Evolution of Microscopes

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evolution of

MICROSCOPY
introducing

-the cryogen microscope


f

-Fluorescent staining technique


Cryogenic
Faster Analysis:
01 :Technological advancements have made Cryo-EM
data collection and analysis faster, allowing for
more efficient studies.

MICROSCOPE Cellular Biology


02 : Visualizing the organization of proteins and
organelles within cells provides insights into
cellular function.
Cryogenic electron microscopy , often called cryo-EM for short, is
a powerful imaging technique which allows scientists to see
biological molecules, like proteins and viruses, the cryo-EM flash
freezes samples in liquid nitrogen, preserving their natural state .
03 Understanding Diseases
:Cryo-EM can help us see how viruses work,
CryoEM and cryoET enable high-resolution, three-dimensional which can aid in developing vaccines and
data collection on samples that could not be used with other treatments.

techniques in the past


For the study of nanobubbles, namely, of lipid/polymer-
stabilized gas-containing nanostructures, which can be
triggered externally by ultrasound
fluorescent 01
live cell-imaging
Fluorescent staining can be done on either live
or fixed samples. This allows researchers to study

STAINING samples in real time .

can detect tiny amounts


Fluorescent staining can provide high-resolution 02 Fluorescent stains can detect even very small
quantities of a target molecule within a cell.
images with great specificity, allowing researchers
to study the interactions, and dynamics of different
cellular components in living or fixed samples.
Target Specific molecules
Once the cells are stained, scientists use a special
microscope called a fluorescence microscope. This 03 Fluorescent stains can be designed to bind
very specifically to particular molecules
microscope shines a light on the sample. within a cell, such as proteins, DNA, or lipids.
The goal of this study was to examine the effect of
epidermal growth factor (EGF) on sperm viability using
two fluorescent techniques .

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