Cambridge IGCSE™

* 2 1 5 3 8 3 6 5 4 2 *

BIOLOGY 0610/52
Paper 5 Practical Test May/June 2022

1 hour 15 minutes

You must answer on the question paper.

You will need: The materials and apparatus listed in the confidential instructions

INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.

INFORMATION
● The total mark for this paper is 40.
● The number of marks for each question or part question is shown in brackets [ ].

For Examiner’s Use

Total

This document has 12 pages. Any blank pages are indicated.

DC (RW/FC) 303952/4
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1 You are going to investigate the effect of temperature on the diffusion of vitamin C.

Vitamin C is an important part of a balanced diet and is found in some fruits and vegetables. When
vegetables are boiled in water the vitamin C diffuses out into the surrounding water.

A dialysis tubing bag filled with a vitamin C solution represents a vegetable.

The blue dye DCPIP is used as an indicator for the presence of vitamin C. High concentrations of
vitamin C will decolourise DCPIP quickly.

Read all the instructions but DO NOT CARRY THEM OUT until you have drawn a table for
your results in the space provided in 1(a)(ii).

You should use the safety equipment provided while you are carrying out the practical work.

Step 1 Label one large test‑tube hot and a second large test‑tube cold.

Step 2 Remove one piece of dialysis tubing from the beaker labelled D and rub the unknotted
end between your fingers to open it.

Step 3 Use a syringe to put 10 cm3 of the vitamin C solution, labelled V, into the open end of the
dialysis tubing bag.

Step 4 Rinse the outside of the filled dialysis tubing bag by dipping it into the beaker of distilled
water labelled W.

Step 5 Place the filled dialysis tubing bag into the large test‑tube labelled hot and secure it in
place with an elastic band, as shown in Fig. 1.1.

elastic band holding the

open end of the dialysis
tubing bag in place

large test-tube
dialysis tubing bag
containing 10 cm3
of vitamin C solution

Fig. 1.1

Step 6 Repeat step 2 to step 5, placing the second filled dialysis tubing bag into the large
test‑tube labelled cold.

Step 7 Raise your hand when you are ready for hot water to be added to the beaker labelled
hot water.

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Step 8 Measure the temperature of the water in the beaker labelled hot water and record this in
1(a)(i).

Step 9 Measure the temperature of the water in the beaker labelled cold water and record this
in 1(a)(i).

(a) (i) Record the temperature of the hot water and the cold water. Include the unit.

temperature of hot water ...................................

temperature of cold water ...................................

[2]

Step 10 Half‑fill the large test‑tube labelled hot with water from the hot water beaker.

Step 11 Half‑fill the large test‑tube labelled cold with water from the cold water beaker.

Step 12 Start the stop‑clock and leave the dialysis tubing bags in the water for 15 minutes.

Continue with the other questions while you are waiting.

Step 13 Label an empty small beaker hot and another empty small beaker cold.

Step 14 After 15 minutes, remove the dialysis tubing bag from the large test‑tube labelled hot
and put it in the container labelled waste.

Step 15 Pour the remaining contents of the large test‑tube labelled hot into the small beaker
labelled hot.

Step 16 Repeat step 14 and step 15 with the large test‑tube labelled cold and the small beaker
labelled cold.

Step 17 Fill a clean syringe with 10 cm3 of DCPIP solution.

Step 18 Put 1 cm3 of the solution from the small beaker labelled hot into a clean test‑tube.

Step 19 Add a drop of the DCPIP solution to the test‑tube from step 18 and swirl to mix. After a
few seconds the blue colour should disappear.

Step 20 Continue to add drops of DCPIP until the blue colour remains after mixing.

Step 21 Calculate and record, in your table in 1(a)(ii), the volume of DCPIP used.

Step 22 Repeat step 17 to step 21 with the solution in the small beaker labelled cold.

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(ii) Prepare a table to record your results in the space provided.

The volume of DCPIP used can be calculated using the equation:

volume of DCPIP used = 10 – volume of DCPIP remaining in the syringe

[4]

(iii) State a conclusion for your results.

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..................................................................................................................................... [1]

(iv) Suggest why the dialysis tubing bag was rinsed in step 4.

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(v) Identify one source of error in step 10 or step 11 and suggest a suitable piece of
equipment to overcome this error.

error ...................................................................................................................................

...........................................................................................................................................

equipment .........................................................................................................................

...........................................................................................................................................
[2]

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(vi) Identify the variable that you changed (independent variable) and the variable that you
measured (dependent variable) in this investigation.

independent variable .........................................................................................................

...........................................................................................................................................

dependent variable ............................................................................................................

...........................................................................................................................................
[2]

(vii) Suggest why repeating the procedure several times would improve the investigation.

...........................................................................................................................................

...........................................................................................................................................

..................................................................................................................................... [1]

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(b) Dialysis tubing acts as a partially permeable membrane and can be used to represent a
model cell to investigate osmosis.

Plan an investigation to find out how different concentrations of sugar solutions affect the
movement of water into or out of dialysis tubing.

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............................................................................................................................................. [6]

[Total: 19]

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2 Nautiluses are a genus of marine animals that live in shells. Fig. 2.1 is a photograph of a nautilus
shell.

A B

Fig. 2.1

(a) (i) Make a large drawing of the shell shown in Fig. 2.1.

[4]
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(ii) Line AB represents the width of the nautilus shell.

Measure the length of line AB in Fig. 2.1.

length of line AB in Fig. 2.1 ........................................ mm

The actual width of the shell is 130 mm.

Calculate the magnification of the shell in Fig. 2.1.

length of line AB in Fig. 2.1
magnification =
actual width of the shell
Give your answer to two significant figures.

Space for working.

................................................................
[3]

(b) Fig. 2.2 shows a fossilised nautilus shell.

Fig. 2.2

Describe one visible similarity and one visible difference between the nautilus shell in Fig. 2.1
and the fossilised nautilus shell in Fig. 2.2.

similarity ....................................................................................................................................

...................................................................................................................................................

difference ..................................................................................................................................

...................................................................................................................................................
[2]

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(c) A population of one species of nautilus was studied. The widths of the nautilus shells were
measured and recorded.

The results are shown in Table 2.1.

Table 2.1

width of shell / mm number of shells

101–110 8
111–120 84
121–130 138
131–140 98
141–150 22

(i) Plot a histogram on the grid of the data in Table 2.1.

[4]

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(ii) Using the information in your graph, describe the results of this study.

...........................................................................................................................................

...........................................................................................................................................

...........................................................................................................................................

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..................................................................................................................................... [2]

(iii) The study measured the width of 350 nautilus shells.

Suggest why such a large number of shells were measured.

...........................................................................................................................................

...........................................................................................................................................

..................................................................................................................................... [1]

(iv) Using the data in Table 2.1, calculate the percentage of the population of nautiluses that
have shells that are wider than 130 mm.

Give your answer to one decimal place.

Space for working.

.............................................................%
[3]

(d) The nautilus feeds on fish which are an important source of protein.

State the name of the test for protein. Give the result of a positive test.

test for protein ...........................................................................................................................

positive test result .....................................................................................................................

[2]

[Total: 21]

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© UCLES 2022 0610/52/M/J/22