31-Aug-2021-08_10pm-37961-291791
31-Aug-2021-08_10pm-37961-291791
31-Aug-2021-08_10pm-37961-291791
Comments
***** KINDLY NOTE: THE HBV VIRAL LOAD CAN BE EXPRESSED AS LOG 3.63 AND THE CALCULATED VALUE
IS 35393 CPS/ML (CONVERSION FACTOR: 1 IU/ML= 8.21 CPS/ML).
Interpretation(s)
HBV VIRAL LOAD BY REAL TIME PCR-
Clinical Utility: The viral load provides the direct and reliable estimate of the level of HBV replication. Quantitation of HBV DNA level is important as it serves to be a
prognostic marker of HBV infection. It is used for establishing baseline levels in patients before initiation of the therapy and for monitoring therapeutic response and disease
progression. A sudden rise in the viral load may indicate emergence of resistant strains during the therapy.
Interpretation: HBV viral load is expressed as IU/ml. For conversion to WHO International Units (IU): 1 IU corresponds to approximately 8.21 copies/ml. The lower limit of
detection of this assay is 32 IU/mL. Values below 32 IU/ml does not exclude the possibility of an infection. It may reflect a viral load below the detection limit of the assay.
An increase or decrease of more than threefold may be considered clinically significant. Follow up viral load values below the detectable limit may indicate resolution of the
infection after therapy. Reappearance or increasing viral load may indicate relapse or resistance to the therapy. All viral load results should be interpreted in conjunction
with the clinical history, clinical status of the patient and other diagnostic parameters.
Recommendations: Viral load is a monitoring test and hence should not be used for screening or diagnostic purpose. Wide variations in viral load have been observed due to
following reasons:
a) Use of different technologies/ platforms for follow up testing. Hence, it is recommended to monitor patients using same technology.
b) Non adherence to specimen collection protocol. Hence, it is recommended to immediately freeze the serum/EDTA plasma after collection and separation.
Limitations: PCR is a highly sensitive technique common reasons for paradoxical results are contamination during specimen collection, selection of inappropriate specimens
and inherent PCR inhibitors in the specimen.
References
1. Hepatology (1989) 10: 198202
2. New England Journal of Medicine (1990) 323:295301.
3. Hepatology (1997) 25: 241244.
4. Antiviral Res (1997) 35: 65 82.
5. WHO: Blood Safety & Clinical Technology (2002) 19.
Note: The performance of this assay has been evaluated at SRL Limited.
**End Of Report**
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