Blood Grouping Pract 4
Blood Grouping Pract 4
Blood Grouping Pract 4
FIRST SEMESTER
PRACTICAL Three (4)
COURSE TITLE: HAEMAOLOGY PRACTICAL
COURSE CODE: MLS 3307
Blood Group Serology Practical
K - + - A blood group
L + - - B blood group
M - - - AB blood group
N + + - O blood group
Comment:
Serum sample K was from blood group A individual
Serum sample L was from blood group B individual
Serum sample M was from blood group AB individual
Serum sample N was from blood group O individual
Title: ABO and Rhesus Cell grouping
Aim: To determine the specific antigen present in a given blood sample
Method: Tube method
The tube technique is employed for all blood group tests. It is recommended for
confirmation of slide/tile test results when it is doubtful and whenever there is
enough time.
Materials/reagents: blood samples, test tube, racks, applicator sticks, water bath
Anti sera A, B, AB and D
Procedure:
Prepare 5% cell suspension for each test and control sample
Arrange the required number of precipitin tubes in to a rack
Place one volume of unknown cells/serum of each test and control sample
Add equal volume of known serum (Antisera)/cells (5% suspension) to the
appropriate tube
Mix the tubes gently and allow to stands at room temperature for 1½ hours
Incubate tubes containing ant-D at 37OC for 1½ hours
Add one volume of bovine albumin to tube containing ant-D after 1½ hours
and re-incubate for further 30 minutes
Read result macroscopically by gently tapping the tube and microscopically
if no observable reaction was observe macroscopically
Protocol: