PPT DNA Recombineering Technology

Group 3
DNA
Recombineering
Technology
Lectures: Dr. Suci Fajrina, M.Pd.
Group 3
1. Bunga Fadelia (22031125)
2. Damairis (22031165)
3. Octavia Putri Azzahrianto (22031095)
4. Rifaa Salsabilla (22031103)
5. Septi Wulandari (22031109)
1.
Definition of DNA
Recombination
Technology
What is DNA
Recombinant Technology?
DNA recombinant technology is a molecular
biology technique that enables genetic
manipulation by combining DNA from two or
more different sources into a new DNA
molecule. This technology is often used to
create organisms with specific traits,
produce important proteins such as insulin,
or conduct genetic research.
Applications of DNA
Recombinant Technology
Scientific
Health and Agriculture Industry
Research
Medicine Example: Example:
Example:
Example: Nutrient- Production of
Study of gene
Recombinant enriched crops enzymes for
and protein
vaccines. food
function
Advantages and
Weaknesses
Advantages: Weakness:
·Allows genetic manipulation with ·Risks to the environment, such as
high precision. uncontrolled release of transgenic
·Can be used to correct health and organisms.
environmental problems. ·Food safety issues in GMO
·Accelerates the development of products.
biotechnology products. ·Ethical considerations in genetic
manipulation, especially in humans.
2.
Principle of DNA
Recombinant
What is DNA
Recombination?
Establishment of new genes through the process of insertion

of foreign genes through laboratory techniques.
Stages in carrying out DNA
recombination
Recombinant DNA refers to the combining of
genetic material from two different sources
to create a new organism or to produce a
specific protein. The main purpose of
genetic recombination is to produce more
evolved and adapted organisms and develop
biotechnology strategies such as
recombinant protein production.
Stages in carrying out DNA
recombination
1 DNA isolation:
* The DNA to be recombined is isolated from the source organism.
* Isolation methods may vary depending on the type of organism and the type of DNA to
be isolated.
2. DNA cutting:
* Restriction enzymes are used to cut DNA at specific base sequences, resulting in DNA
fragments with sticky ends or blunt ends.
* Sticky ends are easier to connect as they have complementary base sequences.
3. Vector Selection:
* Vectors are DNA molecules that are used as vehicles to carry foreign DNA fragments into
the host cell.
* Commonly used vectors are bacterial or viral plasmids.
Stages in carrying out
DNA recombination
4. Vector Cutting:
* Vectors are also cut with the same restriction enzymes used to cut
the target DNA, resulting in compatible ends.
5 Joining of DNA Fragments (Ligation):
* The target DNA fragments and the cut vector are joined using ligase
enzymes.
* The ligase enzyme will connect the sticky or blunt ends of the two
DNA fragments, forming a recombinant DNA molecule.
Principle of Isolating
DNA
Separates DNA from other cell components
Materials:
Damages cells by Damages the cell Damages Separates DNA Clears

binding to membrane proteins in cells bands from polysaccharides
magnesium ions proteins and that may be there
debris
Benefits of doing
DNA Recombination
Production of therapeutic proteins.
Development of genetically modified organisms
Creation of targeted gene therapies.
Enhanced agricultural crops.
Production of biofuels.
Study of gene function and expression.
Creation of vaccines.
Development of diagnostic tools.
Bioremediation and environmental cleanup.
Advancement of personalized medicine.
3.
Cloning Gen
WHAT IS GENE CLONING Penjelasan DEFINITION OBJECTIVE
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increase the
productivity of
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Basic Principles of Gene Cloning
Gene Cloning Application
Genetic Vaccine
Recombinant Genetic Research: Production:
Protein Engineering: Understanding Example:
Production: Pest resistant the mechanisms Recombinant-
Example: Insulin plants (BT of genetic based hepatitis B
for diabetes. Corn). disease. vaccine.
Advantages and
Weaknesses
Profit: Weakness:
High efficiency in producing Biotechnology ethics and safety.
specific proteins. Possible side effects of genetic
Supports genetic and medical engineering.
research. Regulations and policies related to
Wide application in agriculture, genetic engineering.
pharmaceuticals and environment.
Step of Cloning Gen
isolation DNA DNA Ligation Selection and Identification
DNA Cutting Transformation

Benefits of gene
cloning
Genetic Research
Recombinant Protein Production
Gene Therapy
Plant and Animal Breeding
Disease Studies
New Drug Discovery
Bioremediation
Storage and Development of Genetic Data
Gene Cloning
Controversy
Human Cloning
Animal Welfare
Genetic Diversity
Health Security
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Group 3

Establishment of new genes through the process of insertion

Damages cells by Damages the cell Damages Separates DNA Clears

DNA Cutting Transformation

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