Scribd Logo
Upload

Welcome to Scribd!

  • 7 views

    SEM Instructions

    Uploaded by

    drnjkaur

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    SEM Instructions

    Uploaded by

    drnjkaur
    7 views2 pages

    Original Title

    SEM instructions

    Copyright

    © © All Rights Reserved

    Available Formats

    DOC, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    7 views2 pages

    SEM Instructions

    Uploaded by

    drnjkaur

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    You are on page 1of 2

    Recommended centering conditions

    The centering is always done by


    means of a special centering
    specimen, which is included in the
    microscope accessories and is marked
    as ADJ. The surface of this specimen
    has a fine structure, making the
    centering easier. If this specimen is not available, it is possible to use a common specimen stub. Once
    you start centering, the image must be focused perfectly.
    Centering of the Electron Gun
    The centering is done by automatic procedure. It is always necessary to perform this auto centering
    after high voltage value changes inside a specified range with the item High Voltage on the Pad panel.
    1. Insert the centering specimen.
    2. Set up the recommended working conditions.
    3. Use the Adjustment >>> Auto Gun Centering function in the Electron Beam panel.
    Automatic Centering
    The automatic procedure of column centering is very fast and suitable for low magnifications. For very
    precise work at high magnifications manual centering is more suitable (see chapter 4.1.2.3).
    1. Insert the centering specimen.
    2. Set up the recommended working conditions and focus the image in the RESOLUTION mode.
    3. Use the Adjustment >>> Auto Column Centering function in the Electron Beam panel.
    Manual Centering
    1. Insert the centering specimen.
    2. Set up the recommended working conditions and focus the image in the RESOLUTION mode.
    Note: Manual centering is for experienced users and it is necessary to perform it individually
    for each from four HV indexes.
    3.Use the Adjustment >>> Manual Column Centering function in the Electron Beam panel. Press
    Next>> button. The image starts wobbling, that is, periodically changing the working distance.
    4. Minimize the image movement by changing the OBJ Centering (select in the Pad panel) using the
    trackball and F11 and F12 keys. Press the Finish button.
    It might also be necessary to have FIELD or DEPTH modes precisely centered if working on higher
    magnifications. If needed proceed with the following steps:
    5. Switch to the FIELD mode. Set the same magnification for the modes RESOLUTION and FIELD.
    Use the Adjustment >>> Manual Column Centering function in the Electron Beam panel. Press
    Next>> button. Minimize the image movement by changing the IML Centering (select in the Pad
    panel) using the trackball and the F11 and F12 keys. Press Next>> button. Adjust the OBJ Centering
    so that the image is the same as in the RESOLUTION mode (use the trackball and the F11 and F12
    keys). Press the Finish button.
    6. Switch to the DEPTH mode. Use again the procedure with Manual Column Centering and OBJ
    Centering (steps 3. and 4.).
    7. Switch to the WIDE FIELD mode and set the same magnification for the modes RESOLUTION and
    WIDE FIELD. Use the Adjustment >>> Manual Column Centering function in the Electron Beam
    panel. Press Next>> button. Adjust the OBJ Centering so that the image is the same as in the
    RESOLUTION mode (use the trackball and the F11 and F12 keys). Press the Finish button.
    Note: RESOLUTION mode is the basic and most common displaying mode. The procedure described
    above is sufficient for work in the RESOLUTION mode on high magnifications.
    It might also be necessary to have FIELD or DEPTH modes precisely centered if working on higher
    magnifications. If needed proceed with the following steps:
    1. Switch to the DEPTH mode and centre this mode as usual.
    2. Switch to the FIELD mode and only set the same field of view by changing the OBJ Centering.
    3. Switch to the mode WIDE FIELD mode and centre this mode as usual.
    Note: If you want to align field of view for the DEPTH mode exactly with the RESOLUTION mode (for
    magnifications > ~10000), set the same magnification in the RESOLUTION and DEPTH modes and
    then set the same field of view by means of changing the IML Centering parameter.
    WARNING: This procedure has a strong influence on the other centering parameters and thus is
    recommended only for experienced users.

    You might also like