Sample Prep Catalog1

THEEssential
CHROMATOGRAPHY & SPECTROSCOPY

CATALOG
This information is subject to change without notice.
11
© Agilent Technologies, Inc. 2011
12
EDITION
SAMPLE PREPARATION
PAGES 140-249
SAMPLE PREPARATION
140 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

In this Chapter
Solid Phase Extraction (SPE)

152 Bond Elut Plexa
161 Polymeric SPE
166 Silica-Based SPE
190 Inorganic SPE
193 Specialty SPE
202 Diatomaceous Earth Sorbents
204 Bulk SPE
206 Bond Elut Accessories
220 Disk SPE
225 Micro-volume SPE
228 QuEChERS
235 Captiva Filtration
242 Sample Filtration
248 TOXI-TUBES
SOLID PHASE EXTRACTION (SPE) 141

Sample Preparation
Application Guide
Industry Application Technique Product
Pharmaceutical Bioanalysis Solid Phase Extraction Bond Elut Plexa
Bond Elut Plexa PCX
Bond Elut Plexa PAX
Bond Elut
Mega Bond Elut
OMIX
SPEC
Liquid/Liquid Extraction Chem Elut
Protein Precipitation Captiva NDLipids
Filtration Captiva
Supported Liquid Chem Elut
Extraction (SLE)
Biotechnology Protein/Peptide Lysate Filtration Captiva
Purification Micro-volume SPE OMIX
Clinical and Forensic Bioanalysis Solid Phase Extraction Bond Elut
Bond Elut Plexa
Bond Elut Plexa PCX
SPEC
OMIX
Extraction (SLE)
Protein Precipitation Captiva NDLipids
Filtration Captiva
Environmental Monitoring Semi-volatiles Solid Phase Extraction Bond Elut
SPEC
Oils and Grease Solid Phase Extraction Bond Elut
SPEC
Water Removal Bond Elut
Na2SO4
Food and Beverage Pesticides Solid Phase Extraction Captiva
and Herbicides Bondesil Bulk Silica
QuEChERS
Extraction (SLE)

Sample Preparation
SOLID PHASE EXTRACTION (SPE)
Agilent Bond Elut:

Accuracy Starts Here
For over 30 years, Bond Elut has been the most trusted name in solid phase extraction. Years of
use by demanding chemists at top companies worldwide have thoroughly documented its many
applications and proven its performance. To this day, you will find more literature references for
Bond Elut than any other SPE product in the industry.
Bond Elut is manufactured using state-of-the-art automation to guarantee quality and
consistency. Optical scanners installed throughout our automated assembly process inspect
each Bond Elut tube at multiple points. And during manufacture, 25 different tests are conducted
to ensure reproducibility. If an imperfection is spotted, the tube is removed from the assembly
line. The result is consistently reliable Bond Elut cartridges, time and time again.
Over 40 different sorbent functionalities are available in a variety of cartridge formats including
straight barrel, large reservoir capacity (LRC) and Bond Elut Junior (Jr).
The Bond Elut Difference

• Heritage of Reliability: With years of use in some of the most demanding analytical
laboratories in the world, Bond Elut products have a proven track record resulting in a strong
publication pedigree
• Options for Your Needs: Offering extraction solutions for the widest range of analytes
and matrices, with over 40 bonded silica phases for high specificity methods and polymeric
phases for rapid method development, Bond Elut has the largest choice of formats and
sorbents in the market
• Innovative Products Designed for Lab Efficiency: Whether it be fast flow polymeric
particles or our patented 96-well plate design, all Bond Elut products are created for
ease-of-use and flexibility to meet both manual and automated requirements
• Technical Support at Every Step: For your specific applications, or to help solve
occasional technical issues, a global team of analytical scientists is on hand to assist
• World Class Manufacturing and Quality: Unrivaled manufacturing control, plus exacting
ISO 9001: 2000 compliant inspections guarantee the consistent quality of Bond Elut

Sample Preparation
Sample Preparation Formats
Agilent Offers the Broadest Range

of Tube Formats and 96-well Plate Designs
We have a full set of straight barrel SPE tubes ranging from 1-150 mL
in a wide range of bonded silica and polymeric chemistries, sorbent
particle sizes and bed masses.
For more specialized applications, the Luer compatible Bond Elut Jr
and the funnel-shaped large reservoir capacity (LRC) tube offer flexibility
and function in a broad range of sorbent bed masses.
Diagrams are to scale
60 mL 20 mL 12 mL

Sample Preparation
Bond Elut 96-well Plates

Bond Elut 96-well plate formats are best in class for flow performance and well-to-well
reproducibility. These specially designed plates are available with well depths of 1 mL
and 2 mL and in a large range of different sorbent chemistries.
VersaPlate
VersaPlate is a highly innovative, flexible design that lets you customize plates.
Insert different phases for sorbent screening or insert only enough tubes to match
the number of samples to be extracted for minimal waste. VersaPlate can be
purchased in a pre-packed format or as loose tubes.
Packed Formats for Automation

Bond Elut sorbents are also available in packed bed formats for automation platforms, such as
the Spark Holland Symbiosis, Gilson ASPEC and Gerstel MPS systems. Agilent's unique OMIX
pipette format is also used with a wide range of liquid handling devices, ranging from hand-held
pipettors to high-throughput automated systems.
10 mL LRC 6 mL 3 mL 1 mL Bond Elut Jr

Sample Preparation
Cross Reference of Comparable Phases by Manufacturer

Different chemistries and manufacturing processes create sorbents that exhibit differences in selectivity, so there is no universal equalivent for
every application. However, the performance of products can be similar in many applications. This table provides suggestions for using Agilent
Bond Elut products in comparison to products from other manufacturers.
Polymers
If you are using... Try this...
Phenomenex Waters Supelco Agilent
Strata Oasis Supelclean/Discovery Bond Elut
Strata-X HLB Plexa
SDB-L ENVI-ChromP ENV or LMS
Strata-XC MCX Plexa PCX
MAX Plexa PAX
Silica-Based and Other Sorbents

If you are using... Try this...
Phenomenex Waters Supelco Agilent
Strata Sep-Pak Supelclean/Discovery Bond Elut
C18-E tC18 ENVI-18, DSC-C18, LC-18 C18
C18-U C18 C18 OH
C8 C8 DSC-8, Envi-8, LC-8 C8
tC2 C2
Phenyl (PH) DSC-Ph, LC-Ph PH
Screen-C Certify
Si-1 Silica DSC-Si, LC-SI SI
FL-PR Florisil LC and ENVI Florisil FL
NH2 Amino Propyl DSC-NH2, LC-NH NH2
DSC-Diol, LC-Diol 2OH
CN Cyano Propyl DSC-CN, LC-CN CN-U
Alumina A, B, N LC-Alumina A, B, N Alumina A, B, N
SAX AccellPlus QMA DSC-SAX, LC-SAX SAX
SCX AccellPlus CM DSC-SCX, LC-SCX SCX
ENVI-Carb Carbon
ENVICarb-II/NH2 Carbon/NH2
ENVICarb-II/PSA Carbon/PSA

Sample Preparation
Sorbent Specifications
Typical
Carbon Surface Particle Size Mean
Bonded Functional Loading Area (µm) and Pore Size
Sorbent Phase Category Group/Base Material Endcapped Format (%) (m2/g) Shape (Å)
AccuCAT Mixed Mode Sulfonic acid (SCX) No Packed bed 7.0 500 40 and 120, 60
and quaternary amine irregular
(SAX) silica based
Alumina (AL-A) Polar Aluminium oxide – acidic Packed bed 0.0 25
Alumina (AL-B) Polar Aluminium oxide – basic Packed bed 0.0 25
Alumina (AL-N) Polar Aluminium oxide – neutral Packed bed 0.0 25
Aminopropyl (NH2) Polar/Anion Aminopropyl/silica based No Packed bed 6.7 500 40 and 120, 60
Exchanger irregular
SPEC Aminopropyl (NH2) Polar/Anion Aminopropyl/silica based No Monolithic disk 220 70
Exchanger
C1 Non-polar Methyl/silica based Yes Packed bed 4.1 500 40, irregular 60
C2 Non-polar Ethyl/silica based Yes Packed bed 5.6 500 40 and 120, 60
irregular
SPEC C2 Non-polar Dimethyl/silica based No Monolithic disk 2.7 220 70
C8 Non-polar Octyl/silica based Yes Packed bed 12.2 500 40 and 120, 60
irregular
SPEC C8 Non-polar Octyl/silica based Yes Monolithic disk 5.0 220
Carbon Strongly Graphitized carbon No Packed bed
Non-polar
C18 Non-polar Trifunctional Yes Packed bed 17.4 500 40 and 120, 60
octadecyl/silica based irregular
SPEC C18 Non-polar Monofunctional No Monolithic disk 8.0 220 70
octadecyl/silica based
SPEC C18 AR Non-polar Trifunctional Yes Monolithic disk 9.0 220 70
C18 EWP Non-polar Trifunctional Yes Packed bed 6.0 80 40, irregular 500
C18 INT Non-polar Trifunctional Yes Packed bed 13.0 500 40, irregular 60
C18 OH Non-polar Monofunctional No Packed bed 14.9 300 40 and 120, 150
octadecyl/silica based irregular
CBA Cation Carboxylic acid/silica based Yes Packed bed 7.4 500 40 and 120, 60
Exchanger irregular
(Continued)

Sample Preparation
Typical
Bonded Functional Loading Area (μm) and Pore Size
Certify Mixed Mode Octyl and benzenesulfonic No Packed bed 9.0 500 40 and 120, 60
acid (SCX)/silica based irregular
Certify II Mixed Mode Octyl and quaternary amine No Packed bed 8.6 500 40 and 120, 60
(SAX)/silica based irregular
CH Non-polar Cyclohexyl/silica based Yes Packed bed 9.6 500 40 and 120, 60
irregular
Cyano (CN-E) Non-polar Cyanopropyl/silica based Yes Packed bed 8.1 500 40 and 120, 60
irregular
SPEC Cyano Polar Cyanopropyl/silica based No Monolithic disk 220 70
SPEC DAU Application Silica based Monolithic disk 220 70
specific
DEA Anion Diethylaminopropyl/ No Packed bed 8.5 500 40 and 120, 60
Exchanger silica based irregular
Diol (2OH) Polar Diol/silica based No Packed bed 6.8 500 40, irregular 60
ENV Non-polar Styrene divinylbenzene Packed bed 125, spherical 450
EnvirElut 1664 Application Trifunctional No Packed bed 18.0 500 40 and 120, 60
specific octadecyl/silica based irregular
FL Polar Florisil Packed bed 200
Focus Polar Polar functionalized styrene Packed bed 640 44, spherical 120
enhanced divinylbenzene
LMS Non-polar Styrene divinylbenzene Packed bed 75, spherical 300
SPEC MP1 Mixed Mode Non-polar and Monolithic disk 6.0 220 70
benzenesulfonic acid
(SCX)/silica based
SPEC MP3 Mixed Mode Slightly polar and Monolithic disk 220 70
benzenesulfonic acid
(SCX)/silica based
NEXUS Mixed mode Mixed mode copolymer Packed bed 575 70, spherical 100/450
Bimodal
PBA Covalent Phenylboronic acid/ No Packed bed 7.9 500 40, irregular 60
silica based
PCB Application Layered phase Packed bed 500
specific
PH Non-polar Phenyl/silica based Yes Packed bed 10.7 500 40 and 120, 60
irregular
Plexa Polar Hydrophilic styrene Packed bed 550 45, spherical 100
enhanced divinylbenzene monodisperse
(Continued)

Sample Preparation
Typical
Bonded Functional Loading Area (μm) and Pore Size
Plexa PCX Cation Mixed SCX functionalized Packed bed 550 45, spherical 100
Mode hydrophilic styrene monodisperse
divinylbenzene
PPL Non-polar Functionalized styrene Packed bed 600 125, spherical 150
divinylbenzene
PRS Cation Propylsulfonic acid/ No Packed bed 1.7 500 40, irregular 60
Exchanger silica based
PSA Anion Ethylenediamine-N-propyl/ No Packed bed 7.5 500 40 and 120, 60
SPEC PSA Anion Ethylenediamine-N-propyl/ No SPEC disk 220 70
SPEC PH Non-polar Phenyl/silica based Yes Monolithic disk 220 70
SAX Anion Trimethylaminopropyl/ No Packed bed 7.5 500 40 and 120, 60
SPEC SAX Anion Trimethylaminopropyl/ No Monolithic disk 220 70
SCX Cation Benzenesulfonic acid/ No Packed bed 10.9 500 40 and 120, 60
SPEC SCX Cation Benzenesulfonic acid/ No Monolithic disk 220 70
SI Polar Silica No Packed bed 600 40 and 120, 60
irregular
SPEC SI Polar Silica No Monolithic disk 220 70
TCA Application Ethyl/silica based Yes Packed bed 500 40 and 120, 60
specific irregular
Particle Size Specifications

You will note that our most common silica-based Bond Elut packings are described as 40 μm materials, yet if you look at the actual lot analyses, you will see that
the actual mean is around 55 μm. We have been making silica-based Bond Elut packings since 1979, using the same diameter silicas; in that time, the models used
to estimate irregular particle "diameters" and the testing equipment have changed. We have retained the term "40 μm" however, because there are so many official
methods that specify a 40 μm Bond Elut sorbent. As other suppliers attempted to copy the successful Bond Elut product specifications, the term has become an industry
standard. You can be assured that the actual average particle in our regular silica Bond Elut is the same now as it was 30 years ago when we first pioneered SPE as
a sample prep technology.

Sample Preparation
Sample Preparation Reference Guide

Product Typical Matrices Primary Extraction Mechanism Compound Types Page No.
Bond Elut AccuCAT Urine, plasma and biological fluids, Strong cation and anion exchange Catecholamines, acrylamide 187
beverages and food in liquids and food
Bond Elut Alumina Non-polar organics Polar Polar cleanup 191
Bond Elut Atrazine Water sources Non-polar Atrazine and atrazine by-products 196
Bond Elut C1 Urine, plasma, biological fluids Non-polar, polar Strongly non-polar compounds 173
(as a normal phase extraction)
Bond Elut C18 Aqueous samples, biological fluids Non-polar Non-polar compounds, desalting 166
Bond Elut C18 Aqueous samples, biological fluids Non-polar Extra wide pore for larger, 168
EWP macro molecules up to 15 kDa
Bond Elut C18 OH Aqueous samples, biological fluids, Non-polar, hydrogen bonding Vitamin D, fat-soluble compounds, 169
non-polar extracts steroids/hormones
Bond Elut C2 Aqueous samples, biological fluids Non-polar Strongly non-polar compounds 174
Bond Elut C8 Aqueous samples, biological fluids Non-polar Non-polar compounds 170
Bond Elut CBA Aqueous samples, biological fluids Weak cation exchange Strong and weak bases 185
Bond Elut Cellulose Aqueous and non-polar organics Polar (Hydroxyl) Polar impurities/compounds 196
Bond Elut CH Aqueous samples, biological fluids Non-polar Non-polar compounds 172
(cyclohexyl)
Bond Elut CN-E Aqueous samples, biological fluids Non-polar, dipole Mid-range polarity compounds 176
Bond Elut Carbon Organic plant and tissue extracts Wide range non-polar retention Cleanup of pigments and 193
endogenous plant extracts
for pesticide and herbicide analysis
Bond Elut Certify Urine, plasma, saliva, blood, Non-polar and strong Basic drugs, basic drugs of abuse 188
biological fluids cation exchange
Bond Elut Certify II Urine, plasma, saliva, blood, Non-polar and strong anion exchange Acidic drugs, acidic drugs of abuse 189
biological fluids
Bond Elut DEA Water, biological fluids, Weak anion exchange Weak and strong acidic compounds 186
non-polar extracts
Bond Elut Diol Aqueous, biological fluids, Polar and non-polar Polar, weakly non-polar 177
(2OH) non-polar organics
Bond Elut ENV Water sources Non-polar Polar organic molecules, 163
explosive residues
Bond Elut Florisil Non-polar organics Polar compounds Organic extracts, non-polar 190
environmental extracts
Bond Elut LMS Urine, plasma, biological fluids Non-polar Non-polar compounds 164
(Continued)

Sample Preparation
Sample Preparation Reference Guide

Product Typical Matrices Primary Extraction Mechanism Compound Types Page No.
Bond Elut Aqueous and polar organic grain Ionic cleanup Mycotoxins (trichothecenes 198
Mycotoxin extracts (beer, wine, sake) and zearalenones)
Bond Elut NEXUS Horse urine, urine, biological fluids Non-polar Drugs of abuse, quaternary drugs, 165
and Bond Elut endocrine disruptors
NEXUS WCX
Bond Elut NH2 Aqueous, biological fluids, buffered Weak anion exchange Polar and non-polar strong anions, 178
organics polar structural isomers
Bond Elut PBA Plasma, urine, aqueous Covalent bonding cis-diol-containing compounds, 200
and biological fluids catecholamines, ribonucleotides,
amino alcohols, diketo and triketo
compounds
Bond Elut PCB Water sources Non-polar PCBs 197
Bond Elut PH Aqueous and biological fluids Non-polar Strongly non-polar compounds, 171
aromatics
Bond Elut PPL Water sources, biological fluids Non-polar, electrostatic Non-polar compounds, phenols 161
Bond Elut PRS Aqueous, biological fluids, Cation exchange Basic compounds 183
buffered organics (amine + pyridinium containing)
Bond Elut PSA Aqueous, biological fluids, Strong anion exchange Acidic compounds 184
buffered organics (fruit acid removal for QuEChERS)
Bond Elut Plexa Aqueous, biological fluids Non-polar Non-polar compounds with 156
acidic/neutral fractionation
PAHs from water
Bond Elut Plexa Plasma, urine, aqueous Mixed mode: non-polar Acidic compounds, carboxylic acid 160
PAX and biological fluids and anion exchange metabolites of drugs, peptides
and amino acids
Bond Elut Plexa Aqueous, biological fluids, Mixed mode: non-polar and cation Basic drugs, basic drugs of abuse 158
PCX buffered organics exchange
Bond Elut SAX Aqueous, biological fluids Anion exchange Weak acidic compounds 180
Bond Elut SCX Aqueous, biological fluids, Cation exchange Weak basic compounds 182
buffered organics
Bond Elut SI Non-polar organics, oils, lipids Polar Cleanup of polar impurities 175
EnvirElut Water sources, extracted soil samples Non-polar Pesticide and industrial 201
chemical residues
Chem Elut Aqueous, biological fluids, organic Solid supported LLE Nitrosamines, pesticides, herbicides 202
reaction mixtures (scavenging)
Hydromatrix Aqueous, biolgoical fluids, organic Solid supported LLE Nitrosamines, pesticides, herbicides 202
reaction mixtures (scavenging)

Bond Elut Plexa
Bond Elut Plexa

The Bond Elut Plexa Family is a new generation of polymeric SPE products, designed for
simplicity, improved analytical performance and ease-of-use. Its uniqueness lies in the novel
hydroxylated exterior, hydrophobic interior and advanced polymeric architecture.
Bond Elut Plexa

Bond Elut Plexa is a non-polar divinylbenzene-based neutral polymeric sorbent. This sorbent is
the best choice for non-ionic extraction of a wide range of acidic, neutral and basic analytes from
different matrices.
Bond Elut Plexa PCX

Bond Elut Plexa PCX is a cation exchanger with mixed mode sorbent characteristics and is
therefore suitable for the extraction and cleanup of weak bases from biofluids. Bond Elut Plexa PCX
demonstrates the same excellent particle size distribution and integrity as Bond Elut Plexa. A highly
controlled sulfonation process results in zero fines for Bond Elut Plexa PCX.
Bond Elut Plexa PAX

Bond Elut Plexa PAX is based on the same innovative base polymer particle technology
as the other members of the Plexa SPE family. This advanced material offers excellent flow
characteristics due to its monodisperse particle size distribution, affording superior ease-of-use,
with minimal clogging of the packed bed. The amide-free particle technology does not provide
binding sites for endogenous interferences such as proteins and lipids.

Bond Elut Plexa
Advanced Polymer Architecture Improves Extraction Performance
LOAD: WASH: ELUTE:

Water-rich, hydrophilic surface allows excellent Analytes that have crossed the Specially engineered pore structure allows
phase transfer of analytes into the polymer core. hydrophilic layers will remain tightly excellent mass transfer out of the polymer.
bound in the hydrophobic core.
Analyte
Pores
Large endogenous proteins do not bind to Interferences wash away without leaching Clean extract with high recovery.
the surface of the polymer and cannot access the analytes of interest.
pore structure.
Tips & Tools

More information is a click away. We have a variety
of educational primers, application notes, maintenance
guides, and literature available from Agilent for free.
To learn more, visit www.agilent.com/chem/OnlineLibrary

Bond Elut Plexa
General Protocol for Trouble-Free SPE Applications

with Bond Elut Plexa
Regardless of your application or sample type, you will appreciate the difference the
Bond Elut Plexa range makes. Plexa delivers simple methods, superior flow characteristics,
and improved analytical performance, all leading to easier validation. Simple methods deliver
clean extracts and high recoveries from a wide range of acidic, basic and neutral analytes.
The advanced polymeric design effectively eliminates the common matrix interferences
that cause ion suppression, resulting in improved analytical sensitivity and data quality.
Acids Neutrals Bases
Log P > 1.0 Log P > 1.5 Log P > 0.8

Analyte
pKa > 3 pKa 3-6 pKa 6-10 pKa 6-10
Plexa Acid Plexa Base

Plexa PAX Plexa PCX
Load Method Load Method
Sample Treatment 2% NH4OH 1% HCO2H 2% NH4OH 2% H3PO4
Sorbent Condition 100% MeOH 100% MeOH 100% MeOH
Equilibrate 100% H2O 100% H2O 100% H2O
Wash 100% H2O 5% MeOH in H2O 2% HCO2H in H20
Elution 1 100% MeOH 100% MeOH 1:1 MeOH/ACN

Neutrals Neutrals Acids, Neutrals
5% HCO2H 5% NH3
Elution 2 in MeOH in 1:1 MeOH/ACN
Bases Bases

Bond Elut Plexa
Improved Sensitivity
Matrix interferences can result in significantly decreased analytical sensitivity due to ion
Plexa improves sensitivity suppression. Bond Elut Plexa gives you higher recoveries in cleaner extracts, which translates
by minimizing ion suppression into better sensitivity. Plexa delivers high recoveries regardless of whether absolute or relative
effects and maximizing recovery calculations are used. This indicates that ion suppression is minimized and maximum sensitivity
is achieved. Relative recovery calculations (green bars) are routinely used, but mask the effects
of ion suppression, which are normalized.
Bond Elut General Cation
Plexa purpose exchange
polymer X polymer X
120
Comparison of particle sizes of non-polar SPE polymers

% Recovery
by imaging analysis
Key
Metoprolol recovery calculated as

response against spiked mobile phase
Metoprolol recovery calculated as Bond Elut Plexa PCX Alternative Polymer

response against an extracted
linear curve
Comparison of particle size distributions of non-polar SPE sorbents

Bond Elut Plexa PCX Other CX resin
Plexa PCX Other CX Polymer
Plexa Other Polymer
The narrow particle size distribution offers reproducible, superior flow characteristics with minimal clogging

Bond Elut Plexa
Bond Elut Plexa

• Non-polar retention mechanism
• Improved extract cleanliness minimizes sample matrix interferences
• Simple methods are amenable to a very broad range of analytes
• Fast flow, reproducible performance and ease-of-use
Bond Elut Plexa offers simple, easy-to-use methods with general purpose extraction
mechanisms to simplify SPE. In addition, Plexa provides performance enhancements due to
a unique polymeric architecture with a non-retentive, hydroxylated, amide-free surface and
a non-polar PS/DVB core for retaining small molecules. Binding of proteins and lipids on the
polymer surface is minimized, resulting in cleaner samples and reduced ion suppression.
Plexa is therefore ideal for high-throughput assays requiring validated performance with
minimal method development. The standard non-polar retention mechanism is applicable
to almost any analyte type, and the performance features operate at the sample loading
step, making them largely method independent.
By minimizing the need for extensive method development for multiple sorbents,
Bond Elut Plexa simplifies SPE. The water wettable, hydroxylated exterior allows excellent
flow of biofluid samples. A gradient of polarity on the polymer surface shunts small analytes
to the more hydrophobic center of the polymer bead where they are retained prior to washing
and elutions steps.
Typical Matrices Primary Extraction Mechanism Compound Types

Aqueous, Non-polar Non-polar compounds
biological fluids with acidic/neutral
fractionation
PAHs from water

Bond Elut Plexa
Bond Elut Plexa

Description Unit Part No.
Straight Barrel Cartridges
30 mg, 1 mL 100/pk 12109301
30 mg, 3 mL 50/pk 12109303
60 mg, 1 mL 100/pk 12109601
60 mg, 3 mL 50/pk 12109603
200 mg, 3 mL 50/pk 12109610
200 mg, 6 mL 30/pk 12109206
500 mg, 3 mL 30/pk 12109703
500 mg, 6 mL 30/pk 12259506
Bond Elut Jr
300 mg, 6 mL 50/pk 12169610B
Bond Elut 96 Round-well Plates
10 mg, 1 mL 1/pk A4969010
30 mg, 1 mL 1/pk A4969030
Bond Elut 96 Square-well Plates
10 mg, 2 mL 1/pk A3969010
30 mg, 2 mL 1/pk A3969030
Mega Bond Elut Plexa
500 mg, 12 mL 20/pk 327832
Other Formats
Bond Elut Plexa Prospekt cartridge, 2 mm 96/pk 12221305
Bond Elut Plexa 800 Series cartridge 96/pk 12281305
60 mg, 3 mL, Gerstel format 50/pk 167816G

Bond Elut Plexa
Bond Elut Plexa PCX

Typical Method
for Bond Elut Plexa PCX • Faster flow rates improve productivity
• Extraction cleanliness and reduced ion suppression improve precision
Sample:
• Simplified, single method for ease-of-use
100 μL plasma Bond Elut Plexa PCX is another milestone in the development of simple and robust SPE
methods. Plexa PCX uses a polymeric cation exchange resin that combines the outstanding
Pretreatment: properties of Bond Elut Plexa – superior flow characteristics and improved analytical
Dilute 1:3 with 2% H3PO4 performance – with strong cation exchange functionalities. This mixed-mode SPE sorbent
removes neutral and acidic interferences from the matrix, concentrates basic analytes
Conditioning: and therefore improves sensitivity in the determination of basic compounds.
1. 500 μL MeOH
The Plexa PCX particles are near mono-dispersed, resulting in homogenous packing.
2. 500 μL H2O
Reproducible results are the norm, with very good tube-to-tube and well-to-well performance.
Washes: Ion suppression is reduced because the highly polar, hydroxylated polymer surface is entirely
Acidic wash: 500 μL aqueous 2% amide-free and does not provide binding sites for endogenous species such as proteins
formic acid and lipids.
Neutral wash: 500 μL CH3OH-CH3CN Plexa PCX comes with a simple, single method approach for basic drugs that offers improved
(1:1, v/v) recoveries, cleaner extracts and reduced method development time and cost. Flow rate is
improved because Plexa PCX particles have much narrower particle size distribution with
Elution:
no fines to cause blockages.
500 μL CH3OH-CH3CN + 5% NH3
(28-30%)
Volumes stated are for Bond Elut 96 Aqueous, biological Mixed mode: non-polar and cation exchange Basic drugs, basic
30 mg, 1 mL, P/N A4968030. fluids, buffered drugs of abuse
organics

Bond Elut Plexa
Bond Elut Plexa PCX

30 mg, 1 mL 100/pk 12108301
60 mg, 1 mL 100/pk 12108601
30 mg, 3 mL 50/pk 12108303
60 mg, 3 mL 50/pk 12108603
200 mg, 6 mL 30/pk 12108206
500 mg, 6 mL 30/pk 12258506
10 mg, 1 mL 1/pk A4968010
30 mg, 1 mL 1/pk A4968030
10 mg, 2 mL 1/pk A3968010
30 mg, 2 mL 1/pk A3968030
Other Formats
Bond Elut Plexa PCX Prospekt Cartridge, 2 mm 96/pk 12221306
Bond Elut Plexa PCX 800 Series Cartridge 96/pk 12281306
Tips & Tools

Don't forget, we have special offers throughout the year.
To learn more, visit www.agilent.com/chem/specialoffers

Bond Elut Plexa
Bond Elut Plexa PAX

Typical Method
for Bond Elut Plexa PAX • Mixed mode, non-polar polymeric anion exchanger offers high level of analyte selectivity
• Exclusion of endogenous interferences offers superior cleanliness and minimizes
ion suppression
Sample:
• Simple, single method for ease-of-use, reduces method development time
100 µL human plasma
Bond Elut Plexa PAX sets the new performance standard in analyte cleanup and reproducibility
Pretreatment:
for polar and non-polar acidic analytes. Existing polymeric anion exchange sorbents can exhibit
Dilute 1:3 with 2% NH4OH a broad range of ion exchange capacity from batch to batch, leading to method irreproducibility
Conditioning:
and compromised data. Plexa PAX particles are functionalized using a proprietary process
which allows anion exchange loadings to be controlled with a very high degree of reproducibility,
1. 500 µL MeOH
giving more robust performance across the lifetime of your compound study or method.
2. 500 µL H2O
This mixed-mode SPE device comes with a simple, single method for non-polar acidic and polar
Washes:
acidic analytes that offers excellent clean up, even in complex matrices such as plasma. The
1. 500 µL H2O
optimized anion exchange methodology offers clean extracts, high recoveries and low RSDs,
2. 500 µL MeOH
reducing method development time, sample repeats and overall cost per sample in the process.
Elution:
500 µL 5% formic acid:MeOH Typical Matrices Primary Extraction Mechanism Compound Types
Plasma, urine, aqueous Mixed mode: non-polar and anion exchange Acidic compounds,
Volumes stated are for Bond Elut 96 and biologocal fluids carboxylic acid
1 mL Well Plate, P/N A4967010. metabolites of drugs,
peptides and
amino acids
Bond Elut Plexa PAX

30 mg, 1 mL 100/pk 12107301
60 mg, 1 mL 100/pk 12107601
30 mg, 3 mL 50/pk 12107303
60 mg, 3 mL 50/pk 12107603
200 mg, 6 mL 30/pk 12107206
500 mg, 6 mL 30/pk 12257506
10 mg, 1 mL 1/pk A4967010
30 mg, 1 mL 1/pk A4967030
10 mg, 2 mL 1/pk A3967010
30 mg, 2 mL 1/pk A3967030

Polymeric SPE
Polymeric SPE
Reversed Phase Polymeric SPE
Bond Elut PPL
• Modified styrene-divinylbenzene polymer
• Large particle size allows fast extraction speeds
• High surface area and capacity for polar analytes
Bond Elut PPL is a styrene-divinylbenzene (SDVB) polymer that has been modified with
a proprietary non-polar surface. PPL will retain even the most polar classes of analytes,
including phenols. The large particle size allows ease of flow for viscous or particulate rich
water samples, while the high surface area and strong hydrophobicity ensure reproducible
extractions with high recoveries upon elution.
Bond Elut PPL is suitable for EPA Method 528 'Determination of Phenols in Drinking Water
by SPE and Capillary GC/MS.'

Water sources, Non-polar, electrostatic Non-polar compounds,
biological fluids phenols

Polymeric SPE
Bond Elut PPL

50 mg, 1 mL 100/pk 12105002
100 mg, 1 mL 100/pk 12105003
100 mg, 3 mL 50/pk 12105004
200 mg, 3 mL 50/pk 12105005
500 mg, 3 mL 50/pk 12105006
500 mg, 6 mL 30/pk 12255001
1 g, 3 mL 50/pk 12102148
1 g, 6 mL 30/pk 12255002
Determination of organophosphates in lake water
Vacuum Manifold: Vac Elut 20 Method: 1. Apply 1.5-2.5 L water sample

2. Dry the cartridge using nitrogen
Vacuum: 800 mbar 3. Elution with 3 x 333 μL methanol/acetonitrile (1/1)
Cartridge: Bond Elut PPL, 100 mg sorbent
in 1 mL cartridge Analyte Recovery (%) LOD (ng/L)
Condition cartridge with 1 mL Tris (1-chloro-2-propyl)-phosphate (TCPP) 91 1
methanol, 1 mL ethanol/
acetonitrile (1/1) Tris (2-chloroethyl)-phosphate (TCEP) 95 2
Tris (1,3-dichloro-2-propyl)-phosphate (TDCP) 99 1
Tri-n-butylphosphate (TnBP) 89 1
Recoveries and LODs of organophosphates; extracted from Tri-isobutylphosphate (TiBP) 85 2
the water sample with SPE Tris(2-butoxyethyl)-phosphate (TBEP) 93 3
Courtesy: Application Note SI-02094 Determination of Organophosphates in Lake Water

Polymeric SPE
Bond Elut ENV

• Modified styrene-divinylbenzene polymer
• Large particle size allows fast extraction speeds
• High surface area and capacity for polar analytes
Bond Elut ENV, a PS/DVB polymer, is designed for the extraction of polar organic residues.
It contains 125 μm spherical particles, advantageous for high volume, fast flow-through applications.

Water sources Non-polar Polar organic
molecules,
explosive residues
Bond Elut ENV

50 mg, 1 mL 100/pk 12105012
100 mg, 1 mL 100/pk 12105013
100 mg, 3 mL 50/pk 12105014
200 mg, 3 mL 50/pk 12105015
200 mg, 6 mL 30/pk 12255014
500 mg, 3 mL 50/pk 12105016
500 mg, 6 mL 30/pk 12255011
1 g, 6 mL 30/pk 12255012
Extraction of explosive residues from water
Sorbent Conditioning: 200 mg/3 mL Bond Elut ENV cartridge Compounds Recoveries (%)
Apply Sample: Adjust 500 mL sample to pH 2 using concentrated HCl 1,3,5-Trinitrobenzene 99.8
Nitrobenzene 92.1
Interference Wash: 500 mL of water sample at a flow rate 2,4-Dinitrotoluene 97.7
between 10 and 15 mL/min.
2,6-Dinitrotoluene 86.8
Analyte Elution: 5 mL DI H2O, then dry the cartridge for 3 min 2-Amino-4,6-dinitrotoluene 93.2
4-Amino-2,6-dinitrotoluene 93.3
1. 2.5 mL ACN (2 mL of which re-eluted x 4
after 1st elution) 4-Nitrotoluene 85.3
2. 1.5 mL fresh ACN

Polymeric SPE
Bond Elut LMS

• Ultra clean styrene-divinylbenzene polymer
• Optimized 75 μm particle size for reproducible flow
• High capacity and surface area for efficient extraction
Bond Elut LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers,
or acids. The elimination of secondary interactions means that elution of analytes can be
achieved with pure organic solvents or solvent mixtures of low ionic strength compatible
with the HPLC mobile phase. These characteristics are crucial to allow compatibility with
LC/MS or other delicate analytical techniques.

Urine, plasma, Non-polar Non-polar compounds
biological fluids
Bond Elut LMS

25 mg, 1 mL 100/pk 12105021
100 mg, 1 mL 100/pk 12105023
100 mg, 3 mL 50/pk 12105024
200 mg, 3 mL 50/pk 12105025
500 mg, 3 mL 50/pk 12105026
500 mg, 6 mL 30/pk 12255021
1 g, 6 mL 30/pk 12255022
Tips & Tools

Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

Polymeric SPE
Mixed Mode Polymeric SPE

Bond Elut NEXUS and Bond Elut NEXUS WCX
• Large particle size allows excellent flow for viscous samples
• Non-conditioning method saves time and improves throughput
• WCX offers enhanced selectivity for certain analytes such as quaternary amine drugs
Bond Elut NEXUS is an ultra-clean polymeric sorbent which has bi-modal porosity and a high
surface area. NEXUS offers a non-polar retention mechanism with no pre-conditioning required.
The large particle size makes NEXUS ideal for extractions from highly viscous samples such
as horse urine.
References
Wynne, PM, Barry, DC, Vine, JH & Simpson, NKJ Based on the same base polymer technology, Bond Elut NEXUS WCX is a weak cation exchange
(2004) Approaches to the solid phase extraction of
equine urine. Chromatography, 59, S51-S60.
sorbent that offers extra selectivity for analytes such as quaternary ammonium drugs and
Wynne, PM, Barry, DC, Vine, JH & Simpson, NKJ
anabolic steroids.
(2000) An improved method for the extraction of
anabolic steroids from equine urine. In: RB Williams,
E Houghton & J Wade (eds) Proc. 13th Int. Conf. Typical Matrices Primary Extraction Mechanism Compound Types
Racing Analysts and Veterinarians. R & W
Publications, Newmarket, UK. Horse urine, urine, Non-polar Drugs of abuse,
biological fluids quaternary drugs,
endocrine disruptors
Bond Elut NEXUS and Bond Elut NEXUS WCX

LRC Cartridges
30 mg, 10 mL 50/pk 12113100
60 mg, 10 mL 50/pk 12113101
30 mg, 1 mL 100/pk 12103100
60 mg, 3 mL 100/pk 12103101
60 mg, 3 mL, NEXUS WCX 100/pk 12102157
200 mg, 6 mL 30/pk 12103102
200 mg, 12 mL 20/pk 12253101
500 mg, 12 mL 20/pk 12253102
500 mg, 20 mL 20/pk 12253103

Silica-Based SPE
Silica-Based SPE
Reversed Phase (Non-Polar) Silica SPE
Reversed phase sorbents are non-polar and are used to retain (extract) non-polar analytes from
polar matrices. For reversed phase sorbents, retention decreases as the eluting solvent becomes
more non-polar.
Bond Elut C18

CH3 • The most hydrophobic, bonded silica sorbent
• Extremely retentive for non-polar compounds
O Si (CH2)17 CH3 • Effective for desalting aqueous mixtures
CH3 Bond Elut C18 is the most hydrophobic, bonded silica sorbent in the Bond Elut range. It is the
most popular SPE sorbent because of its extremely retentive nature for non-polar compounds.
C18 is generally regarded as having the broadest spectrum of retention among bonded silica
sorbents, since it retains most organic analytes from aqueous matrices. When analyzing small to
intermediate molecules, Bond Elut C18 can be used for desalting aqueous matrices prior to ion
exchange, as salts pass through the sorbent unretained.

Aqueous samples, Non-polar Non-polar compounds,
biological fluids desalting

Silica-Based SPE
Bond Elut C18

40 μm 120 μm
Description Unit Particle Size Particle Size
LRC Cartridges
100 mg, 10 mL 50/pk 12113001 14113001
200 mg, 10 mL 50/pk 12113024 14113024
500 mg, 10 mL 50/pk 12113027 14113027
50 mg, 1 mL 100/pk 12102058 14102058
50 mg, 3 mL 50/pk 12105027
100 mg, 1 mL 100/pk 12102001 14102001
100 mg, 3 mL 50/pk 12102099
200 mg, 1 mL 100/pk 12102096
200 mg, 3 mL 50/pk 12102025 14102025
500 mg, 3 mL 50/pk 12102028 14102028
500 mg, 6 mL 30/pk 12102052 14102052
1 g, 3 mL 50/pk 12102118
1 g, 6 mL 30/pk 12256001 14256001
1 g, 60 mL 16/pk 12256060
2 g, 12 mL 20/pk 14256015
5 g, 20 mL 20/pk 14256023
10 g, 60 mL 16/pk 14256031
Bond Elut Jr
500 mg 100/pk 12162028B
1g 100/pk 12166001B
Other Formats
Prospekt cartridge, 800 Series 96/pk 12281001
Prospekt cartridge, 800 Series, 1 mm 96/pk 12281024
VersaPlate Formats
Particle Size
Description (μm) 25 mg 50 mg 100 mg
Preassembled 96-well plate 40 75401050 7540101C
VersaPlate tubes, 96/pk 40 75501025 75501050 7550101C
120 75502050

Silica-Based SPE
Bond Elut C18 EWP

CH3 • No exclusion of large molecules
• Good for desalting proteins
O Si (CH2)17 CH3 • Successful separation of proteins, peptides or nucleotides
CH3
Bond Elut EWP is based on standard particle size silica but with 500Å pores to allow more
efficient extraction of large molecules (>15,000 MW), which are typically excluded from
standard porosity silica phases.

Aqueous samples, Non-polar Extra wide pore
biological fluids for larger, macro
molecules up to 15 kDa
Bond Elut C18 EWP

LRC Cartridges
50 mg, 10 mL 50/pk 12113068
500 mg, 10 mL 50/pk 12113071
50 mg, 1 mL 100/pk 12102136
100 mg, 1 mL 100/pk 12102137
500 mg, 3 mL 50/pk 12102139

Silica-Based SPE
Bond Elut C18 OH

CH3 • Silanol activity permits metabolite fractionation
• Tight QC tolerances deliver batch-to-batch reproducibility
O Si (CH2)17 CH3 • 150Å pore size expands utility to higher molecular weight compounds
CH3 Bond Elut C18 OH is a non-endcapped version of the octadecyl bonded phases that enables
the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol
activity that permits the fractionation of metabolites and enhances retention of basic compounds
compared to an endcapped C18.

Aqueous samples, Non-polar, hydrogen bonding Vitamin D, fat-soluble
biological fluids, compounds,
non-polar extracts steroids/hormones
Bond Elut C18 OH

100 mg, 1 mL 100/pk 12102020
500 mg, 3 mL 50/pk 12102046
1 g, 6 mL 30/pk 12256040

Silica-Based SPE
Bond Elut C8
CH3 • Excellent for strongly-retained analytes
• Polar interactions not significant
O Si (CH2)7 CH3 • Less retentive than C18
CH3 Bond Elut C8 is very similar in property to C18, but is not as retentive for non-polar compounds,
due to its shorter hydrocarbon chain, and therefore reduced carbon loading. C8 is an excellent
replacement for C18 when analytes are too strongly retained for effective elution. The potential
for polar interactions is somewhat higher than for C18 because there is less coverage of the
silica surface. These polar interactions are not, however, a significant property of C8.

Aqueous samples, Non-polar Non-polar compounds
biological fluids
Bond Elut C8
Bond Elut Jr
500 mg 100/pk 12162029B
LRC Cartridges
100 mg, 10 mL 50/pk 12113002
200 mg, 10 mL 50/pk 12113025
500 mg, 10 mL 50/pk 12113028
50 mg, 1 mL 100/pk 12102059
50 mg, 3 mL 50/pk 12105028
100 mg, 1 mL 100/pk 12102002
100 mg, 3 mL 50/pk 12102100
200 mg, 3 mL 50/pk 12102026
500 mg, 3 mL 50/pk 12102029
500 mg, 6 mL 30/pk 12102053
1 g, 6 mL 30/pk 12256002
5 g, 20 mL 20/pk 12256024
10 g, 60 mL 16/pk 12256032
(Continued)

Silica-Based SPE
Bond Elut C8
Other Formats
Prospekt cartridge, 800 Series, 1 mm 96/pk 12281025
VersaPlate Formats
Description Particle Size (μm) 50 mg 100 mg
Preassembled 96-well plate 40 7540301C
VersaPlate tubes, 96/pk 40 75503050 7550301C
Bond Elut PH
CH3 • Added selectivity compared to other non-polar sorbents
• Enhanced retention of planar, conjugated organic molecules
O Si • Similar polarity to C8
CH3 Bond Elut PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl
or aliphatic functionalized phases such as C8 or cyclohexyl. The electron density present in the
aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing
analytes due to desirable pi-pi interactions.

Aqueous and Non-polar Strongly non-polar
biological fluids compounds, aromatics
Bond Elut PH
40 μm 120 μm
LRC Cartridges
100 mg, 10 mL 50/pk 12113005 14113005
500 mg, 10 mL 50/pk 12113031 14113031
50 mg, 1 mL 100/pk 12102062 14102062
100 mg, 1 mL 100/pk 12102005 14102005
500 mg, 3 mL 50/pk 12102032 14102032
1 g, 6 mL 30/pk 12256004 14256004

Silica-Based SPE
Bond Elut CH (cyclohexyl)

CH3 • Non-polar CH with polarity similar to C2
• Retains polar analytes from aqueous matrices
O Si • Good choice when common non-polar sorbents do not provide the required selectivity
CH3 Bond Elut CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When
employed as a non-polar sorbent, CH has the approximate polarity of a C2 sorbent. Bond Elut CH
is often a good choice when non-polar sorbents such as C18, C8, or C2 do not provide the
desired selectivity.

Aqueous samples, Non-polar Non-polar compounds
biological fluids
Bond Elut CH (cyclohexyl)

LRC Cartridges
500 mg, 10 mL 50/pk 12113032
50 mg, 1 mL 100/pk 12102063
100 mg, 1 mL 100/pk 12102006
500 mg, 3 mL 50/pk 12102033
1 g, 6 mL 30/pk 12256005
2 g, 12 mL 20/pk 12256039

Silica-Based SPE
Bond Elut C1
CH3 • Least retentive of all alkyl group bonded phases
• Easy retention and release of polar compounds
O Si CH3 • Easy retention and release of multi-functional compounds
CH3 Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive
of all alkyl group bonded phases for non-polar compounds. However, due to the extensive
endcapping of this sorbent to mask polar silanol activity, retention and elution of polar
and multi-functional analytes can still be achieved.

Urine, plasma, Non-polar, polar (as a normal phase extraction) Strongly non-polar
biological fluids compounds
Bond Elut C1
LRC Cartridges
100 mg, 10 mL 50/pk 12113004
300 mg, 10 mL 50/pk 12113053
500 mg, 10 mL 50/pk 12113030
50 mg, 1 mL 100/pk 12102061
100 mg, 1 mL 100/pk 12102004
100 mg, 3 mL 50/pk 12102090
500 mg, 3 mL 50/pk 12102031

Silica-Based SPE
Bond Elut C2
CH3 • Low carbon load sorbent
• Can be used alongside CN and C8 phases
O Si CH2 CH3 • Popular for drug extraction from plasma and for flat baselines
CH3 Bond Elut C2 is a fairly non-polar sorbent because of the short chain length of the functional
group. C2 is often used during the process of method development if analytes are retained
too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase
for polar interactions.

Aqueous samples, Non-polar Strongly non-polar
Bond Elut C2
50 mg, 1 mL 100/pk 12102060
50 mg, 3 mL 50/pk 12105029
100 mg, 1 mL 100/pk 12102003
100 mg, 3 mL 50/pk 12102117
200 mg, 3 mL 50/pk 12102027
500 mg, 3 mL 50/pk 12102030
500 mg, 6 mL 30/pk 12102115
1 g, 6 mL 30/pk 12256003

Silica-Based SPE
Normal Phase (Polar) Silica SPE

Normal phase sorbents are polar and used to retain (extract) polar analytes. For normal phase
sorbents, retention decreases as the eluting solvent becomes more polar.
Bond Elut SI
CH3 • Highly polar phase retains polar molecules from non-polar matrices
• High purity silica
O Si OH • Separate compounds with very similar structures
CH3 Native silica is generally regarded as the most polar SPE sorbent available. Bond Elut SI is
particularly effective at separating compounds with a very similar structure. Applying the analytes
in a non-polar solvent, then increasing the solvent polarity by increasing the concentration of
a polar modifier, such as THF or ethyl acetate, delivers effective separations.

Non-polar organics, Polar Cleanup of polar
oils, lipids impurities
Bond Elut SI
40 μm 120 μm
LRC Cartridges
100 mg, 10 mL 50/pk 12113010 14113010
500 mg, 10 mL 50/pk 12113036 14113036
50 mg, 1 mL 100/pk 12102068 14102068
100 mg, 1 mL 100/pk 12102010 14102010
500 mg, 3 mL 50/pk 12102037 14102037
1 g, 6 mL 30/pk 12256008 14256008
1.5 g, 3 mL 50/pk 12102119
2 g, 6 mL 30/pk 14256018
5 g, 20 mL 20/pk 14256026
10 g, 60 mL 16/pk 14256034
Bond Elut Jr
500 mg 100/pk 12162037B
1g 100/pk 12166008B
Other Formats

Silica-Based SPE
Bond Elut CN-E

CH3 • Ideal for extracting aqueous analytes
• Retention in aqueous and organic matrices
O Si (CH2)3 CN • Useful for many applications
CH3 A medium polarity sorbent with many uses, Bond Elut CN-E is ideal for applications in which
extremely non-polar compounds would be irreversibly retained on high carbon load sorbents
such as C8 and C18. This endcapped version of the cyano sorbent is best utilized when
extracting analytes from an aqueous matrix.
References
Pucci, V, Bugamelli, F, Mandrioli, R, Bartoletti,
C, Rossi, N & Raggi, MA (2003) Liquid Aqueous samples, Non-polar, dipole Mid-range polarity
chromatographic analysis of the cis(Z)- and biological fluids compounds
trans(E)-isomers of clopenthixol in human plasma
using a novel solid phase extraction procedure.
J. Chromatogr. B., 792, 313-321.
Bond Elut CN-E
LRC Cartridges
100 mg, 10 mL 50/pk 12113007
500 mg, 10 mL 50/pk 12113033
50 mg, 1 mL 100/pk 12102064
100 mg, 1 mL 100/pk 12102007
500 mg, 3 mL 50/pk 12102034
5 g, 20 mL 20/pk 12256025

Silica-Based SPE
Bond Elut Diol (2OH)

CH3 OH • Provides polar and non-polar modes
• Strong hydrogen bonding with analytes
O Si (CH2)3 O OH • Resembles un-bonded silica in its capabilities
CH3 Bond Elut Diol resembles un-bonded silica in its tendency for strong hydrogen bonding with
analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer
on its functional group provides enough non-polar character for retention of hydrophobic analytes.
Bond Elut Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.

Aqueous, biological Polar and non-polar Polar, weakly
fluids, non-polar non-polar
organics
Bond Elut Diol (2OH)

LRC Cartridges
100 mg, 10 mL 50/pk 12113009
500 mg, 10 mL 50/pk 12113035
50 mg, 1 mL 100/pk 12102067
100 mg, 1 mL 100/pk 12102009
500 mg, 3 mL 50/pk 12102036
1 g, 6 mL 30/pk 12256007

Silica-Based SPE
Bond Elut NH2

CH3 • Normal phase or anion exchange sorbent
• Weaker anion exchange than SAX
O Si (CH2)3 NH2 • Amenable to separating structural isomers
CH3 Bond Elut NH2 is a weaker anion exchanger than sorbents such as SAX (a quaternary amine
sorbent that is always charged) and is therefore a better choice for retention of very strong
anions, such as sulfonic acids, which may retain irreversibly on a SAX sorbent. Similar to Diol
and SI sorbents, Bond Elut NH2 is excellent for the separation of structural isomers.
References
Schenck, F, Lehotay, S, & Vega, V (2002)
Comparison of solid phase extraction sorbents Aqueous, biological Weak anion exchange Polar and non-polar
for cleanup of pesticide residue analysis in fresh fluids, buffered strong anions, polar
fruit and vegetables. J. Sep. Sci., 25, 883-890. organics structural isomers
Bond Elut NH2

40 μm 120 μm
LRC Cartridges
100 mg, 10 mL 50/pk 12113014 14113014
200 mg, 10 mL 50/pk 12113067
500 mg, 10 mL 50/pk 12113040 14113040
50 mg, 1 mL 100/pk 12102076 14102076
100 mg, 1 mL 100/pk 12102014
200 mg, 3 mL 50/pk 12102089
200 mg, 6 mL 30/pk 12102106
300 mg, 3 mL 50/pk 12102108
500 mg, 3 mL 50/pk 12102041 14102041
500 mg, 6 mL 30/pk 12256045
1 g, 3 mL 50/pk 12102107
1 g, 6 mL 30/pk 12256012 14256012
2 g, 12 mL 20/pk 14256020
5 g, 20 mL 20/pk 14256028
Bond Elut Jr
500 mg 50/pk 12162041B
1 g, 3 mL 50/pk 12166012B
Other Formats

Silica-Based SPE
VersaPlate Formats
The isolation of lipids from serum and tissue
Extraction Method
Matrix: Elution 1:
Chloroform extract of (Neutral lipids)
serum or adipose tissue
(All except fatty acids and phospholipids) – 2:1 chloroform:2 propanol
Sorbent Conditioning:
(Fatty acids)
Hexane
2% acetic acid in diethyl ether
Apply Sample:
(Phospholipids)
Through Bond Elut NH2
Methanol
cartridge
The neutral lipid fraction is then dried down, reconstituted in hexane, and passed through
a second NH2 tube conditioned with hexane.
Elution 2:
(Cholesterol esters)
Hexane
Another Bond Elut NH2 sorbent column is attached below the existing one to trap
cholesterol that breaks through the first during triglyceride elution.
Elution 3:
(Triglycerides)
Hexane containing 1% diethyl ether and 10% methylene chloride
The Bond Elut NH2 tubes are separated, cholesterol is eluted from both, and finally the
di- and monoglycerides are eluted from the upper NH2 tube.
Elution 4:
(Cholesterol)
5% ethyl acetate in hexane
(Diglycerides)
Simpson, N & Van Horne,
C (eds) (1993) The 15% ethyl acetate in hexane
Handbook of Sorbent (Monoglycerides)
Extraction Technology.
Varian, Inc., Walnut Creek 2:1 chloroform:methanol
CA, USA.

Silica-Based SPE
Ion Exchange Silica SPE

Ion exchange phases are more dependent on pH, ionic strength, and counter-ion
strength than on solvent strength. These phases depend on ionic interactions as
the primary retention mechanism.
Bond Elut SAX

CH3 CI- • Retains compounds that elute from weak anion exchange sorbents
• Selectivity can be user-modified for increased flexibility
O Si (CH2)3 N+ (CH3)3 • Minimal non-polar interactions
CH3 Bond Elut SAX is a strong anion exchange sorbent ideally suited for the extraction of compounds
such as carboxylic acids, which may not retain effectively on weak anion exchange sorbents.

Aqueous, Anion exchange Weak acidic

Silica-Based SPE
Bond Elut SAX

40 μm 120 μm
LRC Cartridges
100 mg, 10 mL 50/pk 12113017 14113017
500 mg, 10 mL 50/pk 12113043 14113043
50 mg, 1 mL 100/pk 12102079 14102079
100 mg, 1 mL 100/pk 12102017 14102017
100 mg, 3 mL 50/pk 12102125
500 mg, 3 mL 50/pk 12102044 14102044
500 mg, 6 mL 30/pk 12102144
1 g, 3 mL 50/pk 12102087
1 g, 6 mL 30/pk 12256013 14256013
2 g, 6 mL 30/pk 12256051
2 g, 12 mL 20/pk 12256021 14256021
5 g, 20 mL 20/pk 12256029 14256029
10 g, 60 mL 16/pk 12256037 14256037
Bond Elut Jr
500 mg 100/pk 12162044B
1g 100/pk 12166013B
Other Formats
VersaPlate Formats
Description Particle Size (μm) 50 mg
Preassembled 96-well plate 40 75408050
VersaPlate tubes, 96/pk 40 75508050

Silica-Based SPE
Bond Elut SCX

CH3 • Useful for compounds with both cationic and non-polar characteristics
• Superior cleanup from a single sorbent
O Si (CH2)2 SO3-H+ • Very low pKa ligand elicits strong analyte interaction
CH3 Bond Elut SCX is a strong cation exchanger with a very low pKa. Although the pKa is similar
to Bond Elut PRS, the presence of the benzene ring in the functional group increases the
potential for non-polar interactions. This non-polar characteristic becomes particularly
important when conducting ion exchange from aqueous systems, where selectivity towards
compounds exhibiting cationic and non-polar character is seen.
References
Codony, R, Compañó, R, Granados, M,
Garcia-Regueiro, JA & Dolors Prat, M (2002) Aqueous, biological Cation exchange Weak basic
Residue analysis of macrolides in poultry muscle fluids, buffered compounds
by liquid chromatography-electrospray mass organics
spectrometry. J. Chromatogr. A, 959, 131-141.
Horie, M, Saito, K, Ishii, R, Yoshida, T, Haramaki,
Y & Nakazawa, H (1998) Simultaneous determination Bond Elut SCX
of five macrolide antibiotics in meat by high
40 μm 120 μm
performance liquid chromatography.
J. Chromatogr. A, 812, 295-302. Description Unit Particle Size Particle Size
LRC Cartridges
Stubbings, G, Tarbin, J, Cooper, A, Shaman, M.
Bigwood, T & Robb, P (2005) A multi-residue 100 mg, 10 mL 50/pk 12113013 14113013
cation-exchange clean up procedure for basic 500 mg, 10 mL 50/pk 12113039 14113039
drugs in produce of animal origin. Analyt. Chim.
Acta, 547, 262-268. Straight Barrel Cartridges
50 mg, 1 mL 100/pk 12102075 14102075
100 mg, 1 mL 100/pk 12102013 14102013
100 mg, 3 mL 50/pk 12102098
500 mg, 3 mL 50/pk 12102040 14102040
1 g, 6 mL 30/pk 12256011 14256011
1.5 g, 3 mL 50/pk 12102104
2 g, 12 mL 20/pk 12256053 14256019
3 g, 6 mL 30/pk 12256054
5 g, 20 mL 20/pk 14256027
10 g, 60 mL 16/pk 14256035
(Continued)

Silica-Based SPE
Bond Elut SCX

40 μm 120 μm
Bond Elut Jr
500 mg 100/pk 12162040B
1g 100/pk 12166011B
Other Formats
VersaPlate Formats
Bond Elut PRS

CH3 • Strong cation exchange sorbent, also capable of polar and hydrogen bonding interactions
• No appreciable non-polar interactions
O Si (CH2)3 SO3-Na+ • Unique selectivity properties
CH3 Bond Elut PRS is a strong cation exchange sorbent that is also relatively high in polarity.
With no appreciable degree of hydrophobicity in non-polar solvents, PRS is capable of polar
and hydrogen bonding interactions. Due to the very low pKa of PRS, it is recommended
for weaker cationic species such as pyridinium compounds.

Aqueous, biological Cation exchange Basic compounds
fluids, buffered (amine + pyridinium
organics containing)
Bond Elut PRS

LRC Cartridges
100 mg, 10 mL 50/pk 12113012
500 mg, 10 mL 50/pk 12113038
50 mg, 1 mL 100/pk 12102074
100 mg, 1 mL 100/pk 12102012
200 mg, 3 mL 50/pk 12102094
500 mg, 3 mL 50/pk 12102039
1 g, 6 mL 30/pk 12256010

Silica-Based SPE
Bond Elut PSA

CH3 • Alternative choice to Bond Elut NH2 for polar compounds
• Higher ionic capacity than NH2
O Si (CH2)3 NH NH2
Bond Elut PSA is an alkylated amine sorbent that contains two different amino functionalities –
CH3
one secondary and one primary. This gives a slightly higher pKa and ionic capacity compared to
Bond Elut NH2. PSA has a significantly higher carbon load than most amino functional sorbents,
thus is a better choice for polar compounds, which retain too strongly on Bond Elut NH2.

Aqueous, biological Strong anion exchange Acidic compounds
fluids, buffered (Fruit acid removal for
organics QuEChERS)
Bond Elut PSA

LRC Cartridges
100 mg, 10 mL 50/pk 12113015
500 mg, 10 mL 50/pk 12113041
50 mg, 1 mL 100/pk 12102077
100 mg, 1 mL 100/pk 12102015
500 mg, 3 mL 50/pk 12102042
1 g, 6 mL 30/pk 12256140
2 g, 12 mL 20/pk 12256055
Bond Elut Jr
500 mg 100/pk 12162042B
1g 100/pk 12166050B

Silica-Based SPE
Bond Elut CBA

CH3 • Cation exchange with no need for extreme basic conditions
O • Wider selectivity range provides more eluent options
O Si (CH2)2 • Polar or non-polar depending on matrix or solvent
CH3 OH
CBA is a mid-polarity sorbent and weak cation exchanger (pKa 4.8). It can be used with a wider
range of counter-ions than lower pKa sorbents like SCX, and will demonstrate easier elution of
quaternary amine functionalized analytes.

References Aqueous samples, Weak cation exchange Strong and weak bases
biological fluids
Murayama, N. & Sudo, K (1997) High performance
liquid chromatographic method for determination
of DX-9065a, a novel anticoagulant, in human
urine and feces using cation-exchange solid-phase Bond Elut CBA
extraction. J. Chromatogr. Biomed. Appl., 692,
389-396. Description Unit Part No.
LRC Cartridges
100 mg, 10 mL 50/pk 12113011
500 mg, 10 mL 50/pk 12113037
50 mg, 1 mL 100/pk 12102073
100 mg, 1 mL 100/pk 12102011
200 mg, 3 mL 50/pk 12102097
200 mg, 3 mL 50/pk 12102124
500 mg, 3 mL 50/pk 12102038
1 g, 6 mL 30/pk 12256009
2 g, 12 mL 20/pk 12256058

Silica-Based SPE
Bond Elut DEA

CH3 • Weak anion exchanger
CH3 • More polar than C8 but less polar than C2 or CN
O Si (CH2)3 N • Alkyl side chains confer moderately non-polar characteristics
CH3
CH3 Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties but with a slightly
lower capacity as an anion exchange sorbent. DEA has a moderately non-polar character due
to the alkyl side chains on the amino functionality. These groups still afford a medium level of
polarity, higher than C8 but less polar than C2 or CN-E.

References
Kline, W., Matuszewski, B & Bayne, W (1990) Water, biological fluids, Weak anion exchange Weak and strong
Determination of 4-amino-1-hydroxybutane-1, non-polar extracts acidic compounds
1-bisphosphonic acid in urine by automated
pre-column derivatization with 2,3-naphthalene
dicarboxyaldehyde and high performance liquid Bond Elut DEA
chromatography with fluorescence detection.
J. Chromatogr. Biomed.l Appl., 534, 139-149. 40 µm 120 µm
LRC Cartridges
100 mg, 10 mL 50/pk 12113016 14113016
500 mg, 10 mL 50/pk 12113042 14113042
50 mg, 1 mL 100/pk 12102078 14102078
100 mg, 1 mL 100/pk 12102016 14102016
500 mg, 3 mL 50/pk 12102043 14102043
VersaPlate Formats
Description Particle Size (µm) 50 mg 100 mg

Silica-Based SPE
Mixed Mode Silica SPE

Bond Elut AccuCAT
• SCX and SAX functionalities offer broad analyte extraction potential
• Ultra clean, mixed sorbent bed delivers reproducible extractions
• Compatible with many biological fluids for easy method transfer
Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation
exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT
is effective for the extraction of acidic, basic and neutral analytes from urine and other biological
samples. AccuCAT is particularly effective for catecholamine extraction from bio-fluids.
References
Andrzejewski, D, Roach, JAG, Gay, ML and Musser,
SM (2004) Analysis of coffee for the presence of Urine, plasma and Strong cation and anion exchange Catecholamines,
acrylamide by LC-MS/MS. J. Agric. Food Chem., 52, biological fluids, acrylamide in liquids
1996-2002. beverages and food and food
Lenders, JW, Eisenhofer, G, Armando, I, Keiser, HR,
Goldstein, DS and Kopin, IJ (1993) Determination of
metanephrines in plasma by liquid
chromatography with electrochemical detection.
Bond Elut AccuCAT
Clin. Chem., 39, 97-103. Description Unit Part No.
LRC Cartridges
200 mg, 10 mL 60/pk 12282005
600 mg, 10 mL 60/pk 12282001
200 mg, 3 mL 60/pk 12282003
200 mg, 6 mL 30/pk 12282004
400 mg, 6 mL 30/pk 12282006
600 mg, 3 mL 60/pk 12282002

Silica-Based SPE
Bond Elut Certify

Extraction of phencyclidine
(PCP) from human urine
• Special mixed-mode sorbent bed
using Bond Elut Certify
• Broad application range for aqueous extraction
• Bimodal, non-polar and strong cation exchange
The Bond Elut Certify extraction cartridge is a mixed mode sorbent containing non-polar
C8 strong cation exchanger functionalities. Certify is most commonly used to extract basic
(cationic) drugs from urine and blood, but it is also very effective for extraction of a wide
N range of compounds from a diverse range of aqueous matrices.
Phencyclidine
Urine, plasma, saliva, Non-polar and strong cation exchange Basic drugs, basic
blood, biological fluids drugs of abuse
Sorbent Conditioning: Bond Elut Certify

100% MeOH then 0.1 M phosphate 40 μm 120 μm
buffer, pH 6.0 Description Unit Particle Size Particle Size
Sample Treatment: LRC Cartridges
To 5 mL urine, add 2 mL 0.1 M 130 mg, 10 mL 50/pk 12113050 14113050
phosphate buffer, pH 6.0, and matrix 200 mg, 10 mL 50/pk 12113054 14113054
spike standard. Vortex, check that pH 300 mg, 10 mL 50/pk 12113052 14113052
lies between 5.0 and 7.0. Pass through
sorbent at <4 mL/min flow rate. Straight Barrel Cartridges
50 mg, 3 mL 50/pk 12105030
Interference Wash:
100 mg, 3 mL 50/pk 12102051 14102051
1. 1 mL 1.0 M AcOH then dry sorbent 100 mg, 6 mL 30/pk 12256146
under vacuum for 5 min
200 mg, 3 mL 50/pk 12102145
2. 6 mL MeOH, dry sorbent for 2 min
200 mg, 6 mL 30/pk 12256145
Analyte Elution: 300 mg, 3 mL 50/pk 12102081
2 mL 2% NH4OH in EtOAc 300 mg, 6 mL 30/pk 12102082
500 mg, 6 mL 30/pk 12102093 14102093
1 g, 6 mL 30/pk 12102085 14102085
Other Formats
VersaPlate Formats
Description Particle Size (μm) 25 mg 50 mg 100 mg
VersaPlate tubes, 96/pk 40 75509025 75509050 7550901C

Silica-Based SPE
Bond Elut Certify II

Extraction of THC-COOH
from human urine using
• Ideal for non-polar and anionic compounds
Bond Elut Certify II
• Optimized for acidic drug analysis
• Bimodal, non-polar and strong anion exchange
Bond Elut Certify II was developed specifically for the rapid and effective extraction of acidic
O OH drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode
THC-COOH
cartridge with non-polar C8 and strong anion exchange (SAX) functionalities. It has been
optimized for acidic drugs such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid,
OH salicylic acid, ibuprofen, acetaminophen and other compounds that possess both non-polar
and anionic characteristics.
O Typical Matrices Primary Extraction Mechanism Compound Types

Urine, plasma, saliva, Non-polar and strong anion exchange Acidic drugs,
blood, biological fluids acidic drugs of abuse
Sorbent Conditioning:
100% methanol then 0.1 M acetate
buffer, pH 7.0 Bond Elut Certify II
Sample Treatment: 40 μm 120 μm
To 6 mL urine, add 300 μL 10 M Description Unit Particle Size Particle Size
potassium hydroxide and matrix spike LRC Cartridges
standard. Vortex, hydrolyze at 60°C for 100 mg, 10 mL 50/pk 12113063
15 min, cool. Add 165 μL glacial acetic 200 mg, 10 mL 50/pk 12113051 14113051
acid and 2 mL 95% 0.1 M acetate
buffer/5% MeOH, pH 7.0. Adjust sample Straight Barrel Cartridges
pH to between 4.5 and 6.5 with glacial 50 mg, 3 mL 50/pk 12105031
acetic acid. Pass through sorbent at 200 mg, 3 mL 50/pk 12102080 14102080
< 4 mL/min flow rate.
500 mg, 6 mL 30/pk 12102084 14102084
Interference Wash: 1 g, 6 mL 30/pk 12102088 14102088
1. 10 mL 50:50 H20/MeOH, then dry Other Formats
sorbent under vacuum for 10 min Prospekt cartridge, 800 Series 96/pk 12281102
2. 2 mL EtOAc, dry sorbent for 0.5 min
Analyte Elution:
2 mL 1% AcOH in
25% EtOAc/75% hexane

Inorganic SPE
Inorganic SPE
The following SPE phases have varying degrees of polarity and surface acidity or basicity.
They are primarily used to retain polar analytes. For these phases, solvent retention generally
decreases as the solvent becomes more polar.
Bond Elut Florisil

• Pesticide Residue (PR) grade
• For cleanup of polar interferences from non-polar samples
• Economical
• Fast flow, ideal for viscous samples
Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar in nature and ideal for
the isolation of polar compounds from non-polar matrices. The larger particle size of the sorbent
enables fast flow for large sample volumes and is therefore an attractive alternative to silica
if the sample matrix is particularly viscous.

Non-polar organics Polar compounds Organic extracts,
non-polar
environmental extracts
Bond Elut Florisil

LRC Cartridges
500 mg, 10 mL 50/pk 12113049
100 mg, 1 mL 100/pk 12102024
500 mg, 3 mL 50/pk 12102050
1 g, 3 mL 50/pk 12102109
1 g, 6 mL 30/pk 12256014
1 g, 20 mL 20/pk 12256047
2 g, 12 mL 20/pk 12256022
2 g, 20 mL 20/pk 12256046
5 g, 20 mL 20/pk 12256030
10 g, 60 mL 16/pk 12256038
Bond Elut Jr
500 mg, 100/pk 100/pk 12162050B
1 g, 100/pk 100/pk 12166014B
Other Formats

Inorganic SPE
Bond Elut Alumina

• Available in acidic (A), basic (B) and neutral (N) formats
• High extraction efficiency
• Better high pH stability than unfunctionalized silica
Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly
more stable under high pH conditions than unfunctionalized silica. The small particle size
of the Bond Elut Alumina range ensures high extraction efficiency even when small bed
masses are used.

Non-polar organics Polar Polar cleanup
Bond Elut Alumina A

50 mg, 1 mL 100/pk 12102069
500 mg, 3 mL 50/pk 12102047
1 g, 6 mL 30/pk 12256043
Bond Elut Alumina B

500 mg, 3 mL 50/pk 12102048
1 g, 6 mL 30/pk 12256044
Bond Elut Jr
500 mg 100/pk 12162048B
Bond Elut Alumina N

LRC Cartridges
500 mg 50/pk 12113048
100 mg, 1 mL 100/pk 12102023
500 mg, 3 mL 50/pk 12102049
20 g, 60 mL 16/pk 12256059
Bond Elut Jr
500 mg 100/pk 12162049B
1g 100/pk 12166045B

Inorganic SPE
Bond Elut Sodium Sulfate Drying Cartridges

• Highly effective pre-packed dessicant
• Clean ACS grade, anhydrous sodium sulfate
• Pre-packed for convenience
Simplify sodium sulfate mediated drying steps by using cartridges pre-packed with ACS
grade, granular anhydrous sodium sulfate. Available in three formats (LRC, Bond Elut Jr
and straight barrels).
Bond Elut Jr cartridges have top and bottom luer fittings allowing easy sample processing when
used in conjunction with standard SPE cartridges. Bond Elut LRC cartridges have a large reservoir
above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.
Bond Elut Sodium Sulfate Drying Cartridges

LRC Cartridges
1 g, 10 mL 100/pk 12131033
15 g, 60 mL 100/pk 12132004
Bond Elut Jr
1.4 g 100/pk 12162052B
2.2 g 100/pk 12162054B
3g 100/pk 12162051B
Tips & Tools

Agilent offers Bond Elut Adapters compatible with these
tube formats.
Turn to page 212.

Specialty SPE
Specialty SPE
Bond Elut Carbon
• Excellent retention for small organics, including those that are too polar to retain
on C18 or polymeric SPE
• Removal of chlorophyll and other pigments leads to fewer chromatographic
or mass interferences
• Broader retention and easier elution of analytes across the polarity range, for improved
multi-residue analysis
Bond Elut Carbon cartridges are packed with ultra-pure graphitized carbon particles that have
been optimized for the absorption of pigments in food, fruits and vegetables, and small organic
residues in waste water. The powerful retention mechanisms of these products are appropriate
for a broad range of analytes. In addition, careful manufacturing techniques result in lower
carbon fines on the wall of the device.
References
Japanese Positive List System for Agricultural
Chemical Residues in Food. http://www.ffcr.or.jp Organic plant Wide range non-polar retention Cleanup of pigments
and tissue extracts and endogenous plant
EPA Method 535: Measurement of extracts for pesticide
Chloroacetanilide and Other Acetamide Herbicide
Degradates In Drinking Water By Solid Phase
and herbicide analysis
Extraction and Liquid Chromatography/Tandem
Mass Spectrometry (LC/MS/MS).

Specialty SPE
Bond Elut Carbon

50 mg, 1 mL 100/pk 126414
100 mg, 1 mL 100/pk 126418
250 mg, 6 mL 30/pk 12102201
500 mg, 6 mL 30/pk 12252201
Bond Elut Carbon/NH2

500/500 mg, 6 mL 30/pk 12252202
500/500 mg, 20 mL 20/pk 3664325032
Bond Elut Carbon/PSA

250/250 mg, 3 mL 50/pk 12102042C250
500/500 mg, 6 mL 30/pk 12102042C500

Specialty SPE
Method for the simultaneous monitoring of pesticide residues in agricultural products –

extraction, refining (cleanup) and quantitative analysis
For vegetables and foods For grains, cereals and beans
Sample size 10.0 g Sample size 20.0 g
Extraction Extraction
Bond Elut C18 (1 g)

1. Condition with 10 mL ACN
2. Apply 20 mL extract
3. Extract with 2 mL ACN
Remove water with Na2SO4
Filter, concentrate
Bond Elut Carbon/NH2 6 mL (500 mg/500 mg), P/N 12252202

1. Condition with 10 mL toluene:ACN (1:3)
2. Apply 20 mL extract
3. Extract with 10 mL toluene:ACN (1:3)
Concentrate
GC/MS(MS) analysis (DB-35ms UI)

Specialty SPE
Bond Elut Atrazine

• Large particle size allows flow of large sample volumes
• Controlled carbon content enhances atrazine selectivity
• Large bed mass offers optimized capacity for atrazine
Bond Elut Atrazine is a specially bonded, low load, high-flow C18 phase designed for atrazine
extraction. Methods are fast, reproducible and require minimal organic solvent consumption.

Water sources Non-polar Atrazine and atrazine
by-products
Bond Elut Atrazine

120 μm
Description Unit Particle Size
3 g, 20 mL 20/pk 12256111
Bond Elut Cellulose

• High purity micro-granular cellulose with high α-cellulose content
• Stable across a broad pH range
• Extremely low metal content (Fe, Cu <5 ppm)
Bond Elut Cellulose columns use a pure micro-granular cellulose powder that is packed between
two 20 μm polyethylene frits. The cellulose phase is very stable over a wide pH range with
extremely low metal content. The combination of surface area and polymeric structure results
in a sorbent with excellent capacity. The cellulose media contains numerous hydroxyl groups;
because of it polar nature, it is able to accept high loading of many polar substances from
aqueous and organic phases.

Aqueous and Polar (Hydroxyl) Polar
non-polar organics impurities/compounds
Bond Elut Cellulose

300 g, 3 mL 500/pk 12102095

Specialty SPE
Bond Elut PCB

• Optimized bed mass affords excellent extraction reproducibility
• Special dual-phase enhances PCB selectivity
• All extractions can be completed with one solvent to simplify procedures
Bond Elut PCB is a specially designed sorbent which allows for the easy extraction of
polychlorinated biphenyl (PCB) compounds from a variety of matrices. Desired analytes
can be loaded and eluted using a simple, single solvent method prior to analysis by GC/ECD.

Water sources Non-polar PCBs
Bond Elut PCB

1 g, 3 mL 50/pk 12105032
Tips & Tools

Agilent offers a variety of e-Seminars and on-site training
to help you learn how to be a more effective chromatographer.
For more information, visit www.agilent.com/chem/education

Specialty SPE
Bond Elut Mycotoxin

• Simple methodology saves time and increases throughput
• Use with a broad range of food matrices
• Economic and time-saving alternative to immunoaffinity techniques
Bond Elut Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene
and zearalenone analysis. Results are comparable or superior to competing methods, including
immunoaffinity columns (IAC) and charcoal/alumina columns. The sorbent is a proprietary silica-
based ion exchange material.
The Bond Elut Mycotoxin method for extraction and cleanup is successful with a variety of food
and grain sample types, including wheat, corn, durum, oats, bread, muesli and infant food.
Bond Elut Mycotoxin is easy to use and acts in a selective non-retention way – the toxin analytes
pass through the cartridge while the food matrix components are retained.
References
Kiötzel, M, Lauber, U & Humpf, H-U (2006)
A new solid phase extraction clean-up method for Aqueous and polar Ionic cleanup Mycotoxins
the determination of 12 type A and B trichothecenes organic grain extracts (trichothecenes and
in cereals and cereal-based food by LC-MS/MS. (beer, wine, sake) zearalenones)
Mol. Nutr. Food Res, 50, 261-269.
Bretz, M, Beyer, M, Cramer, B & Humpf, H-U
(2006) Stable isotope dilution analysis of Bond Elut Mycotoxin
the fusarium mycotoxins deoxynivalenol and
3-acetyldeoxynivalenol. Mol. Nutr. Food Res., Description Unit Part No.
50, 251-260. 500 mg, 3 mL 50/pk 12102167
Bond Elut Jr
500 mg 100/pk 12165001B

Specialty SPE
General Mycotoxin Methods
For Solids
Wheat beer
1. Finely grind 25 g sample and extract % Recovery % RSD % Recovery % RSD
with a solution of 100 mL Mycotoxin 35 ng/g 350 ng/g
acetonitrile/water (80:20) by blending
at high speed for 3 min. For simultaneous DON 92.0 2.6 95.5 1.5
determination of zearalenone, spike ZEA 116.0 6.1 101.9 1.3
extract at a level of 50 ng/g sample T-2 61.3 12.6 60.1 1.1
with zearalanone (ZAN) solution in HT-2 81.8 5.6 76.1 1.4
acetonitrile internal standard. Filter.
2. Pass 4 mL of the filtrate through
a Bond Elut Mycotoxin column.
3. Evaporate 2 mL of eluate to dryness at
50°C under a gentle stream of nitrogen. Sake wine
4. Reconstitute in 0.5 mL ACN/H2O
(1:4; v/v). Inject 10 μL into LC % Recovery % RSD % Recovery % RSD
for analysis. Mycotoxin 35 ng/g 350 ng/g
DON 94.3 7.4 96.8 0.5
ZEA 99.3 1.3 99.8 0.8
For Beverages
T-2 101.3 1.3 66.0 0.9
1. Sonicate the beverage sample HT-2 113.9 8.3 111.0 1.0
for 30 min. Filter.
2. Pass 4 mL of the filtrated sample extract
through a Bond Elut Mycotoxin cartridge.
3. Evaporate 2 mL of the eluate to
dryness at 50°C under a gentle
stream of nitrogen.
4. Reconstitute in 0.5 mL ACN/H2O
(20/80; v/v).
5. Inject into LC/MS/MS. This application shows the optimized extraction and cleanup of type A- and B-trichothecenes
[deoxynivalenol [DON], HT-2 toxin [HT-2], T-2 toxin [T-2] and zearalenone (ZEA).

Specialty SPE
Bond Elut PBA

CH3 H • Unique phenylboronic acid sorbent
O Si (CH2)3 N
• High specificity for cis-diol compounds
CH3 • Amenable for a broad range of bio-molecule applications
B(OH)2
Bond Elut PBA is a unique silica SPE sorbent containing a phenylboronic acid functionality
that can retain analytes via a reversible covalent bond. This very strong covalent retention
mechanism enables high specificity and cleanliness. The boronate group has a strong affinity
for cis-diol containing compounds such as catechols, nucleic acids, some proteins, carbohydrates
and PEG compounds. Aminoalcohols, alpha-hydroxy amides, keto compounds, and others can
also be retained.

Plasma, urine, aqueous Covalent bonding cis-diol-containing
and biological fluids compounds,
catecholamines,
ribonucleotides, amino
alcohols, diketo and
triketo compounds
Bond Elut PBA

LRC Cartridges
100 mg, 10 mL 50/pk 12113018
100 mg, 1 mL 20/pk 12102018
100 mg, 1 mL 100/pk 12102019
500 mg, 6 mL 30/pk 12102105
Generic Method
Condition: Compound Class Examples

1. 70:30 H20:ACN with 1% TFA Polyhydroxy Mannitol, fructose-6-phosphate, CDP-ethanol-amine,
2. 50 mM phosphate buffer (pH 10) glycoproteins
Aromatic O-dihydroxy Catechols, tannins, epinephrine
Sample Addition:
α-Hydroxy acids Lactate, 6-phospho-gluconate
Sample should be buffered to pH 8.5
with 50 mM phosphate buffer Aromatic O-hydroxy acids and amines Salicylate, salicylamide
1,3-Dihydroxy Tris, pyridoxine
Interference Wash:
10 mM phosphate buffer (pH 8.5) Diketo & triketo Dehydroascorbic acid, benzil, alloxan
with 5% ACN Other dihydroxys Steroids, prostaglandins
Analyte Elution:
70:30 H2O:ACN with 1% TFA

Specialty SPE
EnvirElut
• Extreme purity offers cleanliness in extract
• High capacity allows for the processing of large sample volumes
• Broad compound specificity
EnvirElut sorbents are specially designed for the extraction of a wide range of compounds from
aqueous matrices. EnvirElut Herbicides, PAH and Pesticides are available in standard SPE straight
barrel cartridges, which can be used on conventional Vac Elut vacuum manifolds.

Water sources, Non-polar Pesticide and industrial
extracted soil samples chemical residues
EnvirElut
500 mg, 3 mL (Herbicide) 50/pk 12272006
1 g, 6 mL (PAH) 30/pk 12272005
500 mg, 6 mL (Pesticide) 30/pk 12272004

Diatomaceous Earth Sorbents

Chem Elut and Hydromatrix
• High purity sorbent supported liquid extraction (SLE) applications
• Available in pre-packed cartridges or bulk
• Packing method delivers excellent tube-to-tube reproducibility
Chem Elut is an economical broad performance sorbent for rapid, general sample preparation
of biological samples such as plasma, serum, whole blood and urine. Chem Elut products are
available in buffered and unbuffered formats. The buffered devices can be used for simple
scrubbing operations on organic reactions. The base-treated cartridge can remove residual
acid compounds from a variety of matrices.
Hydromatrix is a high purity, inert diatomaceous earth sorbent available in 96-well plates
and as bulk material, offering end user flexibility and an excellent diversity of applications.
References
Plum, J & Daldrup, T (1986) Detection of digoxin,
digitoxin, their cardioactive metabolites Aqueous, biological Solid supported LLE Nitrosamines,
and derivatives by high performance liquid fluids, organic reaction pesticides, herbicides
chromatography and high performance mixtures (scavenging)
liquidchromatographyradioimmunoassay.
J. Chromatogr. A, 377, 221-231.
Biondi, PA, Guidotti, L, Montana, M, Manca, F,
Brambilla, G & Lucarelli, C (1991) A derivatization
procedure suitable for HPLC analysis of clenbuterol.
J. Chromatogr. Sci., 29(5), 190-193.
Raou, S, Gremaud, E, Biaudet, J & Turesky, R (1997)
Rapid solid-phase extraction method for the
detection of volatile nitrosamines in food.
J. Agricultural and Food Chem., 45, 4706-4713.
European method for azodyes in manufactures,
EN 1471.

Chem Elut Cartridges

Buffered pH Volume (mL) Unit Part No.
4.5 3 100/pk 12198004
9.0 3 100/pk 12198005
Unbuffered 0.3 100/pk 12198001
1 100/pk 12198002
3 100/pk 12198003
5 100/pk 12198006
10 100/pk 12198007
20 100/pk 12198008
50 50/pk 12198009
100 25/pk 12198010
300 15/pk 12198011
Hydromatrix
Description Part No.
Hydromatrix bulk material, 1 kg 198003
Hydromatrix bulk material, 4 kg 198004
Combilute plate, 200 mg, 65401507

Other Formats
Combilute plate, 200 mg 65401507
Preassembled 96-well plate 75430260
VersaPlate tubes, 96/pk 75530260

Bulk SPE
Bulk SPE
Bondesil Bulk Sorbents
• Ideal for dispersive cleanup techniques
• Advanced bonding offers reproducible batch-to-batch performance
• Multi-kilo quantities available upon request
Bondesil Bulk Sorbents

Particle Size
Description (μm) Unit Part No.
2OH (Diol) 40 100 g 12213030
Alumina-N 25 1000 g 12213073
C18 40 10 g 12213011
40 100 g 12213012
40 1000 g 12213013
120 100 g 14213012
120 1000 g 14213013
C18 OH 40 100 g 12213049
C2 40 100 g 12213006
C8 40 100 g 12213009
CBA 40 100 g 12213033
CN-E 40 100 g 12213061
DEA 40 100 g 12213047
ENV (polymeric) 125 100 g 12216061
EnvirElut 40 100 g 12214016
40 1000 g 12214019
FL 200 100 g 12214013
200 1000 g 12214015
NH2 40 10 g 12213020
40 100 g 12213021
120 100 g 14213021
PH 40 100 g 12213015
PRS 40 100 g 12213036
PSA 40 10 g 12213023
40 100 g 12213024
40 1000 g 12213025
SAX 40 100 g 12213042
40 1000 g 12213043
SCX 40 100 g 12213039
40 1000 g 12213040
120 100 g 14213039
SI 40 500 g 12213001

Bulk SPE
Mega Bond Elut Flash

• Convenient disposable cartridges eliminate the need for packing glass columns
• Flexible "open" tube design for either liquid or solid samples
• Reliable, consistent flow characteristics deliver high-resolution performance
Mega Bond Elut Flash cartridges offer excellent levels of performance and productivity
for the purification of organic compounds, but also for scale-up, solid phase extraction.
Pre-packed, disposable cartridges offer greater convenience than glass columns that
require washing, drying and re-packing after every sample.
Mega Bond Elut Flash

Sorbent Mass 40 μm
Description (g) Volume (mL) Unit Particle Size
C18 1 60 16/pk 12256060
2 12 20/pk 12256015
5 20 20/pk 12256023
10 60 16/pk 12256031
20 60 16/pk 12256078
25 150 8/pk 12256079
50 150 8/pk 12256080
75 150 8/pk 12256081
NH2 2 12 20/pk 12256020
5 20 16/pk 12256028
10 60 16/pk 12256036
20 60 16/pk 12256074
25 150 8/pk 12256075
50 150 8/pk 12256076
70 150 8/pk 12256077
SCX 20 60 16/pk 12256066
25 150 8/pk 12256070
50 150 8/pk 12256072
70 150 8/pk 12256073
SI 2 12 20/pk 12256018
5 20 20/pk 12256026
10 60 16/pk 12256034
15 60 16/pk 12256068
20 150 16/pk 12256042
25 150 8/pk 12256069
50 150 8/pk 12256067
70 150 8/pk 12256071

Bond Elut Accessories

• Available with many of our most popular Bond Elut sorbents
• Fast revalidation of cartridge to 96-well SPE methods
• Low-profile, automation-friendly design
Bond Elut 96 Round-well plates Conversion of cartridge-based methods to an automation-friendly 96-well format has never been
easier or faster. The same trusted silica-based sorbents in Bond Elut cartridge products are now
available in the streamlined Bond Elut 96-well plates. Bond Elut 96 components are specially
formulated to offer superior cleanliness, flow reproducibility, and reliability.
Polymeric Sorbents
Description Loading (mg) Part No.
Plexa 10 A4969010
30 A4969030
Plexa PCX 10 A4968010
30 A4968030
Plexa PAX 10 A4967010
30 A4967030
LMS 10 A4961010
NEXUS 30 A4962030
All Bond Elut silica 96 round-well plates are 40 μm particle size

Silica Sorbents
C2 50 A4961150
100 A496111C
C8 25 A4960325
50 A4960350
100 A496031C
C18 25 A4960125
50 A4960150
100 A496011C
C18 OH 100 A496291C
CBA 25 A4960625
50 A4960650
100 A496061C
CH 25 A4962225
50 A4962250
100 A496221C
CN-E 25 A4960425
50 A4960450
100 A496041C
CN-U 50 A4961450
Certify 25 A4960925
50 A4960950
100 A496091C
PBA 100 A496121C
PH 100 A496151C
NH2 25 A4960525
50 A4960550
100 A496051C
SAX 100 A496301C
SCX 25 A4960725
50 A4960750
100 A496071C
All Bond Elut silica 96 round-well plates are 40 μm particle size


• Enhanced quality for trouble-free high throughput
• Added flexibility for method development
• Automation-friendly to free up operator time
Bond Elut 96 Square-well Plates are specially designed to offer superior cleanliness,
flow reproducibility and reliability, ensuring trouble-free, high throughput operation. Conversion
Bond Elut 96 Square-well plate of cartridge-based methods to an automation-friendly 96-well plate format has never been easier
or faster. The 2 mL wells accommodate the larger processing volumes from older methods,
making method transfer and revalidation quick and easy. The large 2 mL well volume adds
flexibility when developing new methods, for example, when larger wash volumes or higher
sorbent capacities are required. In addition, Bond Elut 96 Square-well is designed to ensure
compatibility with existing robots and vacuum manifolds.
Polymeric Sorbents
Plexa 10 A3969010
30 A3969030
Plexa PCX 10 A3968010
30 A3968030
Plexa PAX 10 A3967010
30 A3967030
LMS 10 A3961010
25 A3961025
NEXUS 60 A3962060

Silica Sorbents
Description 25 mg 50 mg 100 mg
C8 A3960325 A3960350 A396031C
C18 A3960125 A3960150 A396011C
C18-OH A3962925 A3962950 A396291C
CBA A3960625 A3960650 A396061C
Certify A3960925 A3960950 A396091C
NH2 A3960525 A3960550 A396051C
PH A3961525 A3961550 A396151C
PBA A396121C
SAX A3960825 A3960850 A396081C
SCX A3960725 A3960750 A396071C
Manifolds and Accessories

96-well manifold, acrylic 5133000
96-well manifold, shimset 12236104
Square-well collection plates, 2 mL 5133009
Square-well collection plates, 1 mL 5133008
Square-well collection plates, 350 μL 5133007
Square-well collection plate cover WA77040004
Sealing tape pad 12143105

Bond Elut Empty SPE Cartridges

• Made with high purity polypropylene for cleaner extracts
• Uniform batch-to-batch size for consistent performance
• Economical for everyday use
A variety of empty reservoirs is available for packing custom SPE cartridges with bulk Bondesil
or other desired sorbents. Cartridges are available from 1 to 60 mL. Order frits separately,
or see below for reservoirs with pre-installed frits.
Bond Elut Empty SPE Cartridges

Volume (mL) Unit Part No.
1 100/pk 12131007
3 100/pk 12131008
6 100/pk 12131009
12 100/pk 12131010
20 100/pk 12131011
60 100/pk 12131012
Bond Elut Empty SPE Cartridges with Two Frits

• Pre-installed frits for ease-of-use
• Broad range of filtration operations for maximum flexibility
• Customizable packing for specific applications
These clean polypropylene reservoirs contain two polypropylene frits pre-inserted, an ideal
configuration for simple filtration. For custom sorbent packing, additional frits can be purchased
separately. Available from 1 to 60 mL.
Bond Elut Empty SPE Cartridges with Two Frits

Volume (mL) Unit Part No.
1 100/pk 12131013
3 100/pk 12131014
6 100/pk 12131015
12 100/pk 12131016
20 100/pk 12131017
60 100/pk 12131018

20 μm Polypropylene Frits for SPE Cartridges

• Made with high-grade, clean polyethylene for clean extracts
• Pre-cut to correct size for accuracy
• Use with reservoirs or custom packing
These frits are pre-cut to fit into Bond Elut reservoirs for use in filtration applications or for custom
SPE sorbent packing.
20 μm Polypropylene Frits for SPE Cartridges

Diameter (mm) To Fit Tube Size (mL) Unit Part No.
6.4 1 100/pk 12131019
9.5 3 100/pk 12131020
12.7 6 100/pk 12131021
15.9 12 100/pk 12131022
Polypropylene Frits, 12131021 20.6 20 100/pk 12131023
27.0 60 100/pk 12131024

Bond Elut Adapters

• Connect SPE cartridges in series for large samples
• Expand cartridge volume for even more applications
• Transfer large-volume samples to any SPE cartridge
Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that
accommodates the tip of another cartridge, allowing the following configurations:
Bond Elut Adapters

Adapter cap for 1, 3 and 6 mL Bond Elut cartridges 15/pk 12131001
Adapter cap for 12 and 20 mL Bond Elut cartridges 10/pk 12131003
Adapter cap for 60 mL Bond Elut cartridges 10/pk 12131004
Bond Elut Adapter Configurations
Configuration 1: Stack two cartridges to perform multi-sorbent methods

Configuration 2 + 3: Increase any cartridge's volume by stacking an empty reservoir on top
of the device.
Configuration 4: Standard Luer-tipped syringes will fit into any Bond Elut adapter.
Gentle pressure can then be used to apply conditioning solvents, samples, rinsing solvents
and eluents. This configuration is particularly useful for single sample processing, where
a vacuum manifold is not required.
Configuration 5: For excessively large sample volumes, 1/8 in. OD tubing can be connected
to the end of an adapter and the sample can be drawn directly from the sample container via
1 2 3 4 5 high vacuum.

Luer Stopcocks
• Control flow rates during SPE
• Improve method reproducibility
• Instant isolation from vacuum reduces accidental tube drying
Luer stopcocks are used to provide independent flow control of each individual Bond Elut
cartridge when used with vacuum manifolds. They are made from solvent resistant high-grade
polypropylene, are reusable and can be readily cleaned using organic solvents such as
methanol or acetone.
Luer Stopcocks
Luer stopcocks, 12131005 Luer stopcocks 15/pk 12131005
ASPEC Adapter Caps

• Enhance the high-throughput compatibility of Bond Elut cartridges
• Converts 1, 3 and 6 mL cartridges for use in Gilson SPE systems
• Specially engineered for leak-free operation
Gilson-engineered caps produce a positive pressure seal with the needle in Gilson ASPEC, ASPEC
XL and ASPEC XL4 solid phase extraction systems.
ASPEC Adapter Caps

Gilson adapter cap, 1 mL, yellow 1000/pk 12131034
Gilson adapter cap, 12131034 Gildson adapter cap, 3 mL, blue 1000/pk 12131035

Vac Elut 20 Manifold

• Increased productivity/sample throughput
• Disposable needles eliminate cross contamination
• Rugged, reliable construction
Engineered to increase laboratory productivity, the corrosion-resistant Vac Elut 20 permits
simultaneous processing of up to 20 Bond Elut cartridges. The manifold’s clear glass base allows
careful monitoring of the entire sample collection process. Its compact, linear design requires
very little bench space.
The Vac Elut 20 vacuum control valve, vacuum gauge, and quick release valve are
mounted on the lid, away from the corrosive solvent stream and within convenient reach.
The solvent-resistant polypropylene rack is available in a variety of sizes to accommodate
collection tubes commonly used in sample preparation.
To minimize the risk of sample carryover, low-cost, disposable, medical grade polypropylene
delivery needles can be easily replaced. Polypropylene extender tips are also available as
a replacement for the standard needle valves, ensuring a direct path into the collection tube.
Correct sample identification is also ensured by an interlocking fit between the lid and internal
test tube rack.

Vac Elut 20 manifold with collection rack for 10 x 75 mm test tubes 12234105
Racks for Glass Basins
Standard glass basin 12234505
Collection rack for 10 x 75 mm test tubes 12234517
Replacement Components and Accessories
Polypropylene delivery needles, 25/pk 12234511
Replacement exit valve for glass basin 12234506
Replacement lid gasket 12234502
Vac Elut 20 lid cover 12234501
Vacuum gauge assembly 12234504

Vac Elut 20 Manifold Tall Glass Basin

• For extractions greater than 10 mL
• Transparent glass base allows you to monitor the whole collection operation
• Simple vacuum adjustment
The Vac Elut 20 with a large glass basin and collection rack accommodates larger 16 x 150 mm
test tubes. The same high quality material and features on the standard Vac Elut system are
Vac Elut 20 manifold tall glass basin, 12234104
incorporated on this special unit. These collection vessels can be utilized in combinatorial
chemistry applications using large boiling tubes for collection of purified synthesis mixtures,
or for any SPE extraction in which an elution volume greater than 10 mL is required.
Vac Elut 20 Manifold Tall Glass Basin

Vac Elut 20 Manifold with tall glass basin and collection rack for 16 x 150 mm test 12234104
tubes, complete system


Agilent manifolds and accessories complement the quality of our sorbents. Configurations
and individual components can be purchased, providing flexibility and increased capability
at any stage, from method development to high-throughput operation.

12-port vacuum manifold processing station 5982-9110
Includes rack for 16 x 100 mm tubes
Vac Elut 12 manifold, 5982-9110
Replacement Parts for Vacuum Manifolds
Manifold ball ring/vacuum quick release 5982-9106
Manifold exit valve replacement kit 5982-9107
Manifold vacuum gauge assembly with valve 5982-9108
White cover for 12-port manifold 5982-9111
Sealing gasket for 12-port manifold 5982-9112
Glass chamber for 12-port manifold 5982-9113
12-port rack for 13 x 75 mm tubes 5982-9114
Parts and Disposables for Cartridge Manifolds

Manifold disposable needle tip 20/pk 5982-9100
Manifold stainless steel needle with polypropylene coating 20/pk 5982-9101
Manifold short valve stopcock 20/pk 5982-9102
Manifold long valve stopcock 20/pk 5982-9103
Manifold male luer plugs 25/pk 5982-9104
Manifold needle tip ejector tool 5982-9105
Cartridge stacking adapters 12/pk 5982-9109

Vac Elut SPS 24 Manifold

• Closed operation prevents cross contamination
• Stainless steel tips deliver maximum extract purity
• Range of rack sizes covers most tube configurations
The Vac Elut SPS 24 allows simultaneous processing of up to 24 SPE cartridges. Like all Vac Elut
manifolds, the SPS 24 is made from durable, solvent-resistant materials and engineered to last.
The glass sides allow easy viewing of the entire sample collection process.
The ultimate feature of the SPS 24 manifold is its waste diversion funnel, which enables
all steps of the SPE procedure to be completed without removing the lid. Since the collection
rack is placed inside the unit before extraction begins, splash back and cross contamination
are eliminated, while hazardous waste and biohazard exposure are minimized.
Vac Elut SPS 24 manifold
Complete with replacement stainless steel delivery tips for maximum extract purity, the Vac Elut
SPS 24 system also includes a vacuum controller/release, collection rack, and port sealing plugs.
Racks for several different collection tube configurations are available.
Vac Elut SPS 24 Manifold

Vac Elut SPS 24 manifold with collection rack for 10 x 75 mm test tubes 12234003
Replacement Components and Accessories
Collection rack and funnel set for 12 or 15 mL conical tubes 12234027
Collection rack and funnel set for 12 x 75 mm test tubes 12234030
Elastic lid fasteners, 6/pk 12234034
SPS 24 lid cover 12234025
SPS 24 waste tower repair kit 12234005
Includes base exit tube, hose connector, washer, center tube, 900 connector elbow
Stainless steel delivery needles, 25/pk 12234038
Waste funnel for 12 x 75 or 13 x 100 mm test tubes, 5/pk 12234032

Vacuum Manifolds for 96-well Plates

• Can handle 96-well fixed position plates or second version to handle 96-well
flexible format plate
• Constructed with polypropylene base and polyethylene lid
• Small footprint
• Supplied with on/off valve, vacuum gauge, and fine vacuum control valve
• disposable reservoir tray collects excess sample and wash solvents
• Spacer inserts can be placed into the base so that collection plates of differing
heights can be processed (both deep-well and standard microtiter plates), ensuring
maximum penetration of the SPE plate into the collection plate and reducing
well-to-well contamination
• Solvent resistance gasket in the manifold lid
Vacuum Manifolds for 96-well Plates

96-well vacuum manifold base assembly 5185-5797
Includes base, vacuum gauge and needle valve
96-well vacuum manifold base assembly,
5185-5797

Parts and Disposables for 96-well Plate Manifolds

Base O-ring for 96-well plate manifold 5185-5779
Collection plate spacer for Agilent 1 mL deep-well, 12 mm 5185-5775
Collection plate spacer for microtiter plate and Agilent 0.5 mL 5185-5781
shallow well plate, 29 mm
Collection plate spacer for most industry-standard deep-well plates, 2 mm 5185-5780
Disposable reservoir tray for 96-well manifold 25/pk 5185-5782
Base O-ring, 5185-5779 96-well vacuum manifold base assembly 5185-5797
Includes base, vacuum gauge and needle valve
Lid for 96-fixed well vacuum manifold 5185-5798
Lid gasket for 96-well plate manifold 5185-5778
Luer adapters for 96-well flexible cartridge 25/pk 5185-5789
Needle valve for 96-well manifold 5185-5783
On/off valve for 96-well manifold 5185-5785
Vacuum gauge for 96-well manifold 5185-5786
Vacuum outlet (Ni plated) for 96-well manifold 5185-5784
Collection plate spacer in sizes to match

the collection plate used
Sealing Mats
Sealing mats help prevent sample contamination or evaporation that can occur when plates
are exposed to environmental conditions.
Sealing Mats
96-well plate sealing mats, round 50/pk 5042-1389
96-well plate sealing mats, square 10/pk 5982-9996

Disk SPE
Disk SPE
SPEC Disk SPE
• No loose sorbent means no channeling of sample
• Uniform flow and extraction properties offer robust performance
• Low elution volume affords excellent concentration of analyte, improving sensitivity
Using an advanced disk design, SPEC delivers superior flow characteristics and trouble-free
automation. Due to the low volume of the extraction bed, very low elution volumes can be
used. This means that, in some applications, evaporation and reconstitution steps can be
eliminated, resulting in accelerated sample processing times. The combination of low bed
masses, ultra-clean base materials and a broad toolbox of selectivities delivers higher
recoveries free of the matrix interferences that can cause ion suppression.
SPEC provides high recoveries at low elution volumes – as low as 100 μL. This is due to the
very high surface area yet small physical volume of the monolithic disk. Overall, extraction
efficiency is very high for this format of sample preparation product, and the range of
functionalities allows fast method development.

Disk SPE
Unique phases available in SPEC 96-well and SPE tube formats
Uniform recovery and reproducibility between wells from the same well plate
• DAU – This functionalized SPEC disk is specifically

designed for the analysis of drugs of abuse in urine.
Its unique sorbent chemistry results in excellent
sample cleanup and concentration of samples
prior to GC/MS and LC/MS.
• MP1 – SPEC MP1 is a mixed-mode, non-polar/SCX

monolithic disk ideal for analytes with polar functional %
groups in plasma. The dual retention mechanism 100
results in cleaner extracts. The SCX functionality
strongly binds polar basic analytes allowing rigorous H
G
washing steps to be employed. Bond Elut Certify F
E
offers similar selectivity to SPEC MP1. D
C
0 B
• MP3 – SPEC MP3 is slightly more polar than MP1, 1 2 3 4 5 6 7 8 9 10 11 12 A
making it ideal for hydrophobic analytes that would
bind too strongly to MP1. MP3 chemistry is
particularly suited to the extraction of opiate alkaloids
from biological fluids.
Note the high recovery (y axis) with an average deviation across the 96 wells of just 3.2% (well positions are shown on the x and z axes). SPEC provides the predictable
flow characteristics analysts require for true walk-away automated processing. With SPEC you need not worry about clogging, and as an added benefit, the typically low
vacuum pressure requirement prevents cross-talk (e.g. spraying of fast running eluates between wells in the collection plate).

Disk SPE
SPEC 96-well Plates

When used on an automated platform, SPEC 96-well plates offer outstanding flow characteristics.
Flow across all 96-well plates is uniform and highly reproducible, meaning your recoveries are too.
SPEC 96-well Plates, 15 mg

Sorbent Phase Part No.
Silica-based Sorbents
C18 A59603
SPEC 96-well plate C18AR A59619
C18AR, 30 mg A5960330
C2 A59601
C8 A59602
CN A59606
DAU A596DAU
NH2 A59607
Phenyl A59610
Ion Exchange Sorbents
SAX A59605
SCX A59604
Mixed Mode Sorbents
MP1 A59611
MP3 A59620
Method Development Kit
C2, C8, C18, C18AR, CN, MP1, MP3, PH A59630

Disk SPE
SPEC SPE Cartridges

SPEC functionalities are also available in standard straight barrel tube format, offering flexibility
in sample size. Use on any standard vacuum manifold such as the Vac Elut 20 or SPS 24.
SPEC SPE Cartridges, 100/pk

Sorbent Phase Description Part No.
C18 15 mg, 3 mL A5320320
30 mg, 3 mL A5320330
C18AR 15 mg, 3 mL A5321920
30 mg, 3 mL A5321930
35 mg, 10 mL A5021935
C18/MP3 70 mg, 10 mL A5022570
C2 30 mg, 3 mL A5320130
C8 15 mg, 3 mL A5320220
30 mg, 3 mL A5320230
DAS 15 mg, 3 mL A532DAS
DAU 15 mg, 3 mL A532DAU
MP1 15 mg, 3 mL A5321120
30 mg, 3 mL A5321130
35 mg, 10 mL A5021135
70 mg, 10 mL A5021170
MP3 15 mg, 3 mL A5322020
30 mg, 3 mL A5322030
35 mg, 10 mL A5020735
NH2 15 mg, 3 mL A5320720
70 mg, 10 mL A5020770
Phenyl 15 mg, 3 mL A5321020
30 mg, 3 mL A5321030
SAX 15 mg, 3 mL A5320520
30 mg, 3 mL A5320530
35 mg, 10 mL A5020535
SPEC Disks and Accessories

SPEC disks, C8, 47 mm, 24/pk A74702
SPEC disks, C18AR, 47 mm, 20/pk A74819
SPEC disks, C18AR, 90 mm, 12/pk A79019
SPEC disks, C8, A74702, SPEC environmental disk manifold A712
and SPEC SPE Cartridges, C18, A5320320
SPEC environmental disk holder, 47 mm A713
SPEC flask, 1 L, 40/35 mm A714

Disk SPE
Empore Disk SPE

• Good flow of large sample volumes
• Range of versatile sorbent chemistries
• Available in two disk diameters for better performance
Empore extraction disks provide a high flow rate solution for large volume sample preparation,
and are available in a variety of bonded phases and two diameters, 47 and 90 mm. Increasing
the diameter of the disk gives better solvent flow rates through the disk.
Empore Disk SPE

Anion extraction disks, 47 mm 20/pk 12145012
Chelating extraction disks, 47 mm 20/pk 12145029
SDB-XC extraction disks, 47 mm 20/pk 12145010
SDB-XC extraction disks, 90 mm 10/pk 12145011
C8 extraction disks, 47 mm 20/pk 12145002

Micro-volume SPE
Micro-volume SPE
OMIX Tips
• Fast, uniform flow maximizes productivity and reproducibility
• Minimal peptide losses lead to higher recoveries
• Available in three phases and sizes to deliver better sequence coverage
OMIX tips with monolithic sorbent tip technology offer dependable purification and superior
results in proteomics research. Agilent OMIX pipette tips reliably purify and enrich femtomole
and picomole levels of peptides and proteins prior to MALDI-TOF or LC/MS/MS. The unique
monolithic sorbent technology used in OMIX consistently outperforms other tips by delivering
uniform flow and strong analyte-to-surface interactions. The high binding capacity of OMIX
delivers high productivity – the 10 μL tips bind up to 8 μg of peptide – twice as much as
tips from other suppliers. OMIX’s superior flow and exceptional binding capacity ensure
reliable recovery of your peptides, minimizing peptide loss during multi-aliquot, multi-tip
and evaporation steps.
OMIX Tips
C4 C18 SCX
Description Elution Volume Unit Part No. Part No. Part No.
10 μL 0.5 - 2 μL 1 x 96 tips A57009MB A57003MB A57004MB
Mini-Bed 6 x 96 tips A57009MBK A57003MBK
10 μL 2 - 10 μL 1 x 96 tips A5700910 A5700310 A5700410
6 x 96 tips A5700910K A5700310K
100 μL 10 - 100μL 1 x 96 tips A57009100 A57003100 A57004100
6 x 96 tips A57009100K A57003100K

Micro-volume SPE
OMIX Tips for Robotic Automation

• Fast, uniform flow maximizes productivity and reproducibility
• Small monolithic tip delivers low elution volumes, increasing sensitivity
and reducing solvent usage
• Vacuum-free processing improves reproducibility and shortens processing times
OMIX 96-well VersaPlate

OMIX automation-friendly 96-well monolithic SPE plates are specially designed to process
small samples. They offer small extraction beds with almost no dead volume. Elution is achieved
with microliter solvent volumes, allowing direct injection and improving assay speed and sample
throughput. OMIX tips are highly amenable to ADME/DMPK bioanalysis applications.
OMIX 96-well VersaPlate

OMIX 96-well VersaPlate, C4 with tubes A57109
OMIX C4 tubes only A57109A
OMIX 96-well VersaPlate, C18 with tubes A57103
OMIX C18 tubes only A57103A
OMIX 96-well VersaPlate, MP1 with tubes A57111
OMIX MP1 tubes only A57111A
OMIX Tips for Tomtec Quadra

Tomtec-compatible tips contain a slice of monolithic SPE material, allowing for vacuum-free
processing and walk-away automation. With hands-free SPE, the process becomes much more
streamlined and reproducible.
OMIX Tips for Tomtec Quadra

OMIX C18 for Tomtec Quadra, A57303SPL OMIX C18 1 x 96 tips A57303SPL
OMIX C18 5 rack x 96 tips A57303
OMIX MP1 1 x 96 tips A57311SPL
OMIX MP1 5 x 96 tips A57311
Close-up of OMIX tips for Tomtec Quadra

Micro-volume SPE
OMIX Tips for Hamilton Microlab STAR Line

Offering excellent versatility and end-user productivity enhancements, these tips have
an operating volume of 300 μL, allowing flexibility in sample size. Processing 96 samples
can be reduced to just a few minutes in certain applications.
OMIX Tips for Hamilton Microlab STAR Line, 300 μL

OMIX C18 for Hamilton 300 μL, A57403SPL
OMIX C18 1 x 96 tips A57403SPL
OMIX C18 5 x 96 tips A57403
OMIX MP1 1 x 96 tips A57411SPL
OMIX MP1 5 x 96 tips A57411
Close-up of OMIX tips for Hamilton
OMIX Tips for Hamilton STAR, MP1, 5 mg
Sample Pretreatment Conditioning

Add 200 μL 2% H3PO4 to 100 μL of human plasma Pre-mix 300 μL sample 3 times
Aspirate 300 μL and dispense into waste tray
Conditioning Washing
Aspirate 300 μL of methanol, dispense into waste tray Aspirate 300 μL of deionized water, dispense into waste tray
Aspirate 300 μL of water, dispense into waste tray Aspirate 300 μL of methanol, dispense into waste tray
Aspirate and dispense parameters

Washing Flow rate: 50 μL/s
Add 5 mL 0.1M HCI, 2 mL methanol Setting time: 3s
Vacuum extract for 1 min
Total extraction time: < 5 min
Albuterol Relative Recoveries

Amount (ng/mL) % Recovery
48.0 96
46.0 92
49.7 99
46.6 93
49.1 98
47.4 95
Mean recovery 96%, RSD 3%

QuEChERS
QUECHERS
Agilent's QuEChERS Kits provide an easy way to take advantage of the simple, time-saving
QuEChERS method – because they are pre-packaged to give you greater efficiency and reliability.
QuEChERS Kits help you take advantage of the benefits of the QuEChERS method through:
No guesswork or measuring – Pre-packed extraction and dispersive kits are assembled to
suit specific food types and screening protocols
Uniquely packaged extraction kits – Now available with or without 50 mL centrifuge tubes,
extraction kits package pre-weighed salts in anhydrous packets, enabling you to add the salts at
the appropriate time
Wide selection of dispersive kits – Sorbents and salts for dispersive SPE are supplied in 2 mL
or 15 mL centrifuge tubes, for 1 mL, 6 mL or 8 mL aliquot volumes, as specified by the various
QuEChERS methodologies
Ceramic homogenizers save 70% of your time per sample – Agilent's ceramic
homogenizers can cut your required extraction time from 60 seconds to less than 20 seconds

QuEChERS
Agilent Recommended Standard Operating Procedure for QuEChERS

In just 3 easy steps, you can prepare any fruit or vegetable sample for multi-class, multi-residue pesticide analysis.
Comminuted Sample: 10 g or 15 g
ADD ACETONITRILE
SELECT EXTRACTION KIT Selection criteria

• QuEChERS method
Buffered
• Compounds for screening
Buffered
Original Method Original Method AOAC 2007.1 Use buffered kits if base-sensitive
EN 15662 Method
10 g samples 15 g samples Method pesticides are present. Agilent
10 g samples
15 g samples recommends using buffered kits
as a first choice.
CHECK pH AND ADJUST TO 5 - 5.5

ADD INTERNAL STANDARD
SHAKE AND CENTRIFUGE
Aliquot: 1 mL, 6 mL or 8 mL*
SELECT DISPERSIVE SPE KIT Selection criteria

• QuEChERS method
• Food type to be analyzed
General Fruits & Fatty/Waxy Fruits General Fruits & Fatty/Waxy Fruits • Aliquot volume
Vegetables & Vegetables Vegetables & Vegetables
2 mL and 15 mL kits 2 mL and 15 mL kits 2 mL and 15 mL kits 2 mL and 15 mL kits
High Pigment Fruits &

Pigmented Fruits Pigmented Fruits Vegetables with
Fruits &
& Vegetables & Vegetables Fats, Pigments
Vegetables
2 mL and 15 mL kits 2 mL and 15 mL kits
2 mL and 15 mL kits 2 mL and 15 mL kits
AOAC Method EN Method
SHAKE AND CENTRIFUGE
Analysis
*Aliquot size is specified by the method, and kits are created for these specific amounts. For pesticides with acidic groups (phenoxyalcanoic acids),
analyze directly by LC/MS/MS at this point (skip the dispersive SPE stage). These acidic groups interact with the PSA that is part of the dispersive SPE step.
QUECHERS 229
QuEChERS
QuEChERS Extraction Kits

• Available with or without 50 mL centrifuge tubes and caps
• Include MgSO4, NaCl, or other salts for buffering; pre-weighed in anhydrous packet
Step 1: Extraction
Adding solvent and salts to a small (10 g or 15 g) comminuted fruit or vegetable sample enables you to extract the pesticides of interest into
the organic layer. However, adding a food sample with a high percentage of water directly to the salts may create an exothermic reaction that
can affect your analyte recoveries. Choose the extraction salt packet based on your method of analysis, AOAC or EN. The buffered extraction
salts are amenable for more liable pesticides.
Agilent pre-packages its QuEChERS salts and buffers in anhydrous packages. This allows you to add them after adding your solvent to the sample,
as specified in QuEChERS methodologies.
QuEChERS Extraction Kits

Packets Only
Ceramic
Method Buffered Contents Homogenizers With Tubes 50/pk 200/pk
AOAC Yes 6 g MgSO4; 1.5 g NaAcetate Yes 5982-5755CH 5982-6755CH 5982-7755CH
No 5982-5755 5982-6755 5982-7755
Original No 4 g MgSO4; 1 g NaCL Yes 5982-5550CH 5982-6650CH 5982-7550CH
(10 g samples)
No 5982-5550 5982-6650 5982-7550
Original No 6 g MgSO4; 1.5 g NaCL Yes 5982-5555CH 5982-6555CH 5982-7555CH
(15 g samples)
No 5982-5555 5982-6555 5982-7555
EN Yes 4 g MgSO4; 1 g NaCl; 1 g NaCitrate; Yes 5982-5650CH 5982-6650CH 5982-7650CH
0.5 g disodium citrate sesquihydrate
No 5982-5650 5982-6650 5982-7650
Acrylamides* No 4 g MgSO4; 0.5 g NaCl No 5982-5850
*Katerina Mastovaka and Steven J. Lehotay have done work to extend the scope of QuEChERS beyond fruits and vegetables(1), using it to extract acrylamides in potato chips and other fried foods.
1: "Rapid Sample Preparation Method for LC-MS/MS or GC-MS Analysis of Acrylamides in Various Food Matrices", J. Agric. Food Chem, 2006, 54, 7001-7008.

QuEChERS
QuEChERS Dispersive Kits

Step 2: Dispersive SPE Cleanup
Select the Dispersive SPE kit suited to the type of food being analyzed and the method you
are following. In this step, an aliquot of the sample extract from Step One is added to a 2 mL
or 15 mL centrifuge tube containing a small amount of SPE sorbent and MgSO4. The sorbent
will pull out interfering matrix materials from the sample, while the MgSO4 helps remove excess
water and improve analyte partitioning. Select kits are now available with ceramic homogenizers
(2 per tube). Their part numbers are designated by a CH.

AOAC 2007.01 European Method
Method EN 15662
Kit Contents Kit Contents
Kit Size Unit Part No. Part No.
General fruits 2 mL 100/pk 50 mg PSA 25 mg PSA
and vegetables: 150 mg MgSO4 150 mg MgSO4
Removes polar organic 5982-5022 5982-5021
acids, some sugars 5982-5022CH 5982-5021CH
and lipids
15 mL 50/pk 400 mg PSA 150 mg PSA
1200 mg MgSO4 900 mg MgSO4
5982-5058 5982-5056
5982-5058CH 5982-5056CH
Fruits and vegetables 2 mL 100/pk 50 mg PSA 25 mg PSA
with fats and waxes: 50 mg C18EC 25 mg C18EC
Removes polar organic 150 mg MgSO4 150 mg MgSO4
acids, some sugars, 5982-5122 5982-5121
more lipids and sterols 5982-5122CH 5982-5121CH
15 mL 50/pk 400 mg PSA 150 mg PSA
400 mg C18EC 150 mg C18EC
5982-5158 5982-5156
5982-5158CH 5982-5156CH
Pigmented fruits 2 mL 100/pk 50 mg PSA 25 mg PSA
and vegetables: 50 mg GCB 2.5 mg GCB
Removes polar organic 150 mg MgSO4 150 mg MgSO4
acids, some sugars 5982-5222 5982-5221
and lipids, and carotenoids 5982-5222CH 5982-5221CH
and chlorophyll; not for use
with planar pesticides 15 mL 50/pk 400 mg PSA 150 mg PSA
400 mg GCB 15 mg GCB
5982-5258 5982-5256
5982-5258CH 5982-5256CH
Part numbers ending in CH indicate tubes containing ceramic homogenizers.
QUECHERS 231
QuEChERS

AOAC 2007.01 European Method
Method EN 15662
Kit Contents Kit Contents
Kit Size Unit Part No. Part No.
Highly pigmented fruits 2 mL 100/pk 25 mg PSA
and vegetables: 7.5 mg GCB
Removes polar organic 150 mg MgSO4
acids, some sugars and 5982-5321
lipids, plus high levels of 5982-5321CH
carotenoids and chlorophyll;
not for use with planar 15 mL 50/pk 150 mg PSA
pesticides 45 mg GCB
900 mg MgSO4
5982-5356
5982-5356CH
Fruits and vegetables 2 mL 100/pk 50 mg PSA
with pigments and fats: 50 mg GCB
Removes polar organic 150 mg MgSO4
acids, some sugars and 50 mg C18EC
lipids, plus carotenoids 5982-5421
and chlorophyll; not for use
with planar pesticides 5982-5421CH
15 mL 50/pk 400 mg PSA
400 mg GCB
1200 mg MgSO4
400 mg C18EC
5982-5456
5982-5456CH
Other Food Methods 2 mL 100/pk 25 mg C18
Removes biological matrix 150 mg MgSO4
interferences, including 5982-4921
hydrophobic substances 5982-4921CH
(fats, lipids) and proteins
15 mL 50/pk 150 mg C18
900 mg MgSO4
5982-4956
5982-4956CH
All Food Types 2 mL 100/pk 50 mg PSA
Removes virtually all matrix 50 mg C18
interfering materials 7.5 mg GCB
including polar organic 150 mg MgSO4
acids, lipids, sugars, 5982-0028
proteins, carotenoids 5982-0028CH
and chlorophyll
15 mL 50/pk 400mg PSA
400 mg C18
45 mg GCB
1200 MgSO4
5982-0029
5982-0029CH
Part numbers ending in CH indicate tubes containing ceramic homogenizers.

QuEChERS
QuEChERS Ceramic Homogenizers

Ceramic homogenizers increase your overall lab productivity and give you greater confidence
in your results. They make analyte extraction easier by:
• Cutting the required extraction time from 60 seconds to as little as 20 seconds –
a time savings of 70% per sample
• Maintaining high, reproducible extractions in a third of the time
• Minimizing variance between technicians
• Breaking up salt agglomerates and maintaining a consistent grinding
of homogenizing material
• Increasing your overall lab productivity and having greater confidence in your results
The same great ceramic homogenizers available in our QuEChERS Kits are also available for bulk
purchase, providing excellent grinding capabilities of the samples.
QuEChERS Ceramic Homogenizers

Ceramic homogenizer for 50 mL tubes 100/pk 5982-9313
QuEChERS Bulk Sorbents and Salts

If you prefer to pack your own tubes for QuEChERS, use these high-quality bulk sorbents
and salts.
QuEChERS Bulk Sorbents and Salts

Magnesium Sulfate 100 g bottle 5982-8082
Sodium Acetate 100 g bottle 5982-5751
Sodium Chloride 100 g bottle 5982-5750
PSA (Primary Secondary Amine) 25 g bottle 5982-8382
100 g bottle 5982-5753
C18EC 25 g bottle 5982-1382
100 g bottle 5982-5752
Graphitized Carbon Black (GCB) 25 g bottle 5982-4482
Si-SAX 25 g bottle 5982-2082
QUECHERS 233
QuEChERS
Standards for QuEChERS Products

In addition to our industry-leading SampliQ QuEChERS Kits, Agilent makes your analysis easier
by providing standards for the most commonly used regulatory methods, including AOAC and EN.
• Save time and inconvenience of making standards

• Available for both GC and LC instruments
• Ready to use for QuEChERS extractions – no dilutions required
Standards for QuEChERS Products

Description Concentration Kit Contents Part No.
HPLC & GC Internal Standard, 1000 μg/mL Parathion-d10 (diethyl-d10), Alpha-BHC-d6 (alpha-HCH-d6) 5190-0502
AOAC Method
QC Solution, AOAC Method 500 μg/mL Triphenyl phosphate 5190-0503
HPLC Internal Standard, EN Method 100 μg/mL Tris (1,3-dichloroisopropyl) phosphate, Nicarbazin 5190-0500
GC Internal Standard, EN Method 5000 μg/mL (2,2'5,5'-tetrachlorobiphenyl), Triphenylmethane, 5190-0501
Tris (1,3-dichloroisopropyl) phosphate
QC Surrogate for GC Standard, 500 μg/mL (2,2',3,4,4',5'-hexachlorobiphenyl) 5190-0499
EN Method 1000 μg/mL Anthracene-d10
GC Standard Mix, EN Method 100 μg/mL Malathion, Methyl parathion, Parathion (ethyl), Chlorpyriphos, Fenitrothion, 5190-0497
Dichlorvos, Deltamethrin, Chlorpyriphos-methyl, Heptachlor, Bromopropylate,
Gamma-HCH, Aldrin, Dieldrin, Disulfoton, Fenvalerate, Procymidone,
Hexachlorobenzene, Lamda-cyhalothrin, 4,4'-DDT, Endosulfan I, Endosulfan II,
Endosulfan sulfate, 4.4'-DDE, 4,4,-DDD, Alpha-BHC, Beta-BHC
For more information on QuEChERS, download the brochure at

www.agilent.com/chem/quechersbrochure

Captiva Filtration
CAPTIVA FILTRATION
Captiva's unique dual-depth filtration media provides complete removal of precipitated proteins
and outstanding resistance to sample clogging, with no loss of analytes. All Captiva components
are ultra clean, and rigorously tested to ensure against non-specific binding. With Captiva, your
plasma samples are processed quickly and reliably. Captiva is easily automated for enhanced
productivity and excellent for sample storage.
Time-consuming sample transfer steps required with conventional precipitation are now
a thing of the past. With Captiva, clean, clear filtrates are ready for injection in minutes –
this user-friendly filtration device is simple and streamlined with an easy-to-follow 3-step process.
And because Captiva samples are pellet-free, you can sample directly from the collection plate.
The Captiva range includes:
• Captiva NDLipids, the non-drip filtration plate for lipid and protein depletion
• Captiva 96-well filter plates for preparing precipitated proteins for LC/MS
• Captiva filter cartridges, all the usual Captiva benefits in a standard SPE cartridge format
CAPTIVA FILTRATION 235

Captiva Filtration
Tips & Tools Captiva NDLipids

Using Captiva NDLipids with methanol • More precise and reproducible quantitation with removal of phospholipids and proteins
is an excellent replacement for • Increased productivity due to extended column lifetimes and cleaner MS ion sources
acetonitrile as the precipitation solvent. • Simple 3-step procedure
Methods with methanol show better
removal of lipids than with acetonitrile. Capitiva NDLipids is as simple and easy-to-use as a standard protein precipitation plate.
Converting to methanol is advantageous The non-drip 96-well filtration plate is specially designed to effectively remove phospholipids
when the supply or cost of acetonitrile from biofluids. Captiva NDLipids removes lipids, proteins, surfactants and other matrix
is restrictive. Methanol can now be interferences from plasma extracts. Ion suppression is significantly reduced for enhanced
your solvent of choice for lipid removal. sensitivity and precision during trace analysis. The depletion of lipid compounds also gives
you better peak shapes and reproducible retention times so that standard operating procedures
are easily validated. In addition, the fast, in-well precipitation technology of Captiva NDLipids
ensures high sample throughput and helps reduce instrument downtime, with virtually no
need for method development on a wide range of analytes.
Captiva NDLipids
Captiva NDLipids 96-well filtration starter kit A59640002SK
Includes 1 CaptiVac vacuum collar, 2 Captiva NDLipids filter plates,
2 Captiva 96 deep-well 1 mL collection plates and 2 Captiva collection plate
pierceable covers
Captiva NDLipids 96-well filtration replacement kit A59640002RK
Includes 2 Captiva NDLipids filter plates, 2 Captiva 96 deep-well 1 mL collection
plates and 2 Captiva collection plate pierceable covers
Captiva NDLipids 96-well filter plate, 1 mL well A59640002I
Captiva NDLipids 96-well filter plates, 1 mL well, 5/pk A59640002V
DuoSeal 96 96-well plate seals, 10/pk A8961008

Captiva Filtration
Simple and Easy-to-Use Post-column infusion of albuterol before treatment with Captiva NDLipids
Captiva NDLipids Note that the ion-suppression features (top trace) correlate with the elution of phospholipids (bottom trace).
100
Easy 3-Step Procedure PCI
Norm (%)
1. Add precipitation solvent
and plasma sample in desired ratio
into the 96-well plate
2. Precipitate 0
MS lipid trace
3. Filter 0 min 35
Same experiment after protein and lipid depletion with Captiva NDLipids
Key Salts 100
Proteins
Lipids PCI
Norm (%)
Analyte
1
0 MS lipid trace
0 min 35
CAPTIVA_01
Ion suppression is dramatically reduced and the lipids are almost non-detectable.
Longevity Study Illustrating Prolonged Column Lifetime

When Using Captiva NDLipids
50 UV
2
Pindolol
10th Injection tr = 0.907 min 2721 psi

Atenolol
Propranolol
Mcounts

3
0.25 min 1.75
50 MS
Atenolol

Propranolol
Pindolol
Mcounts
0.25 min 1.75

CAPTIVA_02
No significant changes in back pressure, retention time, and peak shape with Captiva NDLipids after 10 and 5010
injections for LC/MS or LC/MS/MS bioanalysis (top = UV detection; bottom = MS detection).

Captiva Filtration
Captiva 96-well Filter Kits

• The industry standard for centrifugation-free protein precipitation
• Fast and reliable processing improves productivity
• Starter kits contain everything you need
Faster than centrifugation and easily automated, Captiva's unique dual-depth filtration media
provide complete removal of precipitated proteins and outstanding resistance to sample
clogging. With Captiva, your plasma samples are processed quickly and reliably, and you can
avoid fibrinogen clogging forever. The plates are also excellent for sample storage. All Captiva
components are ultra clean, and rigorously tested to ensure against non-specific binding.
Starter kits contain everything you need to get up and running with minimum fuss.
Replacement kits include everything you need to replenish your Captiva system.
Captiva 96-well Filter Kits

Pore Size (μm) Filter Material Part No.
Starter Kits
0.2 Polypropylene A5960002SK
0.45 Polypropylene A5960045SK
10 Glass fiber A596401000SK
Includes 1 CaptiVac vacuum collar, 5 Captiva filter plates, 10 DuoSeal 96 96-well plate seals,
Captiva 96-well filter kit 5 Captiva 96 deep-well 1 mL collection plates, 5 Captiva collection plate pierceable covers
Replacement Kits
0.2 Polypropylene A5960002K
0.45 Polypropylene A5967045K
Polyvinyldifluoride and polypropylene A5960045K
Includes 5 Captiva filter plates, 10 DuoSeal 96 96-well plate seals, 5 Captiva 96 deep-well
1 mL collection plates, 5 Captiva collection plate pierceable covers

Captiva Filtration
Captiva 96-well Filter Plates

• Protect HPLC columns from clogging to reduce instrument downtime
• Clean and clear filtrates offer improved sensitivity
• High analyte recovery with simple robust methods allows faster method development
Filtration is simple, versatile, and necessary to prevent clogging of valuable HPLC columns.
Captiva 0.2 μm and 0.45 μm depth filter plates are ideal for preparing precipitated protein
samples for LC/MS analysis. Captiva 10 μm and 20 μm glass fiber filter plates are designed
for clarifying highly particle-laden samples, such as freshly thawed plasma and hepatocyte
filtration, preventing sample transfer problems from pipette tip clogging. They are perfect
for automated systems and for use with DuoSeal 96 96-well seals.
Captiva 96-well Filter Plates, 5 x 96 well

Pore Size (μm) Filter Material Part No.
0.2 Polypropylene A5960002
0.45 Polyvinyldifluoride and polypropylene A5967045
Polypropylene A5960045
10 Glass fiber A596401000
20 Polypropylene A596002000
Polypropylene A596002000B
Bulk Pack, 100 x 96 well

Captiva Filtration
Captiva 96-well Collection Plates and Cover

• Designed for Captiva filtration, SPEC and Bond Elut 96 applications
• Standard 1 mL format offers compatibility with further automation or liquid handling
• Silicone cover preserves sample integrity
Captiva 96-well collection plates are specially designed for use with Captiva filtration plates,
SPEC SPE 96-well plates and Bond Elut 96-well plates. The 1 mL capacity provides the volume
needed to collect all of your filtrate or eluate. Captiva pierceable 96-well silicone covers
are easily applied to completely seal the plates, ensuring no sample loss due to spillage
or evaporation and no sample contamination. The silicone is specially designed for 96-well
auto injectors, providing easy piercing and removal.
Captiva 96-well Collection Plates and Cover

Captiva 96-deep well collection plate, 1 mL 10/pk A696001000
Captiva pierceable 96-well collection plate cover 10/pk A8961007
DuoSeal 96 10/pk A8961008
Captiva 96-well collection plate, A696001000
Captiva Filter Cartridges

• Standard SPE format
• Ideal for LC/MS samples
• Avoid sample transfer problems
Captiva filter cartridges bring all of the benefits of Captiva filtration to the standard SPE cartridge
format. The 0.2 μm and 0.45 μm filter cartridges are ideal for preparing precipitated protein
samples for LC/MS analysis. The Captiva 10 μm glass fiber filter cartridge is designed for
clarifying highly particle-laden samples, such as freshly thawed plasma, preventing sample
transfer problems due to pipette tip clogging.
Captiva Filter Cartridges

Pore Size (μm) Filter Material Volume (mL) Unit Part No.
0.2 Polyvinyldifluoride and polypropylene 3 100/pk A5300002
0.45 Polyvinyldifluoride and polypropylene 3 100/pk A5307045
10 Glass fiber 10 100/pk A500401000
Captiva filter cartridges, glass fiber, A500401000

Captiva Filtration
CaptiVac Vacuum Collars

• Pre-aligned for trouble-free operation
• Vacuum sealed for maximum efficiency
• Simple, cost effective solution
For use with Captiva Filtration and SPEC 96-well Plates, this patented vacuum collar is a
completely transparent device that joins Captiva or SPEC plates directly onto our collection
CaptiVac vacuum collar, A796 plate. The unique design of the Captiva collar forms a pre-set, pre-aligned vacuum seal between
the filtration and collection plate, which positions the outlet tips at a specified distance inside
each well, so as to prevent cross contamination of samples.
CaptiVac Vacuum Collars

CaptiVac vacuum collar A796
CaptiVac gasket kit, 5/pk A796G

Sample Filtration
SAMPLE FILTRATION
Various methods of sample filtration can be used to clarify samples that need further analysis
or where particulate matter may cause a problem. Agilent provides a range of standard
and economy syringe filters conveniently housed in inert polymeric housings for easy use
and disposal, as well as the innovative Mini-UniPrep Syringeless Filters from Whatman.
Syringe Filters
Membrane syringe filters are used most often to clarify small-volume sample solutions prior
to HPLC and ion chromatography. The membrane filters are contained in an inert polymeric
housing. No glue or binders are used in their construction to ensure that no extractables
are present. The housing is designed to spread the sample solution over the entire surface
of the membrane so that maximum membrane capacity is used. Syringe filters are ready-to-use
2-in-1 Filter and are quite convenient. You merely attach the Luer-tipped syringe filled with the sample
solution to the housing and push the sample through the pre-cleaned filters.
How to Select the Right Membrane Syringe Filter

Why Filter Your Samples Filter types should be selected based on sample volume. All filter inlets are female Luer-
for HPLC? compatible, have inert polypropylene or polycarbonate housings and come in three diameters:
• To protect your column against • 30 mm filters are designed for larger sample volumes or for solvent filtration and offer
plugging (blockage) from sample increased filtration speed. The holdup volume is less than 50 μL.
particulate matter
• To protect your injection valve • 25 mm Econofilters offer a moderately wide cross-sectional area (4.2 cm2) with a holdup
components from possible damage, volume of less than 50 μL.
scratching and increased wear by
sample particulate matter
• 13 mm filters are ideal for most applications and offer the best compromise between holdup
volume and sample volume. Sample volumes are typically in the 1-10 mL range and the
• To minimize downtime holdup volume is less than 10 μL.
Porosity should be determined by the size of potential particulates in your sample. Finer porosities
require more pressure to filter.
Two porosities are available: 0.45 μm pores to remove particles that are detrimental to most
columns and 0.20 μm pores to remove the smallest particles.

www.agilent.com/chem/supplies
Sample Filtration
Membrane Filters
When selecting membrane filters, solvent compatibility is the most important criterion.
The chemical resistance table lists the most popular solvents used in HPLC along with
the compatible membrane types.
Recovery of polynuclear aromatic hydrocarbons
% Recovery μg/cm2
100 100 92.4 Regenerated
cellulose
Agilent Regenerated Cellulose

80 80 72.2 Cellulose acetate
Agilent Cellulose Acetate

60 60
Cellulose Nitrate
40 40 32.3
Polyamide
20 20
PVDF
2.7 1.79
0 0
Regenerated cellulose membranes are recommended for general HPLC sample preparation, as well as filtration of aqueous biological samples and organic solvents.
PTFE membranes are compatible with almost all solvents, acids and bases.
Cellulose nitrate is primarily used for pre-filters.
Cellulose acetate membranes are not compatible with organic solvents. They are specially recommended for proteins and protein-related samples.
Why Purchase Agilent Syringe Filters?

Agilent offers the most popular sizes, porosities
and membrane types at attractive pricing. All of our
syringe filters are pre-sterilized by gamma irradiation,
and our regenerated cellulose filters are batch-tested
under HPLC conditions.
SAMPLE FILTRATION 243

Sample Filtration
Chemical Resistance Table for Membrane Filters

Substances Membrane filters
Cellulose nitrate Cellulose acetate Regenerated cellulose Nylon PTFE
1,4-Dioxane - - + N/A 0
Acetic acid, 10% & 25% + 0 + - +
Acetone - - + + +
Acetonitrile - - + + -
Alcohols (i-Propanol, 1-Hexanol, Cyclohexanol) + + + + +
Aliphatic hydrocarbons + + + + +
Aromatic hydrocarbons + + + N/A +
Carboxylic acid + + + - +
Cyclohexane 0 0 + + +
Diethylether 0 0 + + 0
Dimethyl formamide - - 0 + +
Dimethyl sulfoxide - - 0 N/A +
Ethanol = <98% - + + + +
Ethyl acetate - - + + +
Formic acid, 25% + 0 + - +
Hexane + + + + +
Hydrochloric acid, 25% + - + - +
Methanol - + + 0 +
Nitric acid, 25% 0 0 + - +
Phosphoric acid, 45% 0 0 0 0 +
Potassium hydroxide, 1 M - - 0 - +
Salt solutions, aqueous + + + + +
Sodium hydroxide, 1 M - 0 - - +
Tetrahydrofuran - - + + 0
Toluene + + + + +
Trichloroacetic acid, 10% + - - 0 +
Trichloroethane + 0 + 0 +
Xylene + + + + +
Code for Table: + = Resistance, 0 = Limited resistance, - = Not resistant, N/A = Not available

Sample Filtration
Premium Syringe Filters

Agilent premium syringe filters are high-quality, ready-to-use, tested and certified for the absence
of UV-absorbing substances at typical HPLC wavelengths with water, methanol and acetonitrile.
Premium Membrane Filters, 100/pk

Description Diameter (mm) Pore Size (μm) Part No.
PTFE with Luer tip 30 0.2 3150-0753
PTFE with mini tip 13 0.45 5185-5837
Cellulose Acetate with Luer tip 30 0.45 5061-3363
Premium Regenerated Cellulose Membrane Filters, 100/pk

Luer tip 30 0.2 5061-3354
Luer tip 30 0.45 5061-3364
Luer tip 13 0.2 5064-8222
Luer tip 13 0.45 5064-8221
Mini tip 13 0.2 5061-3366
Regenerated cellulose filters, 5061-3364
Luer tip 13 0.45 5061-3365
Econofilters
High quality econofilters are shipped in large packs and are ideal for busy labs that need fast,
efficient filtration at a reasonable price.
Membrane Econofilters, 200/pk

Regenerated cellulose 25 0.2 5185-5830
Regenerated cellulose 25 0.45 5185-5831
Membrane Econofilters, 5185-5830 Nylon 25 0.2 5185-5832
Nylon 25 0.45 5185-5833
PTFE 25 0.2 5185-5834
PTFE 25 0.45 5185-5835

Sample Filtration
Pre-Filters (Standalone)
Pre-filters have a 100% borosilicate glass fiber membrane that is chemically inert and resistant
to most solvents. The high surface area of the rigid fiber structure provides outstanding particle
retention capacity while maintaining low flow resistance. The GF-53 will retain coarse particles
down to approximately 3 μm in diameter and the GF-92 down to approximately 2 μm in
diameter. They can be used standalone or in a series with a membrane filter.
Pre-Filters (Standalone), 100/pk

Glass fiber (GF-53) 5042-1393
Glass fiber (GF-92) 5042-1370
2-in-1 Filters
2-in-1 filters are recommended for difficult-to-filter samples because they have a two-layered
filter in a single housing. The coarse, top layer pre-filter removes larger particulates before
getting to the membrane filter, requiring less force to push liquid sample through the filter,
thereby providing higher throughput, and saving time, sample and money.
2-in-1 Filters, 100/pk

Glass Fiber/Cellulose Nitrate 30 0.45 5042-1391
Glass Fiber/Regenerated Cellulose 30 0.45 5042-1392
Syringes for Sample Filters, 10/pk

Disposable syringes, 20 mL 5062-8534

Sample Filtration
Mini-UniPrep Syringeless Filters

Agilent is pleased to offer Mini-UniPrep Syringeless Filters from Whatman. The Mini-UniPrep is
a pre-assembled, disposable filtration device, ideal for removing particulate matter from samples.
This small filter performs the functions of syringe filters, disposable syringes, vials, septa and caps
in one small package, and protects your valuable HPLC column from contamination.
• Ideal for samples that undergo demanding HPLC analysis

• Equivalent in size to standard 12 x 32 mm vial, with a filtering capacity of 0.5 mL
• Innovative design reduces costs, materials, time to prepare samples,
and is environmentally-responsible
• Compatible with all Agilent 1100 and 1200 Series autosamplers and manual injectors
Mini-UniPrep Syringeless Filters, 100/pk

0.45 μm PTFE 5190-1415
For aggressive samples
0.45 μm Nylon 5190-1416
For aqueous and organic samples, pH 3-10
0.45 μm PP 5190-1417
For solvent-based samples, low water breakthrough values
0.45 μm regenerated cellulose 5190-1418
For aqueous or organic solvents, very low non-specific protein binding membrane
0.20 μm PTFE 5190-1419
For aggressive samples
0.20 μm Nylon 5190-1420
For aqueous and organic samples, pH 3-10
0.20 μm PP 5190-1421
For solvent-based samples, low water breakthrough values
0.20 μm regenerated cellulose 5190-1422
For aqueous or organic solvents, very low non-specific protein binding membrane
Tips & Tools

For more information, including chemical compatibilities,
visit www.agilent.com/chem/miniuniprep

TOXI-TUBES
TOXI-TUBES
• Boost LC/MS and GC/MS efficiency with fast, simple drug extractions
• Reduce risk of system downtime and increase throughput by reducing column changes
• Quick, easy, clean extraction of drugs from samples
Use the quickest, easiest, and cleanest liquid/liquid extraction procedure available to increase
your GC/MS and LC/MS efficiency. TOXI-TUBES give you a clean one-step process for extracting
a broad range of drugs from biological or non-biological specimens.
TOXI-TUBES
Description Use With Unit Part No.
TOXI-TUBES A Organic bases 100/pk A109A100
Pre-measured solution of buffering salts (pH 9) and neutral drugs
and organic solvents
TOXI-TUBES B Acidic 100/pk A109B100
Pre-measured solution of buffering salts (pH 4.5) and neutral drugs
and organic solvents

TOXI-TUBES
Selected profiles of five benzodiazepines extracted from a whole blood

sample using TOXI-TUBES A. Each drug was spiked at 50 ng/mL in blood.
Nordiazepam
Oxazepam
Bromazepam
Flunitrazepam
Prazepam
Benzodiazepine Recovery Ratio (%)

Whole Blood Urine
Nordiazepam 75.5 88.2
Oxazepam 72.0 85.9
Bromazepam 67.8 91.2
Flunitrazepam 72.7 89.8
Prazepam 74.7 93.2

THEEssential
CHROMATOGRAPHY & SPECTROSCOPY
CATALOG
This information is subject to change without notice.
11
© Agilent Technologies, Inc. 2011
12
EDITION
INTRODUCTION
PAGES 1-33

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THEEssential

CHROMATOGRAPHY & SPECTROSCOPY

140 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Solid Phase Extraction (SPE)

SOLID PHASE EXTRACTION (SPE) 141

142 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

SOLID PHASE EXTRACTION (SPE)

Agilent Bond Elut:

The Bond Elut Difference

SOLID PHASE EXTRACTION (SPE) 143

Sample Preparation Formats

Agilent Offers the Broadest Range

Diagrams are to scale

144 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Bond Elut 96-well Plates

Packed Formats for Automation

10 mL LRC 6 mL 3 mL 1 mL Bond Elut Jr

SOLID PHASE EXTRACTION (SPE) 145

Cross Reference of Comparable Phases by Manufacturer

Silica-Based and Other Sorbents

146 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

SOLID PHASE EXTRACTION (SPE) 147

148 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Particle Size Specifications

SOLID PHASE EXTRACTION (SPE) 149

Sample Preparation Reference Guide

150 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Sample Preparation Reference Guide

SOLID PHASE EXTRACTION (SPE) 151

Bond Elut Plexa

Bond Elut Plexa

Bond Elut Plexa PCX

Bond Elut Plexa PAX

152 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Advanced Polymer Architecture Improves Extraction Performance

LOAD: WASH: ELUTE:

Tips & Tools

To learn more, visit www.agilent.com/chem/OnlineLibrary

SOLID PHASE EXTRACTION (SPE) 153

General Protocol for Trouble-Free SPE Applications

Acids Neutrals Bases

Log P > 1.0 Log P > 1.5 Log P > 0.8

Plexa Acid Plexa Base

Sample Treatment 2% NH4OH 1% HCO2H 2% NH4OH 2% H3PO4

Sorbent Condition 100% MeOH 100% MeOH 100% MeOH

Equilibrate 100% H2O 100% H2O 100% H2O

Wash 100% H2O 5% MeOH in H2O 2% HCO2H in H20

Elution 1 100% MeOH 100% MeOH 1:1 MeOH/ACN

154 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Comparison of particle sizes of non-polar SPE polymers

Metoprolol recovery calculated as

Metoprolol recovery calculated as Bond Elut Plexa PCX Alternative Polymer

Comparison of particle size distributions of non-polar SPE sorbents

SOLID PHASE EXTRACTION (SPE) 155

Bond Elut Plexa

Typical Matrices Primary Extraction Mechanism Compound Types

156 SAMPLE PREPARATION www.agilent.com/chem/samplepreparation

Bond Elut Plexa

SOLID PHASE EXTRACTION (SPE) 157