. Higher optical density readings indicated greater substrate digestion by pepsin and thus higher enzyme activity.

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    This document summarizes the results of an experiment that assessed the activity of the enzyme pepsin at different pH levels. The experiment found that pepsin had the highest activity at pH 2.0, which correlates with the pH in the human stomach where protein digestion begins. Negative control tubes without pepsin or substrate validated the experimental results. Higher optical density readings indicated greater substrate digestion by pepsin and thus higher enzyme activity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    25 views3 pages

    PhysioEx Exercise 8 Activity 4

    Uploaded by

    andrea gomez herrera
    This document summarizes the results of an experiment that assessed the activity of the enzyme pepsin at different pH levels. The experiment found that pepsin had the highest activity at pH 2.0, which correlates with the pH in the human stomach where protein digestion begins. Negative control tubes without pepsin or substrate validated the experimental results. Higher optical density readings indicated greater substrate digestion by pepsin and thus higher enzyme activity.

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    © All Rights Reserved
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    22/2/24, 16:59 PhysioEx Exercise 8 Activity 3

    PhysioEx Lab Report


    Exercise 8: Chemical and Physical Processes of Digestion
    Activity 3: Assessing Pepsin Digestion of Protein
    Name: ejercicio 8.3
    Date: 22 February 2024
    Session ID: session-36aab3be-9bf6-7587-d13c-38cc6e9c89b3

    Pre-lab Quiz Results


    You scored 100% by answering 5 out of 5 questions correctly.

    1 Where in the body does protein digestion begin?

    You correctly answered: the stomach.

    2 The substrate for pepsin is

    You correctly answered: protein and peptides.

    3 In this activity the substrate you will be using to detect protein digestion is

    You correctly answered: BAPNA.

    4 Negative results with the negative controls

    You correctly answered: are expected and validate the experiment.

    5 A spectrophometer measures

    You correctly answered: optical density.

    Experiment Results
    Predict Question
    1 Predict Question: At which pH do you think pepsin will have the highest activity?

    Your answer: pH 2.0.

    Stop & Think Questions


    1 Which of the following tubes are negative control tubes?

    You correctly answered: tubes 3 (pepsin, deionized water, pH 2.0 buffer) and 4 (deionized
    water, BAPNA, pH 2.0 buffer).

    2 An optical density greater than zero indicates that

    Your answer: pepsin is not active.

    Correct answer: BAPNA digestion has occurred and pepsin is active. i


    physioex.pearson.es.ezbusc.usc.gal/exercise-8/ex08act03/ 1/3
    22/2/24, 16:59 PhysioEx Exercise 8 Activity 3

    Experiment Data
    Tube Reagent 1 Reagent 2 Reagent 3 Boiled Time Temp Optical
    No. (min) (°C) Density
    pH 2.0
    1 Pepsin BAPNA + 60 37 0.00
    Buffer
    pH 2.0
    2 Pepsin BAPNA - 60 37 0.40
    Buffer
    Deionized pH 2.0
    3 Pepsin - 60 37 0.00
    Water Buffer
    Deionized pH 2.0
    4 BAPNA - 60 37 0.00
    Water Buffer
    pH 7.0
    5 Pepsin BAPNA - 60 37 0.03
    Buffer
    pH 9.0
    6 Pepsin BAPNA - 60 37 0.00
    Buffer

    Post-lab Quiz Results


    You scored 100% by answering 4 out of 4 questions correctly.

    1 Pepsin would be most active

    You correctly answered: in the stomach.

    2 Which two tubes validated the results of the experiment?

    You correctly answered: tubes 3 (pepsin, deionized water, pH 2.0 buffer) and 4 (deionized
    water, BAPNA, pH 2.0 buffer).

    3 With more enzyme activity the optical density

    You correctly answered: increased.

    4 If pepsin were digesting an actual protein substrate, the product would be

    You correctly answered: peptides and amino acids.

    Review Sheet Results


    1 Describe the effect that boiling had on pepsin and how you could tell that it had that
    effect.

    Your answer:

    El someter la enzima a calor provocó que esta se desnaturalizara y por lo tanto no


    pudiera hidrolizar la BAPNA en péptidos y aminoácidos.

    2 Was your prediction correct about the optimal pH for pepsin activity? Discuss the
    physiological correlation behind your results. i
    physioex.pearson.es.ezbusc.usc.gal/exercise-8/ex08act03/ 2/3
    22/2/24, 16:59 PhysioEx Exercise 8 Activity 3

    Your answer:

    Como se predijo, la mayor actividad de la enzima es a pH 2, que es el pH normal del


    estómago y donde la actividad normalmente en un individuo.

    3 What do you think would happen if you reduce the incubation time to 30 minutes for
    tube 5 (pepsin, BAPNA, pH 7.0 buffer)?

    Your answer:

    Probablemente al haberle dado menos tiempo y tener un pH distinto del que


    normalmente está acostumbrado a actuar, provocaría una menor densidad óptica ya que
    no le daría tiempo a hidrolizar tanto sustrato como en 60 minutos o incluso no le daría
    tiempo a a hidrolizar nada.

    i
    physioex.pearson.es.ezbusc.usc.gal/exercise-8/ex08act03/ 3/3

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