JEW031 - EN Spectro 6700 Series Manual

67 Series
Spectrophotometers
Operating Manual
670 005/REV A/08-07

67 Series Spectrophotometers
Models 6700, 6705 & 6715
Operating Manual
670 005/REV A/08-07

Safety
This is important information; please read carefully before installing or using this instrument.
1. The 67 Series Spectrophotometers are designed for operation by trained personnel who are
aware of the principles and applications involved. For further help and advice please contact your
local distributor, e-mail sales@jenway.com or visit www.jenway.com
2. The spectrophotmeter is a sensitive electronic and optical instrument designed for use in a
laboratory environment. Careful adherence to the installation instructions must be observed. If in
doubt contact a relevant and competent authority for advice before proceeding.
3. In addition to observing the instructions detailed in the Operating Manual and Service Manual for
this instrument all installation, operating and service personnel must be aware of, and employ, a
safe system of work.
4. Voltage levels hazardous to life are present in this instrument, for personal safety only trained
engineers aware of the risk and avoidance of electric shock should remove protective covers from
the instrument.
5. This instrument is designed for minimal maintenance, which must be carried out carefully following
the procedures detailed in this manual. All safety instructions in these procedures, as well as
those defined locally for the area or environment where the work is being carried out must be
observed.
6. Other than for those items defined in the maintenance procedures herein there are no user
serviceable items in this instrument. Removal of covers and attempted adjustment or service by
unqualified personnel will invalidate any warranty and incur additional charges for repair.
7. Reference should always be made to the Health and Safety Data for any chemicals or reagents
used. All available information, advice and warnings on the handling, storage, use and disposal of
such must be carefully observed. When not available this data must be requested from the
supplier before proceeding in any way.
8. It is important that good laboratory practice is observed when handling samples, chemicals,
reagents and ancillary equipment in order to carry out measurement and analysis with this
instrument. Suitable safety and personal protective equipment must be used at all times.
9. If it is suspected that safety protection has been impaired in any way, the spectrophotometer must
be made inoperative and secured against any intended operation. The fault condition must be
reported to the appropriate servicing authority. In all such reports the model number and
serial number of the spectrophotometer must be quoted.
Guarantee
Please read this important guarantee information:
Notwithstanding the description and specification(s) of the instruments contained in the Operating
Manual, Jenway hereby reserves the right to make changes as it sees fit to the instruments or to any
components of the instruments.
This Manual has been prepared solely for the convenience of Jenway customers and nothing in this
Manual shall be taken as a warranty, condition or representation concerning the description,
merchantability, fitness for purpose, or otherwise of the instruments or components.
The 67 Series spectrophotometers are guaranteed for a period of 3 years from the date of purchase.
Within this period we undertake to supply replacements free of charge for parts that may on
examination prove to be defective, provided that the defect is not the result of misuse, accident or
negligence.
On all correspondence, please quote the model and serial number in full and/or the sales order
number.
Any instrument requiring service under this guarantee should be taken to the supplier through whom it
was purchased, or, in the case of difficulty, it should be carefully packed in its original packaging and
consigned, carriage paid, to us. Jenway takes no responsibility for returned goods damaged in transit.
Returned goods will not be processed without a Returns Authorisation Number.
Call the Service Administrator +44 (0) 1371 820122 for the relevant documentation.
Please write the Returns Number on the outside of any packaging and ensure that a copy of a
Decontamination Certificate is visible.
Please register online (www.jenway.com) or complete and return the Registration Document by fax or
mail.
The Guarantee will be rendered invalid if any specified non-serviceable parts within the instrument are
tampered with (i.e. the monochromator).
Contents
Section 1 Introduction Page

Instrument Description 1.1 1
Instrument Specifications 1.2 2-3
Good Practice Guidelines 1.3 4-5
Section 2 Getting Started

Unpacking 2.1 6-7
Installation 2.2 6-7
Location 2.21 7
Supply voltage 2.22 7
Mains connections 2.23 7
Touch screen functions 2.24 8
Toolbar icons 2.25 9-13
Rear panel layout 2.26 14
Power on and self tests 2.27 15
Section 3 Systems of Operation

Multi-User and Free Operation 3.1 16
Memory Structure 3.2 16
Memory selection 3.21 16
Memory Hierarchy 3.22 17
Creating Methods 3.3 17-18
File and Data Management 3.4 18-19
Section 4 Instrument Settings

Menu Options 4.1 20
Set Time & Date 4.2 20
User Preferences 4.3 20
Adjusting User PIN 4.4 21
Language 4.5 21
Method View Settings 4.6 21
Touch Screen Click 4.7 22
Adjusting Screen Contrast 4.8 22
Adjusting Date & Time 4.9 22
About 4.10 23
Form Feed… 4.11 23
Administrative Functions 4.12 23
Instrument ID 4.13 24
Create New User 4.14 24
Delete User 4.15 24-25
Delete All Users 4.16 25
Reset User PIN 4.17 25
Card Manager 4.18 26
Section 5 Photometrics Mode

Principles of Measurement 5.1 27
Mode Menus and Icons 5.2 27
Free Operation 5.3 28-30
Setting Method ID 28
Setting Wavelength 29
Setting Method Security 29
Auto Log Settings… 30
Logged In Measurement 5.4 31-36
Creating a New Method 32
Setting Batch ID 33
File & Data Management 35-36
Performing Measurements – all users 5.5 37-38
Zeroing the Instrument 3738
Measuring the Sample
Section 6 Spectrum Mode

Principles of Operation 6.1 39
Selecting Measurement Mode 41
Setting Wavelengths (Start & End) 41-42
Selecting Plot Interval 42
Setting Additional Set-up Parameters
using the Toolbar Icons 43-46
Setting Analysis Points 43
Setting Auto-Scale 44
Selecting Plot Colour 44
Selecting Axis Colour 45
Setting Batch ID 45
Setting Graph Data Points 45
Selecting Printer Media 46
Logged In Measurement 6.4
Setting Wavelengths (Start & End) 49
Selecting Plot Interval 50
Setting Additional Set-up Parameters
using the Toolbar Icons 51-54
Setting Analysis Points 51
Setting Batch ID 53
Performing Measurements – all users 6.5 57-61
Performing a Scan 57
Baseline 57
Scanning the Sample 57-58
Analysing the Scan 58-59
Area Under Curve 59
Co-ordinate Tagging 60
Auto Peak & Valley Tagging 60
Overlaying Scans 61
Section 7 Multi-Wavelength Mode

Principles of Operation 7.1 62
Selecting Sample Style 64
Setting Wavelengths 65
Calculations 65
Sum 65-66
Setting Batch ID 66
Selecting Sample Style 70
Setting Wavelengths 71
Calculations 71
Sum 71-72
Setting Batch ID 72
Performing Measurements – all users 7.5 76
Section 8 Kinetics Mode

Run Settings… 79-81
Setting Run Time 80
Setting Lag Time 80
Setting Start On Level 80
Setting Start Level 81
Setting Concentration Parameters 81-82
Selecting Resolution 82
Selecting Units 82
Setting Batch ID 84
Run Settings… 88-89
Setting Run Time 88
Setting Lag Time 89
Setting Start On Level 89
Setting Start Level 89
Setting Concentration Parameters 90-91
Selecting Units 91
Setting Batch ID 93
Section 9 Quantitation Mode
Setting method ID 99
Selecting Units 100
Replicates Setup… 101
Selecting Calibration Standards 102
Setting Curve Fit 102
Setting Auto Scale 103
Setting Batch ID 104
Selecting Units 108
Replicates Setup… 109
Selecting Calibration Standards 110
Setting Curve Fit 110
Setting Auto Scale 111
Setting Batch ID 112
Post Quantitation Tools 117
Section 10 Fitting Alternative Modules/Accessories

Integral Printer Unit 10.1 118
Cell Holder Modules 10.2 119
Section 11 Maintenance & Troubleshooting

General 11.1 120
Light Source Replacement 11.2 120-123
Tungsten lamp – Model 6700 11.21 120-121
Flash lamp module – Models 6705/6715 11.22 122-123
Glossary of Icons 124-126

Section 1
Introduction
1.1 Instrument Description
The 67 Series offer a range of unique features and functions to help in the management of methods
and data at both individual and multi-user levels.
These units have a specially designed user interface based on a high quality, colour TFT LCD with
touch screen technology and QWheel™ support to provide ease-of-use for all operations from set-up
to measurement and data handling.
Five main measuring modes are available: Photometrics, Spectrum, Kinetics, Quantitation and Multi-
Wavelength.
The 67 Series have dual memory options that enable you to store settings and data to either internal
memory or on a removable SD/Multi-Media memory card. By saving to the removable SD card all
settings, methods and results specific to the user can be retained by the individual, offering additional
security and allowing easy transfer of data to a PC for storage, manipulation or off-line review. The SD
card can also be used in any similar model, giving complete flexibility to use any available instrument.
A simple back-up procedure enables easy transfer of all information from the internal memory to the
removable media while quickly enabling a group of instruments to be cloned with identical settings.
It is possible for any user to perform simple measurements (Free Operation) at any time, however,
logging-in with your own PIN code will allow you to create methods, change settings or store results.
Each method can be designated as Public (for open user access), Read-Only (available for all users
as read-only information) or Personal (only accessed through your PIN code).
A number of sampling accessories are available as complete, easily interchangeable modules. These
include a Sipper Pump, Sipper/Peltier, Automatic Cell Changer, plus a comprehensive range of
passive cuvette and test tube holders, which can be fitted in the single cell holder accessory.
The PC Software supplied enables the transfer of data from the instrument or SD card to the PC with
rapid copy and paste into spreadsheets or other computer programmes.
Advanced software packages are also available for method creation, editing and memory
management.
(1)
1.2 Instrument Specifications
Technical Specification
6700 6705 6715
Light Source Tungsten Halogen Xenon Xenon

Spectral Bandwidth 4nm 4nm 1.5nm
Stray Light <0.1% at 340nm <0.05% at 220nm <0.05% at 220nm
Wavelength Range 320-1100nm 190-1100nm 190-1100nm
Common Specifications
Optics Sealed, MgF Coated, Split Beam

Wavelength Resolution 0.1nm
Wavelength Accuracy ±1.0nm
Wavelength Repeatability ±0.2nm
Photometric Ranges -0.300 to 3.000A & 0 to 199.9%T
Photometric Resolution 0.001A & 0.1%T
Photometric Accuracy ±0.005 at 1A
Photometric Stability <0.001A per hour
Quantitation Range -99999 to +99999
Number of Standards 20 with up to 5 replicates of each
Curve Fit Algorithms Linear, Quadratic and Cubic Functions
Multi-wavelength Data Points Up to 4 wavelengths
Calculations Difference and ratio
Kinetics Time Limits 0 to 9999 seconds
Kinetics Calibration Standard or factor
Scan Speed 1500nm/minute at 0.1nm data steps
Post Scan Analysis Peak/Valley pick, Peak Ratios, Area, Zoom, Wavelength
Table, Derivatives, Smoothing
Configuration Secure Multi-User and Free Access
Number of Users 10 + Supervisor
Number of Methods >1000 (on internal flash memory or removable media)
Results Storage >1000 (on internal flash memory or removable media)
Removable Media MM/SD Memory Card or SD/USB memory card
Interface USB, Centronics, Analogue
PC Software Supplied on CD-ROM with USB interface cable
Mains Supply 100 to 230V ac 50 or 60Hz
Sample Compartment 150mm (max. height) x 130mm (w) x 210mm (d)
Size 490x390x220mm
Weight 7.5Kg
Environmental Operating Conditions:
The 67 Series is designed to work safely under the following conditions:
Temperature 15 to 40ºC
Humidity 0 to 90%RH
(2)
Accessory Specifications
Peltier
Range Ambient +2°C to 60°C

Resolution 0.1°C
Regulation ±0.1°C
Accuracy ±0.5°C
Control System PID
Sipper Pump
Modes:
Continuous Aspiration Flow rate dependent on tube ID.
12ml/min with supplied tube
Timed aspiration sample/air-gap/wash
sample volumes 75µl min / 9.5ml max.
segment run time 48 secs max.
Memory Non-volatile
(3)
1.3 Good Practice Guidelines
1) For optimum performance all spectrophotometers should be sited in a clean, dry, dust free
atmosphere. When in use ambient temperature and light levels should remain as constant as
possible.
2) Adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP)
should be monitored with regular calibration checks and a suitable Quality Control (QC)
programme.
3) The sample chamber lid must be fully closed during measurement and before any readings
are recorded or printed.
4) The correct selection of sample containers is imperative for accurate and reproducible results:
a) Check that the material of the sample container is compatible with the wavelengths to be
used for measurement. In general glass can only be used down to 360nm or 320nm
depending on quality. Standard plastic cuvettes can be used down to 320nm. Special UV
versions can be used down to 260nm. Below this level quartz cuvettes must be used.
b) Plastic disposable cuvettes should be used ONCE only.
c) Glass cuvettes should be thoroughly cleaned after use. Discard when scratches become
evident in optical surfaces.
d) Care should be taken when selecting semi-micro or micro cuvettes. The cuvette window
on the inner chamber (the area filled with sample) must be wider than the aperture in the
sample holder or light will reach the detector without passing through the sample. In this
case, semi-micro or micro cuvettes with self-screening black surrounds must be used or
alternative holders for these cuvettes fitted.
e) Glass test tubes and other sample tubes should be used with care. Where possible,
matched tubes should be used and any index mark set to the correct position before
measurements are made.
f) Ensure any sample containers used are compatible with the constituents of both the
samples and standards they are to hold. Plastic cuvettes are not compatible with organic
solvents.
g) All sample containers must be handled with care; by the top and non-optical surfaces
only. Any finger marks evident must be removed using a suitable cleaning process.
h) Flow-through cuvettes must be selected with care and consideration for the sample type,
sample volume, pumping system, rinse, sample and waste handling to be used.
5) Samples and standards should not be stored in open cuvettes or sample containers as
evaporation will change the value and lead to staining of the walls which may be irreversible. If
stored in stoppered and sealed cuvettes, they should be filled with little or no air space and the
values regularly checked against a reference standard or quality control material.
6) Cold samples should be allowed to equilibrate to ambient temperature before measurement

(unless a suitable temperature controlled sample holder is in use). Temperature change
during measurement may cause air bubbles to form on the walls of the sample holder. This is
a common cause of drift during measurement.
7) In the preparation of samples and standards high grade borosilicate glass and AR grade
chemicals and reagents must be used. Good quality deionised water or other suitable solvent
must be used for dissolving or diluting samples, chemicals and reagents.
(4)
8) All measurements require calibration to a blank, for maximum accuracy this should be
prepared with care using the same deionised water or solvent used for dissolving or diluting
the sample. Where reagents are added to the sample to produce a colour proportional to its
concentration a ‘sample based’ blank should be used. In this case the blank should consist of
the sample plus any reagents or chemicals to be used, except those that produce the colour
to be measured.
9) Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-
linear response during sample concentration measurements. For all new methods a linear
range should be defined by the preparation of a calibration curve. The quantitation mode may
be used to construct such a curve against which sample results are automatically measured.
10) Cuvettes and sample holders must be filled to a minimum level which covers the light path.
(5)
Section 2
Getting Started
2.1 Unpacking Instructions
• Refer to label 1 on carton exterior and

ensure instrument type and
options/accessories supplied are correct.
Refer to Distributor in the event of any
discrepancy.
• Check each item as it is removed from

the packaging to ensure it is correct and
undamaged. Refer to Distributor if any
item is missing or damaged.
• Remove Documentation carton 2 .

Note that this contains the Instruction
Manual and other important documents,
which MUST be retained for future
reference. Other small items (CD ROM,
SD Card etc) will also be found inside
this carton.
• Please take some time to familiarise

yourself with the contents of the
Instruction Manual before using the
instrument for the first time.
• Remove the cardboard packing piece 3

and place to one side.
• Remove the two foam packing pieces 4

and place these to one side.
• Grasp the instrument 5 (which will be

sealed in a polythene bag) firmly at each
end, and lift out of the carton to place on
an adjacent flat, firm and clean surface.
The instrument weighs approximately 10
Kgs.
PLEASE TAKE CARE WHEN LIFTING.
• Remove the Accessory carton 6 . Note

that this contains the Power and USB
cables together with any sample cells or
other items that may have been ordered.
Refer to the Instruction Manual to ensure accessories are installed correctly before using the instrument
for the first time.
Disposal of Packaging
It is recommended that the Instrument packaging be retained for possible future long-term
storage or transportation. Please note that the Manufacturer or Distributor cannot be held
responsible for any damage incurred as a result of transportation of an inadequately packed
instrument.
(6)
If you wish to dispose of the instrument packaging, please do so in an environmentally
responsible manner. Please refer to the following guidelines: -
Cardboard packing items are made from paper sourced from recycled
fibres or managed forests and can be 100% recycled where appropriate
facilities exist. Ensure cartons are crushed or flattened before disposal.
Foam packing pieces are manufactured with a reprocessed polyethylene content and can
be easily recycled with other low-density polyethylene (LDPE) materials. Polyethylene
foam is manufactured by a CFC and HCFC free process and contains less than 100ppm
heavy metal content. The European Union Packaging Directive confirms that recovery by
means of waste-to-energy is a sensible waste management alternative. The Polyethylene
foam burns cleanly and contributes a high calorific value.
2.2 Installation
2.21 Location
In ideal circumstances the installation environment will be clean, dry and dust free with the instrument
protected from extreme variations in ambient lighting and temperature change. Ensure the unit is
positioned so that the mains on/off switch is accessible. If a safety problem should be encountered,
switch off at the mains socket and remove the plug from the supply.
Where conditions are less than ideal, maintenance and cleaning must be carried out regularly and
additional protection offered where possible.
The optional dust cover should be used to protect the instrument when not in use.
2.22 Supply Voltage

The 67 series spectrophotometers are powered by a universal switch-mode power supply that
operates from a 90-264Vac mains supply. The correct lead for your supply should be selected.
2.23 Mains Connections

The leads supplied have a moulded on plug. However, if this is removed for any reason the wires in
the mains lead are colour coded to conform to the internationally recognised standard such that:
UK CONNECTIONS US CONNECTIONS
BROWN LIVE BLACK LIVE
BLUE NEUTRAL WHITE NEUTRAL
GREEN/YELLOW EARTH GREEN EARTH
Safety
When disposing of any removed plug the connectors must be removed or made incapable of
insertion into a mains socket.
(7)
2.24 Touch Screen Functions
1. Standby – this key can be used to enter the standby mode during operation.
2. Instrument Settings – this key can be used to access instrument set up parameters including user
creation, PIN codes, language options, date and time settings,
administrative functions and instrument identification details.
3. Back key – this is used to return to a previous menu level.
4. Save key – pressing this key saves methods and/or data. If an external card is not fitted then
methods/data will automatically be saved to internal memory. If an SD card is inserted
or removed during operation relevant messages and options are given to select the
desired media.
5. Toolbar key – this key is only functional on the completion of measurements and gives access to
the available data manipulation options. The function of this key is context sensitive,
enabling different sets of tools depending on the mode of operation in use.
6. Printer key – this key will initiate a print out to either the internal or external printer unit via the
selected settings options in each mode of operation.
(8)
2.25 Toolbar Icons
The spectrophotometer can be used for making measurements at any time without the need for
logging in; but many benefits will be lost and results cannot be saved, only printed.
The Method Screen options will only be displayed if the logs in. If the user is not logged in the
instrument will automatically display the main measurement screen, with settings at their last used
levels.
Method Screens
Methods are stored sequentially by measurement mode. Once the first page is full (8 methods for the
selected mode) cursor arrows are displayed enabling the user to browse to subsequent or previous
pages of 8 methods.
Alternatively, pressing one of the alphanumeric keys along the bottom of the screen will display all
available methods with the initial character that is highlighted.
Note: Repeated pressing of a key sequentially highlights the characters between those displayed.
Create a New Method
For the following functions first touch a method or result to select it.
Open the Selected Method
Erase the Selected Method
Browse Results – linked to the selected method
Open specific results in the selected batch
(9)
Photometrics Mode
Mode settings – method name, wavelength, method security (if logged in)
Accessory options – varies with type of accessory module fitted
Allows selection of internal or external printer
Allows set-up of batch ID and the Auto Save options
Press to accept settings entered
Spectrum Mode
Mode settings – method name, measurement mode, wavelength range, plot interval, method
security (if logged in)
Analysis Points – up to 30 wavelengths at which absorbance will be reported
Measurement display – auto scaling, axis setting, colour selection
Allows selection of internal or external printer, graph details, batch ID and enables or
disables the Auto Save feature
(10)
Multi-Wavelength Mode
Mode settings – method name, measurement type, dwell time, reading type, sample style,
method security (if logged in)
Setting wavelengths – allows from 2 to 4 wavelengths to be set
Calculations - allows the selection of calculations and constants
Allows selection of internal or external printer, graph details, batch ID and the Auto
Save options
(11)
Kinetics Mode
Mode settings - method name, wavelength, measurement mode, run settings, method security
(if logged in)
Calibration – allows setting of Concentration cal standard, factor, resolution and units of
measurement
Measurement display – allows auto scaling, axis setting, colour selection
Save options
(12)
Quantitation Mode
Mode settings – method name, measurement mode, wavelength, resolution, units of

measurement, replicates set up, method security (if logged in)
Calibration – allows the number and levels of standards to be set
Measurement display - – allows auto scaling, axis setting, colour selection
Save options
Status Bar
To view the set parameters in any of the measurement modes press the status bar once and a drop
down menu will appear. Pressing this bar again will return it to its original status.
(13)
2.26 Rear Panel Layout
Power Switch - On/Off switch for the unit.
Power In Socket - IEC type connection socket for the mains supply cable.
Power Out Socket - IEC type connection socket for auxiliary equipment.
Printer Socket - 25 way Centronics parallel output socket compatible with

Postscript printers.
Analogue Output - 2 x 4mm pin sockets for analogue devices.
USB Socket - type B connector for communication to PC.
SD Card Socket - will accept 128MB to 2GB SD or Multi-media memory cards.
(14)
2.27 Power on and Self-Tests
Connect the mains supply cable to the rear panel mains input socket and plug the other end into a
suitable mains supply socket.
Lift the sample chamber lid on the instrument and ensure that there is no sample or other item present
in the sample holder, close the lid.
Switch on the supply socket, then the instrument, using the Power switch located on the rear panel.
The instrument will then perform the power on self-test protocol. The power on and self-test screen will
be shown:
System test… Checks internal connections / Checks internal and external memory
status
Accessory test… Checks for fitted ‘active’ accessories / Verifies communication and
response
Dark test… Checks detector and light seal on sample chamber
Wavelength calibration… Performs a wavelength calibration
followed by the Main Menu Screen:
This screen gives you access to the five operating modes that can be used directly for making
measurements without logging in. It should be noted that if the user is not logged in results can only
be viewed and printed but not stored. In addition, methods cannot be created or retrieved.
(15)
Section 3
Systems of Operation
3.1 Multi-User and Free Operation
The 67 Series spectrophotometers focus on secure, multi-user operation, but to ensure that anyone
can acquire quality results in an emergency or when otherwise required (stat or free operation), they
may also be used without the operator having to log in. Such free operation is restricted to making
measurements with no access to methods while results will only be displayed or printed.
Secure, multi-user operation requires each designated user to enter a PIN code to access methods
and results. When creating methods a user can opt to share them with other users, as Public (can be
modified) or Read-Only (can be used but not modified), or to keep them Personal. Up to 10 users
and a Supervisor with administrative rights can be set up on each instrument but by using the
removable media (see below) an almost infinite number of users can be securely accommodated.
3.2 Memory Structure
The 67 Series spectrophotometers have a dual memory structure based on removable media and
fixed internal memory. The capacity of the internal memory can be selected when an instrument is
purchased; further up-grade to this internal memory is only possible by a trained and qualified Service
Engineer. The removable media is based on SD or Multi-Media memory cards with a wide range of
capacities available as well as dual function SD/USB cards that can be used to directly transfer data
from the SD card to the USB port of a personal computer.
For both internal memory and removable media the following table of memory capacity and data
storage can be used as a guide to the files that can be stored:
Memory Size Methods Results Total Files

256 MB 1000 1000 2000
512 MB 2000 2000 4000
1 GB 4000 4000 8000
2 GB 8000 8000 16000
The following table gives details of the removable media available (subject to change by
manufacturers range consolidation):
Card Capacity SD Card Part Number SD/USB Card Part Number

256 MB 019 130 Not available in this size
512 MB 019 131 019 134
1 GB 019 132 019 135
2 GB 019 133 019 136
3.21 Memory Selection
If a removable media memory card is fitted when the power is turned on this becomes the default
memory location and any methods and results saved will be sent to this card. Should the card be
removed during use a warning message will be displayed and the internal memory will then be set as
the default location.
If no removable media is fitted when the power is turned on the internal memory becomes the default
memory location Should a removable media memory card be fitted during use a message is displayed
giving the option to switch the default location to the removable media memory card.
(16)
3.22 Memory Hierarchy
The top level of the memory hierarchy is the user, defined by a user name and recognised by their
unique PIN code. In general it is considered that this will be an individual but in practice this can be
made more flexible by considering the user as a group sharing the same PIN code. For instance
different departments or shifts could be identified by their own PIN codes.
User defined by
PIN Code
Read-Only Public
Methods Methods
Methods
Created by User
Results appended to
user accessible methods
Different types of tests could also be separated by PIN codes with only users trained in carrying out
specific tests given the relevant PIN codes to access the tests for which they have been trained. In
education a PIN code for each semester or part of the curriculum would restrict students or year
groups access to only the current work, protecting that to be used in the future. The implementation
and benefits of this function are only limited by the imagination.
Below the user level are the Methods created by that user or for that group, and those with Public or
Read-Only status that all users can access, below each method are the results stored by the user for
that method.
3.3 Creating Methods
The ability to create and re-call methods is only available to logged in users.
To log in touch the Log In… key on the bottom right-hand side of the Main Menu screen that is
displayed after completion of the start-up tests.
A table of users is then displayed, touching your name (department, user group or other identifier)
then brings up a numeric entry screen for inputting the four digits of the relevant PIN code.
For first time use the only user in the table will be the Supervisor with a default PIN Code of 1234
(once logged in this can subsequently be changed to a number combination of the Supervisor’s
choice).
By pressing the Settings key on the display surround and selecting the Administrative Functions
option the Supervisor can create new users. On creation a new user has a default PIN Code of 0000
that, like the Supervisor’s, can be changed by selecting User Preferences, then Adjust PIN Code.
NOTE: Once the Supervisor’s PIN CODE is changed it must be kept safe as there is no other way of
accessing this function – however the Supervisor does have the right to re-set other users’ PIN Codes
back to the default.
(17)
For further information on these functions see the Section on Instrument Settings and
Administrative Functions.
When successfully logged in the user is returned to the main menu screen from which the required
mode of measurement can be selected.
The Browse screen is then displayed from which an existing method can be re-called using the
alphanumeric search keys along the bottom menu bar. If insufficient methods have been created to fill
the first page all available methods will be shown.
3.4 File & Data Management
Save Tools Print Settings Erase Search Open Save

Results Results
Saving Methods
Having entered all your required settings on the tabbed pages the method can be saved by simply
pressing the Save key on the display surround. If you do not save at this point but continue to make
measurements using the method, it will automatically be saved when you save the first result.
If you continue without saving a result you will be prompted to save the method as you exit the
operating mode or return to the settings options.
Sharing Methods
Methods can be shared with other users by setting the security level at either Read-Only where other
users can use but not modify the method or, Public where they can use and modify the method. Other
users must then ensure that under Method View Settings (Settings/User Preferences/Method View
Settings) they have enabled Public and/or Read-Only methods or turned All Methods on.
Recalling Methods
Following selection of the operating mode from the main menu the method browse screen is
displayed. This will show all methods that the current user has access to, based on their selections in
the Method View Settings (refer to Sharing Methods). If the first page is full, cursor arrows will be
displayed to enable navigation to and from additional pages.
Alternatively, selection may be made using the alphanumeric menu bar at the bottom of the screen.
Repeated pressing of each character set will display the full alphanumeric range and the screen will
show all methods starting with the highlighted character.
Touch the required method when it is displayed on the screen to highlight it, touch the Open File icon
to display the main measurement screen for that method.
Editing Methods
Use the Recalling Methods procedure to recall the required method. With the measurement screen
displayed touch the Settings option. Adjust the settings as required and touch the Enter icon on
completion. The modified method can then be saved by pressing the Save key on the display
surround. If you do not save at this point but continue to make measurements using the method, it will
automatically be saved when you save the first result. If you continue without saving a result you will
be prompted to save the method as you exit the operating mode or return to the settings options.
Note: If the Method name was not changed during editing it will be saved with the same name but with
a new date and time to ensure traceability. If the old method is no longer required it should be deleted
as detailed in Deleting Methods.
(18)
Deleting Methods
To delete methods highlight the required method in the Browse screen as described in Recalling
Methods and then touch the Erase icon.
A warning message will be displayed to ensure this action is required. On confirmation the selected
file will be deleted.
If the current user does not have the required privileges to delete the selected message then an
information message will be displayed advising that the method cannot be deleted.
Privileges required for deleting designated methods:

Public Methods – only the Supervisor can delete these.
Read-Only Methods – only the Supervisor and Originator can delete these.
Personal Methods – Only the Originator can delete these methods. (The Supervisor can delete these
by re-setting the Originator’s PIN code and then logging in as the Originator).
Saving Results
After completion of a measurement the result can be saved by simply pressing the Save key on the
display surround. The result is saved under the method that created it, with the entered Batch ID and
an incremental number along with the time and date of the measurement. Results can also be saved
as part of the Auto Log function, which will vary depending on the type of sampling accessory fitted.
Printing Results
After completion of a measurement the result can be printed, by simply pressing the Print key on the
display surround. The result will be printed to either the internal or external printer, as selected by the
user in the Printer Settings option.
The first result of any new batch is preceded by a print header, which gives details of the method
settings and Batch ID. Results can also be printed as part of the Auto Log function, which will vary
depending on the type of sampling accessory fitted.
Recalling Results
Stored results are always directly linked to the method that created them. To access results first recall
the method as described in Recalling Methods (page xx). With the method highlighted touch the
Search Results icon. This will open a screen detailing all results available to the current user. Touch
the required result or batch (depending on the mode) and then the Open Specific Result icon. This
will display the results on the screen. The Tools option can then be used to work on these results
(depending on mode). It is also possible to print the result by simply pressing the Print key on the
display surround. Options to print to the Internal or External printer or to the Data Card will be
displayed. Printing to the Data Card will save an EPS (Encapsulated Postscript) file to the Data Card.
This is in printer ready format and can be viewed and printed in a number of software packages,
including Adobe PhotoShop®, Corel Draw®, Ghostscript® and many other file management systems.
Sharing Results
Results attached to Personal Methods cannot be accessed by any other user.

Results attached to Read-Only and Public Methods can be accessed by all users, based on their
current Method View Settings.
Deleting Results
Results can only be deleted by the Originator or the Supervisor through Administrative Functions.
Recall the required result without opening it. Highlight the result (or batch of results) and then press
the Erase icon. A warning message will be displayed to ensure this action was intended. On
confirmation the results will be permanently deleted.
(19)
Section 4
Instrument Settings
4.1 Menu Options
A number of settings can be stored for each signed in user including language, method view settings,
display brightness and time/date display choices so that these do not have to be reset each time a
new user logs in. These can be found under User Preferences.
– When switching on the product for the first time, or if the previously entered settings
need to be checked, this key should be pressed to give access to the following display:
4.2 Set Time & Date
To set the correct date and time select the Set Time & Date key. The time and date can be reset
using the up and down arrows. To accept the new settings press the Enter key. Pressing the Cancel
key will return you to the main menu without accepting any alterations to the current settings.
4.3 User Preferences
Selecting User Preferences gives access to the following options:
These settings are saved for each user and the individual’s selections are retained when that user
logs in.
(20)
4.4 Adjust User PIN
To alter your User PIN select the Adjust User PIN key. Enter your new 4 digit code using the numeric
keypad as shown below. The Del key allows individual digits to be changed. The Cancel key will
return you to the previous menu without altering the original PIN code. The Clear key will clear all
digits entered from the screen. The Enter key accepts the new code and returns you to the previous
menu.
4.5 Language
The preferred language can be selected by pressing the Language: English key. Each time this key
is pressed the instrument will update to show the next available language in the following sequence:
English, Francais, Deutsche, Italiano, Espanol.
Once the preferred language option is selected, pressing the Back key on the main panel twice will
return you to the Main Menu options.
4.6 Method View Settings
To simplify operation it is possible to restrict the methods displayed by their allocated category.
Selecting Method View Settings enables My Methods (Personal) to be hidden (Off) or displayed
(On). Similarly, Read Only Methods and Public Methods can be hidden or displayed. To simply
display all method categories toggle All Methods to the On status.
(21)
4.7 Touch Screen Click
The touch screen can be operated with or without an audible click each time a key is pressed. The
click can be toggled on or off via the Touch Screen Audible Click: Off key.
4.8 Brightness
Selecting the Brightness key and then using the left and right arrows on the display shown below can
adjust the brightness of the display. Pressing the Save key will update the display setting and return
the instrument to the previous display.
4.9 Adjust Date & Time
Selecting the Date & Time Preferences key allows the user to display either the time or date. In
addition the date format can be set to either DD/MM/YY or MM/DD/YY. Once the preferred option is
selected, pressing the Back key on the main panel once will return you to the previous menu.
Pressing the Back key twice returns the instrument to the Main Menu.
(22)
4.10 About
Selecting the About key provides information relating to instrument ID and signed in user details.
Pressing the OK key returns the instrument to the previous menu.
4.11 Form Feed
Selecting the Form Feed key will feed additional paper through the mechanism of the connected
printer to separate results or enable a clean area for tearing off. If no internal printer is fitted this option
defaults to external printer.
4.12 Administration Functions
This function is only available to the supervisor. It gives access to all user related information. It allows
the Supervisor to create new users, delete an individual or all users and reset user PIN codes. If the
Supervisor is not logged in this option will be greyed out and unavailable.
(23)
4.13 Instrument ID
Selecting the Instrument Id: 67XXCVS key allows the supervisor to alter the instrument identification
code. This is entered by use of the alphanumeric keypad. The Cancel key will return you to the
previous menu without altering the original ID. The Del key allows individual letters or digits to be
removed. The Clear key will clear all digits entered from the screen. The Enter key accepts the new
ID and returns you to the previous menu. For laboratories with more than one 67 series
spectrophotometer it is useful to change this ID to the serial number, department or other unique
identifier for easy recognition of different instruments.
4.14 Create New User
Selecting the Create New User key allows the supervisor to enter an additional user on to the
instrument using the alphanumeric keypad as shown. The Clear key will clear all information entered
from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will
return you to the previous menu without adding a new user. The Enter key accepts the new ID and
returns you to the previous menu.
4.15 Delete User
Selecting the Delete User option allows the supervisor to delete a specific user from the instrument.
When the user name is selected from the list the following warning message is displayed:
6715 CVS
This user will be permanently

deleted from the instrument.
Are you sure you wish to
continue?
Yes Cancel
(24)
If the Yes key is pressed the instrument will update and the user will be removed from the instrument.
If the Cancel key is pressed the user will not be deleted from the instrument.
4.16 Delete All Users
Selecting this option allows the supervisor to delete all current users (except the supervisor) from the
instrument. The following warning message is displayed:
6715 CVS
All users will be permanently

deleted from the instrument.
Are you sure you wish to
continue?
Yes Cancel
If the Yes key is pressed the instrument will update and all users will be removed from the instrument.
If the Cancel key is pressed all users will remain on the instrument.
4.17 Reset User PIN
This option allows the supervisor to reset an individual users PIN code.
Select the Reset User PIN option from the screen. The list of current users will then be displayed on
screen. Select the user. The warning message: ‘Are you sure you wish to continue’ will be
displayed with the options to accept or cancel.
If the Yes key is pressed the user PIN will be reset to the default value and the supervisor has access
to all information relating to that user.
If the Cancel key is pressed no changes will be made to the user codes and the instrument will return
to the Main Menu.
(25)
4.18 Card Manager
This option is available only to the supervisor and allows transfer of data, methods and user
preferences to and from the instrument memory. This enables backup of information stored in the
instrument memory and on any removable media used. Both memory locations can also be formatted.
WARNING – this will permanently delete all information stored in the location being formatted.
(26)
Section 5
Photometrics Mode
5.1 Principles of Measurement

The simplest mode of the spectrophotometer is Photometrics. A measurement is made of either the
absorbance or transmittance of a sample. The measurement is at a single wavelength, at one point in
time, with no additional calculations.
Select Photometrics from the Main Menu:
If the user is not logged in (Free Operation) the main measurement screen will be displayed (refer
Section 5.3).
5.2 Mode Menus and Icons
Mode settings – method ID, wavelength, method security (if logged in)
Allows selection of internal or external printer
Allows set-up of batch ID and enables or disables the Auto Save feature
(27)
5.3 Free Operation
Settings
If the user is not logged in then the main measurement screen will automatically be displayed when
the Photometrics Mode is selected from the Main Menu:
To enter the appropriate wavelength for the sample(s) to be tested select the Settings key and the
instrument will display the following screen:
Setting Method ID
To allocate a Method name select the Method key and enter the preferred name using the
alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key
allows individual letters or digits to be removed. The Cancel key will return you to the previous menu
and the method name will remain as Default Method. The Enter key accepts the new method ID and
Note: The new Method ID can only be used to identify the method on a printout to the internal or
external printer module. The generic user has no additional facility to store or recall results or
methods.
(28)
Setting Wavelength
Select the Wavelength key and enter the wavelength using the numeric keypad. The Clear key will
clear all information entered from the screen. The Del key allows individual digits to be removed. The
Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key
accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order
selecting filters may be heard switching in or out). Press the Enter key to confirm. The display will
momentarily flash the message ‘Going to Wavelength’ and the display will update to show the new
wavelength.
Using the Back arrow to escape settings will display: 6715 CVS
Continue? , if so you will

If Yes is selected all set information will be lost and lose any changes you have
the instrument will return to the main measurement made.
screen. If No is selected the instrument returns the
Settings screen as shown. Yes Cancel
To accept all entered information press the Enter key to confirm. The instrument will show the main
measurement screen with the updated information.
Batch ID and Auto Logging can also be entered via the Save icon.
Setting Method Security
If the user is not logged in (i.e. Free Operation), this feature is non functional.
Setting Batch ID
Select the Batch ID key and enter the Batch code using the alphanumeric keypad. Press Enter to
accept or Cancel to remain as a default.
(29)
Auto Log Settings…
Selecting Auto Log Settings… opens a dedicated sub-menu.
This option can be toggled between On/Off. When the Auto Log option is selected to On the user can
set the following options:
the timed interval between 10 and 9999

seconds using the numeric keypad;
the printer destination (toggles

between internal or external);
the number of repetitions from 2 to 9999

using the numeric keypad.
Pressing the Enter key accepts the entered information and returns the instrument to the main
measurement screen.
To view the set parameters press the status bar once and a drop down menu will appear giving this
detail. Pressing the bar again will return it to its original position.
(30)
5.4 Logged In Measurement
PIN Codes – each user is allocated a 4 digit PIN code that is required when logging in.
Select Log In… from the Main Menu and a list of users will be shown. Select the appropriate user
name and a numeric keypad will be displayed.
Enter your 4 digit PIN code and press the Enter key to confirm.
Once logged in method screen options will be displayed:
pages of 8 methods.
Create a New Method
(31)
Creating a New Method
Settings
To enter the Method ID, appropriate wavelength for the sample(s) to be tested and the level of Method
Security required for the method being created select the New File icon
Setting Method ID
Setting Wavelength
selecting filters may be heard switching in or out). Press the green tick to confirm. The display will
momentarily flash the message ‘Going to Wavelength’ and the display will update to show the new
wavelength.
If Yes is selected all set information will be lost and Continue? , if so you will
lose any changes you have
the instrument will return to the main measurement made.
(32)
The 67 Series spectrophotometers can support up to 10 individual users plus one Supervisor who has
full administrative rights.
Logged in users can create methods with three levels of security options:
Personal – these methods are only accessible by the originator.
Public – these methods are available for use and modification by any logged in user.
Read-Only – these methods can be accessed by all logged in users, but can only be modified by the
originator.
The preferred level of protection can be achieved by selecting the Method Security key that toggles
between Personal, Public and Read-Only options.
To accept the selected options press the Enter key to confirm. The instrument will show the main
Setting Batch ID
Select the Batch ID key and enter the Batch code using the alphanumeric keypad. Press Enter to
(33)
Selecting the Auto Log Settings… option opens a dedicated sub-menu.

This option can be toggled between On/Off. Switching the Auto Log to On allows timed intervals to be
set internal or external printer selection and the number of repetitions required.
This option can be toggled between On/Off. When the Auto Log option is selected to On the user can
set:
the timed interval between 10 and 9999 seconds

using the numeric keypad;
the printer destination (toggles between

internal or external);
the number of repetitions from 2 to 9999 using

the numeric keypad.
measurement screen.
To view the set parameters press the status bar once and a drop down menu will appear giving this
detail. Pressing the bar again will return it to its original position.
(34)
File & Data Management

Results Results
Saving Methods
Sharing Methods
Recalling Methods
displayed to enable navigation to and from additional pages. Alternatively, selection may be made
using the alphanumeric menu bar at the bottom of the screen.
Editing Methods
Deleting Methods
Methods and then touch the Erase icon. A warning message will be displayed to ensure this action is
required. On confirmation the selected file will be deleted.
(35)
Saving Results
Printing Results
Recalling Results
Sharing Results

Deleting Results
(36)
5.5 Performing Measurements – all users
Once the required settings have been confirmed the main Photometrics measurement screen will be
shown. This screen shows the absorbance value, followed by the Transmittance value and then the
selected wavelength. At the top of the screen Method ID and Batch no., time or date is displayed.
Zeroing the Instrument
Insert a cuvette containing a blank solution into the sample holder and close the sample chamber lid.
(Test tubes or other sample containers may be used depending on the sample holder accessory
fitted). Press the Zero key. The messages ‘Calibrating Dark Level… and Calibrating Light Level... ’
are displayed momentarily. Once the measurement is completed the screen will update to show:
Note: In general the blank solution should contain everything that is in the sample except the colour-
producing component. For specific information reference should be made to the procedure or
application being followed. For enhanced reproducibility matched cuvettes should be used.
Attempting to initiate a Read before zeroing

6715 CVS
the instrument displays the message:
Please perform a zero
Pressing OK returns the instrument to absorbance calibration
the measurement screen. first. Press OK to continue.
OK
(37)
Measuring the Sample
Remove the cuvette containing the blank solution and insert the first sample into the sample holder
and close the sample chamber lid. Press the Read key. The screen will momentarily display ‘Taking
reading. Please wait…’ Once the measurement is completed the screen will update to show the
measured value.
Additional individual samples can be measured by inserting them in the single sample holder and
pressing Read. Alternatively, multiple samples can be measured with the optional 8-cell changer or
sequentially with the sipper pump accessory.
(38)
Section 6
Spectrum Mode
By examining the absorbance or transmittance of the sample over a wavelength range we can partially
characterise the sample. Readings of the sample over a range of wavelengths are made and a graph
is plotted of the absorbance or transmittance at each wavelength. As well as observing good practice
(see Good Practice Guidelines) it must be ensured that the sample is stable for the period of the scan
(maximum 70 seconds).
Select the Spectrum Mode from the Main Menu options.
The method screen options will only be displayed if the user logs in. If the user is not logged in the
instrument will automatically display the main instrument screen, with settings at their last used levels.
Mode settings – method name, measurement mode, wavelength range, plot interval, method
Analysis Points – up to 30 wavelengths at which absorbance will be reported
Measurement display – auto scaling, axis setting, colour selection
Allows selection of internal or external printer, graph details, batch ID and enables or
disables the Auto Save feature
(39)
6.3 Free Operation
Settings
the Spectrum mode is selected from the Main Menu:
To enter the required parameters for the sample(s) under test, select the Settings key and the
Setting Method ID
external printer module. The generic user has no additional facility to store or recall results or
methods.
(40)
Selecting Measurement Mode
The Measurement Mode key toggles between Absorbance and Transmittance.
Setting Wavelengths
It should be noted that if the start or end wavelength is adjusted so that they are closer than twice the
plot interval then the previously set wavelength will be automatically reset to give a minimum of two
measurement points.
Setting Start Wavelength
Select the Start Wavelength key and enter the wavelength using the numeric keypad. The Clear key
will clear all information entered from the screen. The Del key allows individual digits to be removed.
The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key
show the entered wavelength.
(41)
Setting End Wavelength
Select the End Wavelength key and enter the wavelength using the numeric keypad. The Clear key
Selecting Plot Interval
Selecting the Plot Interval key allows the user to toggle between 1.0nm, 5.0nm, 0.1nm and 0.5nm.
If Yes is selected all set information will be lost and Continue? , if so you will
the instrument will return to the main measurement lose any changes you have
made.
To accept the entered information at any time press the Enter key to confirm. The instrument will
show the main measurement screen with the updated information.
To view the set parameters press the status bar once and a drop down menu will appear. Pressing
this bar again will return it to its original status.
(42)
This option is only available to logged in users.
Setting Additional Set-up Parameters using the Toolbar Icons
Setting Analysis Points
Up to 30 analysis points can be set be selecting the Analysis Points keys and entering the required
wavelength value via the numeric keypad.
It should be noted that a warning message will be displayed if the analysis point(s) set are outside the
spectrum scan range and a warning symbol will be shown next to the entered value.
The Clear Analysis Points option will clear all previously set analysis points.
If an attempt is made to set an analysis point outside the start and end wavelength values the error
message ‘Wavelength for analysis out of spectrum scan range’ will be displayed momentarily and
a triangular symbol will be shown to the side of the incorrect value. This value can be deleted or edited
by selecting the analysis point (in this example 1) and entering a new value within the specified limits.
The analysis points will appear on tags at the relevant point on the spectrum display. They will also be
printed with any printout to the internal or external printer if the Print Data Points option has been
selected.
(43)
Setting Auto-Scale
The Auto-Scale key toggles between On and Off. With the Auto-Scale function On the instrument
will automatically set the Y-axis maxima to a level that will fit the largest peak and lowest valley in the
display area. This is the best option to choose if the user is uncertain of the limits of the scan.
When set to On the manual settings for Y-axis maximum and minimum are non-functional. These
settings can be changed with Post Scan analysis tools to enable alternative views and printouts after
the scan has been completed.
With the Auto-Scale function set to Off the Y-Axis Minimum enables the lowest displayed level for
the Y-axis to be manually set. Press the Y-Axis Minimum key and a numeric keypad will be displayed
for entry of this value. This value can be between –0.300 and 2.999.
The Y-Axis Maximum enables the highest displayed level for the Y-axis to be manually set. Press the
Y-Axis Maximum key and a numeric keypad will be displayed for entry of this value. This value can
be between 3.000 and –0.299.
Selecting Plot Colour
Pressing the Plot Colour key displays a colour selection screen. Touch any one of the eight
colours to select the preferred plot colour. Once selected, the instrument will update to show the
selected colour.
(44)
Selecting Axis Colour
Pressing the Axis Colour key displays a colour selection screen. Touch any one of the eight colours
to select the preferred colour. Once selected, the instrument will update to show the selected colour.
Setting Batch ID
Select the Batch ID key and enter the batch code using the alphanumeric keypad. Press
Enter to accept or Cancel to remain as a default.
Setting Graph Data Points
The option to print graphs on a full page is only available if the External printer is selected. If available
and set to On the scan will be printed on one page (typically A4 landscape format) with the data on
following pages.
If available and set to Off the scan will be printed with the data below it (typically top half A4 portrait).
The Print Graph Data Points key toggles between On and Off.
With this key set to On all the data points that make up the graph will be printed in a table following the
scan. For a long scan at 0.1nm resolution this can result in many pages of data. It is recommended
that careful consideration should be given to this and that this feature should be set to the Off position
in this instance.
(45)
Selecting Printer Media
The Printer Media options toggle between Internal and External Printers.
Selecting Auto Log Settings… opens a dedicated sub-menu. This facility can be toggled between Off
and On. When the Auto Log option is selected to On the user can set the following options:
the timed interval between 10 and 9999 seconds using the numeric keypad;
the printer destination (toggles between internal or external);
the number of repetitions from 2 to 9999 using the numeric keypad.
measurement screen.
(46)
Once signed in method screen options will be displayed:
pages of 8 methods.
Create a New Method
(47)
To enter the Method ID, appropriate measurement parameters and the level of Method Security
required for the method being created select the New File icon.
Setting Method ID
(48)
Setting Wavelengths
It should be noted that if the start or end wavelength is adjusted so that they are closer than twice the
plot interval then the previously set wavelength will be automatically reset to give a minimum of two
measurement points.
Setting Start Wavelength
Select the Start Wavelength key and enter the wavelength using the numeric keypad. The Clear key
Setting End Wavelength
Select the End Wavelength key and enter the wavelength using the numeric keypad. The Clear key
(49)
Selecting Plot Interval
Selecting the Plot Interval key allows the user to toggle between 1.0nm, 5.0nm, 0.1nm and 0.5nm.
Using the Back arrow to escape settings will display:

6715 CVS
If Yes is selected all set information will be lost and
Continue? , if so you will
the instrument will return to the main measurement lose any changes you have
screen. If No is selected the instrument returns to the made.
Settings screen as shown.
Yes Cancel
originator.
To accept the selected options press the Enter key to confirm. The instrument will show the main
(50)
Setting Additional Set-up Parameters using the Toolbar Icons
Setting Analysis Points
Up to 30 analysis points can be set be selecting the Analysis Points keys and entering the required
wavelength value via the numeric keypad.
The Clear Analysis Points option will clear all previously set analysis points.
If an attempt is made to set an analysis point outside the start and end wavelength values the error
message ‘Wavelength for analysis out of spectrum scan range’ will be displayed momentarily and
a triangular symbol will be shown to the side of the incorrect value. This value can be deleted or edited
by selecting the analysis point (in this example 1) and entering a new value within the specified limits.
The analysis points will appear on tags at the relevant point on the spectrum display. They will also be
printed with any printout to the internal or external printer if the Print Data Points option has been
selected.
(51)
Setting Auto-Scale
will automatically set the Y-axis maxima to a level that will fit the largest peak and lowest valley in the
display area. This is the best option to choose if the user is uncertain of the limits of the scan.
settings can be changed with Post Scan analysis tools to enable alternative views and printouts after
the scan has been completed.
for entry of this value. This value can be set between –0.300 and 2.999.
be set between 3.000 and –0.299.
Pressing the Plot Colour key displays a colour selection screen. Touch any one of the eight colours to
select the preferred plot colour. Once selected, the instrument will update to show the selected colour.
(52)
Setting Batch ID
Select the Batch ID key and enter the batch code using the alphanumeric keypad. Press Enter to
and set to On the scan will be printed on one page (typically A4 landscape format) with the data on
following pages.
If available and set to Off the scan will be printed with the data below it (typically top half A4 portrait).
scan. For a long scan at 0.1nm resolution this can result in many pages of data. It is recommended
that careful consideration should be given to this and that this feature should be set to the Off position
in this instance.
(53)
Selecting Auto Log Settings… opens a dedicated sub-menu. This facility can be toggled
between Off and On. When the Auto Log option is selected to On the user can set the following
options:

measurement screen.
(54)

Results Results
Saving Methods
Sharing Methods
Recalling Methods
Editing Methods
Deleting Methods
(55)
Saving Results
Printing Results
Recalling Results
Sharing Results

Deleting Results
(56)
Performing a Scan
Once the required settings have been confirmed the main Spectrum measurement screen will be
shown with the Scan key inactive (blanked out):
Performing a Baseline
Insert a cuvette containing a blank solution into the sample holder and close the sample chamber lid.
(Test tubes or other sample containers may be used depending on the sample holder accessory
fitted). Press the Baseline key. The messages ‘Performing baseline scan. Please wait…’ are
displayed momentarily. Once the baseline measurement and storage is completed the screen will
update to show:
Note: In general the blank solution should contain everything that is in the sample except the colour-
producing component. For specific information reference should be made to the procedure or
application being followed. For enhanced reproducibility matched cuvettes should be used.
Scanning the Sample
Once the baseline has been performed the Scan key will be active. Remove the cuvette containing
the blank solution and insert the sample into the sample holder and close the sample chamber lid.
Press the Scan key, if Auto Scale has been selected once the scan is completed, the screen will
blank and the scan will be re-drawn to the optimum scaling.
(57)
Additional individual samples can be measured by inserting them in the single sample holder and
pressing Read. Alternatively, multiple samples can be measured with the optional 8-cell changer or
sequentially with the sipper pump accessory.
Analysing the Scan
Selecting the icon gives access to post scan analysis options, as detailed below:
Note: All tools functions are available for use on recalled results in exactly the same manner as
described hereafter.
Toolbar Icons
Display Settings
Toggles Auto Scale (Y Axis) On or Off. If Off Y axis

defaults to manual settings entered in the method.
Cycles round Plot Interval settings of 0.1, 0.5, 1.0

and 5.0nm
Zoom
Activates the zoom area select cursors that can be

positioned using the QWheel™ over the area
required. Repeated presses increase the zoom up to 5
times.
Returns the zoom to the next lower level. At x1 this

icon is disabled.
(58)
Accepts and re-scales the selected area to the current
zoom setting. Pressing the Back key or the Exit Door
once maintains the zoom display and gives access to
the tools for manipulation and analysis of the zoomed
portion. A further press of the Back key returns the
original scan.
Area Under Curve
Toolbar Icons
Area Under Curve Cursor Select Calculator
Pressing the Area Under Curve icon displays the area select cursors. This can be carried out on
zoomed or recalled spectra as well as the current result. Initially both cursors are active.
Use the QWheel™ to position the right hand cursor at the right hand side of the curve to be analysed.
Press the Cursor Select icon to fix this position, enabling the QWheel™ to move the left hand cursor
to the other side of the required curve.
A further press of the Cursor Select icon fixes this position but frees the right hand cursor for further
fine adjustment. (A further press enables both cursors again and so on round the cycle).
When the required area has been defined pressing the Calculator icon displays the area under the
curve, both as the displayed tangent (Tang.) to the valleys, and as a continuum to the X-axis (Base).
The wavelengths at the start and end of the curve are also displayed.
(59)
Co-ordinate Tagging
Toolbar Icons
Co-ordinate Tagging Tag Delete Tag
Peaks, valleys or any other points of interest can be selected (on the current, zoomed or recalled
spectra) and have a co-ordinate tag added for future reference.
Pressing the Co-ordinate Tag icon enables the QWheel™ to control the displayed cursor. With the
cursor on the required point pressing the Tag icon will add a tag at this point. Tags can be removed by
pressing the Delete Tag icon.
Auto Peak & Valley Tagging
Toolbar Icon
Peak & Valley Tagging
Peaks and valleys can be automatically identified on current, zoomed or recalled spectra. Pressing the
Peak and Valley icon will cause the scan to be re-drawn with all peaks and valleys identified with co-
ordinate tags.
The initial display is with a setting of 20% (defined as any turning point where the maximum or
minimum value reached is 20% greater or less than the previous turning point). Further presses
increase the sensitivity to 10%, 5%, 1%, 0% and finally to Off.
(60)
Overlaying Scans
Toolbar Icon
Spectral Overlay Add Scans Information
Note: Scans cannot be overlaid unless the user has signed in, as access to stored data is required.
To overlay scans first measure and store the scans for each sample required in the overlay, to a
maximum of 8 scans.
Note: The same Batch ID must be used for all of them.
Pressing the Spectral Overlay icon displays the overlay screen with the current scan.
Pressing the Add Scan icon opens a window detailing all available scans in the current batch.
Pressing the key detailing the required scan will re-draw the screen with the two scans overlaid.
Further scans from the batch can be added in a similar way.
Pressing the Information icon opens a window enabling the colours of each displayed scan to be
changed; by pressing the colour swatch next to the details of each scan it will cycle through the 8
available alternatives. Similarly, any overlaid scan can be removed by pressing the Delete icon
alongside it.
Press the Back key to exit from either screen.
(61)
Section 7
Multi-Wavelength Mode
The multi-wavelength mode is used to measure absorbance or transmittance at up to four distinct

wavelengths. There are two ways in which this mode can then be used, one sample at four
wavelengths or four samples each at a different wavelength. This mode is used for specific tests when
one sample is examined and a ratio of absorbance values (or difference between absorbance values)
at different wavelengths can reveal the purity or the composition of the sample. The ratio or difference
calculations are automatically made by the spectrophotometer. The measurements are made almost
simultaneously so the sample must be stable. Easily constructed methods for DNA or RNA
purity/concentration can be implemented, with options for automatic or manual calibration and
measurement.
Select the Multi-Wavelength Mode from the Main Menu options.
Mode settings – method name, measurement type, dwell time, reading type, sample style,
method security (if logged in)
Setting wavelengths – allows from 2 to 4 wavelengths to be set
Calculations - allows the selection of calculations and constants
Save options
(62)
7.3 Free Operation
Settings
the Multi-Wavelength mode is selected from the Main Menu:
Setting Method ID
Note: The new Method ID can only be used to identify the sample or batch on a printout to the internal
or external printer module as a single reading or a set of multiples if the Auto Log facility is used. The
generic user has no additional facility to store or recall these results or the method settings.
(63)
Selecting Sample Style
This option allows the user to select to measure one sample at up to four wavelengths, or to measure
up to four samples at different wavelengths when the 8-position cell holder is fitted. This parameter is
used in conjunction with setting wavelengths; i.e. in multiple option Sample 1 = Wavelength 1,
Sample 2 = Wavelength 2, etc, with Position 0 reserved for a common blank.
Single – allows a single sample to be measured at up to 4 wavelengths.
Multiple – allows up to 4 samples to be measured at single different wavelengths.
(64)
Setting Wavelengths
Up to four wavelengths can be set for each measurement parameter. The primary and secondary
wavelengths can be selected using any combination e.g. Wavelength #1 as the primary and
Wavelength #3 as the secondary. Values can be set by selecting the appropriate wavelength (#1-4)
and entering the new value via the numeric keypad.
The number of wavelengths to be measured can be set at 2, 3 or 4 by successive presses of the
wavelength button. Entering the minimum number for the application will speed up analysis.
Calculations
The calculations option allows the user to specify which calculations they would like to see performed
with the readings taken at selected wavelengths and with specific samples.
Sum allows the user to specify which of the following sums is performed:
A1/A2 & A1-A2 ratio and difference will allow the user to see the ratio between any two
readings – in this case the primary value divided by the secondary value, and the difference between
two readings – the primary value minus the secondary value.
(65)
(K1A1 + K2A2 + K3A3 + K4A4) x K5
K5 x (K1A1 + K2A2) / (K3A3 + K4A4)
Primary Wavelength allows the user to select which of the (up to) four wavelengths selected is to be
used as the primary wavelength in the Ratio and Difference calculation.
Secondary Wavelength allows the user to select which of the (up to) four wavelengths selected is to
be used as the secondary wavelength in the Ratio and Difference calculation. It is not possible to set
the primary and secondary wavelengths to the same value.
K1 to K5 allow the user to set the required constants for the calculations.
Setting Batch ID
The Printer Media key toggles between Internal and External Printer options.
(66)
Auto Log Setting…
Selecting the Auto Log Settings… opens a dedicated sub-menu.

This facility can be toggled between Off and On. When the Auto Log option is selected to On the
user can set the following options:
measurement screen.
(67)
pages of 8 methods.
Create a New Method
(68)
Settings
To enter the Method ID, appropriate wavelength for the sample(s) to be tested and the level of Method
Security required for the method being created select the New File icon
Setting Method ID
(69)
Selecting Sample Style
This option allows the user to select to measure one sample at up to four wavelengths, or to measure
up to four samples at different wavelengths when the 8-position cell holder is fitted. This parameter is
used in conjunction with setting wavelengths; i.e. in multiple option Sample 1 = Wavelength 1,
Sample 2 = Wavelength 2, etc, with position 0 reserved for a common blank.
Single – allows a single sample to be measured at up to 4 wavelengths.
Multiple – allows up to 4 samples to be measured at single different wavelengths.
Method Security
originator.
(70)
Setting Wavelengths
Up to four wavelengths can be set for each measurement parameter. The primary and secondary
wavelengths can be selected using any combination e.g. Wavelength #1 as the primary and
Wavelength #3 as the secondary. Values can be set by selecting the appropriate wavelength (#1-4)
and entering the new value via the numeric keypad.
The number of wavelengths to be measured can be set at 2, 3 or 4 by successive presses of the
wavelength button. Entering the minimum number for the application will speed up analysis.
Calculations
The calculations option allows the user to specify which calculations they would like to see
performed with the readings taken at selected wavelengths and with specific samples.
Sum allows the user to specify which of the following sums is performed:
A1/A2 & A1-A2 ratio and difference will allow the user to see the ratio between any two
readings – in this case the primary value divided by the secondary value, and the difference between
two readings – the primary value minus the secondary value.
(71)
(K1A1 + K2A2 + K3A3 + K4A4) x K5
K5 x (K1A1 + K2A2) / (K3A3 + K4A4)
Primary Wavelength allows the user to select which of the (up to) four wavelengths selected is to be
used as the primary wavelength in the Ratio and Difference calculation.
Secondary Wavelength allows the user to select which of the (up to) four wavelengths selected is to
be used as the secondary wavelength in the Ratio and Difference calculation. It is not possible to set
the primary and secondary wavelengths to the same value.
K1 to K5 allow the user to set the required constants for the calculations.
Setting Batch ID
accept or Cancel to remain as a Default.
The Printer Media key toggles between Internal and External Printer options.
(72)
Selecting the Auto Log Settings… opens a dedicated sub-menu.

This facility can be toggled between Off and On. When the Auto Log option is selected to On the
user can set the following options:
measurement screen.
(73)

Results Results
Saving Methods
Sharing Methods
Recalling Methods
Editing Methods
Deleting Methods
(74)
Saving Results
Printing Results
Recalling Results
Sharing Results

Deleting Results
(75)
Place the zero solution in the sample chamber and close the lid.
Pressing the Zero key will start the cycle, setting zero at each wavelength in turn. A series of
information boxes will advise of the progress.
When completed all displayed absorbance values will be set to zero (or 100% Transmittance).
Replace the zero solution with the sample to be measured and close the sample chamber lid.
Pressing the Read key will start the measure cycle, measuring at each wavelength in turn. A series of
information boxes will advise of the progress.
When completed the measured absorbances will be displayed.
The results of the selected calculation will be displayed below the Wavelength/Absorbance data.
(76)
Section 8
Kinetics Mode
A number of tests run on a spectrophotometer involve an active ingredient. On addition of this active
ingredient, the absorbance or the transmittance of the sample, will be seen to either increase or
decrease. The absorbance or transmittance is recorded at regular intervals at a preset wavelength for
a set time.
A graph is then plotted to show how the absorbance or transmittance changes over time. From the
graph, the concentration of the sample can be calculated. This mode is ideal for continuous monitoring
as well as the calculation of concentration in enzyme activity studies, with calibration possible against
a standard or known factor.
Select the Kinetics Mode from the Main Menu options:
Mode settings - method name, wavelength, measurement mode, run settings, method security
(if logged in)
Calibration – allows setting of Concentration cal standard, factor, resolution and units of
measurement
Measurement display – allows auto scaling, axis setting, colour selection
Save options

(77)
8.3 Free Operation
Settings
the Kinetics mode is selected from the Main Menu.
Setting Method ID
external printer module. The generic user has no additional facility to store or recall these results or
the method settings.
(78)
Setting Wavelength
selecting filters may be heard switching in or out).
Run Settings…
Selecting Run Settings… opens a dedicated sub-menu.
(79)
Setting Run Time
Pressing the Run Time key displays a numeric keypad. Clear the current setting and enter the
required time, in seconds, for the measurement period. The limits for this setting are from 30 to 9999
seconds (2.75 hours).
Setting Lag Time
Pressing the Lag Time key displays a numeric keypad. Clear the current setting and enter
the required lag time in seconds. The lag time is a portion of the run time over which no data is
collected, starting from the beginning of the run time. This allows for any latency or non-linear reaction
to be disregarded. Setting this to zero means data will be collected over the full run time. The limits for
this setting are from 0 to 9999 seconds.
Setting Start On Level
Pressing the Start On Level key disables the lag time option. Repeated presses of this key cycle
through the Greater Than, Less Than and Disabled options. With Greater Than or Less Than
selected data collection will start when the measured value (Absorbance or Transmittance) is above or
below the value entered in the Start Level setting.
(80)
Setting Start Level
Pressing the Start Level key displays a numeric keypad. Clear the current setting and enter the
required level above or below which data collection is required.
Limits for this setting are – 0.299 to 2.999Abs and 0.1 to 199.1%T.
Setting Concentration Parameters
The rate of change of absorbance can be converted to concentration using a known factor or
reference concentration value. Whichever is not required should be set to unity so that it is ignored in
the calculation.
Concentration Cal Standard
Select the Concentration Cal Standard key and enter the concentration value of the calibration
standard. Values from 0.01 to 9999 can be entered. If a concentration value is not to be used then this
should be set to 1.00.
(81)
Concentration Factor
This is the value by which the rate of change is multiplied to give the concentration. Select the
Concentration Factor key and enter the factor value. Values from 0.01 to 9999 can be entered. If a
Concentration Factor is not to be used this value should be set to 1.00.
Selecting Resolution
This enables the resolution of the result to be set. Resolution can be set using the Resolution key to
scroll through the available options.
Resolution options are: 1, 0.1, 0.01 and 0.001.
Selecting Units
Selection of units is made using the Units key to scroll through the available measurement options.
The following units are available:- mEq/l, ppm, mg/l, g/l, %, µg/ml, mg/ml, g/dl, mg/dl, µg/l, ng/l, µg/dl,
M, mM, µM/l, U/l, mU/lU/ml, blank.
Note: The unit is simply a name tag added to the result. Due to the variety of samples that may be
measured it is impossible to enable any direct conversion between units.
(82)
Setting Auto-Scale
will automatically set the Y-axis maxima to a level that will fit the graph of the kinetics run. When set to
Off the manual settings for Y-axis maximum and minimum are non-functional. These settings can be
changed with post run analysis tools to enable alternative views and printouts after the run has been
completed.
(83)
Setting Batch ID
and set to On the graph will be printed on one page (typically A4 landscape format) with the data on
following pages.
If available and set to Off the graph will be printed with the data below it (typically top half A4 portrait).
graph.
(84)
measurement screen.
(85)
pages of 8 methods.
Create a New Method
(86)
Setting Method ID
Setting Wavelength
selecting filters may be heard switching in or out).
(87)
Run Settings…
Selecting Run Settings… opens a dedicated sub-menu.
Setting Run Time
Pressing the Run Time key displays a numeric keypad. Clear the current setting and enter the
required time, in seconds, for the measurement period. The limits for this setting are from 30 to 9999
seconds (2.75 hours).
(88)
Setting Lag Time
Pressing the Lag Time key displays a numeric keypad. Clear the current setting and enter the
required lag time in seconds. The lag time is a portion of the run time over which no data is collected,
starting from the beginning of the run time. This allows for any latency or non-linear reaction to be
disregarded. Setting this to zero means data will be collected over the full run time. The limits for this
setting are from 0 to 9999 seconds.
Setting Start On Level
Pressing the Start On Level key disables the lag time option. Repeated presses of this key cycle
through the Greater Than, Less Than and Disabled options. With Greater Than or Less Than
selected data collection will start when the measured value (Absorbance or Transmittance) is above or
below the value entered in the Start Level setting.
Setting Start Level
Pressing the Start Level key displays a numeric keypad. Clear the current setting and enter the
required level above or below which data collection is required.
Limits for this setting are – 0.299 to 2.999Abs and 0.1 to 199.1%T.
(89)
originator.
Setting Concentration Parameters
The rate of change of absorbance can be converted to concentration using a known factor or
reference concentration value. Whichever is not required should be set to unity so that it is ignored in
the calculation.
Concentration Cal Standard
Select the Concentration Cal Standard key and enter the concentration value of the calibration
standard. Values from 0.01 to 9999 can be entered. If a concentration value is not to be used then this
should be set to 1.00.
(90)
Concentration Factor
This is the value by which the rate of change is multiplied to give the concentration. Select the
Concentration Factor key and enter the factor value. Values from 0.01 to 9999 can be entered. If a
Concentration Factor is not to be used this value should be set to 1.00.
scroll through the available options and pressing the Enter key to accept.
Selecting Units
(91)
Setting Auto-Scale
will automatically set the Y-axis maxima to a level that will fit the graph of the kinetics run. When set to
On the manual settings for Y-axis maximum and minimum are non-functional. These settings can be
changed with post run analysis tools to enable alternative views and printouts after the run has been
completed.
(92)
Setting Batch ID
and set to On the graph will be printed on one page (typically A4 landscape format) with the data on
following pages.
If available and set to Off the graph will be printed with the data below it (typically top half A4 portrait).
graph.
(93)
measurement screen.
(94)

Results Results
Saving Methods
Sharing Methods
Recalling Methods
Editing Methods
displayed touch the Settings option. Adjust the settings as required and touch the Green Tick icon on
Deleting Methods
Methods and then touch the Eraser icon.
(95)
Saving Results
Printing Results
Recalling Results
Sharing Results

Deleting Results
(96)
8.5 Performing Measurements – All Users
With a method created, or re-called and opened, the kinetics measurement screen is displayed.
Before starting a run, a zero absorbance calibration must be carried out to standardise the instrument
at the selected wavelength.
Press the Cal key and wait while the prompt advises of the calibration progress. When completed the
Run key becomes active.
Place a prepared sample cuvette in the sample chamber and close the lid. Press the Run key to start
the measurement.If a lag time has been programmed a countdown prompt will be displayed for its
duration and then the run will commence. The plot being will be displayed as the measurements are
taken over the run time.
On completion of the run a calculated concentration value is shown to the right of the graph, tagged
with the units of measurement selected. Further samples can be measured in the same way.
Post Run Tools
On completion of a measurement, or with a recalled result displayed, a number of tools can be used to
analyse and display the data.
Pressing the Tools icon on the display surround displays the bottom toolbar with icons for a number of
functions:
Pressing the Measurement Display icon gives access to options for:
Auto-Scale – pressing this icon switches Auto-Scale on and off. When Off the Y-Axis scale is
set to the manual values entered in the settings. When On it will set the Y-Axis to give the
maximum resolution for the display area.
Pressing this icon decreases the resolution of the displayed concentration, cycling through
0.001, 0.01, 0.1 and 1.
Pressing this icon increases the resolution of the displayed concentration, cycling through 1,
0.1, 0.01 and 0.001.
Pressing the information icon displays details of the curve with the mean rate of change, plus
the formula of the line of best fit.
When this option is selected the QWheel™ is activated to control the display cursors. Initially
both cursors are active to select a portion of the curve. Pressing the Cursor Select icon
activates the right and left cursor in turn to adjust the size of the selected area. The start and
end times selected by the cursors are independently displayed at the right side of the
toolbars.
By pressing the Calculator icon the concentration value is re-calculated based on the part of
the curve selected. This function can be used for method development and selecting the
optimum run times, lag and incubation periods.
(97)
Section 9
Quantitation Mode
The concentration of a substance in a sample can be found by comparing it to prepared standardised

solutions of that substance. A number of standard solutions are carefully made over a range of
concentrations. These solutions are measured by the spectrophotometer at a set wavelength and a
graph created of the concentration against the absorbance or transmittance. Samples can then be
measured in terms of absorbance or transmittance and the concentration calculated by the
spectrophotometer by comparison to the graphical plot of the known concentration standards. Linear,
quadratic and cubic curve fit functions are available, plus automation with an optional 8-cell changer
module.
Mode settings – method name, wavelength, measurement mode, resolution, units, method
Calibration – allows setting of cal standards (up to 20)
Measurement display – allows setting of curve fit, auto scaling, axis setting, colour
selection
Save options
(98)
9.3 Free Operation
Settings
the Quantitation mode is selected from the Main Menu.
Setting Method ID
external printer module. The generic user has no additional facility to store or recall these results or
the method settings.
(99)
Setting Wavelength
clear all information entered from the screen. The Del key allows individual letters or digits to be
removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The
Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths
order selecting filters may be heard switching in or out).
(100)
Selecting Units
Replicates Setup…
Selecting Replicates Setup… opens a dedicated sub-menu.

Up to 5 replicates may be selected, with the recorded result being the average of the 5 or as the last
measured result.
(101)
Selecting Calibration Standards
Up to 20 calibration standards may be used to plot the calibration curve (with up to 5 replicates for
each). Select the Number Of Standards key and enter the total number of standards to be used
(including the blank) via the numeric keypad. The value entered can be from 2 to 20. Depending on
the number entered the subsequent keys with values beyond the requirements will be greyed out.
Pressing each of the active keys below this enables the values for each standard to be input. If
working from a pre-defined calibration curve or table the corresponding absorbance value for each
standard can be entered as the result. If creating the curve from a set of standards the Results
column will be re-populated with the measured absorbances once the calibration curve has been
created.
Pressing the Cal Std 1 key opens a numeric input window. Enter the value for the first standard (this
may be the blank). When completed a corresponding absorbance value can be entered in a similar
manner by pressing the Result 1 key, if working from a pre-defined calibration curve.
Further standard (and absorbance values,

if required) can be entered in a similar
manner for all the standards selected under
Number Of Standards.
Setting Curve Fit
The Curve Fit key scrolls through the available options – Interpolation, Linear Regression, Regression
through zero, Quadratic, Quadratic through zero, Cubic, Cubic through zero. The selected curve fit
can be changed on recalled results and with post measurement tools.
(102)
Setting Auto-Scale
will automatically set the Y-axis maxima to a level that will best fit the calibration curve.
settings can be changed with post calibration analysis tools to enable alternative views and printouts
after the scan has been completed.
select the preferred colour. Once selected, the instrument will update to show the selected colour.
(103)
Setting Batch ID
and set to On the curve will be printed on one page (typically A4 landscape format) with the data on
following pages. If available and set to Off the curve will be printed with the data below it (typically top
half A4 portrait).
graph.
(104)
measurement screen.
(105)
PIN Codes – Each user is allocated a 4 digit PIN code that is required when logging in.
pages of 8 methods.
Create a New Method
(106)
Setting Method ID
(107)
Setting Wavelength
clear all information entered from the screen. The Del key allows individual letters or digits to be
removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The
Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths
order selecting filters may be heard switching in or out).
Selecting Units
(108)
Replicates Setup…
Selecting Replicates Setup… opens a dedicated sub-menu.

Up to 5 replicates may be selected with the recorded result being the average of the 5 or as the last
measured result.
originator.
(109)
Selecting Calibration Standards
Up to 20 calibration standards may be used to plot the calibration curve (with up to 5 replicates for
each). Select the Number Of Standards key and enter the total number of standards to be used
(including the blank) via the numeric keypad. The value entered can be from 2 to 20. Depending on
the number entered the subsequent keys with values beyond the requirements will be greyed out.
Pressing each of the active keys below this enables the values for each standard to be input. If
working from a pre-defined calibration curve or table the corresponding absorbance value for each
standard can be entered as the result. If creating the curve from a set of standards the Results
column will be re-populated with the measured absorbances once the calibration curve has been
created.
Pressing the Cal Std 1 key opens a numeric input window. Enter the value for the first standard (this
may be the blank). When completed a corresponding absorbance value can be entered in a similar
manner by pressing the Result 1 key, if working from a pre-defined calibration curve.
Further standard (and absorbance values,

if required) can be entered in a similar
manner for all the standards selected under
Number Of Standards.
Setting Curve Fit
The Curve Fit key scrolls through the available options – Interpolation, Linear Regression,
Regression through zero, Quadratic, Quadratic through zero, Cubic, Cubic through zero.
The selected curve fit can be changed on recalled results and with post scan measurement tools.
(110)
Setting Auto-Scale
will automatically set the Y-axis maxima to a level that will best fit the calibration curve in the display
area. When set to On the manual settings for Y-axis maximum and minimum are non-functional.
These settings can be changed with post calibration analysis tools to enable alternative views and
printouts after the scan has been completed.
select the preferred colour. Once selected, the instrument will update to show the selected colour.
(111)
Setting Batch ID
and set to On the curve will be printed on one page (typically A4 landscape format) with the data on
following pages.
If available and set to Off the curve will be printed with the data below it (typically top half A4 portrait).
graph.
(112)
measurement screen.
(113)

Results Results
Saving Methods
Sharing Methods
Recalling Methods
Editing Methods
displayed touch the Settings option. Adjust the settings as required and touch the Green Tick icon on
Deleting Methods
Methods and then touch the Eraser icon.
(114)
Saving Results
Printing Results
Recalling Results
Sharing Results

Deleting Results
Results can only be deleted by the Originator or the Supervisor using Administrative Functions.
(115)
A minimum of a zero Absorbance calibration must be made before any measurements can be carried
out. Having completed this, any pre-existing calibration curve can be used to make measurements
against, or a full calibration using the settings in the method can be made as follows:
Pressing the Cal key will display a prompt to carry out the zero absorbance. This is not part of the
calibration curve but ensures the reference levels are set correctly at this wavelength. If a blank is not
used for this setting then it should be used for Cal Std 1.
If Interpolation is used as the curve fit, then using the blank for this and Cal Std 1 will ensure the first
segment passes through the origin.
Pressing Yes completes the zero absorbance setting and is followed by a prompt to continue and
overwrite the calibration curve.
Selecting No returns the measurement screen, with the default or existing calibration curve displayed
ready for sample measurement.
Selecting Yes continues with construction of a new calibration curve and a prompt display showing
‘Please enter Standard: xx’. Where xx is the first standard in the table and could be the blank.
Pressing Yes completes the measurement of this standard.
Information on the standard and absorbance measured is then displayed along with a prompt to insert
the next standard. This process continues until all standards have been measured.
For sample measurement insert the sample cuvette into the sample chamber and press the Red key.
The measured absorbance and calculated concentration are then displayed beside the calibration
curve. Results can be printed and saved, as required.
The calibration curve is stored with all results and the method for future reference and use.
(116)
Post Quantitation Tools
After construction or recall of a calibration curve the Quantitation Tools can be enabled by pressing
the Tools icon on the display surround.
The function of these tools is as follows:
Information Auto Scale On/Off Curve Fit Curve Fit

options selection
Resolution Resolution
decrease increase
Pressing this icon displays information on the calibration curve based on the currently
selected curve fit. Constants for the curve are displayed with the correlation factors, as
applicable.
This icon turns the Auto-Scale on and off. (When set to Off manually entered values from
the settings are invoked).
Pressing this icon opens a new menu bar.
Pressing this icon cycles through the curve fit options. Exit this function by using the
Back arrow on the display surround.
Decreases the resolution of the concentration display – 0.001, 0.01, 0.1, 0.
Increases the resolution of the concentration display – 0, 0.1, 0.01, 0.001.
(117)
Fitting Additional Accessories
Integral Printer Unit
Disconnect the instrument from the mains supply.
The printer lid is located on the upper left hand side of the instrument casing. Release the lid or printer
by inserting a thin blade into the retaining tab access and then pressing both the tabs inwards.
Carefully remove the lid and retain for possible future requirements.
The printer connection lead is attached to the inside of the lid. Care should be taken when removing
the lid from the instrument to ensure no damage occurs.
Remove the printer module from the packaging and insert into the recess taking care to secure into
the retaining tabs. Detach the printer connection lead from the top of the lid and carefully fit to the
printer module.
Reconnect the instrument to the mains supply via the rear panel socket. The instrument and printer
are now ready for use.
Printer Roll Replacement
(118)
Cell Holder Accessories
A number of cell holder module options are available for use with these instruments:
- 10x10mm cell holder - 8 cell turret
- Water heated turret - Test tube holder
These are supplied as a complete plug-in module as detailed above. To fit these modules the
following procedure should be used:
Disconnect the instrument from the mains supply.
Check the installed sample chamber module and remove any filled cuvettes to avoid spillage.
Raise the lid and loosen the sample chamber retaining screw located at the front of the module. Lifting
the front carefully remove the module from the instrument and store safely for possible future use.
Unpack the new module and check to ensure no damage has occurred during transit.
To install the new module raise the lid by 30° and gently ease it into the instrument compartment
taking care that the lugs shown in the diagram locate into the main casework.
Once the module is in position, tighten the sample chamber retaining screw.
Re-connect the mains supply to the rear panel of the instrument. The instrument is now ready for use
with the new module.
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Maintenance & Troubleshooting
General
The 67 Series are designed to give optimum performance with minimum maintenance. It is only
necessary to keep the external surfaces clean and free from dust. The sample area should always be
kept clean and any accidental spillage should be wiped away immediately.
To give added protection when not in use, the instrument should be disconnected from the mains
supply and covered with the optional dust cover.
For longer-term storage or re-shipment it is recommended that the instrument should be returned to
the original packing case.
Cleaning
Note: Prior to using any cleaning or decontamination solution, except those recommended below, the
user should contact the dealer or manufacturer for advice/confirmation that the proposed method of
cleaning will not damage the instrument.
Under no circumstances should a compressed air canister be used to clean the interior surfaces of
the instrument since the resultant rapid cooling can cause damage to fragile optical components.
Casework
Do not use aggressive solvents such as acetone or abrasive cleaners for cleaning the instrument
surfaces.
For general cleaning the use of a damp cloth should be sufficient. For more thorough cleaning it is
possible to use a mild detergent solution, iso-propyl alcohol (a small amount on a cloth) or a dilute
solution of Decon 90®.
Touch Screen
The screen can be cleaned using a standard proprietary computer screen cleaner.
Note: The monochromator is a non-serviceable unit and no attempt should be made to repair this
item. Failure to observe this recommendation will result in the loss of any Warranty Claim on this
product. In the unlikely event of the monochromator requiring service or calibration, it is essential that
the manufacturer or your local dealer be contacted immediately for advice.
Light Source Replacement
The only routine maintenance that may be required is the replacement of the light source. Failure of
the lamp will be detected during the system test at power up of the instrument.
Note: The Model 6700 is fitted with a tungsten halogen lamp.

The Models 6705 and 6715 are fitted with a flash lamp module.
Tungsten Lamp Replacement – Model 6700
Warning: Disconnect the instrument from the mains supply prior to replacing the lamp. Care should
be taken when removing the lamp from the holder.
Ensure the lamp is cool prior to handling.
Access to the lamp is gained via the base of the instrument.
Important: Prior to turning the instrument upside down the sample chamber must be checked
and any samples removed to avoid spillage or damage.
Carefully turn the instrument over onto a protected work surface and locate the 2 posi-head Lamp
Module Retaining Screws and place carefully to one side, (refer second diagram).
No attempt should be made to remove the 2 socket head screws from the base.
Gently withdraw the module from the base plate and remove the Lamp Cover Retaining Screw. Place
carefully to one side.
(120)
Remove the Lamp Cover. Locate and carefully remove the tungsten lamp from the holder. The lamp is
a plug-in fit and should be removed by gently easing it from the holder.
Carefully remove the replacement lamp from the packaging, ensuring the glass portion of the lamp is
not touched.
Important: When fitting the replacement lamp it is essential that the glass envelope is not touched.
Finger marks will damage the lamp. Should accidental damage with finger marks occur, the surface of
the lamp can be cleaned using iso-propyl alcohol.
Gently insert the lamp into the holder ensuring it is fully pushed home.
Slide the Lamp Module into position in the Lamp Cover and refit the Lamp Cover Retaining Screw.
Refit the 2 posi-head Lamp Module Retaining Screws.
Carefully turn the instrument over. Reconnect the mains supply to the instrument and power up. The
self-test power up sequence will be initiated. On successful completion the instrument is ready for use.
In the unlikely event of further failure on power up self-test, please consult the manufacturer or you
local dealer for advice.
(121)
Flash Lamp Module Replacement – Models 6705 and 6715
Warning: Disconnect the instrument from the mains supply prior to replacing the lamp. Care should
be taken when removing the module. Ensure the module is cool prior to handling.
Access to the lamp module is gained via the base of the instrument.
Safety glasses must be worn when UV emissions are present.
Important: Prior to turning the instrument upside down the sample chamber must be checked
and any samples removed to avoid spillage or damage.
Carefully turn the instrument over onto a protected work surface and locate the 2 posi-head Lamp
Module Retaining Screws and place carefully to one side, (refer second diagram).
No attempt should be made to remove the 2 socket head screws from the base.
(122)
Gently withdraw the housing from the base plate and unplug the Flash Lamp Connector.
Remove the 4 Flash Lamp Retaining Screws and place carefully to one side. Locate and carefully
remove the flash lamp module.
Carefully remove the replacement lamp module from the packaging, ensuring the glass portion of the
lamp is not touched.
Important: When fitting the replacement lamp it is essential that the glass is not touched. Finger
marks will damage the lamp. Should accidental damage with finger marks occur, the surface of the
lamp can be cleaned using iso-propyl alcohol.
Gently insert the lamp module into the housing ensuring it is fully pushed home.
Refit the 4 Flash Lamp Retaining Screws. Plug in the Flash Lamp Connector.
Insert the complete assembly into the base plate and refit the 2 posi-head Lamp Module Retaining
Screws.
Carefully turn the instrument over. Reconnect the mains supply to the instrument and power up. The
self-test power up sequence will be initiated. On successful completion the instrument is ready for use.
In the unlikely event of further failure on power up self-test, please consult the manufacturer or you
local dealer for advice.
(123)
Glossary of Icons
Touch Panel Icons
Standby Instrument Settings Back
Save Toolbar Printer
Method Screens
Create a New Method
Browse Results
Touch Screen Mode Icons
Mode settings (all modes)
Accessory options (all modes)
Internal or external printer selection (Photometrics mode)
Batch ID and Auto Save (Photometrics mode)
Enter (all modes)
(124)
Analysis Points (Spectrum mode)
Measurement – auto scaling, axis setting, colour selection (Spectrum, Kinetics and
Quantitation modes)
Internal or external printer selection, graph details, Batch ID and Auto Save (Spectrum,
Multi-Wavelength, Kinetics and Quantitation modes)
Wavelength setting (Multi-Wavelength mode)
Calculations (Multi-Wavelength mode)
Calibration (Kinetics and Quantitation modes)
Spectrum Mode Scan Analysis Icons
Toggles Auto Scale (Y Axis) On or Off. If Off Y axis defaults to manual settings entered in
the method.
Cycles round Plot Interval settings of 0.1, 0.5, 1.0 and 5.0nm
Zoom
Activates the zoom area select cursors that can be positioned using the QWheel™ over
the area required. Repeated presses increase the zoom up to 5 times.
Returns the zoom to the next lower level. At x1 this icon is disabled.
(125)
Exit Door maintains the zoom display and gives access to the tools for manipulation
and analysis of the zoomed portion.
Area Under Curve Cursor Select
Co-ordinate Tagging Tag Delete Tag
Peak & Valley Tagging
Spectral Overlay Add Scans Information
Quantitation Mode Analysis Icons
Information Auto Scale On/Off
Curve Fit Curve Fit options selection
Conc. resolution decrease Conc. resolution increase
(126)

JEW031 - EN Spectro 6700 Series Manual

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67 Series

670 005/REV A/08-07

Models 6700, 6705 & 6715

670 005/REV A/08-07

Please read this important guarantee information:

Returned goods will not be processed without a Returns Authorisation Number.

Section 1 Introduction Page

Section 2 Getting Started

Section 3 Systems of Operation

Section 4 Instrument Settings

Section 5 Photometrics Mode

Section 6 Spectrum Mode

Section 7 Multi-Wavelength Mode

Section 8 Kinetics Mode

Section 10 Fitting Alternative Modules/Accessories

Section 11 Maintenance & Troubleshooting

Glossary of Icons 124-126

1.1 Instrument Description

6700 6705 6715

Light Source Tungsten Halogen Xenon Xenon

Optics Sealed, MgF Coated, Split Beam

Environmental Operating Conditions:

The 67 Series is designed to work safely under the following conditions:

Range Ambient +2°C to 60°C

b) Plastic disposable cuvettes should be used ONCE only.

6) Cold samples should be allowed to equilibrate to ambient temperature before measurement

2.1 Unpacking Instructions

• Refer to label 1 on carton exterior and

• Check each item as it is removed from

• Remove Documentation carton 2 .

• Please take some time to familiarise

• Remove the cardboard packing piece 3

• Remove the two foam packing pieces 4

• Grasp the instrument 5 (which will be

• Remove the Accessory carton 6 . Note

2.22 Supply Voltage

2.23 Mains Connections

3. Back key – this is used to return to a previous menu level.

Create a New Method

Open the Selected Method

Erase the Selected Method

Browse Results – linked to the selected method

Open specific results in the selected batch

Accessory options – varies with type of accessory module fitted

Allows selection of internal or external printer

Allows set-up of batch ID and the Auto Save options

Press to accept settings entered

Analysis Points – up to 30 wavelengths at which absorbance will be reported

Measurement display – auto scaling, axis setting, colour selection

Accessory options – varies with type of accessory module fitted

Press to accept settings entered

Setting wavelengths – allows from 2 to 4 wavelengths to be set

Calculations - allows the selection of calculations and constants

Accessory options – varies with type of accessory module fitted

Press to accept settings entered

Measurement display – allows auto scaling, axis setting, colour selection

Accessory options – varies with type of accessory module fitted

Press to accept settings entered