English

SOP
0623

Transferpette® single
channel
Standard Operating Procedure
 Table of contents

1 Introduction ................................................................................................................................................. 3

2 Preparation .................................................................................................................................................. 4
2.1 Instrument type and serial number ................................................................................................................................. 4
2.2 Minimum required equipment ......................................................................................................................................... 4
2.3 Cleaning ............................................................................................................................................................................. 4
2.4 Visual inspection for damage and leaks........................................................................................................................... 4
2.5 Functional test................................................................................................................................................................... 5

3 Test instruments and accessories ................................................................................................................... 6

4 Gravimetric testing ....................................................................................................................................... 7

4.1 For Transferpette® with nominal volume > 50 μl............................................................................................................. 7
4.2 For Transferpette® with nominal volume ≤ 50 μl............................................................................................................. 8

5 Evaluation of gravimetric test results ............................................................................................................. 10

5.1 Possible volume error ....................................................................................................................................................... 11
5.2 Temperature and factor Z................................................................................................................................................. 11
5.3 Manufacturer error limits for single-channel pipettes .................................................................................................... 12
5.4 ISO error limits for pipettes .............................................................................................................................................. 13
5.5 Error limits to be defined by the user............................................................................................................................... 13

6 Test report for volumetric instruments ........................................................................................................... 14

7 Appendix ...................................................................................................................................................... 16
7.1 Abbreviations, units, and notations ................................................................................................................................. 16
7.2 Declaration on the Absence of Health Hazards ............................................................................................................... 17
7.3 BRAND Calibration Service ............................................................................................................................................... 18
7.3.1 Range of instruments.......................................................................................................................................... 18
7.3.2 Testing in accordance with DIN EN ISO 8655..................................................................................................... 18
7.4 Accredited calibration laboratory D-K-18572-01-00 by BRAND ...................................................................................... 18
7.4.1 Volumetric instruments with DAkkS calibration certificates issued by BRAND............................................... 19
7.5 EASYCAL™ Calibration software – test equipment monitoring made easy.................................................................... 20

www.brand.de
Standard Operating Procedure English

1. Introduction

The test instruction transfers standards relevant to the test into a practical form. It can therefore be used as a basis for test equip-
ment monitoring according to DIN EN ISO 9001, DIN EN ISO 10012, and DIN EN ISO/IEC 17 025.

Basically, we recommend an inspection every 3…12 months. However, the testing interval may be adjusted to your individual re-
quirements. In the case of high frequency of use or the use of aggressive media, it is advisable to check more frequently.

The following instruments can be checked using these test instructions:

Instruments Types Relevant standards

Transferpette® Fixed volume ISO 8655:2022

Transferpette® S Variable volume
Transferpette® electronic

For the regular checks according to DIN EN ISO 9001, DIN EN ISO 10 012, and DIN EN ISO/IEC 17 025 as well as the GLP Guidelines,
we offer a calibration service (see 'BRAND Calibration Service, p. 18'). This calibration service saves you time and internal effort,
especially if you still have to perform calibrations in addition to ongoing operation.

Legend

In order to simplify the collection of the relevant data, the SOP refers to the respective items in the test report. The following
graphics indicate to these positions:

Example:

Position in the test report:

1
Instrument

In the appendix, you will also find the health clearance form required to send in equipment as well as information about our ac-
credited calibration laboratory and EASYCAL™ 5 calibration software.

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Standard Operating Procedure English

2. Preparation

2.1. Instrument type and serial number

1. Determine instrument type and nominal volume. Enter in the test report: 1

2. Read off the serial number. The serial number is located on the Hand grip . Enter in the test report: 1

3. Read customers identification, if available. Enter in the test report: 1

2.2. Minimum required equipment

You will need:

+ Transferpette ®

+ Use suitable pipette tips.

The best results are achieved with original BRAND pipette tips.

2.3. Cleaning
+ Recommendation:
Calibrate instrument before cleaning (actual value), and clean afterwards if necessary.

+ Clean the pipette shaft.

Make sure there are no media residues.
Wipe the outside with a soft cloth!

+ Clean the housing adequately.

Minor soiling permissible!

+ Liquid residues in the instrument?

Disassemble and clean the instrument.
See instructions for use.

2.4. Visual inspection for damage and leaks

+ Housing: general damage?

+ Pipette shaft tip: scratches on the surface?

+ Ejector unit

+ Piston: scratches or dirt on the surface?

+ Seal: scratches or dirt on the surface?

Enter the result in the test report 2 .

Possible errors and the actions that can be taken as a result:

Error Possible causes Measures
Pipette tip no longer seals Scratch on the pipette shaft tip Procure spare parts; see instructions for
use
Instrument is sluggish or leaking Seal/piston soiled or damaged Procure spare parts; see instructions for
use

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Standard Operating Procedure English

2.5. Functional test

1. Attach a new pipette tip.

2. Setting the nominal volume

3. Take up test liquid. Absorption of liquid not possible or very slow: note the information in the following table.

4. Test with BRAND leak tester PLT unit or hold pipette vertically for approx. 10 s and observe whether a drop forms at the
pipette tip.

→ If an error appears in the display of the PLT unit or a drop forms at the pipette tip, note the information in the following table.

5. Dispense test liquid. Hold the pipette tip against the vessel wall and wipe it off.

6. The pipetting knob must move smoothly and without jerks.

7. Eject tip Enter the result in the test report 3 .

Possible errors and the actions that can be taken as a result:

(Measures for correcting other errors can be found in the respective instructions for use)

Error Possible cause Measures

Suction not possible or very slow. Pipette shaft or pipette shaft tip clogged Carry out cleaning; see instructions for
use
“Error” when testing with PLT unit or + Pipette tip not properly attached. + Use new pipette tips, and attach
droplet forms at pipette tip. them firmly.
+ Seal or piston defective.
+ Clean or replace the seal or piston;
see instructions for use.

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Standard Operating Procedure English

3. Test instruments and accessories

+ A test room with the following features:

draft-free
low temporal and spatial temperature fluctuations
Taking into account the measuring tolerance of the hygrometer, a relative humidity of 45…80% should be reached.
Ambient temperature of max. 20 ± 3°C

+ Place the instrument to be tested with accessories unpacked in the test room for at least 2 h so that the instrument and ac-
cessories can adjust to the ambient temperature.

+ A recipient vessel filled with deionized or distilled water (e. g. Erlenmeyer flask) (water quality in accordance with ISO 3696,
at least quality 3) Consider the following aspects:
Adjust the water and ambient temperature by max. 0.5°C.
Prevent the water in the vessel from cooling down as a result of evaporation.

+ Provide a weighing vessel (e.g., Erlenmeyer flask). Fill this with a small amount of water.
Make sure at least the bottom is covered.
Provide evaporation protection for < 100 μl test volume.

+ Measuring instruments in accordance with DIN ISO 8655-6:

Instrument Resolution:
Thermometer for liquids 0.1°C
Thermometer for ambient temperature 0.1°C
Hygrometer 1% relative air humidity
Barometer 0.1 kPa
Timer 1s

+ Balance in accordance with DIN EN ISO 8655-6:

Nominal volume of the instrument to be tested Resolution of the display Repeatability and linearity a

V mg mg
b
0.5 ≤ V < 20 µl 0.001 0.006 b
20 µl ≤ V < 200 µl 0.01 0.025
200 µl ≤ V ≤ 10 ml 0.1 0.2
a
The repeatability in this table applies to the volume determination of a single-channel instrument. If a single-channel balance is
used exclusively for the volume determination of multichannel pipettes, the repeatability is twice as high as specified in this ta-
ble.
b
single channel balance

+ Accessories (for pipettes with a nominal volume ≤ 50 μl):

Disposable micropipettes intraEND 100 μl; Order no. 709144
Pipette holder, Order no. 708605
Micro-weighing vessel, Order no. 708470

Traceability of test results to the national standard

By using calibrated test equipment (balance and thermometers), the requirement of DIN EN ISO 9001, DIN EN ISO 10 012,
and DIN EN ISO/IEC 17 025 for traceability of the test to the national standard is fulfilled. The calibration of the balance can
be done by DAkkS calibration, a direct official calibration of the balance, or by calibrating the balance with correspondingly
traced weights (corresponding precision). The thermometer can also be calibrated by means of a DAkkS calibration, an offi-
cial calibration, or by comparison with corresponding traceable thermometers (under defined conditions).

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Standard Operating Procedure English

4. Gravimetric testing

The following sections describe how to perform gravimetric testing. If you wish to perform the test, follow the procedure appro-
priate for your test instrument. As an aid, document their recorded results in the test report. The identifiers ( Ex. 1 … 6 ) refer
to the respective location in the test report.

4.1. For Transferpette® with nominal volume > 50 μl

1. Set 10 or 20% of the nominal volume.

2. Determine the temperature of the deionized water. Enter the result in the test report 4 .

3. Place the weighing vessel (containing a small amount of deionized water) on the balance and tare the balance.

4. Attach a new pipette tip.

Perform conditioning:
Take up and dispense test liquid five times.
Conditioning increases the precision of the test.

5. Take up the test liquid from the receiving vessel.

→ Press the pipetting button to the first stop (not applicable for Transferpette® electronic), and allow the pipetting button to
slide back slowly and evenly. For Transferpette® electronic, press the pipetting button to take up liquid. Observe waiting
times and immersion depths depending on the volume range; see table below.

6. Remove the weighing vessel from the balance.

→ not applicable if special pipette calibration balance is used.

7. Dispense the sample into the weighing vessel.

→ Place the pipette tip at an angle of 30…45° to the vessel wall.

→ For the mechanical Transferpette®, press the pipetting button at a steady speed to the first stop, and hold it down.
For the electronic Transferpette®, press and hold the pipetting key.

→ Empty the pipette tip completely by pressing all the way down (takes place automatically with the Transferpette® elec-
tronic).

→ Wipe the pipette tip on the vessel wall over a length of about 10 mm.
→ Allow the pipetting button of the Transferpette® to slide back evenly. Release the pipetting button of the Transferpette® elec-
tronic.

8. Place the weighing vessel on the balance, and note the weight value.

→ Enter the result in the test report 5 .

9. Re-tare the balance.

10. Perform points 5 through 9 ten times each.

→ Enter the result in the test report 5 .

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Standard Operating Procedure English

→ During these 10 measurements, change the tip at least once. In this case, pipetting must be started at Point 4.

11. Then pipette analogously at 50% and 100% of the nominal volume.

12. Start at Point 4 in each case.

→ Only for variable and electronic instruments.

→ Enter the result in the test report 5 .

→ 30 weight values are determined for the instrument with variable volume adjustment and the electronic instrument. For the
instruments with fixed set volume, 10 weight values are determined. Use the report to check whether the test was performed
completely

Table: Immersion depth and waiting time

Volume [µl] Immersion depth [mm] Wait time [s]

≤1 1…2 1
> 1…100 2…3 1
> 100…1,000 2…4 1
> 1,000…20,000 3…6 3

4.2. For Transferpette® with nominal volume ≤ 50 μl

Note on evaporation
For pipettes with a nominal volume ≤ 50 μl, the tolerance limits are usually smaller than 0.5 μl. This low tolerance limit
means that the evaporation of water during the test has a relatively high influence on the measurement result. For this rea-
son, a test method that largely prevents evaporation must be used for pipettes ≤ 50 μl. If a special pipette calibration bal-
ance with an “evaporation trap” is used, carry out the procedure as in 'For Transferpette® with nominal volume > 50 μl, p. 7' .
BRAND has developed a new test method especially for this purpose. The weighing vessel used is a disposable micropipette
or a micro-weighing vessel; these allow almost no evaporation.

1. Set 10 or 20% of the nominal volume.

2. Determine the temperature of the deionized water. Enter the temperature into the test report 4 .

3. Attach a new pipette tip. Perform conditioning: Take up and dispense test liquid five times. Conditioning increases the preci-
sion of the test.

4. Clamp the disposable micropipette to the pipette holder, place it on the balance, and tare the balance or the micro-weighing
vessel.

5. Take up the test liquid from the receiving vessel.

→ Press the pipetting button to the first stop (not applicable for Transferpette® electronic), and allow the pipetting button to
slide back slowly and evenly. For Transferpette® electronic, press the pipetting button to take up liquid. Observe waiting
times and immersion depths depending on the volume range; see table below.

6. Remove the disposable micropipette or micro-weighing vessel from the balance.

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Standard Operating Procedure English

→ The pipette holder facilitates handling.

7. Dispense the sample into the weighing vessel. Dispense the sample into the disposable micropipette or micro-weighing ves-
sel.

→ Attach the disposable micropipette as far as possible to the pipette tip or insert the pipette tip into the cone of the micro-
weighing vessel.

→ Press and hold the pipetting button on the Transferpette® at a steady speed until the first stop; press and hold the pipetting
button on the Transferpette® electronic.

→ Empty the pipette tip completely by pressing all the way down (takes place automatically with the Transferpette® elec-
tronic). An air bubble forms in the disposable micropipette.

→ Remove the disposable micropipette or micro-weighing vessel from the pipette tip while pressing all the way down.
→ Allow the pipetting button of the Transferpette® to slide back evenly. Release the pipetting button of the Transferpette® elec-
tronic.

8. Place the disposable micropipette or micro-weighing vessel on the balance; note the weight value.

→ Enter the result in the test report 5 .

9. Re-tare the balance.

10. Perform points e) through h) 10 times.

→ Enter the weight values into the test report 5 .

11. Then pipette analogously at 50% and 100% of the nominal volume.

12. In each case, start at d).

→ Only for variable and electronic instruments.

→ Enter the results in the test report 5 .

→ This results in 30 weight values (variable and electronic instrument) or 10 weight values (fixed instrument).

Table: Immersion depth and waiting time

Volume [µl] Immersion depth [mm] Wait time [s]

≤1 1…2 1
> 1…100 2…3 1
> 100…1,000 2…4 1
> 1,000…20,000 3…6 3

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Standard Operating Procedure English

5. Evaluation of gravimetric test results

The weight values obtained during the gravimetric test are only the mass values of the dispensed volume. In order to obtain the
actual volume, an adjustment calculation must be carried out. The following calculations must be carried out:

Step Calculation Remark

1. Mean weight:
(Example for 10 weight values)

2. Mean volume: Factor Z (see Table 1).

Enter the result in the test report 6a .

3. Standard deviation: Factor Z (see Table 1).

Enter the result in the test report 6b .

4. Accuracy: Enter the result in the test report 6c .

5. Coefficient of variation: Enter the result in the test report 6d .

–
V–V Sollwert
Actual/nominal value comparison:
. 100 Enter the result in the test report 6e .
R [%] =
For error limits, see 'Manufacturer error limits for single-channel pipettes,
V
p. 12' and 'ISO errorSollwert
limits for pipettes, p. 13' and the following accuracy
tables for the respective instrument, or define your own error limits.
Result: Enter the result in the test report 6g .

s . 100
If the calculated
VKvalues
[%] = (A% and
– CV%) are less than or equal to the error limits, the instrument is in good working order.
V

If the calculated values are greater than the error limits:

+ Check that all instructions have been carried out correctly.

+ Follow the instructions in the “Troubleshooting” section of the instructions for use.

+ Adjust the Transferpette®-8/-12, Transferpette®-8/-12 electronic, or Transferpette® S -8/-12 according to the instructions for
use.
If these measures do not lead to success, we recommend that you use the BRAND calibration service (see 'BRAND Calibration Ser-
vice, p. 18').

We recommend using software to help perform the calculation and evaluation. For this purpose, BRAND offers the EASY-
CAL™ calibration software (see here). This convenient software runs on Windows and speeds up the calculation consider-
ably.

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Standard Operating Procedure English

5.1. Possible volume error

Possible volume errors and the actions that can be taken:

Error Possible causes Measures

Volume too small Pipette tip not properly attached Use new pipette tip, and attach firmly.
Seal or piston defective Clean or replace the seal or piston; see in-
structions for use
Pipette shaft tip no longer screwed on tightly; red marking Retighten pipette shaft tips
may be visible (not possible with Transferpette® electronic
and Transferpette® S)
Volume too large Pipetting button pressed too far Pay close attention to the first stop
Other influencing vari- Instrument incorrectly adjusted Readjust instrument
ables Temperature calibration of instrument, ambient, and water Perform temperature adjustment
temperature not completed

5.2. Temperature and factor Z

Extract from DIN EN ISO 8655
Table refers to 1,013 hPa.
In the validity range from 950 hPa to 1040 hPa.

Temperature: Factor Z Temperature: Factor Z

°C ml/g °C ml/g
15 1.0020 23 1.0035
15.5 1.0020 23.5 1.0036
16 1.0021 24 1.0038
16.5 1.0022 24.5 1.0039
17 1.0023 25 1.0040
17.5 1.0024 25.5 1.0041
18 1.0025 26 1.0043
18.5 1.0026 26.5 1.0044
19 1.0027 27 1.0045
19.5 1.0028 27.5 1.0047
20 1.0029 28 1.0048
20.5 1.0030 28.5 1.0050
21 1.0031 29 1.0051
21.5 1.0032 29.5 1.0052
22 1.0033 30 1.0054
22.5 1.0034

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Standard Operating Procedure English

5.3. Manufacturer error limits for single-channel pipettes

Transferpette® S, fixed volume

Volume range *R* ≤ ± % CV* ≤ % Recommended tip type
[µl] [μl]
10 1 0.5 0.5–20
20 0.8 0.4 2–200
25 0.8 0.4 2–200
50 0.8 0.4 2–200
100 0.6 0.2 2–200
200 0.6 0.2 2–200
500 0.6 0.2 50–1000
1000 0.6 0.2 50–1000
2000 0.8 0.3 500–5000

*A = Accuracy, CV = Coefficient of Variation

Transferpette® S, variable volume

Volume Partial *R* ≤ ± % CV* ≤ % Sub Recommended tip type
range volume steps [μl]
[µl] [μl] [μl]
0.1–1 1 2 1.2 0.001 0.1–20
0.5 4 2.4
0.1 20 12
0.1–2.5 2.5 1.4 0.7 0.002 0.5–20
1.25 2.5 1.5
0.25 12 6
0.5–10 10 1 0.5 0.01 0.5–20
5 1.6 1
1 7 4
2–20 20 0.8 0.4 0.02 2–200
10 1.2 0.7
2 5 2
5–50 50 0.8 0.3 0.05 2–200
25 1.2 0.5
5 4 2
10–100 100 0.6 0.2 0.1 2–200
50 0.8 0.4
10 3 1
20–200 200 0.6 0.2 0.2 2–200
100 0.8 0.3
20 3 0.6
100–1000 1000 0.6 0.2 1 50–1000
500 0.8 0.3
100 3 0.6
500–5000 5000 0.6 0.2 5 500–5000
2500 0.8 0.3
500 3 0.6
1000–10,000 10000 0.6 0.2 10 1000–10000
5000 0.8 0.3
1000 3 0.6

*A = Accuracy, CV = Coefficient of Variation

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Standard Operating Procedure English

Transferpette® electronic

Volume Partial A* ≤± % CV* ≤% Sub Recommended tip type

range volume steps [μl]
[µl] [μl] [μl]
0.5 - 10 10 1.0 0.4 0.01 0.5 - 20
5 1.5 0.8
1 5.0 2.0
2 - 20 20 1.0 0.4 0.02 0.5 - 20
10 1.5 0.8
2 5.0 2.5
10 - 200 200 0.8 0.2 0.2 2 - 200
100 1.2 0.3
20 4.0 0.6
50 - 1000 1000 0.6 0.2 1.0 50 - 1000
500 1.0 0.3
100 3.0 0.6
250 - 5000 5000 0.6 0.2 5.0 500 - 5000
2500 1.0 0.3
500 3.0 0.6

*A = Accuracy, CV = Coefficient of Variation

5.4. ISO error limits for pipettes

Nominal volume A≤±% CV ≤ %

1 to 3 µl 2.5 2
> 3 to 5 µl 2.5 1.5
> 5 to 10 µl 1.2 0.8
> 10 to 50 µl 1.0 0.5
> 50 to 5,000 µl 0.8 0.3
> 5,000 to 10,000 µl 0.6 0.3

5.5. Error limits to be defined by the user

For calibration, the applicable error limits must be defined by the user. Different methods can be applied to accomplish
this:
If the application requires it and the optimized test conditions exist for measurement, the error limits specified in the 'Man-
ufacturer error limits for single-channel pipettes, p. 12' can also be expected in the case of used, intact volumetric instru-
ments.
In accordance with the German Calibration Law, however, it is also admissible to apply operational limits. The operational
limits equate to double the calibration error limits. This means that the values of the manufacturer’s error limits', p. 12'
must be doubled. Users may also define their own individual error limits related to their particular application, which their
calibrated (adjusted) measuring instrument should adhere to.
This procedure meets the requirements of DIN EN ISO 9001, DIN EN ISO 10 012, and DIN EN ISO/IEC 17 025.

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Standard Operating Procedure English

6. Test report for volumetric instruments

1 Instrument

Titrette® Type Nominal volume:

Burette Digital fixed
Dispensette® variable Serial number:
Transferpette®
Transferpette® S analog Customer’s marking:
Transferpette® electronic digital
Transferpettor

2 Damage

Nominal volume:

Serial number:

Customer’s marking:

3 Operating defects

Type of damage

Damage remedied
none
Type of functional defect
Functional defect eliminated

4 Environment

Water temperature:

Balance:

Thermometer:

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Standard Operating Procedure English

Continued on next page Relative humidity: (at least 45%):

Correction factor Z:

5 Weight values of the gravimetric test

Weight value No. At 10% in mg At 50% in mg For nominal volume in mg

x1:

x2:

x3:

x4:

x5:

x6:

x7:

x8:

x9:

x10:

6 Evaluation of the gravimetric test

Calculated value At 10% At 50% For nominal volume

6a

6b s
6c A [%] actual
6d CV [%] actual
6e A [%] target
6e CV [%] target
6g Result

The test was carried out according to DIN EN ISO 8655 and DIN EN ISO 4787.

Date: Signature:

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Standard Operating Procedure English

7. Appendix

7.1. Abbreviations, units, and notations

The following abbreviations are used in this or other test instructions:

Symbol A < B: A is less than B

A ≤ B: A is less than or equal to B

Ranges Example: 980…1,000 hPa

Prevents sign confusion (i.e., en dash as minus sign)

Example: 20 µl < V < 100 µl

The volume V is between 20 µl and 100 µl (V is larger than 20 µl and smaller than 100 µl).

Materials PFP: perfluorinated pentacene

PMP: polymethylpentene
PFA: perfluoroalkoxy polymer
Boro 3.3: borosilicate glass
AR-GLAS®: A soda-lime glass from SCHOTT AG, 55122 Mainz, Germany
PUR: polyurethane

W1 Tare weight of the weighing vessel

W2 Weight of the weighing vessel filled with the medium to be weighed.
A Correctness
CV Coefficient of variation:

V Volume
s Second
l Liter
ml Milliliter(s)
µl Microliters
g Gram(s)
mg Milligrams(s)

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Standard Operating Procedure English

7.2. Declaration on the Absence of Health Hazards

Please enclose with the instrument or send as an e-mail to service@brand.de.
BRAND GMBH + CO KG
Otto-Schott-Str. 25
97877 Wertheim
service@brand.de
F +49 9342 808 91290

We are required by law to protect our employees against hazards caused by contaminated instruments. We therefore ask
for your understanding that we do not perform calibrations | Repairs can be carried out only if we have received this decla-
ration completely filled in and signed.

ATTENTION: If you are a customer outside of Germany, please contact our local service partner in your country. Please send in instruments from outside Germany only after being requested to do so. Unsolicited instruments

cannot be processed.

To the instrument shipment from | To delivery slip number

The undersigned hereby declares:

+ That the instruments have been carefully cleaned and decontaminated before shipment.

+ That the instruments pose no danger through bacteriological, viral, chemical, and/or radioactive contamination.

Applications:

Media used:

Acids Other:
Bases
Solvents
Serum, blood

Decontamination measures:

Company / laboratory (official stamp) Name:

Pos.

Date / legally binding signature:

Tel. / fax / e-mail __________________________________

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Standard Operating Procedure English

7.3. BRAND Calibration Service

BRAND offers a complete service that includes calibration and adjustment of BRAND and third-party instruments as well as any
necessary maintenance and repair of BRAND instruments. This saves time and money, with the added benefit of testing by an in-
dependent laboratory. Find more information and the order form for the repair and calibration service on brand.de.

7.3.1. Range of instruments

1. Piston-operated pipettes (single- and multi-channel)
2. Bottle-top dispensers
3. Bottle-top burettes
4. Repetitive pipettes

7.3.2. Testing in accordance with DIN EN ISO 8655

A team of qualified staff, working in temperature and humidity controlled rooms and using state-of-the-art balances and calibra-
tion software, calibrates Liquid Handling instruments, regardless of their make, in accordance with DIN EN ISO 8655.
Variable volume instruments such as the HandyStep®Touch, HandyStep®Touch S, HandyStep® electronic, Transferpette®, Trans-
ferpette®S, Transferpette®electronic, Transferpette®-8/-12, Transferpette®-8/-12 electronic, Transferpette®S -8/-12, Transferpet-
tor, Dispensette®, digital burettes, or Titrette® are checked at nominal volume, 50% of the nominal volume, and at 10% or 20% of
the nominal volume.
To document the results, a detailed test report that fully complies with all relevant regulations is compiled.
The BRAND Calibration Service provides:
1. Calibration of Liquid Handling instruments, regardless of their make
2. Detailed calibration certificate
3. Return of instrument within a few working days
4. Cost-efficient implementation

7.4. Accredited calibration laboratory D-K-18572-01-00 by BRAND

Precise measurement results are essential in all areas – both for internal quality assurance and to meet various standard require-
ments.
BRAND has been accredited since 1998 – first by the DKD (German Calibration Service) and since 2013 by the DAkkS (German Ac-
creditation Body) as a calibration laboratory for volumetric instruments in accordance with DIN EN ISO/IEC 17025.
Thanks to these many years of experience in the calibration
of volumetric instruments as well as liquid handling instru-
ments, customers also find BRAND a trustworthy service
provider for test equipment monitoring
Standards (e.g., DIN EN ISO 9001 and DIN EN ISO/IEC 17 025)
require that measured values are metrologically traceable to
international units. Proof of this is provided by calibration
certificates issued by accredited laboratories (often also
called DAkkS or DKD calibration certificates).
With the calibration certificate in accordance with DIN EN ISO/IEC 17025, our customers receive a calibration that is internation-
ally recognized as metrologically traceable in many countries. This is possible thanks to the membership of DAkkS in the EA (Eu-
ropean Cooperation for Accreditation) and ILAC (International Laboratory Accreditation Cooperation).

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Standard Operating Procedure English

Calibration certificate according to BRAND accreditation certificate

DIN EN ISO/IEC 17025

BRAND performs the calibration of liquid handling equipment in accordance with the gravimetric reference method in compli-
ance with all requirements of DIN EN ISO 8655-6:2022.
For volumetric instruments made of glass or plastic, we work in accordance with DIN EN ISO 4787:2022 or, if necessary, in accor-
dance with accredited in-house procedures.
As a rule, and unless otherwise requested by our customers, our calibration results are assessed for conformity on the basis of the
ILAC-G8:03/2009 decision rule. For this purpose, the measurement result is evaluated taking into account the expanded measure-
ment uncertainty with 95% overlap probability in relation to relevant standard or manufacturer tolerances. This provides our
customers with good assistance in assessing whether the test equipment meets their own quality requirements.

7.4.1. Volumetric instruments with DAkkS calibration certificates issued by BRAND

BRAND calibrates the following volumetric instruments (new or already in use and regardless of make):

+ Piston-operated pipettes, from 0.1 µl to 10 ml

+ Multi-channel piston-operated pipettes from 0.1 µl to 300 µl

+ Piston-operated burettes, from 5 µl to 200 ml

+ Dispensers and dilutors from 5 µl to 200 ml

+ Glass volumetric instruments, adjusted to contain (TC, In) from 1 µl to 10,000 ml

+ Glass volumetric instruments, adjusted to deliver (TD, Ex) from 100 µl to 100 ml

+ Plastic volumetric instruments, adjusted to contain (TC, In) from 1 ml to 2,000 ml

+ Plastic volumetric instruments, adjusted to deliver (TC, Ex) from 1 ml to 100 ml

+ Glass pycnometers, from 1 cm3 to 100 cm3

www.brand.de 19
Standard Operating Procedure English

7.5. EASYCAL™ Calibration software – test equipment monitoring made easy

The EASYCAL™ 5 calibration software facilitates the monitor-
ing of liquid handling instruments (piston-stroke instruments
such as pipettes, dispensers, burettes, and manual dis-
pensers) as well as volumetric instruments made of glass or
plastic according to GLP/GMP and DIN EN ISO 9001. EASY-
CAL™ 5 can be used not only for BRAND instruments but also
for the instruments of all manufacturers.
EASYCAL™ 5 performs all calculations automatically and com-
pares them with the tolerances from the current standards or
their individually defined limits. The tolerances of numerous
instruments and the interface settings of over 100 test instru-
ments (e.g., balances) are already stored for you.
Choose between a stand-alone version for working on one workstation (recommended for small laboratories where calibration is
done by a single person) or a client/server version for parallel, distributed work on multiple workstations (floating licenses are in-
stalled on the server).

Functions:
+ Testing of liquid handling instruments and volumetric instruments made of glass and plastic in accordance with ISO 8655,
ISO 4787, and others.

+ Open software, suitable for all volumetric instruments – regardless of manufacturer.

+ Extensive library with instrument specifications from well-known manufacturers can be expanded and modified by the user.

+ Scope of testing can be individually defined by the user via test plans. An extensive library of test plans is included to help
you get started with EASYCAL™ 5 and minimize data entry time.

+ Instrument management – quickly and easily search and find the owner, test history, and next test date.

+ Continuous control of the current actual state during the test by graphical representations and ad hoc calculation of statisti-
cal values.
Reminder function for upcoming tests with automatic notification of the instrument owner via e-mail.

+ Integration of the address data of your customers and suppliers in a business partner database User administration with user
roles (e.g., auditor, supervisor, system administrator) and access restriction to EASYCAL functions.
Dual-control principle for the release of critical data such as test plans, calibration orders before certificate printing, and in-
strument specification.

+ Interface connection via RS232 of measuring instruments such as balances, thermometers, barometers, and hygrometers
with automatic transmission of the measured values.

+ In the integrated certificate editor, you can customize the certificates, and test reports supplied to your needs and create the
design.

www.brand.de 20
BRAND GMBH + CO KG
Postfach 1155 | 97861 Wertheim | Germany
T +49 9342 808 0 | F +49 9342 808 98000 | info@brand.de | www.brand.de

BRAND.For lab.For life®

© 2023 BRAND GMBH + CO KG | Printed in Germany | 0623

BRAND®, BRAND. For lab. For life.® and the word/image mark BRAND are trademarks or registered
trademarks of BRAND GMBH + CO KG, Germany. All other trademarks shown or reproduced are the
property of their respective owners.

We want to inform and advise our customers through our technical writings. However, the transfer-
ability of general empirical values and results under test conditions to the specific application de-
pends on a variety of factors beyond our control. We therefore ask for your understanding that no
claims can be derived from our advice. The transferability must therefore be checked very carefully
by the user himself in each individual case.

Technical changes, errors and misprints excepted.

At store.brand.de you will find For more information on prod-

accessories and spare parts, ucts and applications, please
user manuals, test instructions visit our Youtube channel my-
(SOP) and videos for the prod- labBRAND.
uct.

BRAND (Shanghai) Trading Co., Ltd. BRAND Scientific Equipment Pvt. Ltd. BrandTech® Scientific, Inc.
Shanghai, China Mumbai, India Essex, CT. United States of America

Tel.: +86 21 6422 2318 Tel.: +91 22 42957790 Tel.: +1 860 767 2562
info@brand.com.cn customersupport@brand.co.in info@brandtech.com
www.brand.cn.com www.brand.co.in www.brandtech.com