NH3 BX400 Tyb40 Rximmola Draft 01

Download as pdf or txt
Download as pdf or txt
You are on page 1of 3

NH3

AMONIACO
Enzimático-UV

BX_400 / TYB_40 / RX_IMMOLA


PREPARACIÓN Y ESTABILIDAD
Ref. 1001410.
• RT1 Reconstituir el contenido de un vial de R1a con 5 mL de R1b tampón. El Reactivo de Trabajo 1 es estable 5
días a 15-25ºC o 3 semanas a 2-8ºC.
• R2 Diluir el contenido del vial de R2 con 20 mL de R1b.

CALIBRACIÓN
Utilizar un suero de calibración adecuado.

CONDICIONES OPERATORIAS
Introducir en la memoria del ordenador los siguientes parámetros:

CHEMISTRY PARAMETER

Method * Name NH3 Reagent Name Reagent (µL) Water (µL)


Print Name NH3 R1 NH3 220
Sample NH3 R2 X Enable NH3 60
Unit µmol/L
Assay Type Fixed Time Diluent Enable

Mesuring Points Decimal Points 1


Start End
1 43 80 Normal Range
Enable 2 Nº Range Name Min. Max.
1 * * *
Wave Lenght 2 * * *
Prim. 340 Sec x Disable 3 * * *
4 * * *
Normal
Sampling Sample (µL) Diluent (µL) LOW HIGH
Dilution 25 Technical (Conc.) * *
Rerun (High Prozone) Range (mAbs/10) -999999 999999
Dilution
Rerun (Low) SPT Wash Enable Reag. Name
Dilution Stirring Speed R1-Middle R2-Middle

Limit Checks Blank measurement


Duplicate Limit mAbs/10
Sensitivity Limit mAbs/10 Blank measurement Enable Reagent Bk for S1 (linear)
Linearity Limit % (mAbs/10)/min Meas. of reagent BK during run Next Run
Prozone Limit Reag. BK. meas. at calibration Reagent BK (system water)
SL1-S SL1-F Nº of measurement Duplicate
SL2-S SL2-F
SENS mAbs/10 Reagent Blank Limit Checks:
Duplicate Limit mAbs/10
x Absorbance limit
Reaction Decrease Instrument Factor
Limit * mAbs/10 A: 1.00 B: 0.00

Calibration: 2 linear point.

REV.01 Ed. 2023/08

SPINREACT, S.A.U. Ctra. Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com
NH3

AMMONIA
Enzymatic-UV

PREPARATION AND STABILITY BX_400 / TYB_40 / RX_IMMOLA


Ref. 1001410.
• WR1 Reconstitute the contents of one vial R1a with 5 mL Buffer R1. The working reagent 1 is stable 5 days
at 15-25ºC or 3 weeks at 2-8ºC.
• R2 Dilute the contents of one vial R2 with 20 mL Buffer R1b.

CALIBRATION
Use the calibrator included in the kit.

OPERATORY CONDITIONS
Introduce the following data in the computer:

CHEMISTRY PARAMETER

Method * Name NH3 Reagent Name Reagent (µL) Water (µL)


Print Name NH3 R1 NH3 220
Sample NH3 R2 X Enable NH3 60
Unit µmol/L
Assay Type Fixed Time Diluent Enable

Mesuring Points Decimal Points 1


Start End
1 43 80 Normal Range
Enable 2 Nº Range Name Min. Max.
1 * * *
Wave Lenght 2 * * *
Prim. 340 Sec x Disable 3 * * *
4 * * *
Normal
Sampling Sample (µL) Diluent (µL) LOW HIGH
Dilution 25 Technical (Conc.) * *
Rerun (High Prozone) Range (mAbs/10) -999999 999999
Dilution
Rerun (Low) SPT Wash Enable Reag. Name
Dilution Stirring Speed R1-Middle R2-Middle

Limit Checks Blank measurement


Duplicate Limit mAbs/10
Sensitivity Limit mAbs/10 Blank measurement Enable Reagent Bk for S1 (linear)
Linearity Limit % (mAbs/10)/min Meas. of reagent BK during run Next Run
Prozone Limit Reag. BK. meas. at calibration Reagent BK (system water)
SL1-S SL1-F Nº of measurement Duplicate
SL2-S SL2-F
SENS mAbs/10 Reagent Blank Limit Checks:
Duplicate Limit mAbs/10
x Absorbance limit
Reaction Decrease Instrument Factor
Limit * mAbs/10 A: 1.00 B: 0.00

Calibration: 2 linear point

REV.01 Ed. 2023/07

SPINREACT, S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com
NH3

REV.01 Ed. 2023/07

SPINREACT, S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99 e-mail: spinreact@spinreact.com

You might also like