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    Lab 6 FIS3203-PO4

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    Teoh Yying
    This document describes a method for determining orthophosphate concentration in water samples. The method involves reacting the water sample with a mixture of reagents containing molybdate, sulfuric acid, ascorbic acid, and potassium antimonyl-tartrate. This results in the formation of a blue complex, the absorbance of which is measured at 880 nm. A standard curve is prepared using known concentrations of orthophosphate standards. The concentration of orthophosphate in samples is then determined using the standard curve and an F-factor calculation.

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    Lab 6 FIS3203-PO4

    Uploaded by

    Teoh Yying
    18 views4 pages
    This document describes a method for determining orthophosphate concentration in water samples. The method involves reacting the water sample with a mixture of reagents containing molybdate, sulfuric acid, ascorbic acid, and potassium antimonyl-tartrate. This results in the formation of a blue complex, the absorbance of which is measured at 880 nm. A standard curve is prepared using known concentrations of orthophosphate standards. The concentration of orthophosphate in samples is then determined using the standard curve and an F-factor calculation.

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    Lab_6_FIS3203-PO4(5) (2)

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    This document describes a method for determining orthophosphate concentration in water samples. The method involves reacting the water sample with a mixture of reagents containing molybdate, sulfuric acid, ascorbic acid, and potassium antimonyl-tartrate. This results in the formation of a blue complex, the absorbance of which is measured at 880 nm. A standard curve is prepared using known concentrations of orthophosphate standards. The concentration of orthophosphate in samples is then determined using the standard curve and an F-factor calculation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    18 views4 pages

    Lab 6 FIS3203-PO4

    Uploaded by

    Teoh Yying
    This document describes a method for determining orthophosphate concentration in water samples. The method involves reacting the water sample with a mixture of reagents containing molybdate, sulfuric acid, ascorbic acid, and potassium antimonyl-tartrate. This results in the formation of a blue complex, the absorbance of which is measured at 880 nm. A standard curve is prepared using known concentrations of orthophosphate standards. The concentration of orthophosphate in samples is then determined using the standard curve and an F-factor calculation.

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    © All Rights Reserved
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    Determination

     of  Orthophosphate  

    FAKULTI AGROTEKNOLOGI DAN SAINS MAKANAN


    UNIVERSITI MALAYSIA TERENGGANU

    FIS 3203
    Water Quality Management for Aquaculture

    Determination of Orthophosphate

    Hii,  Y.  S.  2009  


    Determination  of  Orthophosphate  

    INTRODUCTION

    Phosphates that respond to colorimetric tests on sample without preliminary hydrolysis

    or oxidative digestion of the sample are termed as 'reactive phosphates". Reactive

    phosphate consists largely of ortho-phosphate, with a small fraction of condensed

    phosphate such as di-phosphate and tri-phosphate. In this method, the water sample is

    allowed to react with a composite reagent containing molybdate acid, ascorbic acid anti

    trivalent antimony. The resulting complex is reduced to a blue solution, which is

    measured at 880 nm.

    Reagents

    KH2PO4 standard solution: (0 – 1.0 mg PO4/L).

    Ammonium molybdate solution: Dissolve 4.0 g ammonium paramolybdate,

    (NH4)6Mo7O2.4H2O in 100 ml distilled water.

    Acid Sulfuric solution (5 N): Add 140 ml concentrated sulfuric acid (sp. gr. 1.82) in 1 L

    volumetric flask contained 800 ml of distilled water. Top up the solution to 1 L.

    Ascorbic acid solution (0.01 M): Dissolve 1.76 g ascorbic acid, C6H8O6 in 100 ml

    distilled water. Freshly prepare prior to use.

    Hii,  Y.  S.  2009  


    Determination  of  Orthophosphate  

    Potassium antimonyl-tartrate solution: Dissolve 1.3715 g potassium antimonyl-

    tartrate, K(SbO)C4H6O6.½ H2O in 500 ml distilled water.

    Mix reagent solution. Mix together 75 ml ammonium molybdate solution, 250 ml sulfuric

    acid solution, 150 ml ascorbic acid and 25 ml potassium antimonyl-tartrate solutions.

    Prepare this reagent when needed and discard any excess. Do not store.

    Procedure

    1. Prepare different concentrations of standard solution, 0.05, 0.10, 0.03, 0.05, 1.00

    mg/L PO43-.

    2. Filter all water samples prior to analysis.

    3. Pipette 5.0 ml of each standard solution, water sample and control (distilled water)

    into test tubes. Add 0.5 ml mix reagent to each test tube and stir homogenously.

    4. After 1 hour, record Absorbance using spectrophotometer at wavelength 880 nm.

    Hii,  Y.  S.  2009  


    Determination  of  Orthophosphate  

    Determination of orthophosphate concentration.

    Standard curve

    Prepare 100 ml of each standard solution using the standard solutions (Blank, 0.05,

    0.10, 0.03, 0.05, 1.00 mg/L PO43-)1. Use deionized water or artificial seawater (depend

    on sample) for dilution.

    F factor

    Concentration (mg/ l) = Sample absorbance x F factor

    Factor, F = 1.0 mg/l / (Es-Eb)

    Where,

    Es = absorbance of standard solution

    Eb = blank solution absorbance

                                                                                                                             
    1
     Perform  a  series  of  dilution  instead  of  one,  the  dilution  factor  should  not  exceed  100  x    

    Hii,  Y.  S.  2009  

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