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    Exploring Micros

    Uploaded by

    Jacob Himawa

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    © All Rights Reserved
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    Exploring Micros

    Uploaded by

    Jacob Himawa
    11 views7 pages

    Original Title

    EXPLORING MICROSCOPY

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    © © All Rights Reserved

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    Copyright:

    © All Rights Reserved
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    Download as pdf or txt
    11 views7 pages

    Exploring Micros

    Uploaded by

    Jacob Himawa

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 7

    THE MICROSCOPE

    EXPLORING MICROSCOPY

    • Light microscope extends our vision a thousand times, hence objects as small
    as 0.2 micrometer in diameter can be seen.
    • Historically, the microscope had its beginning when the Janssen brothers of
    Holland(1950) constructed a system of lenses that permits observation of minute
    objects.
    • Anton Van Leeuwenhoek made extensively use of this tool. He was called the Father of
    Microscopy.
    KINDS OF MICROSCOPE

    • Light Microscope – use ordinary light where visible light is passed thru the specimen and
    thru glass lenses. The lenses refract the light in such a way the image is magnified as it is
    projected into the eye.
    • Electron Microscope
    • Transmission Electron microscope- uses electromagnets as lenses to focus and magnify mainly the
    internal ultrastructure of the cells.
    • Scanning Electron microscope- useful for detailed study of the surface of the specimen
    • Dark Field Microscope- the object are illuminated only from the sides and light is
    reflected off the object being observed. The object here appears bright against a black
    background.
    • Phase Contrast Microscope- takes advantage of slight differences in refractive index by
    converting these striking brightness difference in the visible image of the cell.
    • Binocular Dissecting Microscope- a three dimensional view of the specimen could be
    observed. Less magnification.
    FACTORS OF MICROSCOPY

    • Magnification- refers to the magnitude of amplification of the image of the specimen by


    the microscope.
    • Resolution- measure of the smallest object visible under the particular objective.
    Distinguish two adjacent points as distinct and separate.
    • Numerical Aperture- index of light gathering power of the objective. It is inversely
    proportional to the limit of resolution.
    • Refractive Index- ability of the medium to deflect light.
    • Working Distance- refers to the distance between the specimen and the objective lens
    when focusing the specimen.
    • Field of Vision- refers to the area visible under the microscope.The LPO has a
    measurement of 1.5 mm while the HPO is 0.35mm in diameter.
    • Depth of Focus- refers to the property of the microscope that tells how deep is the
    dimension.
    USE THIS LINK AS A REFERENCE GUIDE

    • https://www.youtube.com/watch?v=4QoaMVJvc34&t=712s

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