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    Test For Starch, Protein & Fat

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    juanfateen5888

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    Test For Starch, Protein & Fat

    Uploaded by

    juanfateen5888
    49 views5 pages

    Original Title

    Test for Starch,Protein & Fat

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    © All Rights Reserved
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    Download as pdf or txt
    49 views5 pages

    Test For Starch, Protein & Fat

    Uploaded by

    juanfateen5888

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Test for Starch

    Aim:- The iodine test for starch is mainly performed to test the presence of

    carbohydrates.
    Materials Required

     Knife
     Spatula
     Porcelain tile.
     Iodine solution
     Food sample – Potato or any other vegetables or fruits.

    Procedure

     Take a fresh Potato which is washed, cleaned and dried.


     Peel off the skin of the potato.
     Cut the potato into small cubes or slices.
     With the help of clean and dried Spatula, place the potato samples on
    the clean and dried porcelain tile.
     Add 2 to 3 drops of dilute iodine solution on the potato samples.
     Keep the slide undisturbed and observe the changes.

    Tabulation:
    Sl.No Sample Colour change Inference/Result
    1. A
    2. B
    3. C

    Observations

    There will be a change in colour. A blue-black colour develops on the slice or


    cubes of the potato samples.

    Result

    The result is positive.According to the observation the food sample or the


    potato slice turned to blue-black on adding the iodine solution. This proves the
    presence of starch in the given plant source.
    Test for Sucrose
    Aim:
    The presence of sucrose can be tested in a sample using Benedict’s test.

    Material Required

     Benedict’s Reagent
     Sugar cane extract
     Concentrated HCl
     NaOH solution
     Burner
     Dropper
     Test tube
     Test tube holder

    Procedure

     Take a clean and dried test tube and add sugar cane extract into it.
     Now carefully add a few drops of concentrated HCl using a dropper to
    the test tube.
     Hold the test tube securely with the help of a test tube holder.
     Place the test tube near the Bunsen burner and allow the solution to
    boil for two minutes.
     While boiling, the hydrolysis of sucrose occurs and the fructose
    converts to glucose.
     With the help of a dropper, add a few drops of NaOH solution to the
    test tube so that the solution turns alkaline.
     Now add a few drops of Benedict’s reagent with the help of a dropper
    into the test tube.
     With the help of a test tube holder, place the test tube near the Bunsen
    burner and allow the solution to boil for a few minutes.
     Observe the changes.

    Tabulation:
    Sl.No Sample Colour change Inference/Result
    1. A
    2. B
    3. C
    Observation

    The colour of the solution colour from blue colour to green colour. From green
    colour, it finally changes to brick red or orange colour. This indicates that the
    solution contains glucose.

    Test for Proteins


    Aim: The presence of proteins in a sample can be detected by the following
    tests:

     Biuret Test
     Xanthoproteic Test
     Million’s Test
    Biuret Test

    Material Required

     1% CuSO4
     40% NaOH solution
     Dropper
     Egg Albumin/ cow Milk
     Test tube
     Test tube holder

    Procedure

     Take a clean and dried test tube and add egg albumin/Milk into it.
     Now add a few drops of 40% NaOH solution with the help of a dropper
    into the test tube containing the egg albumin.
     With the help of a dropper, add 2 to 3 drops of 1% CuSO4 solution into
    the same test tube contained in the egg albumin.
     Now shake the test tube slowly in order to mix the solution present in it
    completely.
     Keep the test tube undisturbed and allow the mixtures to stand for 5
    minutes. After a few minutes, observe the changes.
    Tabulation:

    Sl.No Sample Colour change Inference/Result


    1. A
    2. B
    3. C

    Observation

    The solution in the test tube appears to be violet in colour. This indicates that
    the sample that is tested contains proteins.

    Test for Fats


    Aim: The presence of fats in a sample can be detected by the following tests:

     Sudan III Test


     Paper Spot Test
    Sudan III Test

    Materials Required

     Sudan III Solution


     Oil
     Test tube
     Dropper
     Egg Albumin

    Procedure

     Take a clean and dried test tube and add a few drops of oil into it.
     Now into the same test tube, add five to six drops of the Sudan III
    reagent with the help of a dropper.
     Stir the test tube continuously and allow the solution to stand for a
    while.
     After a few seconds, observe the changes.
    Tabulation:

    Sl.No Sample Colour change Inference/Result


    1. A
    2. B
    3. C

    Observation

    The presence of fat in the sample is indicated when pink colour droplets
    appear on the test tube.

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