Sustainability 10 01730 With Cover
Sustainability 10 01730 With Cover
Sustainability 10 01730 With Cover
Article
Shih-Wu Liang, Wei-Ta Fang, Shin-Cheng Yeh, Shiang-Yao Liu, Huei-Min Tsai, Jui-Yu Chou and
Eric Ng
https://doi.org/10.3390/su10061730
sustainability
Article
A Nationwide Survey Evaluating the Environmental
Literacy of Undergraduate Students in Taiwan
Shih-Wu Liang 1 , Wei-Ta Fang 2, *, Shin-Cheng Yeh 2 , Shiang-Yao Liu 3 ID
, Huei-Min Tsai 2 ,
Jui-Yu Chou 4 and Eric Ng 5
1 Department of Public Relations and Advertising, Shih Hsin University, Taipei 231, Taiwan;
wuu@mail.shu.edu.tw
2 Graduate Institute of Environmental Education, National Taiwan Normal University, Taipei 116, Taiwan;
scyeh@ntnu.edu.tw (S.-C.Y.); hmtsai@ntnu.edu.tw (H.-M.T.)
3 Graduate Institute of Science Education, National Taiwan Normal University, Taipei 116, Taiwan;
liusy@ntnu.edu.tw
4 Department of Biology, National Changhua University of Education, Changhua 500, Taiwan;
jackyjau@cc.ncue.edu.tw
5 School of Management and Enterprise, University of Southern Queensland, Queensland 4350, Australia;
eric.ng@usq.edu.au
* Correspondence: wtfang@ntnu.edu.tw; Tel.: +886-2-7734-6558
Received: 17 April 2018; Accepted: 23 May 2018; Published: 25 May 2018
Abstract: The aim of this nationwide survey was to assess undergraduate students’ environmental
literacy level in Taiwan. A total of 29,498 valid responses were received from a number of selected
colleges and universities in Taiwan, using stratified random sampling method. A total of 70 items
were used to assess the environmental literacy and the results revealed that undergraduate students
had a relatively low level of environmental knowledge and behavior, while a moderate level
of environmental attitudes was attained. The findings also indicated no significant correlations
between knowledge and attitudes or between knowledge and behavior. However, a higher level
of environmental knowledge correlated significantly with a higher degree of pro-environmental
behavior, and a higher level of environmental knowledge correlated with stronger attitudes.
The results also suggested that females outperformed the males in all categories. Results from
this study could contribute towards further relevant policy discussion and decision-making,
curriculum design and development to the improvement of environmental education in the higher
education sector.
1. Introduction
The on-going environmental problems nowadays can be attributed to the increasing population,
economic development and industrialization, pollution, urbanization, and resource depletion globally.
The fundamentals of these on-going problems are predominantly associated with people’s lifestyles
and their extensive activities occurring in the natural surroundings [1,2], which gives rise to the
importance of balancing the relationship between human and natural environments that was already
recognized and supported by the World Commission on Environment and Development in 1987.
The development of environmental awareness, knowledge and skills are considered as essential to help
minimize environmental problems, and environmental education is seen as a key element in creating
an environmentally literate society [3,4]. Through this, a responsible environmental behavior can be
developed and help prevent and minimize environmental problems in a sustainable manner [5,6].
Current environmental education literature reveals a range of prior studies conducted that
consistently related to the development of an environmentally literate citizenry [7,8]. Some of
these areas of studies include: reviews of environmental education literature [9,10], definitions
and frameworks [11,12], purpose and goals [13,14], and responsible environmental behavior [15,16].
However, there are limited studies conducted on a national scale and in the higher education context,
which is the focus of this study.
Elements Components
Cognitive • Knowledge of natural systems
• Knowledge of environmental issues
• Knowledge of appropriate action strategies
Affective • Environmental awareness and sensitivity
• Environmental values
• Decision-making attitude on environmental issues
Behavioral • Intentions to act
• Environmental action strategies and skills
• Involvement in responsible environmental behavior
The key purpose of this study is to examine the undergraduate students’ environmental literacy
level on a nationwide scale in Taiwan by considering the three key environmental literacy elements as
outlined in the literature. Specifically, the following objectives are investigated.
• To assess the level of environmental literacy of undergraduate students in Taiwan on the following
elements: (1) cognitive (knowledge and skills), (2) affective, and (3) behavioral.
• To identify any significant correlations in the undergraduate students’ scores on the three elements.
• To identify information sources from which undergraduate students gather environmental
information.
2.1. Participants
The participants of the study consisted of first-, second-, third-, and fourth-year undergraduate
students in Taiwan. According to the annual report of Education Statistical Indicators published by the
Ministry of Education, the target population was 1,077,396 students from 163 colleges and universities
in Taiwan in the 2012 academic year when this survey was conducted. A sample of 57 colleges and
universities were selected based on a stratified random sampling method with key considerations
given to geographic (22 regions) and demographic (size and level of colleges and universities) stratum.
With an average sampling rate of 3%, a total of 32,321 questionnaires were distributed and surveyed in
the selected colleges and universities, of which 29,498 valid responses were received that represented a
return rate of approximately 91.3%. To determine the representativeness of the sample, the chi-square
(χ2 ) test was used to test between sample and population demographics, which resulted in Pearson
chi-square (χ2 ) being 393.901, and a p value of approximately 0.000. Thus, the sample size of 29,498
was deemed to be representative of the population. The key demographic profiles of the respondents
were briefly outlined in Table 2.
The findings revealed that both male and female were equally represented in this study with a
respond rate of 49.1% and 49.6% respectively. In terms of the year level, first-year students accounted
for 28.4% and this was followed by second-year (25.6%), third-year (23%) and fourth-year (22.4%)
students. While majority of the students lived with their families (40%), other accommodation
arrangements also included outside school rentals (30%) and school dormitory (27.5%). The most
common type of family structure identified was nuclear family (65.9%) with the remaining being three
generations (19.1%) and single-parent families (11.1%).
Sustainability 2018, 10, 1730 5 of 21
• Step 1: More than 30 research papers and articles related to environmental literacy in Taiwan and
abroad [27,31,32,34] were reviewed to establish the item pools to be considered.
• Step 2: Using a similar process by Erdogan and Ok [31], items in the pool were selected in accordance
to the research objectives guided by the definition of each elements and related components.
The table of specifications, and the initially compiled questions items were subsequently prepared
for panel review.
• Step 3: This initially compiled question items were given to a panel of 10 experts from various
areas of specialization, such as environmental education, earth science, geography, and urban
planning, for their formal review and expert opinions. The experts were required to evaluate the
items on their appropriateness, relevance and language used from which content validity has to
be reached. Each question item had received at least 80% agreement by the experts.
• Step 4: The instrument was revised based on the experts’ opinions and feedback, and subsequently
pre-tested with 20 randomly selected undergraduate students. The item analysis with regards
to difficulty and discrimination for knowledge items and the factor analysis for scale items
were conducted to determine the question items to be included in the final version of the
questionnaire survey.
The final questionnaire survey consisted of two main sections; (1) demographic items,
and (2) environmental literacy assessment items. Although there were 12 survey items in the
demographic section, only some items were presented as variables for analysis as shown in Table 1.
As for the environmental literacy assessment section, there were a total of 70 items used to assess the
three main elements (i.e., cognitive, affective, and behavioral) as discussed in the literature.
Sixteen question items were developed for the cognitive element that aimed to assess
undergraduate students’ knowledge and understanding of natural systems, environmental issues,
Sustainability 2018, 10, 1730 6 of 21
and action strategies. Of these 16 questions, nine were True-False questions and remaining seven were
Multiple-Choice questions. Next, 23 items were included in the affective element which sought to
assess undergraduate students’ environmental awareness and sensitivity, values, and decision-making
attitude on environmental issues. These 23 question items were designed in the form of a five-point
Likert-type scale ranging from 1 (strongly disagree) to 5 (strongly agree). The score was reversed
when the question item was presented in a negative manner. Lastly, the behavioral element was
designed to investigate undergraduate students’ intentions to act, their action strategies and skills,
and involvement in responsible environmental behavior. It consisted of 31 question items using a
five-point frequency scale (never, rarely, sometimes, often, always) that focused on undergraduate
students’ environmentally responsible actions, including persuasion, eco-management, consumer and
economic action, and legal and political action.
report a selection of different fit measures, such as chi-squared test and confirmatory factor analysis.
Of the 29,498 valid questionnaires received, only 27,249 were used in the SEM, since the remaining
questionnaires were incomplete and therefore rejected.
3. Results
Table 3. Descriptive information of the sample on club and society participation, and environmental
information search.
Correct Rate
Items
% 95% C. I.
K1. The ultimate goal for natural environment conservation is to attain “Biodiversity”,
41.9 41.3 ≤ p̂ ≤ 42.5
which signifies the utmost variety of different species.
K2. Greenhouse effect is perfect for the earth’s surface temperature to maintain an
41.3 40.7 ≤ p̂ ≤ 41.9
appropriate environment suitable for living organisms.
K3. Excessive burning of coal, oil, and fossil fuels will lead to ozone depletion. 69.2 68.7 ≤ p̂ ≤ 69.7
K4. Having algae in the rivers can help the ecological balance of the river system. 70.9 70.4 ≤ p̂ ≤ 71.4
K5. Which of the following gas type is not a greenhouse gas? 24.1 23.6 ≤ p̂ ≤ 24.6
K6.Which of the following type of power generation is most environmentally friendly? 61.0 60.4 ≤ p̂ ≤ 61.6
K7. Rainforest ecosystem is important to the earth, which of the following statement is not
86.4 86.0 ≤ p̂ ≤ 86.8
a function of tropical rainforests?
K8. What is the major reason contributing to the problems associated with natural disaster
39.0 38.4 ≤ p̂ ≤ 39.6
in Taiwan?
K9. Which of the following statement about information on conservation is correct? 59.8 59.2 ≤ p̂ ≤ 60.4
K10. “Kyoto Protocol” is an agreement signed for global economic development. 67.2 66.7 ≤ p̂ ≤ 67.7
K11. Ancestors like to drink realgar wine as part of their traditional customs in
45.5 45.0 ≤ p̂ ≤ 46.1
Mid-autumn Festival, to protect them from diseases.
K12. The wisdom pass down by our ancestors is adequate to help us cope with the current
85.9 85.5 ≤ p̂ ≤ 86.3
climate problems and environmental changes.
K13. Which of the following statement is not correct about the damage done on the nature
61.0 60.4 ≤ p̂ ≤ 61.6
from acidic rain?
K14. The reason for uneven distribution of the earth’s natural resources is due to the high
61.6 61.0 ≤ p̂ ≤ 62.2
resource consumption in high population countries. Is this statement correct?
K15. The road work maintenance in Saharan Africa is to prevent landslides due to debris
59.1 58.8 ≤ p̂ ≤ 59.7
flows and reduce the loss of life and property. Is this statement correct?
K16. Which of the following icons represent environmental-friendly labelling?
89.3 88.9 ≤ p̂ ≤ 89.7
Please select one correct icon.
The second component, knowledge of environmental issues in the cognitive element was assessed
through three True-False questions (i.e., K10 to K12) and one Multiple-Choice questions (i.e., K13).
The participants answered 64.9% of the questions correctly, suggesting that their environmental
knowledge of social, cultural, political, and economic issues was at a moderate level. In particular, only
Question K12 on “The wisdom pass down by our ancestors is adequate to help us cope with the current
climate problems and environmental changes” achieved a correct response rate greater than 80%,
while the correct response rate of Question K11 was below 50% (refer to Table 4). This indicated that
the undergraduate students’ environmental knowledge about the customary cultural issues (such as
the Chinese tradition of drinking realgar wine during the autumn season to protect themselves from
illness and stay healthy) was generally inadequate.
Sustainability 2018, 10, 1730 9 of 21
When assessing the knowledge of appropriate action strategies component in the cognitive
element, two True-False questions (i.e., K14 to K15) and one Multiple-Choice question (i.e., K16) were
used. The participants answered 61.5% of the questions correctly, revealing that their knowledge of
appropriate action strategies was moderately inadequate. Although Question K16 on the “identification
of environmental labelling” had attained a correct response rate greater than 80%, however, the correct
response rate for Question K15 was below 60% (refer to Table 4). This indicated that undergraduate
students’ knowledge of appropriate action strategies was generally insufficient, particularly regarding
the concern of the “cognitive awareness level of resource consumption ratio”.
Standard
Items Mean
Deviation
A1. I am aware of environmental issues related to air pollution, food safety, and habitat destruction. 3.64 0.874
A2. I care about environmental problems caused by climate change. 3.71 0.849
A3. I believe that toxic emissions from anthropogenic waste (i.e., motor vehicles, factories, etc.) can
4.09 0.957
cause a negative environmental impact.
A4. I think human lives are critically dependent on the supply of the earth’s natural resources. 3.96 0.973
A5. I think the practice of environmentally friendly behavior can solve many environmental
3.58 0.976
problems in lives.
A6. I have the initiative to learn environmental knowledge (i.e., interactions between people and the
3.45 0.844
environment), to enhance my understanding of the natural world.
A7. I will be very angry if I saw someone throw trash on the road or into the river. 3.82 0.932
A8. I will give thanks and cherish “Mother Nature” more for a better environment. 3.93 0.910
A9. Earth’s resources are limited, so I will cherish resources. 3.96 0.920
A10. I think there is a meaning and value for the existence of the plants and trees. 4.06 0.957
Sustainability 2018, 10, 1730 10 of 21
Table 5. Cont.
Standard
Items Mean
Deviation
A11. I think the aboriginal customary manners are worthy of respect and learning, given that they
3.86 0.949
contribute towards a positive ecological relationship.
A12. I think there is no way to restore the original look of the nature if it is destroyed. 4.11 1.018
A13. I think environmental and ecological protection are more important than that of the
3.84 0.951
economic development.
A14. I believe that technological advances cannot solve all the environmental pollution problems. 3.83 1.023
A15. I think the environmental pollution problems in other countries will also affect Taiwan. 4.02 0.951
A16. I think that polyethylene terephthalate bottled drinks affect the environment. 3.83 0.919
A17. I know my lifestyle can affect the environment. 3.68 0.924
A18. I have a responsibility to participate in activities that will help slow down global warming. 3.86 0.909
A19. As long as I am willing to try, I can solve or minimize environmental problems. 3.67 0.936
A20. I can discuss environmental issues with other students to clarify the cause and effects. 3.46 0.900
A21. I will advise others to stop environmental destruction (e.g., dumping trash and waste water, etc.). 3.54 0.897
A22. While traveling through local communities, I will respect local customs and traditions, and to
3.90 0.894
reduce interference on the local environment and residents.
A23. I believe no advanced technology can solve all pollution problems. 3.84 1.35
Standard
Items Mean
Deviation
BEH1. While I go out, I am willing to give priority to walking, cycling, taking public transportation,
3.67 0.966
and other transportation modes.
BEH2. I am willing to turn on air conditioner’s temperature at 26 ◦ C~28 ◦ C to save energy. 3.84 0.988
BEH3. I am willing to take care of our living environment (including school and neighborhood) and
3.59 0.870
make changes to the environmental conditions.
BEH4. I am willing to take the initiative to find out more about climate change, carbon reduction,
3.63 0.853
and other related information.
BEH5. I am willing to take the initiative to follow through in my daily life on the opinions and ideas
3.43 0.861
I have about the environmental issues.
BEH6. I am willing to discuss environmental issues as the main theme in the school report. 3.36 0.876
BEH7. I support the reduction on the number of household garbage collection days, in order to reduce
3.44 0.963
air pollution generated by garbage truck.
BEH8. I am willing to participate in the environmental events (i.e., natural conservation, monitoring,
3.36 0.897
and maintenance activities).
BEH9. I am capable of classifying different types of recyclable items. 3.67 0.926
BEH10. I know how to use different recycling methods. 3.24 0.941
BEH11. I am capable of observing the terrain to determine if a place will be flooded or not. 2.84 1.043
BEH12. I will use multi-media, such as newspapers, magazines, or the Internet to obtain information
3.29 0.974
related to environmental issues.
BEH13. I am able to identify environmental problems and find solutions to them. 3.00 0.964
BEH14. I can integrate different viewpoints on environmental issues and form my personal opinions. 3.10 0.975
BEH15. I can reflect on my own behavior about the social and environmental impacts. 3.31 0.933
BEH16. I am able to communicate relevant environmental-related information to others. 3.18 0.956
BEH17. I am able to convince my family members and friends to undertake environmental actions. 3.14 0.999
BEH18. I know how to use appropriate channels to promote environmental knowledge and
3.09 0.974
environmental friendly policies.
BEH19. I use appropriate channels to appeal environmental complaint issues, to help avoid
2.95 1.036
environmental pollution incident.
BEH20. I will buy polyethylene terephthalate bottled beverages. 3.14 0.895
BEH21. I will bring my own personal water bottle when I go out. 3.28 1.156
BEH22. I will carry reusable utensils (i.e., chopsticks, spoons, etc.). 2.69 1.102
BEH23. I will convey environmental protection information to my family members or friends. 2.90 0.941
BEH24. I have participated in environmental organizations (i.e., green squad, green campus groups, etc.)
2.21 1.020
on campus.
BEH25. I have attended schools’ or extracurricular experiences in environmental activities (i.e., beach
2.30 1.021
cleaning, ecological ponds’ cleaning, and other ecological activities).
BEH26. I have used appropriate channels on environmental complaint issues to help appeal
2.00 1.066
environmental pollution incident (i.e., open burning, free dumping wastes).
BEH27. I have participated in related rallies, marches, or petition activities on environmental policies. 3.14 0.895
R1. I have the responsibility to improve the surrounding environment. 3.74 0.876
R2. It is my duty to prevent environmental pollution incidents. 3.69 0.878
R3. It is my responsibility to solve any environmental problems caused by myself. 3.79 0.876
R4. It is my responsibility to influence the people around me to have better awareness on
3.65 0.882
environmental protection.
Sustainability 2018, 10, 1730 12 of 21
Participation in
Elements Components Mean SD t Value p Value
Clubs/Societies
Yes 3.02 0.674
Knowledge of natural systems 11.249 <0.000 ***
No 2.89 0.736
Cognitive Yes 3.46 1.236
Knowledge of environmental issues 11.217 <0.000 ***
No 3.22 1.335
Yes 3.14 0.852
Knowledge of appropriate action strategies 4.329 <0.000 ***
No 3.07 0.903
Yes 3.88 0.665
Environmental awareness and sensitivity 12.884 <0.000 ***
No 3.73 0.687
Affective Yes 4.11 0.739
Environmental values 13.888 <0.000 ***
No 3.93 0.769
Yes 3.84 0.680
Decision-making attitude on environmental issues 12.141 <0.000 ***
No 3.70 0.688
Yes 3.65 0.686
Intentions to act 11.373 <0.000 ***
No 3.52 0.675
Behavioral Yes 3.27 0.741
Environmental action strategies and skills 9.117 <0.000 ***
No 3.15 0.741
Yes 2.62 0.640
Involvement in responsible environmental behavior 4.602 <0.000 ***
No 2.56 0.672
*** At the significant level of 0.001 (two-tailed).
Figure 1. The AMOS model estimates of parameters in structural model of the cognitive element
(with standardized estimates of coefficients of standardized value, coefficients in t values, coefficients
of determination).
Figure 2. The AMOS model estimates of parameters in structural model of the affective element
(with standardized estimates of coefficients of standardized value, coefficients in t values, coefficients
of determination).
Figure 3. The AMOS model estimates of parameters in structural model of the behavioral element
(with standardized estimates of coefficients of standardized value, coefficients in t values, coefficients
of determination).
between cognitive (r = 0.215) and behavioral elements, and cognitive and affective elements (r = 0.385).
However, the results determined that affective and behavioral elements were highly correlated with r
value of 0.76 [32,41]. The results suggested that the possession of only environmental knowledge and
awareness of environmental issues could not always be successfully transformed into environmental
action [42].
Table 9. AMOS correlations between each element by their estimates for all students.
While some studies [2,30,43] had reported no positive relationship between environmental
awareness and knowledge and pro-environmental behavior, but researchers have continued to be
inspired to investigate the relationships among intrinsic, self-determined, and self-esteemed motivation
induced by personal behavior since personal self-efficacy is directly associated with pro-environmental
behavior [44,45]. In this study, the highest average score observed was in the attitude element (M = 3.73,
SD = 0.631), followed by the behavioral element (M = 3.09, SD = 0.546), with the lowest average value
being the knowledge element (M = 3.08, the average correct response rate = 60.5%). The correlation
between students’ environmental attitudes and environmental behaviors was moderate, and the
value of this correlation was low in the knowledge element. Overall, the undergraduate students’
environmental literacy indicated low environmental knowledge, moderate environmental attitudes,
and low environmental behavior. Further analysis revealed that these students could be broadly
divided into two groups according to the following continuum of the states of environmental literacy:
ecocentric and egocentric [46,47].
Ecocentric engaged students accounted for about 60.2% of the entire sample in this study.
A high ratio of these ecocentric students were female, lived in school dormitories, enjoyed searching
environmental information, and participated in clubs and societies. The results showed that
these students had a moderate level of environmental knowledge (M = 3.35, SD = 0.455), strong
environmental attitudes (M = 4.07, SD = 0.398), and moderate environmental behavior (M = 3.03,
SD = 0.493). In contrast, the remaining 39.8% of the sample that represented the egocentric engaged
students were mainly male who lived with their families or rented apartments, did not enjoy acquiring
environmental information, and limited participation in clubs and societies. Findings suggested
that these students had a low level of environmental knowledge (M = 2.82, SD = 0.731), moderate
environmental attitudes (M = 3.31, SD = 0.581), and a low value of environmental behavior (M = 2.86,
SD = 0.512).
From Table 10 above, the results revealed that there were no significant correlations between
cognitive (r = −0.261) and affective elements, or affective (r = −0.392) and behavioral elements for
egocentric engaged students. However, the findings indicated a moderate relationship between cognitive
(r = −0.552) and behavioral elements whereby egocentric engaged students might have the environmental
knowledge and awareness, but did not necessarily converted into environmental actions.
Table 10. AMOS correlations between each element by their estimates for the bottom 20% of egocentric
engaged students.
Table 11. Cross-tabulation for gender—bottom 20%, 20% to 80%, and top 20%.
Total Clustering
Total
Bottom 20% 20% to 80% Top 20%
Male 3293 7577 2461 13,331
Gender
Female 2062 8432 3071 13,565
Total 5355 16,009 5532 26,896
4.3. Relationship between Clubs and Societies Participation and Environmental Literacy
Undergraduate students who participated in clubs and societies at universities/colleges generally
performed better in their environmental literacy than those who did not. This could be explained with
the fact that being involved in activities through clubs and societies provide more opportunities for
creative thinking, problem solving, leadership, and prosocial behavior than regular courses do [50].
In addition, participants who had pro-environmental experience in these activities gained considerably
self-respect, self-esteem, and self-confidence [51–53]. Majority (91%) of the undergraduate students in
the bottom 20% who had poor environmental literacy did not participate in clubs and societies while
the remaining 9% had participation. Table 12 below provides a summary of the findings.
Table 12. Cross-tabulation for participation in clubs and societies—bottom 20%, 20% to 80%, and top 20%.
Total Clustering
Total
Bottom 20% 20% to 80% Top 20%
Participate in clubs Yes 471 2103 1039 3613
and societies No 4892 13,949 4526 23,367
Total 5363 16,052 5565 26,980
medium to disseminate environmental knowledge. With the increasing ease of access and vast amount
of information available via the internet, online learning has become an important environmental
knowledge source and this was supported by approximately 48.4% of the undergraduate students
who participated in this study. Given this, a focus on utilizing the online channel as a source of
environmental knowledge is deemed to be critical since students nowadays are more internet savvy
than ever.
components and corresponding items were more closely related to local issues and dimensions in the
Taiwanese context. However, it is recommended that the results in this study be used as a benchmark
for comparison with other similar studies conducted in other countries, especially those that are
developing and characterized by rapid industrialization and urbanization.
Next, prior studies investigated the predictions about pro-environmental behavior based on moral
or ethical elements have found that personal norms played a critical role. However, since this study
did not explore the effects of the moral and ethical elements, therefore, conclusions could be provided
in this aspect. Thus, it is recommended that further detailed studies about the impact of moral and/or
ethical norms on environmental literacy could be conducted in Taiwan in the future, in order to gain
further insights and understanding in this field.
Lastly, the low composite reliability of the cognitive element was an indication that some items
were not necessarily representative but because of the limited number of items, therefore the removal
of some odd items was deemed unsuitable. Thus, it is recommended to increase the items (in interval
measurement Likert-type scale format) in the questionnaire to improve the reliability.
Author Contributions: S.-W.L., W.-T.F., S.-C.Y., S.-Y.L. and H.-M.T. conceived and designed the experiments;
S.-W.L. performed the experiments; S.-W.L. and W.-T.F. and E.N. analyzed the data; S.-W.L., W.-T.F., S.-C.Y., S.-Y.L.,
H.-M.T. and J.-Y.C. contributed reagents/materials/analysis tools; W.-T.F., J.-Y.C. and E.N. wrote the paper.
Acknowledgments: This research was supported by the Environmental Protection Administration, Taiwan, R.O.C.
under Grant number EPA-100-EA11-03-A264; and the Ministry of Science and Technology, Taiwan, R.O.C. under
Grant number MOST-105-2511-S-003-021-MY3 to W.-T.F. Our gratitude also goes to the financial supports from
National Taiwan Normal University.
Conflicts of Interest: The authors declare no conflict of interest.
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