Syllanus of 6th Sem

C. U.
SHAH UNIVERSITY
FACULTY OF SCIENCES
DEPARTMENT OF LIFE SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: Medical Microbiology (Theory)
SUBJECT CODE: 4SC06MEM1
Teaching & Evaluation Scheme:-
Teaching hours/week Credit Evaluation Scheme/semester
Theory Practical
Sessional University Total
Th Tu Pr Total Internal
Exam Exam University Marks
Marks Hrs Marks Hrs Pr TW
4 2 0 4 5 30 1 70 3 - - 100
Objectives:- The objective of this course is that the students can learn about basics of
Medical Microbiology
Prerequisites:- Basic knowledge of Biological Sciences.
Course outline:-
Sr. Course Contents Hours
No.
1 Normal microflora of the human body and host pathogen 08
interaction: Normal microflora of the human body: Importance of
normal microflora, normal microflora of skin, throat, gastrointestinal
tract, urogenital tract
Host pathogen interaction: Definitions - Infection, Invasion, Pathogen,
Pathogenicity, Virulence, Toxigenicity, Carriers and their types,
Opportunistic infections, Nosocomial infections. Transmission of
infection, Pathophysiologic effects of LPS
2 Sample collection, transport and diagnosis: 05
Collection, transport and culturing of clinical samples, principles of
different diagnostic tests (ELISA, Immunofluorescence, Agglutination
based tests,Complement fixation, PCR, DNA probes).
3 Bacterial diseases: 15
List of diseases of various organ systems and their causative agents.
The following diseases in detail with Symptoms, mode of transmission,
prophylaxis and control
Respiratory Diseases: Streptococcus pyogenes, Haemophilus influenzae,
Mycobacterium tuberculosis Gastrointestinal Diseases: Escherichia coli,
Salmonella typhi, Vibrio cholerae, Helicobacter pylori Others:
Staphylococcus aureus, Bacillus anthracis, Clostridium tetani, Treponema
pallidum, Clostridium difficie
Syllabus of B.Sc. Sem-VI (Microbiology) WEF June 2016

C. U. SHAH UNIVERSITY
4 Viral diseases: 14
List of diseases of various organ systems and their causative agents. The
following diseases in detail with Symptoms, mode of transmission,
prophylaxis and control Polio, Herpes, Hepatitis, Rabies, Dengue, AIDS,
Influenza with brief description of swine flu, Ebola, Chikungunya,
Japanese Encephalitis
5 Protozoan diseases: 05
List of diseases of various organ systems and their causative agents.
The following diseases in detail with Symptoms, mode of transmission,
prophylaxis and control Malaria, Kala-azar
6 Fungal diseases: 06
Brief description of each of the following types of mycoses and one
representative disease to be studied with respect to transmission,
symptoms and prevention, Cutaneous mycoses: Tinea pedis (Athlete’s
foot) Systemic mycoses: Histoplasmosis, Opportunistic mycoses:
Candidiasis
7 Antimicrobial agents: General characteristics and mode of action: 08
Antibacterial agents: Five modes of action with one example each:
Inhibitor of nucleic acid synthesis; Inhibitor of cell wall synthesis;
Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism Antifungal agents: Mechanism of action of
Amphotericin B, Griseofulvin. Antiviral agents: Mechanism of action
of Amantadine, Acyclovir, Azidothymidine. Antibiotic resistance,
MDR, XDR, MRSA, NDM-1
Total Hours 61
Learning Outcomes:- The students are expected to

 Identification of various disesases and their symptoms.
 Mechanism of antimicrobial agents.
Books Recommended:-
1. Ananthanarayan R. and Paniker C.K.J. (2009) Textbook of Microbiology. 8th
edition, University Press Publication
2. Brooks G.F., Carroll K.C., Butel J.S., Morse S.A. and Mietzner, T.A. (2013)
Jawetz, Melnick and Adelberg’s Medical Microbiology. 26th edition. McGraw Hill
Publication
3. Goering R., Dockrell H., Zuckerman M. and Wakelin D. (2007) Mims’ Medical
Microbiology. 4th edition. Elsevier
4. Willey JM, Sherwood LM, and Woolverton CJ. (2013) Prescott, Harley and
Klein’s Microbiology.9th edition. McGraw Hill Higher Education
5. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of
Microorganisms.14th edition. Pearson International Edition
Syllabus of B.Sc. Sem-VI (Microbiology) WEF June 2016

PRACTICALS
Sr. No. Course Contents

1 Identify bacteria (any three of E. coli, Salmonella, Pseudomonas,
Staphylococcus, Bacillus
cillus) using laboratory strains on the basis of cultural,
cul
morphological and biochemical characteristics: IMViC, TSI, nitrate
reduction, urease prodduction and catalase tests
2 Study of composition and use of important differential media for
identification of bacteria:
eria: EMB Agar, McConkey agar, Mannitol salt agar,
Deoxycholate citrate aagar, TCBS
3 Study of bacterial florra of skin by swab method
4 Perform antibacterial sensitivity by Kirby-Bauer method
5 Determination of minnimal inhibitory concentration (MIC) of an antib biotic.
6 Study symptoms of tthe diseases with the help of photographs: Poolio, anthrax,
herpes, chicken pox, x, HPV warts, AIDS (candidiasis), dermatom mycoses (ring
worms)
7 Study of various stageses of malarial parasite in RBCs using permanent
anent
mounts.
Syllabi of B.Sc. Sem

Sem-VI (Microbiology) WEF June 2016
FACULTY OF SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: Recombinant DNA Technology (Theory)
SUBJECT CODE: 4SC06RDT1
Teaching & Evaluation Scheme:
Scheme:-
Theory Practical
4 0 4 8 6 30 1 70 3 30 - 70 200
Objectives:- The objective of this course is that the students can learn about basics of r-DNA
technology.
Prerequisites:- Basic knowledge of Biological Sciences and genetic material.
Course outline:-
Sr. Course Contents Hours
No.
1 Introduction to Genetic En
ngineering:
Milestones in genetic engineeering and biotechnology. 2
2 Molecular Cloning- Tools and Strategies :

Cloning Tools; Restriction modification systems: Types I, II and III. Modde of
action, nomenclature, appliplications of Type II restriction enzymes in geenetic
engineering
DNA modifying enzymes and their applications: DNA polymerases.
Terminal deoxynucleotidyl tr transferase, kinases and phosphatases, and DNA
ligases 20
Cloning Vectors: Definition and Properties Plasmid vectors: pBR and pUC
series Bacteriophage lambd bda and M13 based vectors Cosmids, BACs,
YACs Use of linkers and adaptors
adapt
Expression vectors: E.coli lac and T7 promoter-based vectors, yeast YIp,
YEp and YCp vectors, Baculovirus based vectors, mammalian SV40-based
expression vectors
3 Methods in Molecular Cloning:
ning:
Transformation of DNA: Chemical
Ch method, Electroporation,
Gene delivery: Microinjection,
tion, electroporation, biolistic method (gene gun),
liposome and viral- mediated delivery, Agrobacterium - mediated delivery 16
DNA, RNA and Protein annalysis: Agarose gel electrophoresis, Southern -
and Northern - blotting technniques, dot blot, DNA microarray analysis, SDS-
PAGE and Western blotting.ng.
4 DNA Amplification and DNA sequencing:
PCR: Basics of PCR, RT-PC CR, Real-Time PCR
10
Sanger’s method of DNA Sequencing:
Se traditional and automated sequencing
Primer walking and shotgun sequencing

5 Construction and Screenin ng of Genomic and cDNA libraries:
Genomic and cDNA libraries:
es: Preparation and uses, Screening of libraries: Coolony 6
hybridization and colony PCR, Chromosome walking and chromosome jumping ing
6 Applications of Recombinaant DNA Technology:
Products of recombinant DNA technology: Products of human therapeutic interest -
insulin, hGH, antisense mol
olecules. Bt transgenic - cotton, brinjal, Gene therap
erapy,
6
recombinant vaccines, protein
ein engineering and site directed mutagensis
Total Hours 60

 Handeling the molecular tools and genomic library.
 r-DNA
DNA application in transgenic mechanism.
Books Recommended:-
1. Brown TA. (2010). Gene Clo
Cloning and DNA Analysis. 6th edition. Black
kwell
Publishing, Oxford, U.K.
2. Clark DP and Pazdernik NJ. (2009). Biotechnology: Applying the Genetic

Revolution. Elsevier Acadeemic Press, USA
3. Primrose SB and Twyman

an RM. (2006). Principles of Gene Manipulatioon and
Genomics, 7th edition. Blackwell Publishing, Oxford, U.K.
4. Sambrook J and Russell D

D. (2001). Molecular Cloning-A Laboratory Manual.
Ma 3rd
edition. Cold Spring Harbor
or Laboratory Press
5. Wiley JM, Sherwood LM

M and Woolverton CJ. (2008). Prescott, Harley and
Klein’s Microbiology. McGraw Hill Higher Education
6. Brown TA. (2007). Genomes

Genomes-3. Garland Science Publishers
7. Primrose SB and Twyman

an RM. (2008). Genomics: Applications in human
hu biology.
Blackwell Publishing, Oxford, U.K.

PRACTICALS
Sr. No. Course Contents

1 Preparation of competent
etent cells for transformation
2 Demonstration of Bacterial Transformation and calculation of tra transformation
efficiency.
3 Digestion of DNA using
sing restriction enzymes and analysis by agarosee gel
electrophoresis
4 Ligation of DNA frag gments
5 Cloning of DNA inserrt and Blue white screening of recombinants.
6 Interpretation of sequenci
sequencing gel electropherograms
7 Designing of primers for DNA amplification
8 Amplification of DNA by PCR
9 Demonstration of Souuthern blotting

FACULTY OF SCIENCES
COURSE: B.Sc. SEMESTER: VI (All Sciences)
SUBJECT NAME: Professional Etiquettes-II
Etiquettes
SUBJECT CODE: 4SC06PRE2
Scheme:-
Theory Practical
2 2 0 4 3 30 1 70 3 - - - 100
Objectives:
 To define before the students professional behavior and suggest standards forappearance,
actions, and attitude in a business environment.
 To explain them different communication styles and how to adjust to each.
 Review the essentials off online and offline business networking.
 Develop an action plan to improve personal professionalism.
Prerequisites:
 Students should have basic knowledge of English Language and grammar.
 Students should have ability to speak and write correct sentences in their day to day
language.
 Students should be familiar with correct usage of language.
Course outline:-
Minimum Number of
Unit Content In Details Including Its Sub Topics Hours
No.
Theory Practical Total
Section: A: Career Advancement Program 2
Positive Attitude
Introduction
01 Importance of positive attitude 03 02 05
Ways to develop positive attitude
External and internal factors in bulging positive attitude
Self Esteem
Definition
Theory of Maslow
02 03 03 06
Advantages of High Self Esteem
Causes of Low Self Esteem
How to Improve Self Esteem
Leadership
Who is Leader?
Characteristics of Leader
03 04 04 08
Types of Leader
Importance of Leadership in Professional life
Case study of established leaders.

Soft Skills
Introduction to soft skills
Importance of soft skills
Intelligence quotient, Emotional quotient, Spiritual quotient
Classification of soft skills:
04 Thinking Skills:: Self Awareness, Problem-Solving,
Problem Decision 05 05 10
Making, Critical Thinking, Creative Thinking
Social Skills: Interpersonalal Relationships, Effective
Communication, Empathy
Emotional Skills:Managing
Managing Feelings/emotions, Stress
Management
Goal Setting
Introduction
Importance
5 D’s of Goal Setting: Direction, Dedication, Determination,
05 04 04 08
Discipline,
Deadline
Steps of Goal
Action Plan
Notice, Agendas of Meeting, Minutes of Meeting
Preparing notice
06 03 02 05
Preparing a list of agendas for meeting
Drafting minutes of conducted meeting
Section-B: Literature
07 La Belle Dame Sans Merci – John Keats 08 10 18
Byzantium – W. B. Yeats

 Prepare participants to handle a variety of social and business situations: networking
events, business meetings, business meals, and manymore.
Books Recommended:-
1. Personality Development and Soft Skills,
Skills,Mitra Barun,Oxford
,Oxford University Press.
2. Managing Soft Skills for Personality Development,B.N.
Development, Ghosh, Tata McGraw
McGraw-Hill.
3. You Can Win,Shiv Khera.
4. The Monk Who Sold His Ferrari,
Ferrari,Robin Sharma, Harper Collins.
5. Effective Personal Communication Skills for Public Relations,
Relations,Green
Green Andy
Andy, Kogan age
Limited.
6. William Butler Yeats: The Byzantium Poems, The Merrill literary casebook series
series, Ed.
Richard J. Finneran,, Merrill Publishing Company, Chicago.
7. JOHN KEATS: Selected Poems Poems, Edited and with ith an Introduction and Notes,John
Notes,
Barnard,, Penguin Books New Delhi.
8. Selected College Poems, Ambika Sengupta
Sengupta,, Orient Longman Private Limited, New
Delhi.

FACULTY OF SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: DSE III (INSTRUMENTATION AND BIOTECHN
NIQUES)
SUBJECT CODE: 4SC06IAB
4SC06IAB1
Scheme:-
Theory Practical
4 0 4 8 6 30 1 70 3 30 - 70 200
Instruments and biotechniques.
Sr. No. Course contents Teaching
Hours
1 Microscopy:
icroscopy: 10
Bright field and dark field
ld microscopy, Fluorescence Microscopy,, Phase
contrast Microscopy,, Confocal Microscopy, Electron
ctron
Microscopy(Scanning andd Transmission Electron Microscopy) and
Micrometry.
2 Chromatography: Principles
ples and applications of paper cchromatographyy 14
(including Descending annd 2-D), Thin layer chromatography. Columnn
packing and fractionn collect
collection. Gel filtration chromatography, ion--
exchange chromatographyy and aff
affinity chromatography, GLC,HPLC.
3 Electrophoresis: 14
Principle and applications
ons of nat
native polyacrylamide gel electrophoresis,
horesis, SDS
SDS-
polyacrylamide gel electroph
ctrophoresis, 2Dgelelectrophoresis, Isoelect
ctric
focusing, Zymo gram prepparation and Agarose gel electrophoresis
4 Spectro photometry:
y: 10
Principle and use of studyy of absorption spectra of biomolecules. Analy
ysis
of biomolecules using UVV and visible range. Colorimetry and turbidometr
metry.
5 Centrifugation:
ion: 12
Preparative and a n a l y t i c a l centrifugation, fixed angle and s w i n g i n g
bucket rotors.RCF
.RCF and sedsedimentation coefficient, differential centrifugation,
gation,

density gradient centrifugattion and ultra centrifugation.
Total Hours 60

 Handling the Instruments
Instruments.
Books Recommended:-
1. Wilson K and Wallker J.(2010).Principles and Techniques off Biochemistry

Bioch and
.7thEd. ,Cambridge University Press.
Molecular Biology.7
2. Nelson DL and d Cox MM. (2008). Lehninger Principles off Bioc

Biochemistry,
th
5 Ed., W.H. Freem
man and Company.
3. Willey MJ, Sherwo

erwood LM &WoolvertonC J.. (2013). Prescott, Harley and
Klein’s Microbiologgy.9thEd.,McGraw Hill.
4. Karp G.(2010) 0) Cell aand Molecular Biology: Concepts and Exper

xperiments. 6th
edition. John Wiley
iley & So
Sons. Inc.
5. De Robertis
obertis EDP and DeRobertis EMF. (2006). Cell and Molecular
lecular B
Biology.
8th edition. Lipincott
ncott Wi
Williams and Wilkins, Philadelphia.
6. CooperG.M. and d HaHausman R.E.(2009). The Cell:: A Molecular

th
Approach.5 Edition.
tion. ASM Press & Sunderland, Washington
on D.C Sinauer
S
Associates, MA.
7. Nigam A and Ayy yyagariA.2007. Lab Manual in Biochemistry,, Immu

Immunology
and Biotechnology .T
.Tata McGraw Hill.

PRACTICALS
S.No Experiment
1 Study of fluorescent m
micrographs to visualize bacterial cells.
2 Ray diagrams of phasee contrast microscopy and Electron microscopy.

copy.
3 Separation of mixtures
ures by paper/thin layer chromatography.
4 Demonstration of column
umn packing
pac in any form of column chromatograph
matography.
5 Separation of protein m
mixtures by any form of chromatography
6 Separation of protein m
mixtures by Polyacrylamide Gel Electrophoresis
oresis (PAGE).
7 Determination of λmaxx for anun known sample and calculation of extincti

inction
coefficient.
8 Separation of componen
ents of a given mixture using a laboratoryy scale centrifuge.
9 Understanding density
ity gradient centrifugation with the help of pictures.
ures.

FACULTY OF SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: DSE III (ADVANCES IN MICROBIOLOGY)
Y)
SUBJECT CODE: 4SC06AIM1
4SC06AIM
Scheme:-
Theory Practical
4 0 4 8 6 30 1 70 3 30 - 70 200
Advance in microbiology.

Sr. Course contents Teaching
No.
Hours
1 Evolution off Microbial

Microbi Genomes:
omes: 15
Salient features of sequenced
uenced microbial genomes, core genome pool, flexxible
genome pool and cooncept of pan genome, Horizontal gene g
transfer(HGT),Evolutionn of bbacterial virulence-Genomic islan
islands,
Pathogenicity islands(PAI)a
AI)and their characteristics
2 Meta genomics cs : 15
Brief history and developme pment of metagenomics , Understanding ng bacterial
diversity using meta gen genomics approach, Prospectingg genes of
biotechnological importance
rtance using meta genomics
Basic knowledge
wledge of viral meta genome, meta transcriptomics, ics, me
meta
proteomics and meta bolom mics.
3 Molecular Basis of Host-Mic Microbe Interactions:ns: 15
Epiphytic fitness
tness and its mechanism in plant pathogens, Hyper sensitiveensitive
response(HR)to plant pathhogens and its mechanism, Type three secretion etion
systems (TTSS) of plannt and animal pathogens, Biofilms: typess of
microorganisms, molecular
lecular aaspects and significance in environment ,health
ealth
care, virulence and anti miicrobial resistance
4 Systems andnd Synthetic BioBiology:
ogy: 15
Networking in biological
ological systems, Quorum sensing in bacteria, Co--
ordinated regulation of baccterial virulence factors, Basics of synthesis off
polio virus in laboratory,, Future implications of synthetic biologyy with
respect to bacteria and viruses
Total Hours 60


 Understanding bacterial ddiversity using meta genomics approach.
Books Recommended:-
1. Fraser CM,, Read TD and Nelson
Nel KE .Microbial Genomes,2004, Humana
mana Press.
2. Miller RV and Day MJJ. Microbial Evolution-Gene establishment, survvival and

exchange, 2004, ASM Press.
3. Bull AT. Microbial Divversity and Bioprospecting, 2004, ASM Press.
4. Sangdun C. Introductioon to Systems Biology, 2007, Humana Press.

PRACTICALS
S.No Experiment
1 Extraction of meta genom
genomic DNA from soil
2 Understand the impediiments in extracting meta genomic DNA from soil
3 PCR amplification of m
meta genomic DNA using universal 16s ribosom
mal gene
primers
4 Case study to understa
erstand how the polio virus genome was synthesized
nthesized in the
laboratory
5 Case study to understa
erstand how networking of metabolic path wayss in bacteria takes
place

FACULTY OF SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: DSE III (BIOSAFETYAND INTELLECTUAL PROPPERTY
RIGHTS)
SUBJECT CODE: 4SC06BSI
BSI1
Scheme:-
Theory Practical
4 0 4 8 6 30 1 70 3 30 - 70 200
Objectives:- The objective of this course is that the students can learn about bas
basics of
Biosafety and IPR.
Prerequisites: - Basic knowledge

ledge of Biological Sciences
Sciences.

No. Hours
1 Bio safety: Introduction;; bio
b Safety Issues In biotechnology; Biological gical 8
Safety Cabinets & their tytypes; Primary Containment for Biohazards; Bioo
safety Levels of Specific Microor
Microorganisms
2 Bio safety Guidelines: Biio safety guidelines and regulations(National onal aand 12
International); GMOs-Concerns
Concerns and Challenges; Role of Institutionall B Bio
safety Committees(IBSC),
mittees(IBSC),RCGM, GEAC etc. for GMO applications inn food f
and agriculture; Environme
nvironmental release of GMOs; Risk Analysis; sis; Risk
Assessment; Risk man anagement and communication; Overview erview of
International Agreements--Cartagena Protocol.
3 AERB/RSD/RES guidelin ines for using radio isotopes in laboratoriess and 4
precautions.
4 Introduction to Intellecttual Property: Patents, Types, Trademarks, rks, 12
Copyright & Related Rig ghts, Industrial Design and Rights, ts, Traditional
Knowledge, Geographicalical In
Indications-importance of IPR– patentable an nd
non patentable –patenting ng life–lega
life lprotection
on of biotechnological
linventions– World Intellectual PProperty Rights Organization(WIPO).
5 Grant of Patent and Patent enting Authorities: Types of patent applications:
ions: 12
Ordinary, PCT, Convent entional, Divisional
onal and Patent of Addition; An
introduction too Patent Filing Procedures;
Proce Patent licensing and agreement;
ent;
Patent infringement mean aning, scope, litigation, case studies, Rights
ghts and
Duties of patent owner.
6 Agreements
ents and Treaties: GA GATT, TRIPS Agreements; Role of Madrrid 12

Agreement; Hague Agree Agreement; WIPO Treaties; Budapest Treaty y on
international recognition oof the deposit of microorganisms; UPOV & Brene
ene
conventions; Patent Co-operat
peration Treaty(PCT); Indian Patent Act1970
70 &
recent amendments.
Total Hours 60
Learning Outcomes:-
At the end of the course the student would have sufficient knowledge of IPR.
Books Recommended:
1. Bare Act,2007.
07. In
Indian Patent Act 1970Acts
Acts & Rules, Universal Law
Publishing Co. Pvt.
vt. Ltd., NewDelhi.
2. Kankanala C(20077). Genetic Patent Law & Strategy, 1stEdition,

on, Man
Manupatra
Information Solutioon Pvt. Ltd. New Delhi.
3. Mittal , D.P.(1999).Indian
.Indian Patents Law,
L Taxmann , Allied
lied Services(p)Ltd.
Services(
4. Singh KK(2015).B .Biotechnology and Intelectual Property Rights:

ts: Legal aand
Social Impliocationss, Springer India.
5. GoelD & Prashar

har S
S(2013).IPR ,Bio safety and Bioethics. Pearson.
n.
6. Senthil Kumarr Sa
Sadha sivam and Mohammed Jaabir,M.S.. 2008.IPR,
20 Bio
echnology Management. Jasen Publications,
safety and biotechno Tir
s, Tiruchirappalli,
India.

PRACTICALS
S.No Experiment
1 Study of components and design of a BSL-IIIlaboratory
2 Filing applicationss for approval from bio safety committee
3 Filing primary applications
lications for patents
4 Study of steps of a patent
patenting process
5 A case study

FACULTY OF SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: DSE IV (PLANT PATHOLOGY)
SUBJECT CODE: 4SC06PPY
PPY1
Scheme:-
Theory Practical
4 0 4 8 6 30 1 70 3 30 - 70 200
Objectives:- The objective of this course is that the students can learn about bas
basics of Plant
pathology.
Prerequisites: - Basic knowledge

ledge of Biological Sciences
Sciences.

No. Hours
1 Introduction
duction and History of plant
p pathology: 12
Concept of plant diseaseease-definitions of disease, disease cycle cle &
pathogenicity, symptoms associa
associated with microbial plant diseases, types
pes of
plant pathogens ,economic
mic losses and social impact of plant diseases.
Significant land marks in tthe field of plant pathology-Contributions

tributions of Ant
Anton
De Bary, Millardet, Burrill,
rrill, E.Smith,
E. Adolph Mayer, Ivanowski, i, Diener,
Stakman, H.H. Floor, VanDer Plank, molecular Koch’s
ch’s
postulates.Contributions off em
eminent Indian plant pathologists.
2 Stages in development
ment of a di disease: 6
Infection, invasion,
asion, col
colonization, dissemination of pathogenss and
perennation.
3 Plant disease epidemiology:
ogy: 10
Concepts of monocyclic,
clic, poly
p cyclic and polyetic diseases, disease trianglle &
disease pyramid, forecast
forecasting of plant diseases and its relevance ance in Indian
Ind
context.
4 Host Pathogen
thogen Interaction Microbial Pathogenicity: Virulence lence factors of 20
pathogens: enzymes, toxins(host
s(host sspecific and nonspecific)
Growth regulators, virulen ence factors in viruses (replicase, coat prottein,
silencing suppressors) in dis
isease development.
Effects of pathogens onn hhost physiological processes (photosynthesi

nthesis,
respiration, cell membrane rane mmermeability, translocation of water
er aand
nutrients, plant growthh and reproduction).
Genetics of Plant Diseases: Concept of resistance(R)gene and

nd a

virulence(avr) gene; genee ffor gene hypothesis, types of plant resistance:
istance: true
tr
resistance–horizontal
zontal & verti
vertical, apparent resistance.
Defense Mechanismss in Plants: Concepts of constitutive defen fense

mechanisms in plants,, induc
inducible structural defenses (histological- corkla
corklayer,
abscission layer, tylosses, gums), inducible biochemical defenses efenses
[hypersensitive response se (HR),systemic acquired resistance(SAR),ph ,phyto
alexins, pathogenesis relateed(PR)proteins, planti bodies, phenolics, quinoones,
oxidative bursts].
5 Control off Plant Diseases: 12
Principles & practices involved
volved in the management of plant diseasess by
different methods,viz. regula
regulatory quarantine, crop certification, avoidance
nce
of pathogen, use of pathoogen free propagative material cultural-host host
eradication, crop rotation, sanitat
sanitation, polyethylene traps and mulches
lches
chemical-protectants
ants and systemic fungicides, antibiotics, resistance off
pathogens to chemicals.cals. Biological-suppressive soils, antagonistic ic
microbes-bacteria and funggi, trapplants.
Genetic engineering of dise

sease resistant plants-with plant derived
ed genes and
pathogen derived genes
Total Hours 60
Learning Outcomes:-
At the end of the course the student would have sufficient knowledge of Plant Pathology.
Pathology
Books Recommended:
1. Agrios GN.(2006).Plant
.(2006).Plant Pat
Pathology.5thedition.Academic press,SanDieg
go.
2. Lucas JA.(1998).
98). Plant Pathol
Pathology and Plant Pathogens. 3rd editio
ion. Blackwell
Science, Oxford.
3. Mehrotra RS.(1994).Plant
).Plant Pa
Pathology. Tata McGraw-Hill Limited.
4. Rangaswami G.(2005).Diseases
.(2005).Diseases of Crop Plan
Plants in India. 4th edition.. Prentice
Hall of India Pvt. Ltd., New
NewDelhi.
5. Singh RS.(1998).Plant
).Plant Diseases Management. 7th edition. Oxford
rd & IBH,
New Delhi

PRACTICALS
Experiment
Demonstration of Koch’s poostulates in fungal, bacterial and viral plant path

thogens.
Study of important diseases
seases of crop plants by cutting sections off infected plant
material-Albugo, Puccinia,Us
Ustilago,Fusarium,Colletotrichum

FACULTY OF SCIENCES
COURSE: B.Sc.
SEMESTER: VI
SUBJECT NAME: DSE IV ((MICROBES IN SUSTAINABLE
AGRICULTURE AND DEEVELOPMENT)
SUBJECT CODE: 4SC06MAD
MAD1
Scheme:-
Theory Practical
4 0 4 8 6 30 1 70 3 30 - 70 200
Microbes in sustainable agriculture and development.

No. Hours
1 Soil Microbiology y 8
Soil as Microbial
ial Habitat, Soil proprofile and properties, Soil formation,
Diversity and distribution o of microorganisms in soil
2 Mineralization of Organiic & Inorganic Matter in Soil 8
Mineralization of cellulose,
se, hemicelluloses, lignocelluloses, lignin and
humus, phosphate, nitrate,
trate, silica, potassium
3 Microbial Activity in Soiil and Green House Gases ses 5
Carbon dioxide, methanee ,nitr
,nitrous oxide ,nitric oxide–production and control
4 Microbial Control of Soil oil Borne Plant Pathogens 8
Bio control mechanisms ms and wa ways, Microorganisms used as bio con ntrol
agents against Microbial plant
lant pathogens, Insects, Weeds
5 Bio fertilization, Phytostiimulation, Bioinsecticides 15
Plant growth prommoting bateria,biofertilizers symbiotic
(Bradyrhizobium, Rhizobiium, Frankia),Non Symbiotic(Azospirillum,
Azotobacter, Mycorrhizae,, MHBs, Phosphate solubilizers, algae), Novel
combination of microbes
crobes as biof
biofertilizers, PGPRs
6 Secondary Agriculturee B Biotechnology y 16
Biotech feed, Silage, Biio manure, bio gas, bio fuels–advantagess and
processing parameters
GM cropsps
Advantages, social al and en environmental aspects, Bt crops, golden lden rice,
transgenic animals.
Total Hours 60

Learning Outcomes:-
At the end of the course the student would have sufficient knowledge of agriculture.
agriculture
Books Recommended:
1. Agrios GN.(2006).Pla
.(2006).Plant Pathology.5th edition. Academic press,
s, San Diego.
2. Singh RS.(1998).Plant
.Plant Diseases Mana
Management. 7th edition. Oxford
rd & IBH, New
Delhi.
3. GlickBR, Pasternak
akJJ, and PattenCL (2010) Molecular Biotec
echnology
4thedition,ASM
ASM Pres
Press.
4. Atlas RM and d Bar

Bartha R.(2000). Microbial Ecology: Fund damentals &
Applications. 4theditio
edition.Benjamin/Cummings Science Publishing,USA.
USA.
5. Maier RM, Pepperpper IL and Gerba CP. (2009). Environmental

vironmental
Microbiology. 2ndedit
edition, Academic Press.
6. Barton LL & NorNorthup DE(2011). Microbial Ecology. 1stedition,

stedition, Wiley
Blackwell,USA.
7. Campbell RE.(1 (1983).Microbial Ecology. Blackwell Scien

entific
Publication, Oxford,
ord, England.
8. CoyneMS.(2001).S
01).Soil Microbiology: An Exploratory Approac
oach. Delmar
Thomson Learning
g.
9. Altman A(1998).Agricul
.Agriculture Biotechnology, I st edition,
ition, Marcel decker Inc.
10. MahendraK. Rai(2 (2005). Hand Book of Microbial Biofertilizers,, The Haworth
Press, Inc. New Yo
ork.
11. Reddy, S.M. M. et.al.

et.al.(2002).Bioinoculants for Sustainable
le Agriculture and
Forestry, Scientific Pu
Publishers.
12. SaleemF andd Sha

Shakoori AR(2012) Development
ent of Bio insecticide,
insect Lap
Lambert Academic
ic Publishing Gmb HKG

PRACTICALS
S.No Experiment
1 Study soil profile
2 Study micro flora off different types
t of soils
3 Rhizobium as soil inocculants characteristics and field application
4 Azotobacter as soil inoculants characteristics
char and field application
5 Design and functioningg of a biogas plant
6 Isolation of cellulose ddegrading organisms


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Prerequisites:- Basic knowledge of Biological Sciences.

Syllabus of B.Sc. Sem-VI (Microbiology) WEF June 2016

Learning Outcomes:- The students are expected to

Syllabus of B.Sc. Sem-VI (Microbiology) WEF June 2016

Sr. No. Course Contents

Syllabi of B.Sc. Sem

Prerequisites:- Basic knowledge of Biological Sciences and genetic material.

2 Molecular Cloning- Tools and Strategies :

Syllabi of B.Sc. Sem

Learning Outcomes:- The students are expected to

2. Clark DP and Pazdernik NJ. (2009). Biotechnology: Applying the Genetic

3. Primrose SB and Twyman

4. Sambrook J and Russell D

5. Wiley JM, Sherwood LM

6. Brown TA. (2007). Genomes

7. Primrose SB and Twyman

Syllabi of B.Sc. Sem

Sr. No. Course Contents

Syllabi of B.Sc. Sem

Syllabi of B.Sc. Sem

Learning Outcomes:- The students are expected to

Syllabi of B.Sc. Sem

Prerequisites:- Basic knowledge of Biological Sciences.

Sr. No. Course contents Teaching

Syllabi of B.Sc. Sem

Learning Outcomes:- The students are expected to

1. Wilson K and Wallker J.(2010).Principles and Techniques off Biochemistry

2. Nelson DL and d Cox MM. (2008). Lehninger Principles off Bioc

3. Willey MJ, Sherwo

4. Karp G.(2010) 0) Cell aand Molecular Biology: Concepts and Exper

6. CooperG.M. and d HaHausman R.E.(2009). The Cell:: A Molecular

7. Nigam A and Ayy yyagariA.2007. Lab Manual in Biochemistry,, Immu

Syllabi of B.Sc. Sem

2 Ray diagrams of phasee contrast microscopy and Electron microscopy.

7 Determination of λmaxx for anun known sample and calculation of extincti

Syllabi of B.Sc. Sem

Prerequisites:- Basic knowledge of Biological Sciences.

1 Evolution off Microbial

Syllabi of B.Sc. Sem

Learning Outcomes:- The students are expected to

2. Miller RV and Day MJJ. Microbial Evolution-Gene establishment, survvival and

3. Bull AT. Microbial Divversity and Bioprospecting, 2004, ASM Press.

4. Sangdun C. Introductioon to Systems Biology, 2007, Humana Press.

Syllabi of B.Sc. Sem

Syllabi of B.Sc. Sem

Prerequisites: - Basic knowledge

Sr. Course contents Teaching

Syllabi of B.Sc. Sem

2. Kankanala C(20077). Genetic Patent Law & Strategy, 1stEdition,

4. Singh KK(2015).B .Biotechnology and Intelectual Property Rights:

5. GoelD & Prashar

Syllabi of B.Sc. Sem

Syllabi of B.Sc. Sem

Prerequisites: - Basic knowledge

Sr. Course contents Teaching

Significant land marks in tthe field of plant pathology-Contributions

Effects of pathogens onn hhost physiological processes (photosynthesi

Genetics of Plant Diseases: Concept of resistance(R)gene and

Syllabi of B.Sc. Sem