6300 Janway

Jenway 6300
Spectrophotometer Service Manual
Main Index
Section 1 Introduction
Section 2 Quick Reference
Section 3 System Description
Section 4 Optical Description
Section 5 Electronic Description
Section 6 Software and Operation
Section 7 Diagnostics
Section 8 Maintenance
Section 9 Circuit Diagrams
Section 10 Assembly Diagrams
Section 11 Spare Parts List
Jenway 6300 Ser Man 1
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Section 1
Introduction
1.0 Index to Sections
1.1 About This Manual
1.2 Using This Manual
1.3 Warnings & Safe Practice
1.4 Standards & Certification
1.5 Ordering Spares
1.6 Returning Items
1.7 Contacting Jenway Limited

Introduction.
Section 1 – Introduction
1.5 Ordering Spares
1.6 Returning Items
Section 2 - Quick Reference

2.0 About ‘Quick Reference’
2.1 Specification
2.2 Main Sub-Assemblies
2.3 Power Supply Voltages
2.4 Signal Levels
2.5 Error Codes
2.6 Special Key Functions
2.7 Test Solutions
Section 3 - System Description

3.1 Background
3.2 Sub-Assemblies
3.3 Accessories
3.4 Outputs
Section 4 - Optical Description

4.1 Light source
4.2 Grating
4.3 Shutter and Filter
4.4 Signal Detector

Section 5 - Electronic Description
5.1 Power Supplies
5.2 Detector Circuit
5.3 Microprocessor and Display
Section 6 - Software and Operation

6.0 Warning
6.1 Start Up Routine
6.2 Photometrics
6.3 Concentration
Section 7 – Diagnostics
7.1 Diagnostics Mode
7.2 Shutter and Filter Control
7.3 Lamp Control
7.4 Zero Order Calibration.
Section 8 – Maintenance
8.1 Routine Maintenance
8.2 Dismantling
8.3 Optical Alignment
8.4 Energy Levels
8.5 Wavelength Calibration
8.6 A to D Calibration
8.7 D to A Calibration
8.8 Performance Verification

Section 9 - Circuit Diagrams
9.1 Supply Schematic 630 504
9.2 Power Supply Layout 630 504
9.3 Detector PCB Schematic 630 506
9.4 Detector PCB Layout 630 506
9.5 Microprocessor PCB Schematic 630 013
9.6 Microprocessor PCB Layout 630 013
Section 10 - Assembly Diagrams

10.1 6300 Final Assembly 630 503
10.2 6300 Lower Case Assembly 630 510
10.3 6300 Top Case Assembly 630 010
10.4 6300 Optics Assembly 630 508
10.5 6300 Rear Panel Assembly 630 012
Section 11 – Spare Parts List

11.01 Packed Instrument
11.02 Top Case Assembly
11.03 Microprocessor/display PCB
11.04 Lower Case Assembly
11.05 Lamp Housing Assembly
11.06 Monochromator Assembly
11.07 Detector PCB
11.08 Power Supply PCB
11.09 Rear Panel Assembly

This manual covers the service, maintenance, calibration and repair
of the Jenway Ltd model 6300 Spectrophotometer. (From serial
number 5000 upwards, for lower numbers please refer to 6300
Mk1 Manual)
This manual must be used in conjunction with the Instruction
Manual for this model, as many of the routine maintenance
procedures detailed in the Instruction Manual are not repeated in
this Service Manual.

This manual is only for the use of Engineers and Technicians who
have successfully completed a Jenway Ltd approved Service
Training course on the Model 6300 Spectrophotometer.
Updates to this manual will be circulated through the Jenway Ltd

TSI (Technical Service Information) systems and to other
registered users of this manual. Please complete the form at the rear
of this manual to register your copy for future updates.
In practice Section 2 - Quick Reference and Section 8 –

Maintenance, with the Diagrams in Section 9 and 10, will be most
frequently used, however it is good practice to read the complete
manual initially and review it again periodically.
To find the information required refer to the Main Index or Index

to Sections to identify the relevant Section/page number required.

Always disconnect the mains supply when any covers are removed
as there are voltages present inside the unit that pose the risk of
electric shock at levels that are hazardous to life!
Do not look directly at the light source or allow the light beam to
fall directly on the eyes, switch off or dim the lamp (as described in

the procedures) whenever possible and wear eye protection at ALL
times.
The lamp gets very hot when in use, always allow time for it to
cool down before removal. Always wear cotton gloves when
removing a faulty lamp and replacing with a new one.
Finger marks, dust and condensation can quickly destroy sensitive

and expensive optical components, always wear cotton gloves
when the optical bench is uncovered and handle any components
by their edges only. Never touch optical surfaces. Do not remove
optical covers unless the unit is in a clean, dust and condensation
free environment.
Many of the reagents, solutions and standards used for maintenance

and calibration are corrosive or hazardous, ensure all precautions
supplied with them are followed, where there is any doubt request a
MSDS (Material Safety Data Sheet) from the supplier.
This instrument can be used for analysing a broad range of

samples, do not handle them unless you are qualified to do so.
Ensure that the instrument has been correctly decontaminated
before working on it, specifically in areas where the instrument
may have been used for clinical, biological, corrosive or
radioactive samples.

No adjustments should be made to this instrument unless the test
and measurement equipment, signal source or filters to be used
have a current calibration certificate that is traceable to national or
international standards and that it is known that this test equipment
is currently performing to the certified standards. All solutions and
reagents should be fresh and within any stated shelf life with a
certificate of analysis.
1.5 Ordering Spares

When ordering spare parts as detailed in this manual please quote
the Part Number and Description. These items should be ordered
from the original supplier of the equipment or your local Jenway
Limited Distributor.

1.6 Returning Items
Should it be necessary to return any item for any reason then this
should be done through the original supplier of the equipment or
your local Jenway Limited Distributor.

Before contacting Jenway Limited please check our web pages for
any information or updates that may be helpful to you.
www.jenway.com
Emails should be sent to sales@jenway.com
Fax: +44 1371 821083
Phone: +44 1371 820122
Please note no items can be returned to (or will be accepted by)

Jenway Limited without a Returns Authorisation number (RA
number) and a completed Safety Clearance and Decontamination
certificate.

Section 2
Quick Reference
2.1 Specification
2.4 Signal Levels
2.5 Error Codes
2.7 Test Solutions

This section contains a selection of the key information that is
often forgotten or difficult to find when required. Use Quick
Reference as a memory jogger, but for more information check out
the references to the main sections on each point.
2.1 Specification
Also see Section 1.2 of the Instruction Manual.
Wavelength Range 320nm to 1000nm

Wavelength Resolution 1nm
Wavelength Accuracy +/-2nm
Spectral Bandwidth 8.0nm
Light Source Tungsten Halogen
Optics Single Beam
Transmittance Range; 0 to 199.9%
Resolution; 0.1%
Absorbance Range; -0.300A to 1.999A
Resolution; 0.001A
Concentration Range; -300 to 1999
Resolution; 0.1, 1
Units; ppm, mgl-1, gl-1, M, %, blank.
Factor 0 to 999.9 / 1000 to 9999
Photometric Accuracy +/-1%
Photometric Noise Less than 1%
Stray Light Less than 0.5%T
Photometric Stability Better than 1% per Hour (after warm up)
Readout Custom LCD
Outputs Analogue (0 to 1999mV) & RS232 Serial
Supply Voltages 115/230 V a.c.
Power Less than 50W
Dimensions 365 (w) x 272 (d) x 160 (h) mm
Weight 6Kg

Also see Section 11 – Spare Parts
630 010 Top Case Assembly – includes the following…
630 025 Keypad

630 013 Display PCB
630 510 Lower case Assembly – includes the following…
630 508 Optics Assembly

630 504 Power Supply PCB
630 506 Detector PCB
060 311 Cooling Fan
010 040 Torroidal Transformer
630 508 Optics Assembly – includes the following…
012 075 Tungsten Halogen lamp

032 005 12V Solenoid
630 516 IR Filter
630 012 Rear Panel Assembly – includes the following…
016 021 2A Fuse 20 x 5mm

017 050 Mains Switch
009 123 Mains Input Socket

Also see Section 9 - Circuit Diagrams
Before commencing more complex fault finding it is important to

check all the internally generated supply voltages are correct. The
following list is a useful guide to help quickly check these are
functioning correctly. Not all the points where these voltages can
be measured are given and where the voltage is stated as
unregulated variations may occur. In general regulated supplies
should vary by no more than +/-5% from their nominal value.
Tungsten Lamp Supply, 12V dc regulated and set by VR1,

measure at SK9 pin 5 with respect to SK9 pin 6 on the power
supply PCB and at the terminals on the lamp base with the lamp
fitted.
Solenoid and Fan Supplies, 12V dc regulated and pre-set,

measure at SK1 pin 1 with respect to SK1 pin 2 on the power
supply PCB and on the solenoid and fan terminals.
Digital Supply, 5V dc regulated and pre-set, measure at SK5 pin 1

with respect to SK5 pin 2 or on SK1 pin 1 with respect to SK1 pin
2 on the Display PCB.
Stepper Motor Drive, 30V dc unregulated, also acts as

unregulated supply for lamp, solenoid and fan supplies, measure
between Star1 and Star 2 on power supply PCB.
DAC Supply, +/- 10V dc supplies.

+10V dc generated on the RS232 Interface, IC101, on the Display
PCB, measure between pins 2 (positive) and 15 (0V) on IC101 on
the Display PCB.
-10V dc generated on the RS232 Interface, IC101, on the Display
PCB, measure between pins 8 (negative) and 15 (0V) on IC101 on
the Display PCB.

2.4 Signal Levels
Also see Section 7.2 - Shutter and Filter and 8.3 – Energy
Levels
All analogue signal processing is dealt with on the Detector PCB.

The output from the detector is shown in the Diagnostics mode as a
Voltage, in mV. For more information see Section 5.2 - Detector
Circuit and Section 7.1 – The Diagnostics Mode.
This voltage display can be used to check lamp energy (ageing),

the correct functioning of the IR Stray Light filter as well as the
Dark Shutter.
320nm Energy, In the Diagnostics Mode (see Section 7.1

Diagnostics Mode) set the wavelength to 320nm, close the Dark
Shutter by pressing the right arrow key. Allow the mV reading to
stabilise and record the stable value.
Open the dark shutter by pressing the right arrow key and the mV
reading must increase by more than 4mV from that recorded above.
If not the lamp should be changed, if it still has not improved then
the condition and alignment of the optical components should be
checked.
Dark Current, Set wavelength to 320nm, Dark Shutter closed, IR

stray light filter closed, Voltage Display should be zero +/- 6mV.
If not and no light leaks are obvious (damaged seals around sample
chamber, lid not closing fully, damaged or poorly fitted casework
etc) then the detector or detector PCB may be faulty.
720nm Output, Set wavelength to 720nm, Dark Shutter open, IR

stray light filter open, Voltage Display must not be greater than
3600mV.
If greater than 3600mV check the lamp, lamp supply voltage, other
power supply levels and detector PCB.

2.5 Error Codes
See also Section 3.1 and 3.2 of the Instruction Manual
A number of Error Codes are generated that relate to various fault

conditions, these are detailed below with a brief description of
some of the most common causes for these errors.
Err 1, Dark level too high during a functional calibration. In

normal operation the dark shutter closes during an operator
instigated calibration sequence to ensure that the detector output is
below a threshold level. The calibration is aborted and Err 1
indicated if the detector output is above this threshold level. The
most likely cause is that the sample chamber door has been left
open or was opened during the calibration sequence, it may also
occur due to a faulty detector PCB.
Err 2, Light level too low during a functional calibration. This may
be caused by the sample/calibrant being too optically dense (dark),
it may also be caused by lamp failure as well as the use of plastic
or glass cuvettes at wavelengths where these materials will absorb
a high proportion of the light energy.
Err 3, Standard out of range in a concentration measurement. This

may be because the standard is too optically dense at the
wavelength selected or it may be too similar to the blank. Dillution
of the sample may be applicable for the former or the wavelength
for the later method may require adjustment.
Err 4, No zero order (white) light found during wavelength

calibration in start up routine. This error is given at the end of the
wavelength calibration routine, indicating that the test was partly
successful (see Section 6.1 Start Up Routine). Hence this error is
normally caused by low energy levels due to lamp ageing or
contamination/deterioration of other optical components. Also
ensure that a sample or cuvette has not been left in the sample
chamber during the start up tests and that there is no splashing or
spillage on the exit and detector lenses.
Err 5, No zero order (white) light found during wavelength

calibration in start up routine. This error is given during the
wavelength calibration routine, indicating that even the initial
threshold level was not achieved. Possible causes are; incorrect

lamp fitted, cuvette/sample or other obstruction in the light path
through the sample chamber as well as a possible optical
component failure or misalignment.
Err 6, No dark level found in start up routine. Leaving the sample

chamber open during the start up routine or opening it once the
procedure has started can cause this error. It can also indicate the
failure of the dark shutter solenoid, the solenoid drive or the
associated control circuits.
Err 7, Grating position sensor not detected. This error occurs

during the wavelength calibration procedure in the start up routine
and is generated at the beginning of this procedure. The grating is
driven in a clockwise direction until the attached vane breaks the
path of an opto-coupler. This is effectively the ‘end-stop’ and the
point from which auto-calibration will always start. Failure to
detect this point may be due to a faulty opto-coupler, wavelength
drive from the power supply PCB, a faulty motor or mechanical
coupling.
There are a number of special key functions for use by trained

engineers, do not use them unless you are fully conversant with all
the procedures these invoke.
Power On Reset. Hold the <Enter> key depressed while turning

on the power. This clears the operator set parameters held in non-
volatile memory and is useful in correcting many software
conflicts. As this procedure by passes the start up routine the
instrument must be re-booted before use.
Analogue Output Calibration. Hold the <up arrow> key

depressed while turning on the power. This enables fine calibration
of the analogue output against internally generated reference
voltages. See Section 8.6.
View Start Up Routine. Hold the <Cal> key depressed while

turning on the power. This enables the detector output and grating
position to be monitored on the display during the Start Up
Routine.

A to D Calibration. Hold the <Print> key depressed while turning
on the power. This enables the A to D converter on the detector
PCB to be calibrated against an external precision voltage source.
See Section 8.5.
Diagnostics Mode. Hold the <right arrow> key depressed while

turning on the power. The main display shows the signal from the
detector in mV and the resolution of the wavelength display is
increased to 0.5nm. See Section 7.1. Further special key functions
are invoked in the diagnostics mode as follows;
<right arrow> Further presses of this key will toggle the dark
shutter open and closed. See Section 7.2.
<left arrow > Alternate presses of this key will reduce the lamp
voltage to 5V (dim) and then return it to 12V. See Section 7.3.
<Cal> This key is used to set the wavelength display to 0.0 with
the grating in a position where white light (zero order) is reflected
on to the detector. A second press of this key is required to confirm
the setting before resuming the normal diagnostic functions. See
Section 7.4.
<print> Alternate presses of this key will toggle the Infra-red filter
in and out of the light path. See Section 7.2.
2.7 Test Solutions

1. Holmium Perchlorate – 5% w/v solution of Holmium Oxide in 1.4N
Perchloric acid, this will give absorbance maxima at 361.4, 416.1,
451.1, 485.3, 536.5 and 640.5nm.
2. Potassium Dichromate – 100.0mg/l in 0.005M Sulphuric Acid (use the

Sulphuric Acid as the blank). This will give an Absorbance value of
1.071 at 350nm.
Potassium Dichromate – 50.0mg/l in 0.005M Sulphuric Acid (use the
Sulphuric Acid as the blank). This will give an Absorbance value of
0.536 at 350nm.
3. Sodium Nitrate – 50g/l in deionised water, should give less than 0.1%
Transmittance at 340nm.
All these solutions are hazardous and the manufacturer/suppliers

safety precautions should be carefully followed at all times in
preparation, use and storage.

Section 3
System Description
3.1 Background
3.2 Sub-Assemblies
3.3 Accessories
3.4 Outputs

3.1 Background
The model 6300 is a single beam, visible spectrophotometer with
Absorbance, Transmission and Concentration measurement modes.
It is a direct replacement for the earlier model 6100.
This manual covers the service, maintenance and repair of all units
with a serial number greater than 5000. For the service,
maintenance and repair of units with serial numbers less than 5000
please refer to the 6300 Mark 1 Service Manual.
3.2 Sub-Assemblies
The model 6300 spectrophotometer can easily be broken down into
sub-assemblies for the purposes of repair or replacement. All the
PCBs are easily removed, see Section 8.2 – Dismantling. The
monochromator is also a replaceable sub-assembly. There is a
range of sampling accessories that can be easily fitted and removed
from the sample chamber.
See Section 2.2 for details of the main sub-assemblies and Section
11 for details of other spare parts. The following paragraph lists the
sampling accessories available.
3.3 Accessories
The following sampling accessories are available, where necessary
additional service information for these accessories is available on
request. The development of other sampling accessories is
continuous, please check current brochures or www.jenway.com for up-
to-date information.
632 001 External Sipper Pump (230V)

632 031 External Sipper Pump (115V)
634 001 4 Position Cell Changer
630 020 Test Tube Holder (13mm diameter)
630 021 Test Tube Holder (25mm diameter)
630 022 Test tube Holder (16mm diameter)
630 005 20 to100mm Single Cell Holder
648 001 Water Heated Single Cuvette Holder

Other accessories include:
543 001 External 40 Column Printer

542 009 Interface Cable Kit
630 028 Dust Cover
037 201 Water/refrigerant Circulator for use with 648 001
3.4 Outputs
The 6300 has both analogue and RS232 outputs.
Details of the level of the analogue output for the different ranges
that may be selected on the instruments is given in Section 6.3 of
the Instruction Manual.
Pin configuration for the RS232 socket is given in Section 6.2 of

the Instruction Manual. Section 6.1 of the Instruction Manual gives
details of the various ASCII codes that may be transmitted to the
6300 to enable complete remote control from a terminal or PC.

Section 4
Optical Description
4.1 Light Source
4.2 Grating
4.4 Signal Detector

4.1 Light Source
The 6300 uses a single Tungsten Halogen lamp to cover the full
wavelength range from 320 to 1000nm. This is a pre-focussed lamp
selected for the accurate position of the filament, making re-
alignment after replacement unnecessary.
The use of lamps other than those supplied by Jenway Ltd (part
number 012 075) will cause error codes to be displayed and result
in erroneous results. See Section 4.2 of the Instruction Manual.
4.2 Grating
From the lamp the light passes through a slit and lens assembly
then onto a flat holographic grating with 1200 lines per millimetre.
The grating is directly coupled to the stepper motor, which is under

microprocessor control. An opto-coupler mounted just behind the
grating acts as an ‘end stop’ when the sensor vane connected to the
grating mount blocks the light path across it.

The diffracted spectrum then passes through a further slit and lens
arrangement after which the light beam can be blocked by a shutter
switched in and out by an electronically operated solenoid.
Before passing into the sample chamber the monochromatic light

passes through an infra-red filter when wavelengths below 360nm
are selected. Similar to the shutter this filter is switched in or out by
an electronically operated solenoid under microprocessor control.
.
4.4 Signal Detector
An S1133 photo-diode is used as the detector on the 6300, it is
mounted directly onto the detector PCB, behind the lens block
The detector PCB carries out all of the analogue signal processing,
including the A to D conversion, and has its individual calibration
data stored in an on-board E2PROM. For more information see
Section 5.2 – Detector Circuit.

Section 5
Electronic Description
5.1 Power Supplies

5.1 Power Supplies
See Section 9.1 Power Supply Schematic
The ac mains supply is reduced by the torroidal transformer giving
two low voltage outputs from the secondary windings.
First the 20V ac output from the transformer is rectified by BR1;
the 30V dc output from this rectifier is used as the input to two
L4960 switch mode regulators. (REG1 and REG 3).
Tungsten Lamp Supply, The output of the switch mode regulator

REG 3 is set to 12V dc by VR1. The 12V output can be reduced to
5.1V by a logic ‘1’ on the gate of TR2. This happens during the
start up tests to reduce the light level for accurate zero order
detection, it can also be manually instigated by pressing the left
arrow key when in the Diagnostics Mode.
Solenoid and Fan Supply, The output of the switch mode

regulator REG1 is pre-set to 12V. The output to the fan is via Sk1
pin 1 and 2.
The output to solenoid 1 (Dark Shutter) is via SK9 pins 1 and 2

The output to solenoid 2 (IR Stray Light Filter) is via Sk9 pin 3 and
4.
5V Digital Supply, The other transformer secondary output is half

wave rectified and regulated to 5V by the linear regulator REG 2.
The output is distributed via SK5 pin 1.
Ancillary, Supplies to the end stop opto-coupler are connected via

SK2 pins 1 to 4.
The RS232 output, accessible through the rear panel on SK6, is fed
from the microprocessor PCB via SK5.
IC1 and 2, TEA3717DP, are the stepper motor drivers, controlled

from the microprocessor PCB via SK5 pins 9 to 12, with outputs to
the motor on SK2 pins 5 to 8.

Detectors and Amplifiers
The current through the detector D1 (S1133 type fitted) is

proportional to the incident light. IC3a acts as a current to voltage
converter, the gain being set by the feedback resistors in the T
network.
In normal operation pins 3 and 4 of SK2 are linked so the signal

passes to the three amplifiers of IC1. IC1b is set for unity gain,
IC1c has a gain of 10 and IC1d a gain of 100.
A to D Conversion
Each of these amplified signals then pass into the first three
channels (CH0 to CH2) of an 8 channel, 12 bit, serial, A to D
converter.
All three channels are converted and the microprocessor selects the
channel that gives the best resolution without reaching saturation
(32767 counts). In effect this means CH2 will be selected for inputs
up to 40mV, CH1 for inputs up to 400mV and CH0 for inputs up to
4.0V.
The A to d converter requires a reference voltage of 4.096V which

is generated from the 5V rail by D4, this is fed to pin 14, Ref+, of
the A to D converter, IC2.
The E2PROM, IC4, maintains calibration data for the PCB, see
Section 8.6 - A to D Calibration.

The microprocessor (IC102) is an H8-325 type with 64K linear
address space, its architecture is register based and optimised for
software written in the C language. A 16 bit address bus is used
with an 8 bit external data bus. The 1024 bytes of internal RAM are
shared equally between the internal requirements of the processor
and for the storage of operator set variables. The later are also
stored in non-volatile E2PROM to enable the last settings to be
restored after power has been interrupted or switched off.

The operating system software is stored in the 64K by 8 bit
EPROM, IC106. This will have a label attached identifying the
software version and date it was programmed. Removal of this
label may damage the component and will invalidate the warranty.
IC103 is a 3 to 8 line address decoder that can select the peripheral

devices on the data bus, being the keypad interface through IC104
or the display driver IC200.
The watchdog circuit (IC100) monitors the activity of the

processor, while IC101 generates the correct levels for the RS232
output. The four amplifiers in IC300 form the D to A converter
which generates the analogue output.

Section 6
Software and Operation
6.0 Warning
6.2 Photometrics
6.3 Concentration

6.0 Warning
This section gives an overview of the software functions, it is not
meant to be a detailed analysis of the software routines or code. It
must not be treated as a substitute Instruction Manual, its purpose
is to enable basic navigation through the operation and set up of the
6300 spectrophotometer sufficient to verify basic operation.

When the power to the unit is switched on a self-test routine is
activated. During these start-up tests the following parameters are
checked and must be passed before operation can continue:
Dark Level Test: - The light beam is blocked by the dark shutter
solenoid switching in. This test checks that the output of the
detector is below a threshold level when there is no light falling on
it. The test will be failed if the sample chamber lid is left open
during the start up routine, if the dark shutter (solenoid 1) is faulty
(electrical or mechanical) or if the Detector or Detector PCB is
faulty.
Wavelength Calibration: - This test checks for the zero order

(white) light that is reflected through the sample chamber when the
grating is in a position where it acts as a mirror. Each time the unit
is switched on this position is used as a physical reference point
against which the stored wavelength calibration data is applied.
This test is carried out in the following manner; the microprocessor

instructs the grating to drive to its minimum value of –200nm. This
should ensure that the vane attached to the grating mount breaks
the light path of the opto-coupler mounted on the monochromator
base plate that is set at a position of approximately –50nm.
If it does not receive a signal from the opto-coupler then error code
Err 7, grating position sensor not detected, is returned when the
motor stops.
In correct operation a signal is returned when the vane reaches the

opto-coupler. Then the microprocessor instructs the grating to
rotate in the reverse direction, in 1nm, steps for 200nm, or until a
signal greater than 200mV is returned from the detector (this level,

with the lamp dimmed can only be produced by the white zero
order light). If this 200mV level can not be achieved then the
grating stops after having rotated the 200nm and an error code Err
5, no peak light level found, is returned.
In correct operation when this 200mV level is detected the grating

rotates in the same direction for a further 200nm but in 0.5nm
steps. For each step an increasing output is returned until the peak
is reached. The grating is stopped when a decrease in output is
measured and by reversing again for one step the position of the
peak is verified. If the grating rotates the full 200nm without the
output increasing and then falling the error code Err 4, no zero
order peak found, is returned.
This test will be failed if samples or cuvettes etc are left in the
sample compartment during the start up routine, if the sample
holder or sampling device in the sample chamber is incorrectly
fitted/aligned such that it obscures the light beam. Also if the
incorrect lamp is fitted, if the lamp has not been fitted correctly, if
the dark shutter is (stuck) in the closed position or through
contamination, degradation or misalignment of other optical
components.
6.2 Photometrics
When the Start Up test has been successfully completed the display
returns to the last settings of wavelength and mode used before the
instrument was turned off.
The wavelength displayed on the lower digital display can be

adjusted by using the up and down arrow keys to select the value
suitable for a specific application.
The cursor can be moved along the menu bar at the bottom of the
screen by using the right and left arrow keys.
Moving the cursor under the %T icon will enable the Transmission
measurement mode, similarly moving it under the ABS icon will
enable the Absorbance measurement mode. These two modes are
calibrated by simply inserting a cuvette or test tube containing a
blank solution in the sample chamber, closing the lid and pressing
the CAL pushbutton. The CAL icon on the display will flash while

the calibration sequence is in progress; when it stops the main
digital display will be set to 100.0 %T or 0.000 ABS automatically.
Sample measurement can then be carried out by inserting a cuvette

or test tube containing the sample solution into the sample
chamber, closing the lid and recording the value on the main
display.
6.3 Concentration
Moving the cursor under the CONC icon on the bottom menu bar
enables the concentration measurement mode.
NOTE:- Before selecting this mode a blank calibration must be

carried out in the Absorbance mode at the wavelength to be
used for the concentration measurement.
When the concentration mode is selected two further icons, xF and

UNITS, appear on the bottom menu bar.
A linear relationship between Absorbance and Concentration is

assumed for all measurements. This enables concentration
measurements to be made against a standard solution (CONC
mode) or by entering a factor which adjust the slope of the straight-
line relationship (xF mode). The latter is also often used to improve
the sensitivity of comparative colour measurements in a number of
International Standard Methods, as in beer colour measurements to
EBC standards etc.
A range of units can be displayed against the top digital

(Concentration) display. The required units can be selected by
moving the cursor, with the right or left arrow keys, along the
bottom menu bar until it is under the UNITS icon. The Up or Down
arrow keys can then be used to display in turn ppm, mg/l, g/l, M, %
or blank (no units displayed). When the required unit is displayed
simply move the cursor back to xF or CONC)
To calibrate the concentration range against a known standard

move the cursor beneath the CONC icon using the right or left
arrow keys. Insert a cuvette or test tube containing the known
standard solution into the sample chamber, close the lid and press
the CAL key. The CAL icon on the display will flash and the up or

down arrow keys can be used to adjust the value displayed on the
upper digital display to the actual value of the standard being used.
When this value has been set a further press of the CAL key
completes the calibration against this standard solution. Cuvettes or
test tubes containing samples can then be inserted in the sample
chamber, the sample chamber lid closed and readings taken directly
from the display.
During the above calibration procedure the software calculates the

factor required to multiply the Absorbance by to achieve the linear
relationship with the concentration of the standard used. The Factor
(xF) mode is automatically updated with this value which can be
viewed by moving the cursor, using the right or left arrow keys,
along the bottom menu bar until it is under the xF icon. For future
assays this factor could be used instead of the standard solution but
good practice and standard operating procedure (sop) should be
observed and the accuracy of this factor be verified on a regular
basis.
If a factor is known or supplied in a sop then this can be entered

directly in the Factor (xF) mode by moving the cursor, using the
right or left arrow keys, along the bottom menu bar until it is under
the xF icon. The up or down arrow keys can then be used to adjust
the value of the factor on the lower digital display from 0 to 9999,
when the correct value has been set move the cursor back under the
CONC icon using the left arrow key. Cuvettes or test tubes
containing samples can then be inserted in the sample chamber, the
lid closed and readings taken directly from the display.

Section 7
Diagnostics
7.1 The Diagnostics Mode
7.3 Lamp Control
7.4 Zero Order Calibration

7.1 The Diagnostics Mode
The Diagnostics mode is accessed using the following Special Key
function, hold down the right arrow key <>> while turning the
power on. The main display now shows the linearised signal from
the detector in mV and the resolution of the wavelength display has
increased to 0.5nm.

Once the diagnostics mode has been activated further presses of the
right arrow key <>> will toggle the dark shutter alternately
between the open and closed positions. With the covers still on the
instrument the actual position of the dark shutter can be determined
by the main display that will in general have a very low (zero)
reading when the shutter is closed and a very high reading when
the shutter is open.
Once the diagnostics mode has been activated alternate presses of

the print key will toggle the Infra-red filter in and out of the light
path. A similar effect to the above can be used to determine the
actual position of the filter when the instrument covers are in place;
of course the difference between the ‘in’ and ‘out’ readings will be
greater at higher wavelengths.
7.3 Lamp Control

Once the diagnostics mode has been activated alternate presses of
the left arrow key (<<>) will reduce the lamp voltage to 5V (dim)
and then return it to 12V. This function is automatically used in the
start up tests to accurately detect the zero order position. For safety
reasons it should also be used at all times when working on the
instrument with any covers removed such that the lamp is exposed.
7.4 Zero Order Calibration

Warning;-
This procedure can be used to re-set the stored wavelength
calibration data, so a new wavelength calibration must be carried

out after this procedure has been used. Also see Section 8.3 Optical
Alignment
1 Press the right arrow key [>] while turning the power switch on.
2 The display should show a mV reading on the top row, followed by

the wavelength on the 2nd row and %T at the bottom left hand side.
3 Pressing the right arrow key again [>] should operate the dark
shutter solenoid. Press this key to ensure that the shutter is in the
open position (the position that gives a maximum reading on the
display)
4 Press the down arrow key until the wavelength display reads 0.0.
Press the left arrow key to dim the lamp.
5 Use the up and down arrow keys to move in 0.5nm steps either side
of zero and identify at which wavelength a peak mV reading is
obtained. Note this reading.
6 Pressing the CAL key will re-set this reading to zero and the CAL
symbol on the display will flash. While it is flashing carry out step
7 or step 8.
7 Pressing the CAL key again will clear the wavelength display
offset noted in step 5 to zero.
8 The offset noted in step 5 (or an alternative correction, see Section

8.5 Wavelength Calibration) can be entered before pressing the
CAL key by using the up or down arrows to set the display to the
required offset.
9 If step 7 was used a wavelength calibration must be carried out; in

both cases a performance verification as detailed in Section 8.8
must be carried out.

Section 8
Maintenance
8.2 Dismantling
8.4 Energy Levels

The Jenway Limited, Model 6300 Spectrophotometer has been
designed to give optimum performance with minimal maintenance.
It is only essential to keep the external surfaces clean and free from
dust and to ensure that the area around and underneath the unit is
also clean and dust free.
The sample area should be kept clean and accidental spillage

should be wiped away immediately as some corrosive or solvent
based samples or standards may attack the materials used in the
sample chamber and cell holders.
To give added protection when not in use the unit should be

disconnected from the mains supply and covered with the optional
dust cover (630 028). For longer term storage it is recommended
that the unit be returned to the original carton, for re-shipment a
further external packing case suitable for the method of carriage
should be used.
Details of all routine maintenance tasks, including changing the

lamp can be found in Section 4 of the Instruction Manual.
8.2 Dismantling
Before dismantling any of the following sub-assemblies ensure that
the unit is switched off and the power cable is disconnected from
the supply
Do not attempt to dismantle these units unless they are in a clean,

dry and dust free environment.
Use a soft lint free cover on any benches that will have casework,
displays or keypads placed on them.
Use approved and tested anti-static procedures when dismantling

any electronic sub-assembly or PCB and store these items in anti-
static containers where necessary.
General – Access to all major sub-assemblies can easily be gained

by removing the top half of the case. Access to the lamp housing
can be made through the lamp access panel on the rear of the unit.

The sampling accessory can be accessed through the sample
chamber lid.
Top/Bottom Case Assemblies – The top and bottom case

assemblies can easily be separated by unscrewing the four recessed
screws in each corner of the base. This should be done without
inverting the unit, by moving it forward over the front edge of the
bench to unscrew the front two screws, and then turning it around
to do the same with the back two. Turn it back round and then the
top half of the case can be lifted off the bottom half take care not to
strain any cables between the top and bottom sections.
Should it be necessary to work on the top case assembly by itself it

is simply a matter of disconnecting the plug from SK5 on the
power supply PCB then the top can be completely removed.
Microprocessor/Display PCB The microprocessor/display PCB is

mounted in the top case assembly. To remove it disconnect SK1 on
the ribbon cable to SK5 and PL106 to the membrane keypad.
Unscrew the four screws and the PCB can be removed. The display
module and microprocessor PCB should be treated as a pair and
replaced together. Removing the display from the PCB should not
be attempted.
Detector PCB The detector PCB is mounted vertically at the far

right hand side of the lower chassis. It is easily removed by
unscrewing the two screws recessed in the top of the metal
mounting block. SK4 on the power supply PCB should be removed
and the ribbon cable pulled back under the monochromator (it may
be necessary to lift the monochromator to complete this task).
Remove the two screws and spacers that hold the lens block, take
care not to rotate it as the detector is mounted in a recess inside the
block and can easily be broken off. Remove the last screw with its
nut and washer to enable the electrostatic screen to be removed
from the PCB.
The Detector PCB stores detailed calibration data relating to the

optics of the unit it is fitted in, replacing the detector PCB without
a full re-calibration will invalidate the quoted specification.
Monochromator The monochromator is located across the front of

the lower case. It is a sealed unit and breaking the seals will

invalidate the warranty. Before proceeding with replacement
ensure the unit is in a clean, dust and humidity free area.
Remove the four screws from the base plate, two at the front, one
at the far right hand side in front of the detector PCB the other on
the far left hand side. Carefully lift the monochromator assembly
unplugging the connectors SK1 and SK9 without straining the
cables. The monochromator can now be removed.
Replacement is the reverse of dismantling, but ensure that all

cables are carefully fitted in the appropriate recesses so that they
are not crushed when screwing the unit down. When fitting a
replacement unit ensure a full calibration is run so that the new
calibration data for the new monochromator is stored. (See Section
8.5, 8.4 and 8.7)
Power Supply PCB With the monochromator removed as above

the power supply PCB is easily removed by undoing the four
screws holding it to the lower case assembly. SK3 to the
transformer and SK1 to the fan should be removed before lifting it
out of the lower case.
Cooling Fan Carry out the above procedures to enable the fan to
be lifted out of its recess in the lower case. Ensure the position of
the retaining spring and the direction of the airflow are noted for
re-assembly.
Transformer Remove SK3 from the power supply PCB and the
push on connectors for the cables that go to the mains switch and
inlet filter assembly. Then remove the transformer by undoing the
single bolt through the centre that holds it to the moulded bracket
in the base assembly.

This procedure can irreversibly affect the performance and
operation of the instrument. Please read the following warnings
and request help or clarification before proceeding.
Carrying out this procedure will invalidate the manufacturers

optical performance specification and should only be

undertaken by personnel trained and equipped to verify the
optical performance of the instrument.
Instrument covers should only be removed by engineers

trained in safe working practices and aware of electric shock
hazards. Removal of the monochromater cover will invalidate
any warranty claim regarding performance to specification.
Do not look directly at the light source, use eye protection or

the lamp dimming function when necessary. Do not touch any
optical surfaces.
If in any doubt DO NOT PROCEED.
Ensure or verify by replacement that a GENUINE JENWAY

LAMP is fitted, (refer to the instruction manual for details).
There are many lamps that look similar but the filament
position is critical and can only be guaranteed on Jenway
lamps, replacing the lamp may correct the problem without
any further re-alignment.
1 Remove the top half of the case by undoing the four recessed
screws in the corners of the base.
2 Place the top half of the case behind the base taking care not to trap
or strain the connecting cables.
3 Remove the two screws retaining the black monochromator cover

in the bottom half of the case. Remove the cover and place to one
side. DO NOT TO TOUCH ANY OPTICAL COMPONENTS.
4 Taking all precautions to avoid the risk of electric shock, connect

the power cable and depress the right arrow key [>] while turning
the power switch on.
5 The display should show a mV reading on the top row, followed by

the wavelength on the 2nd row and %T at the bottom left hand side.
6 Pressing the right arrow key again [>] should operate the solenoid
in front of the exit slit assembly. Press this key to ensure that the
shutter is in the open position (light is passing through the exit slit)

7 Pressing the left arrow key will dim the light source. This should
always be done when looking at or towards the light source. For
now ensure it is pressed to give maximum light output.
8 Follow the light path from the lamp through the entrance slit
assembly on to the grating. Use a narrow strip of white card to help
show the position and shape of the light beam. DO NOT TOUCH
ANY OPTICAL COMPONNENTS.
9 Move the card in an arc in front of the grating from the exit slit
assembly, through the incident light beam and towards the torroidal
transformer at the back of the instrument.
10 While doing this identify the bright coloured spectrum (1st order),
the white incident light beam, the white zero order light beam, and
then the dimmer coloured spectrum (2nd order).
11 Press the up or down arrow key to move the white zero order light
beam towards the exit slit. (Usually the down arrow key). The
wavelength displayed should be seen to decrease towards zero.
12 Keep pressing the down (or up) arrow key until the white zero
order light is positioned exactly and symmetrically across the exit
slit.
13 Press the print key to switch the Infra-red filter out of the light path
and check, using the white card that this light passes the sample
chamber and onto the detector lens.
14 With the zero order light passing through the sample area onto the
detector press the <CAL> key twice and ensure that the
wavelength display reads zero.
15 Replace the black monochromator cover, tighten the two retaining

screws and place the top half of the case on the base taking care not
to trap any cables.
16 Press the up and down arrow keys and check to see if the mV
reading can be increased as the wavelength changes. If the display
goes over-range (1. ) Press the left arrow key to reduce the lamp
brightness.

17 If a peak mV reading can be obtained within +/- 2nm of the zero
point calibrated by this procedure then press the <CAL> key twice
when this peak mV reading is obtained. If the peak reading is
obtained at a wavelength greater than +2nm or less than –2nm then
the above alignment procedure should be repeated. When OK
replace the four screws in the base section.
18 A wavelength calibration must now be carried out (see Section 8.5)

and then a full performance verification as detailed in Section 8.8.
Grating Position Sensor
Operation and adjustment can be checked and carried out as follows;
Remove the top half of the case by undoing the four recessed screws in
the corners of the base.
Place the top half of the case behind the base, taking care not to trap or
strain the connecting cables.
Taking all precautions to avoid the risk of electric shock, connect the
power cable and depress the right arrow key [>] while turning the power
switch on.
The display should show a mV reading on the top row, followed by the
wavelength on the 2nd row and %T at the bottom left hand side.
Connect the positive lead of a voltmeter to the anode of D4 on the power

supply PCB and the negative lead to the black analogue output socket on
the rear panel. (The anode of D4 is the contact close to SK3 that is also
connected to the plate through hole between D4 and SK3). Select a range
suitable for measuring up to 5V dc.
Press the down arrow key to reduce the wavelength to –30nm, the
voltmeter should read approximately 0.100V, Press the down arrow key
again to reduce the wavelength to –40nm, the voltmeter should still read
approximately 0.100V.
Press the down arrow key again to reduce the wavelength to –50nm, the
voltmeter should now read approximately 5.00V. Press the up arrow key
and return the wavelength setting to –40nm and the voltmeter reading
should return to approximately 0.100V.

Should these results not be obtained the function of the opto-coupler
should be checked, this can be carried out as follows;
Remove the monochromator cover observing all the previously stated

precautions. Press the up arrow key to select a wavelength about 500nm
and check that the grating rotates correctly.
Pass a piece of thick paper or card between the ‘jaws’ of the opto-coupler
and check that when the card is inserted a voltage of approximately 5.00V
is measured as above and that when it is removed this voltage drops to
approximately 0.100V.
If these results are correct then the position should be adjusted as below,
if not then the opto-coupler or power supply PCB may be faulty. There
may also be a bad connection between them. Check the wiring to SK2
and that no wires are trapped under the monochromator.
To adjust the position press the down arrow key to set the wavelength to
–50nm, slacken the two fixing screws slightly and adjust the position of
the opto-coupler until the voltmeter reading just changes from 0.100V to
5.00V.
Tighten the screws and check that at –40nm the reading is approximately
0.100V and that at –50nm it is approximately 5.00V. If not repeat as
above.
8.4 Energy Levels

Equipment Required; - None, checked against internal settings.
Before proceeding with any calibration it is essential to ensure the correct

functioning of the optical system, this can be done very easily in the
Diagnostics mode (see Section 7) where the following performance
should be obtained.
All analogue signal processing is dealt with on the Detector PCB. The
output from the detector is shown on the Diagnostics Screen as a Voltage,
in mV. For more information see Section 5.3 - Detector Circuit and
Section 7.1 – The Diagnostics Mode.
This voltage display can be used to check lamp energy (ageing), the
correct functioning of the IR Stray Light filter as well as the Dark Shutter.

320nm Energy, In the Diagnostics Mode (see Section 7.1 Diagnostics
Mode) set the wavelength to 320nm, close the Dark Shutter by pressing
the right arrow key. Allow the mV reading to stabilise and record the
stable value.
Open the dark shutter by pressing the right arrow key and the mV reading
must increase by more than 4mV from that recorded above.
If not the lamp should be changed, if it still has not improved then the
condition and alignment of the optical components should be checked.
Dark Current, Set wavelength to 320nm, Dark Shutter closed, IR stray

light filter closed, Voltage Display should be zero +/- 6mV.
If not and no light leaks are obvious (damaged seals around sample
chamber, lid not closing fully, damaged or poorly fitted casework etc)
then the detector or detector PCB may be faulty.
720nm Output, Set wavelength to 720nm, Dark Shutter open, IR stray

light filter open, Voltage Display must not be greater than 3600mV.
If greater than 3600mV check the lamp, lamp supply voltage, other power
supply levels and detector PCB.

Equipment Required; - A certified wavelength standard, i.e. Holmium
Oxide Filter, Holmium Perchlorate Solution etc. (See Section 8.8.1)
Wavelength calibration can be carried out in the Diagnostics Mode using

the wavelength (zero order) offset function. Do not carry out the
following procedure without a suitable, certified wavelength calibration
standard.
Turn the unit on and allow the Start Up tests to complete.
Use the up and down arrow keys to select a wavelength about 10nm
below the certified wavelength of the filter or standard to be used.
Select the absorbance or transmission modes, using the right or left arrow
keys, depending on whether an Absorbance or transmittance standard or
filter is being used.

Press the CAL key to set the display to 0.000ABS or 100%T. Insert the
filter or standard in the sample chamber and close the lid.
Press the up arrow key to increase the wavelength by 1nm and check that
an Absorbance value has increased or a Transmission value has
decreased.
Repeat the above until the first Absorbance value decreases or the first
Transmission value increases. At this point press the down arrow key and
check that the previous value is attained again. Then record this
wavelength as the reported peak.
Repeat the above and check that the same value is reported.
NOTE: 1.) If it is known that there are other peaks closer than 10nm
to the certified peak then the start wavelength should be moved closer to
the certified peak just past any others.
2.) If the Absorbance display goes over range during the test
then carry out the Calibration at the initial wavelength with the filter or
standard in the sample chamber.
Calculate the adjustment required to correctly align the reported figure

with the certified value from the following...
Certified Value – Reported Value = Correction factor (can be negative or

positive, maximum correction permissible is 3.0nm)
Switch the unit off and re-start it in the Diagnostics Mode by holding
down the right hand arrow key <>> while turning power on.
The display should show a mV reading on the top row, followed by the
wavelength on the 2nd row and %T at the bottom left hand side.
Pressing the right arrow key again [>] should operate the dark shutter
solenoid. Press this key to ensure that the shutter is in the open position
(the position that gives a maximum reading on the display)
Press the down arrow key until the wavelength display reads 0.0. Press
the left arrow key to dim the lamp.
Use the up and down arrow keys to set the display to the correction factor
calculated above (observe polarity)

Pressing the CAL key again will enter this value into memory as the new
wavelength calibration offset.
Switch the unit off and then on again, allowing the Start Up tests to be
completed, re-check the certified wavelength calibration standard as
above and check that the reported value is now correct.
The A to D converter should only be calibrated by engineers who have

been trained on this aspect of servicing by Jenway Limited.
Equipment Required; - A certified voltage calibrator with a resolution

of 0.1mV and a range up to at least +/-4.0000V.
A lead for connecting the calibrator to pins 1 (negative) and pin 3
(positive) of SK2 on the detector PCB. (8 pin Molex type connector)
Access the Detector PCB by removing the top case assembly as described
in Section 8.2 - Dismantling.
Remove the jumper from pins 3 and 4 on SK2 on the Detector PCB and
fit the lead connected to the calibrator.
Switch the calibrator on and select a negative output (or reverse the
contacts)
Select the A to D calibration mode by turning the unit on with the Print
key depressed.
The lower display will change to prompt for specific input levels and the
upper display will show the relevant mV output.
The initial prompt is for –1mV, set the calibrator to give an input signal
of –1mV, let the upper display settle and then press the enter key.
Then the prompt changes to –20mV, set the calibrator to –20mV let the
upper display settle and then press the enter key.
Continue responding to the prompts in this way for –39mV, -

200mV, -390mV, -2000mV and –3900mV. Note that the –39mV and –

390mV levels are repeated as these are the cross over points from one
channel to the next.
When successfully completed the display returns to the last settings used
in the measurement mode.
Equipment required; - Voltmeter capable of reading 2.0V with a

resolution of 1mV.
The D to A calibration sets the levels of the analogue output. This is

carried out with the on-board voltage reference at zero and +/- 2000mV.
Select the D to A calibration mode by holding the up arrow depressed

while the power is turned on.
The upper display will show ‘dAC mV’ and the lower display will
indicate the mV level that should be available on the Analogue output.
Connect a voltmeter to the analogue output on the rear panel. Select a

range that will display 2000mv to 0.1mV resolution.
The first prompt indicates an output level of -2000mV, use the up and
down and left and right arrow keys to adjust the actual reading on the
voltmeter to –2000mV.
The left and right arrow keys change the output in 5mV steps, the up and
down arrow keys in 0.5mV steps.
When the correct level is reached press the enter key and the prompt
moves on to 0mV, repeat the above for this and the 2000mV levels.
When successfully completed the display returns to the last settings used
in the measurement mode.

Equipment Required; - 1. Certified Wavelength Standard,
2. Certified Absorbance Standards, 3. Certified Stray Light
Standard.
Items 1 and 2 above can be supplied as Calibration Filter Sets, order part
numbers 035 088.
Where filters are not available the following reagents may be used:
8.8.1 Holmium Perchlorate – 5% w/v solution of Holmium Oxide in

1.4N Perchloric acid, this will give absorbance maxima at 361.4,
416.1, 451.1, 485.3, 536.5 and 640.5.
8.8.2 Potassium Dichromate – 100.0mg/l in 0.005M Sulphuric Acid (use

the Sulphuric Acid as the blank). This will give Absorbance values
of 1.071 at 350nm, 0.484 at 313nm.
Potassium Dichromate – 50.0mg/l in 0.005M Sulphuric Acid (use
the Sulphuric Acid as the blank). This will give Absorbance values
of 0.536 at 350nm, 0.242 at 313nm.
8.8.3 Sodium Nitrate – 50g/l in deionised water, should give less than
0.1% transmittance at 340nm.
All these solutions are hazardous and the manufacturer/suppliers

safety precautions should be carefully followed at all times in
preparation, use and storage.
8.8.4 Wavelength Verification

Equipment Required; - A certified wavelength standard, i.e. Holmium
Oxide Filter, Holmium Perchlorate Solution etc. (See Section 8.8.1)
Turn the unit on and allow the Start Up tests to complete then allow 15
minutes for the instrument to warm up.
Use the up and down arrow keys to select a wavelength about 10nm
below the certified wavelength of the filter or standard to be used.

Select the absorbance or transmission modes, using the right or left arrow
keys, depending on whether an Absorbance or transmittance standard or
filter is being used.
Press the CAL key to set the display to 0.000ABS or 100%T.
Insert the filter or standard in the sample chamber and close the lid.
Press the up arrow key to increase the wavelength by 1nm and check that
an Absorbance value has increased or a Transmission value has
decreased.
Press the up arrow key again to increase the wavelength by 1nm and
check that an Absorbance value has continued to increase or a
Transmission value has decreased further.
Repeat the above until the first Absorbance value decreases or the first
Transmission value increases. At this point press the down arrow key and
check that the previous value is attained again. Then record this
wavelength as the reported peak.
Repeat the above and check that the same value is reported.
NOTE: 1.) If it is known that there are other peaks closer than 10nm
to the certified peak then the start wavelength should be moved closer to
the certified peak just past any others.
2.) If the Absorbance display goes over range during the test
then carry out the Calibration at the initial wavelength with the filter or
standard in the sample chamber.
Check that the reported peak wavelength falls within the specified
tolerance of the instrument PLUS the tolerance of the filter or standard
used.

8.8.5 Absorbance Verification
Equipment Required; - Certified Standard Absorbance Filters or

Potassium Dichromate solution. (See Section 8.8.2)
Use the up or down arrow keys to select a wavelength at which the filter
or solution is certified.
For the Potassium Dichromate solution use the Sulphuric Acid solution as
a blank, (See Section 8.8.2) if the filter set includes a zero filter use this
as the blank, if not set the blank (zero absorbance or 100% transmittance)
with an empty sample chamber.
Insert the blank (ref. above paragraph) into the sample chamber and close
the lid. Press the CAL key and ensure the display reads 0.000 ABS or
100% T. Remove the blank from the sample chamber.
Insert the certified filter or Potassium Dichromate solution and check that
the reading is within the specified tolerance of the instrument PLUS the
tolerance of the filter/solution used.
Repeat this for other filters or solutions and at other specified

wavelengths as necessary.

8.8.6 Stray Light Verification
Equipment Required; - Certified Stray Light Filters or Sodium Nitrate
Solution or Sodium Iodide Solution. (See Section 8.8.3)
Select a wavelength at which the filter or solution is certified (340nm for

Sodium Nitrate)
Select the Transmission mode by using the right and left arrow keys to
move the cursor on the bottom menu bar under the %T icon.
Fill a cuvette with the deionised water used to make up the solutions and
place it in the sample chamber. Or for a filter standard use the blank filter
supplied by the manufacturer
Press the CAL key and ensure the reading is 100.0%
Insert the stray light filter or solutions, as above, and ensure that the
reading is within the specified tolerance of the instrument PLUS the
tolerance of the filter/solution used.

Section 9
Circuit Diagrams
9.1 Power Supply Schematic 630 504
9.2 Power Supply Layout 630 504
9.3 Detector PCB Schematic 630 506

9.4 Detector PCB Layout 630 506
9.5 Microprocessor PCB Schematic 630 013

9.6 Microprocessor PCB Layout 630 013

Section 10
Assembly Diagrams
10.1 6300 Final Assembly 630 503
10.2 6300 Lower Case Assembly 630 510
10.3 6300 Top Case Assembly 630 010
10.4 6300 Optics Assembly 630 508
10.5 6300 Rear Panel Assembly 6630 012

Section 11
Spare Parts
11.01 Packed Instrument
11.02 Top Case Assembly
11.03 Microprocessor PCB
11.04 Lower Case Assembly
11.05 Monochromator Assembly
11.06 Detector PCB
11.07 Power Supply PCB
11.08 Rear Panel Assembly

6300 SPARE PARTS LIST
Part Number Drwg/Cct Description

Ref
Section 11.01 630 501 Packed Instrument.

033 227 Packing case complete with inserts
060 084 Disposable cuvettes (4ml) pack of 100
630 026 Instruction manual
013 046 Mains cable without plug
013 083 Mains Cable U.S.A. plug
013 123 Mains Cable European plug
013 181 Mains Cable U.K. plug
TBA Service Manual
630 028 Dust Cover
Section 11.02 630 010 Top Case Assembly.

630 025 Membrane keypad
630 032 Sample Chamber Lid
Section 11.03 630 013 Microprocessor PCB.

630 013 Micro-processor PCB
630 512 EPROM (Programmed)
Section 11.04 630 510 Lower Case Assembly.

010 040 Torroidal transformer assembly
060 040 Large rubber feet
060 311 Cooling fan 12V 60mm
Section 11.05 630 508 Monochromator Assembly.

009 063 Tungsten halogen lamp base
012 075 Tungsten halogen lamp
032 005 Solenoid 12V dc
630 516 IR Filter 6300
012 089 Chassis mount optocoupler
Section 11.07 630 506 Detector PCB.

630 506 Detector PCB assembly 6300
002 054 470uF 16V radial electrolytic capacitor
012 085 Photodetector 6300
020 002 Voltage regulator 79L05
020 006 Voltage regulator 7805
Section 11.08 630 504 Power Supply PCB.

630 504 Main power supply PCB

005 024 Bridge rectifier
020 027 L4960 voltage regulator
006 115 25 way D socket
009 124 4mm socket red
009 125 4mm socket black
Section 11.10 640 006 Rear Panel Assembly.

009 123 Mains input socket
016 021 2A fuse 20mm (for 220V supply)
062 241 Lamp Panel Retaining Screw
017 050 Switch rocker 2p

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Jenway 6300

Spectrophotometer Service Manual

Section 2 Quick Reference

Section 3 System Description

Section 4 Optical Description

Section 5 Electronic Description

Section 6 Software and Operation

Section 9 Circuit Diagrams

Section 10 Assembly Diagrams

Section 11 Spare Parts List

Jenway 6300 Ser Man 1

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1.0 Index to Sections

1.1 About This Manual

1.2 Using This Manual

1.3 Warnings & Safe Practice

1.4 Standards & Certification

1.5 Ordering Spares

1.6 Returning Items

1.7 Contacting Jenway Limited

Jenway 6300 Ser Man 2

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1.0 Index to Sections

Section 2 - Quick Reference

Section 3 - System Description

Section 4 - Optical Description

Jenway 6300 Ser Man 3

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Section 6 - Software and Operation

Jenway 6300 Ser Man 4

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Section 10 - Assembly Diagrams

Section 11 – Spare Parts List

Jenway 6300 Ser Man 5

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1.2 Using This Manual

Updates to this manual will be circulated through the Jenway Ltd

In practice Section 2 - Quick Reference and Section 8 –

To find the information required refer to the Main Index or Index

1.3 Warnings & Safe Practice

Jenway 6300 Ser Man 6

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Finger marks, dust and condensation can quickly destroy sensitive

Many of the reagents, solutions and standards used for maintenance

This instrument can be used for analysing a broad range of

1.4 Standards & Certification

1.5 Ordering Spares

Jenway 6300 Ser Man 7

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1.7 Contacting Jenway Limited

Emails should be sent to sales@jenway.com

Fax: +44 1371 821083

Phone: +44 1371 820122

Please note no items can be returned to (or will be accepted by)

Jenway 6300 Ser Man 8

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2.0 About ‘Quick Reference’

2.2 Main Sub-Assemblies

2.3 Power Supply Voltages