Blood Pictures

BIOL 213 Online Lab PowerPoint

Hint: Slides with colored backgrounds help to divide content into

different days.

The use of Chrome as your internet browser is recommended for

this course. If you do not have it, you can download it here.
Normal Blood Histology(Blood Cell
Identification)
 Use the following pictures to help you
identify terms from the lab term handout.
***You always only need to know the terms listed in the lab term handout!Another good resource for histology is

the Olexik website: http://faculty.montgomerycollege.edu/wolexik/204_histology_page.htm

Figure 17.2b Blood cells. Erythrocyt Platelets Identify Leukocytes on
es slides 4-10.
Slide #4 can serve as
your “guide/key” for identifying
Leukocytes; ask your professor
if you get “stuck”!

Neutroph
il

Eosinoph
il

Monocyt Lymphocy
e te
Photomicrograph of a human blood smear,
Wright’s stain (610×) © 2016 Pearson Education, Inc.
Figure 17.10 Leukocytes.

Leukocytes (WBCs)
Granulocytes Agranulocytes

Neutrophil Eosinophi Basophil: Lymphocyte Monocyte:

: l: Bilobed (small): Large Kidney-shaped
Multilobed Bilobed nucleus, spherical nucleus,
nucleus, pale nucleus, red purplish-black nucleus, thin abundant pale
red and blue cytoplasmic cytoplasmic rim of pale blue cytoplasm
cytoplasmic granules granules blue cytoplasm
granules

© 2016 Pearson Education, Inc.

https://blog.valdosta.edu/ap2/identification-of-red-w
hite-blood-cells/
 Use the following pictures to help you
practice finding the terms from the lab
term handout on unlabeled images.

Remember, you won’t learn them if you don’t take plenty of time to practice on pictures
with NO labels (including no labels for what type of slide it is on histology pictures)!
Also, be sure to mix up the order once you get comfortable with the unlabeled slides.
Over the weekend, once you are feeling confident with the pictures here, do the histology
quizzes in PAL (from the Pearson website) or Anatomy Drill and Practice (from the Wiley
site) to get practice with new pictures that you haven’t seen.
 Virtual Microscope
Use the virtual microscope to further investigate the blood smear slide:
http://virtualslides.med.umich.edu/Histology/Cardiovascular%20System/081-2_HISTO_40X.svs/view.apm
l?cwidth=860&cheight=733&chost=virtualslides.med.umich.edu&listview=1&title=&csis=1

http://virtualslides.med.umich.edu/Histology/Cardiovascular%20System/081-4_HISTO_40X.svs/view.apm
l?cwidth=860&cheight=733&chost=virtualslides.med.umich.edu&listview=1&title=&csis=1
http://virtualslides.med.umich.edu/Histology/Cardiovascular%20System/081-3_HISTO_40X.svs/view.apm
l?cwidth=860&cheight=733&chost=virtualslides.med.umich.edu&listview=1&title=&csis=1
Easier to identify Eosinophils:
http://virtualslides.med.umich.edu/histology/Cardiovascular%20System/Hematology%20Lab%20Normal1
%2063X.svs/view.apml?X=0&Y=0&zoom=4.77038310412574
Easier to identify Eosinophils, several of Basophils in the right upper-quadrant of the picture:
http://virtualslides.med.umich.edu/Histology/Cardiovascular%20System/Hematology%20Lab%20Normal
%20Smear%2086X%20thick.svs/view.apml?X=-0.00276497289957539&Y=-0.00619880963431794&zoo
m=7.30463170781473
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Virtual Blood Typing Lab: More Images
for Practice
You should have already done the lab (handout provided in the Week 3 Module).
 This plate was treated much like the
different tubes in the activity that you did
online. With antibodies from a lab added 1. What is the blood type?
to an individuals blood in the different 2. What antigens and antibodies are present in this person’s
wells (pictured below). blood?
3. What blood types could this individual receive in a transfusion?
(Don’t forget to address positive(+) vs negative (-)…. Don’t just
say the letter.)

NO Agglutination Review Blood

typing slides 13 & 14.
Answer the questions
and identify the blood
type results;
use your lecture notes
or ask your professor i
f you get “stuck”!
Agglutination
1. What is the blood type?
2. What antigens and antibodies are present in this person’s
blood?
3. What blood types could this individual receive in a transfusion?
(Don’t forget to address positive(+) vs negative (-)…. Don’t just
say the letter.)
Blood Pathology
 Use the following pictures to help you
identify terms from the lab term handout.
***You always only need to know the terms listed in the lab term handout!Another good resource for histology is

the Olexik website: http://faculty.montgomerycollege.edu/wolexik/204_histology_page.htm

***Learn about the different pathologies

for BONUS on the midterm.
 Leukocyte Disorders
Leukemias
Cancerous conditions involving WBCs
Named according to the abnormal WBC clone involved
Acute leukemia (quick advancing) = children
Define
Chronic leukemia (slow advancing) = older people “Leukemia” (slides
17 & 18) & Review
images (Slides 19-
21) for images of
Pay attention to the notes on these slides!!! various kinds of
leukemias; you will
NOT be tested on
slides 19-21
 Leukemia
Bone marrow filled with cancerous leukocytes
Symptoms: fever, infection, weight loss, pain, anemia,
bleeding
Death caused by internal hemorrhage and overwhelming
infections
Treatments include irradiation, antileukemic drugs, and
stem cell transplants
 Eosinophilic Leukemia

Prof. Olexik’s Website

 Lymphocytic Leukemia

Prof. Olexik’s Website

 Myeloblastic Leukemia

Prof. Olexik’s Website

Figure 17.11 Leukocyte formation.
Hematopoietic stem cell
Stem (hemocytoblast)
cells
Examine slide 22
Myeloid stem cell Lymphoid stem cell
and define:
Hemocytoblast,
Neutrophil,
Committe Myeloblas Myeloblas Myeloblas Monoblas B lymphocyte T lymphocyte
Lymphocyte,
Monocyte, Eosinophil,
t precursor precursor
t t t
d
cells Basophil, &
Macrophage. You do
Development Promyelocyte Promyelocyte Promyelocyte Promonocyte
not need to know the
al
other cells in this
pathway
image. But do know
Eosinophili
c
myelocyte
Basophili
c
myelocyte
Neutrophili
c
myelocyte
that Erythrocytes and
platelets are ultimately
derived from cells that
Eosinophili
c
Basophili
c
Neutrophili
c
originate with
band cells band cells band cells
Megakaryocytes

Granular Agranula
r
leukocyte Eosinophil Monocyteleukocyt
Basophil Neutrophil B lymphocytes T
s s s s s es lymphocytes

Some become Some become Some become

Macrophages (tissues) Plasma cells Effector T

cells

© 2016 Pearson Education, Inc.

 Erythrocyte Disorders
Anemia: blood has ↓ O2 carrying capacity
Indication of another disorder
Blood O2 levels cannot support normal metabolism
Symptoms = fatigue, paleness, shortness of breath, and chills

Review slides
23-25 & 38-40 and define the following:
Anemia, Sickle Cell Anemia (Slides 24, 25, 38, 39, 40)
 Sickle Cell Anemia
- Sickle cell anemia is the most
common form of sickle cell
disease (SCD). SCD is a serious
disorder in which the body makes
sickle-shaped red blood cells.
“Sickle-shaped” means that the
red blood cells are shaped like a
crescent.
- Sickle cells contain abnormal
hemoglobin called sickle
hemoglobin or hemoglobin S.
Sickle hemoglobin causes the
cells to develop a sickle, or
crescent, shape.
- Sickle cells are stiff and sticky.
They tend to block blood flow in
the blood vessels of the limbs and Abnormal
organs. Blocked blood flow can Normal
cause pain, serious infections,
and organ damage.

http://www.nhlbi.nih.gov/health/dci/Diseases/Sca/SCA_WhatIs.html - Pathology
(a) Normal
erythrocyte

1 2 3 4 5 6 7 1
4
6

(b) Sickled
erythrocyte

1 2 3 4 5 6 7 1
4
6 Mari
 Uremia (Burr Cells)

- Increased wastes in bloodstream cause

RBC morphology to change.
- The only secondary pathology that we
are studying. Secondary because it is
due to another (more serious)
problem.
- Usually secondary effect of kidney
disease.

OMIT slides 26-37 & 40-47; you will not be

tested on these

Prof. Olexik’s Website

Parasitic Infections
 Trypanosoma

- Flagellated protozoan which

causes African Sleeping
Sickness.
-

- Difficult to control because

organisms repeatedly change
their protective coat.

Prof. Olexik’s Website

 Plasmodium

- Protozoan which causes

Malaria.
-

- Lifecycle directly involves

the RBCs.

Prof. Olexik’s Website

Raven
 Use the following pictures to help you
practice finding the terms from the lab
term handout on unlabeled images.

Remember, you won’t learn them if you don’t take plenty of time to practice on pictures
with NO labels (including no labels for what type of slide it is on histology pictures)!
Also, be sure to mix up the order once you get comfortable with the unlabeled slides.