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    Introduction To Coulter Counter Method

    Uploaded by

    Sachin Shinde

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Introduction To Coulter Counter Method

    Uploaded by

    Sachin Shinde
    11 views7 pages

    Original Title

    Introduction to Coulter Counter Method

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    Copyright:

    © All Rights Reserved
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    Download as pdf or txt
    11 views7 pages

    Introduction To Coulter Counter Method

    Uploaded by

    Sachin Shinde

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 7

    Introduction to

    Coulter Counter
    Method
    The Coulter Counter is a powerful instrument used to measure the size
    and count of particles suspended in a liquid. It is a widely used
    technique in various fields, including biology, medicine, and materials
    science.
    Principle of Coulter Counter

    1 Electrical Sensing 2 Electrical 3 Particle Size


    Zone Impedance Measurement
    The Coulter Counter As a particle passes The magnitude of the
    works on the principle of through the aperture, it pulse is proportional to
    electrical sensing, where displaces a volume of the volume of the
    particles are detected as the surrounding particle, allowing the
    they pass through a electrolyte, causing a size of the particle to be
    small aperture. change in the electrical determined.
    impedance, which is
    detected and converted
    into a pulse.
    Working of the Coulter Counter
    1 Sample Preparation
    The sample is suspended in an electrolyte solution and introduced into the
    sample chamber of the Coulter Counter.

    2 Particle Detection
    The sample is drawn through the small aperture, where each particle
    passing through causes a change in the electrical impedance, generating a
    pulse.

    3 Data Analysis
    The pulses are amplified, counted, and analyzed to determine the size
    distribution and concentration of the particles in the sample.
    Diagram of a Coulter Counter
    Sample Chamber Aperture Electrodes
    The sample is introduced into The particles pass through a The electrodes are used to
    the chamber, where it is small aperture, causing create an electrical field
    suspended in an electrolyte changes in the electrical across the aperture, allowing
    solution. impedance that are detected the changes in impedance to
    and analyzed. be measured.
    Advantages of the Coulter Counter
    Method
    Accuracy Automation
    The Coulter Counter provides highly The process is automated, allowing for rapid
    accurate measurements of particle size and and efficient analysis of samples, reducing
    concentration, making it a reliable tool for the time and effort required.
    various applications.

    Wide Range Minimal Sample


    The Coulter Counter can measure a wide Only a small sample volume is required for
    range of particle sizes, from 0.4 to 1200 analysis, making the Coulter Counter a
    micrometers, making it versatile for diverse cost-effective and efficient choice.
    applications.
    Disadvantages of the Coulter Counter
    Method

    1 Sensitivity to Contamination 2 Limited to Conductive Samples


    The Coulter Counter is sensitive to the The Coulter Counter requires the sample
    presence of contaminants in the sample, to be suspended in an electrolyte
    which can affect the accuracy of the solution, limiting its use to conductive
    measurements. samples.

    3 Potential Clogging 4 Maintenance Requirements


    The small aperture of the Coulter Counter The Coulter Counter requires regular
    can become clogged by larger particles, maintenance and calibration to ensure
    leading to inaccurate results. accurate and reliable results.
    Applications and Advantages of the
    Coulter Counter

    Biomedical Materials Environmental Industrial


    Widely used in Science Utilized to study Used for quality
    hematology, Applied in particle water quality, control and process
    immunology, and analysis for industries sediment analysis, monitoring in
    microbiology to like pharmaceuticals, and airborne manufacturing
    analyze cell sizes and cosmetics, and particulate matter. industries.
    concentrations. ceramics.

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