WIX-midiDNA Multipurpose Horizontal Electrophoresis Cell

Instruction Manual

WIX TECHNOLOGY BEIJING CO.,LTD

 Content

Chapter 1 Introduction.................................................................................................. 1

1.1 Brief introduction............................................................................................... 1

1.2 Structure.............................................................................................................. 1

1.3 Main technical parameter................................................................................. 2

Chapter 2 Operation procedure....................................................................................2

Chapter 3 Maintenance..................................................................................................3

Chapter 4 Trouble shooting........................................................................................... 4

Chapter 5 Transportation and storage.........................................................................6

Chapter 6 Quality guarantee.........................................................................................6

Attachment (for your reference)................................................................................... 7

Proper operation procedures!

Please read carefully before usage!

Please carefully read this instruction manual which contains the proper operation procedures.

Please make the instrument power off while not in the usage in order to avoid shock hazard .Please check the

condition of instrument completely before the usage in the aspect of body crack, body damage, loosed connection,

rubber damage, wire corrosion, wire disconnection, electricity leakage, buffer leakage in order to guarantee the

smooth operation, please discontinue the usage and report to WIX or local agency immediately in case of any

phenomenon mentioned above.

Note: We will not bear the responsibility for any result caused by any improper usage.

Statement: It is not used in the clinical test for it is the instrument for scientific research and teaching.
Chapter 1 Introduction
1.1 Brief introduction
WIX-midiDNA Multipurpose Horizontal Electrophoresis Cell equipped with the function of adding sample by
pipette is mainly used for electrophoresis of the agarose gel of DNA and RNA, whose special-used gel casting
bench and flexible combination of tray with ear-shaped structure are convenient to be used. It can conduct the
experiment of 96-hole PCR sample electrophoresis with different volume of agarose and size like 6.5×6.5 cm,
6.5×13 cm, 13×6.5 cm, 13×13 cm etc. The instrument mainly consists of gel tray, lower body, upper body,
gel-making kit, comb etc.

1.2 Structure
Prior to usage, please check the accessories according to the packing list and the condition of instrument. Please

contact the head quarter or local agency in case of any discrepancy.

Please refer to the following packing list:

Accessory Quantity Accessory Quantity

Upper lid and power
Main body 1 piece 1 set
supply wire
Electrode 1 pair Gel-making frame 1 piece
5 pieces 9 pieces
13×13cm, 1 piece 1 piece, 7+7/14 wells, 0.75mm thickness
13×6.5 cm, 1 piece 1 piece, 9+9/19 wells, 0.75mm thickness
Gel tray 6.5×13cm, 1 piece Comb 4 pieces, 12+12/27 wells, 1.0mm thickness
6.5×6.5cm, 2 pieces 1 piece, 7+7/14 wells, 1.5mm thickness
1 piece, 9+9/19 wells, 1.5mm thickness
1 piece, 3+3/3+2 wells, 2.0mm thickness
Qualification
Instruction manual 1 set 1 piece
certificate

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1.3 Main technical parameter

Size 300×170×80mm

Standard: 13×13cm, 13×6.5cm,

Tray size (W*L)
6.5×13cm, 6.5×6.5cm

7+7/14 wells, 0.75mm thickness

9+9/19 wells, 0.75mm thickness

Comb
12+12/27 wells, 1.0mm thickness

7+7/13 wells, 1.5mm thickness

9+9/19 wells, 1.5mm thickness

3+3/3+2 wells, 2.0mm thickness

Quantity of gel to be made 1-4 piece(s)

Buffer 1000ml

Net weight 1.1kg

The instrument requires direct current, the followings are the specific:
The maximum voltage: 200V
The maximum power: 40W
The maximum buffer temperature: 40℃

Chapter 2 Operation procedure

1. Put the gel-making frame on the horizontal desk and put the gel tray into the grid of gel-making frame
accordingly then put the comb in the narrow slot.4 types of gel can be made in the gel-making frame like 13×13 cm,
13×6.5 cm, 6.5×13 cm, 6.5×6.5 cm according to the actual needs.

2. Make the agarose solution with proper concentration by electrophoresis buffer according to the size of separated
DNA fragment: measure the dry powder of agarose accurately and put it in the conical flask or the glass bottle with
fixed volume of electrophoresis buffer, and then use the glass stick to stir it evenly and put it into the boiling water
or microwave oven for being heated until the agarose is fused (to determine the concentration of agarose according
to the attached list).

3. Put the gel into the gel tray slowly while it is slightly cooled, the ideal thickness of gel is 3～5mm (Note: avoid
the bubble in the gel).

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4. Let the gel coagulates completely for 30～45min in room temperature (The coagulation period can also be
shortened by putting it in the 4℃ refrigerator after the gel coagulates slightly). Take out the comb carefully and put
gel in the cell, the side of hole is close to cathode (black end).

5. Put the buffer in the electrophoresis cell and keep the surface of buffer at least 2mm higher than the gel (Note:
TAE buffer should be replaced after 2 to 3 times, the TBE buffer can be used for around 10 times).

6. Mix proper amount of DNA sample and 10×buffer (analyze single DNA sample, such as L bacteriophage or
plasmid DNA, each sample-adding hole with width of 5mm is suitable for 100～500ng DNA. The resolution is not
decreased obviously when 20～30μg is added if sample consists of many DNA fragments, such as DNA enzyme
digestion sample of mammal). Use the pipette to add the sample with proper amount of standard DNA molecular
weight into the right side hole and left side hole.

7. Lid the electrophoresis cell after sample adding and power on by 5～8V/cm, the distance in which should be
matched with the measured distance between anode and cathode. The bubble is created by electrolytic action. DNA
migrates to the anode (red plug).The period of electrophoresis is determined by the length of gel voltage, and the
size of DNA fragment. The longer the gel is, the lower the voltage is, the bigger the DNA fragment is, the more
time required. However the resolution of big DNA fragment is very low and the band is not clear if the high voltage
is adopted (The voltage per centimeter of gel is less than 8V because the high voltage causes the lower resolution.
The electrophoresis migration rate of linear DNA molecular is increased as voltage rises accordingly only in the
lower voltage.).

8. When indicator migrates to the bottom of gel, power off and take out sample and put it in the EB solution for
being dyed for 5～10min (EB will be resolved in the sunshine and should be stored in the dark room),Observe the
sample in UV Transilluminator and take photo if necessary (EB can be put in the gel during the gel-making
process).

Chapter 3 Maintenance
1. Operation temperature: the temperature is 4~40℃, the relative humidity is less than 95%, good ventilation and
no erosive air.
2. Please use the soft decontaminant to cleanse carefully the gel tray, lower body, gel-making kit and comb.
3. In order to avoid the rust, please use bibulous paper to dry the electrode tip once it is wetted.
4. In order to avoid the damage and corrosion, please keep the electrophoresis cell clear of acid solution and
aqueous alkali.

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Chapter 4 Trouble shooting
Trouble description Analysis Solution Remark
Avoid nuclease pollution during the
DNA degradation
process of experiment.
Renew the electrophoresis buffer.
If the electrophoresis buffer is used
The electrophoresis
many times, the ionic strength is
buffer is used more
lowered, the pH value is decreased,
times.
buffer efficiency is lowered,Which
affects the electrophoresis.
While being during the
electrophoresis, the voltage should
be less than 8V/cm, the temperature
should less than 30℃. In case of
The condition of
huge DNA electrophoresis, the
electrophoresis is not
temperature should be less than
suitable.
15℃. Check whether the
electrophoresis buffer is available
Vague DNA band enough to conduct the
electrophoresis.
Over-volume of DNA
Reduce the volume of DNA sample.
sample.
Remove the surplus salt via ethyl
DNA sample with high
alcohol precipitation before the
volume of salt.
electrophoresis.
Protein pollution Remove the protein via phenol.
No heating before electrophoresis,
DNA denaturation dilute the DNA via 20mM NaCl
buffer

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Trouble description Analysis Solution Remark
Should be heated for 5 minutes
Recovery feature of cos
under temperature of 65℃ and be
position λ/Hind Ⅲ
cooled for 5 minutes on the ice
fragment .
before electrophoresis.
While being during the
Irregular migration of The condition of electrophoresis, the voltage should
DNA band. electrophoresis is not be less than 8V/cm,the temperature
suitable. should less than 40℃. Renew the
electrophoresis buffer frequently.
No heating before electrophoresis,
DNA denaturation dilute the DNA via 20mM NaCl
buffer.
Increase the volume of DNA
Not enough DNA sample
sample.
DNA degradation Avoid nuclease pollution during the
process of experiment.
Unclear band or no DNA Shortening the electrophoresis
band. DNA migrate out of gel. period, lowering the voltage,
increase the concentration of gel.
The light source is not The ultraviolet source with short
suitable for the DNA that wave light (254mm) should be
is polluted by EB. adopted.
Shortening the electrophoresis
Small-sized DNA
period, lowering the voltage,
migrates out of gel.
increase the concentration of gel.
Hardly recognition of Prolonging the period of
DNA with similar sized electrophoresis and use the gel with
molecule. proper concentration.
Disappearance of DNA Do not heat DNA chain with high
band temperature before electrophoresis,
DNA denaturation
dilute the DNA via 20mM NaCl
buffer
DNA china is huge,
normal gel Conduct the analysis in pulse gel
electrophoresis is not electrophoresis.
suitable.

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Trouble description Analysis Solution Remark
The gel should be solidified at least
The gel is not solidified
30-40 minutes.
Channel of sample is not completely, the comb is
Check the comb.
straight. slanting, there is bubble
Avoid the bubble during the process
in the gel
gel-making.
The band of high
molecular weight is clear Using the gel with proper
The concentration of gel
and beautiful while the concentration.
is low.
band of low molecular Use acrylamide to separate the gel.
weight is scattered.
Choose the most suitable voltage.
High frequency usage of buffer or
The gel is melted. High temperature
the content is wrong, the buffer has
to be re-formulated.
Reduce the concentration of salt in
The concentration of salt
the sample.
is high.
Lower the voltage or re-formulate
High temperature,
The band of sample is the buffer.
Over amount of sample
scattered. Make the gel thicker or choose the
DNA degradation
suitable sample,
Sample is ruptured
Re-extract the sample.
Re-make the gel.

Chapter 5 Transportation and storage

1. Please handle the instrument carefully and lightly during the transportation and storage and avoid the
heavy-object bearing.
2. Packaged instrument should be stored in the room with temperature -20℃~55℃ and humidity less than 93%,
without erosive air and with good ventilation.

Chapter 6 Quality guarantee

(1) The warranty is 2 years since the date of sales.
(2) The warranty excludes the following situations otherwise it is charged.
a. No presentation of warranty card and invoice.
b. The invoice is revised.
c. Improper operation or accident factors.

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 d. The damage is caused by the user’s repair.
e. Out of the warranty, the instrument is still in usage after repair.

Attachment (for your reference)

Concentration of arose gel (ratio of weight to The size of recognizable linear DNA fragment
volume) (kb)
0.4 % 5~60
0.7 % 0.8~10
1.0 % 0.4~6
1.5 % 0.2~4
1.75 % 0.2~3
2.0 % 0.1~3

WIX TECHNOLOGY（BEIJING）CO.,LTD
Tel: +86 010-89797600
E-mail: sales@wixscientific.com
Website: http://www.wixscientific.com/
Add: No. 10 Building Dongliantongchuang Sci-Tech Park, No.738 Changliu
Road Machikou Town Changping District Beijing People's Republic of China
Postcode: 102202