GCU External PG Microbial Metabolism 2023 2

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EXTERNAL BOS

Date 28/08/2021 Timing 2:30 PM 4:00 PM


Place Garden City university Venue Online MS Teams
Programme Title MSc Microbiology Programme Abbreviation MSMB
Programme 2 Years Number of Semesters 4
Duration
School Sciences Department Life Sciences
School Code 02 Programme Code 02BMSMB
Number Number
Programme 2017 Expected Programme 2025
Commencement Revision Year
Year
Total Number of 28 Total Number of Core 24
Courses Courses
Total Number of NA Total Number of SEC NA
AECC
Total Number of NA Total Number of GE NA
DSE
Total Number of 1 Total Number of Projects 2
Internships
Total Core 82 Total AECC Credit NA
Course Credit
Total DSE Credit NA Total SEC Credit NA
Total GE Credit NA Total Credit for 16
Internships
Total Credit for Minor Project:04 Total Credit for the 105
Project Major Project: 08 Programme
S.No External BOS Member Signature & Date
Name
1. Dr. Bharathi S
2.
Created By Ms. Florence Shoba
Reviewed By Dr. Sheeja Rajesh
Approved By Dr. Preethi Rajesh
Note:
1. This form shall be typed and printed. Only the signature and date should be handwritten.
2. The format should not be altered. Any alteration in the format will not be accepted.
3. Times New Roman font, size 11 pt., and unbold text should be used.

 AECC : Ability Enhancement Compulsory Course


 SEC : Skill Enhancement Course
 DSE : Discipline Specific Elective
 GE : Generic Elective
EXTERNAL BOS

COURSE SYLLABUS CREATION/REVISION DOCUMENTATION


( to be filled for each course)
Semester I Number of times the 2
course has been revised
Course Microbial Metabolism Course Commencement 2017
Title year
Course 02BMSMB23163 Current Revising year 2023
Code
Credits 4 Effective from the 2023
academic year
Course Ms. Florence Shoba Course Revision Dr. Sheeja Rajesh
Reviser Reviewer Name
Name
Unit I Proposed Revision Justification
Unit – 1 Microbial Nutrition
Change in the Title To make the title more
1.1. Microbial nutrients:
specific to the topics
 Carbon sources, Energy sources & covered in the
syllabus
Hydrogen/ electron sources

 Nutritional types of bacteria based on To make the subunits


Unit is revised by more specific and to
the nutritional requirements and
renaming the subunits keep up to the
sources of nutrients. and placing them in an connectivity and flow
altered order. of knowledge.
(K, L, A, Ap)
1.2. Nutritional uptake:
Bacterial nutrition is
Bacterial nutrition & removed from
Types & mechanisms of nutritional
Nutritional types of Bacteriology &
uptake by the microbial cell:
bacteria are added Virology course and
added here as it is part
 Passive transport
of Microbial
 Facilitated diffusion
metabolism
 Active transport
 Group translocation
 Iron uptake & siderophores
(K, L, A, Ap)

1.3. Stress responses in microbes:

Responses to osmotic stress,


oxidative stress, thermal stress and
heat shock, response to starvation
EXTERNAL BOS

stress.

Secondary metabolites & biofilm


formation.

(K, L, A)
1.4. Signal transduction in
Prokaryotes - Chemotaxis, Quorum
sensing, sporulation inducing
signals and events in sporulation.
Bioluminescence & its application
(A, Ap)
Unit II Unit 2: Microbial Metabolism Unit is revised by To make the subunits
rearranging the more specific and to
2.1. Principles and Concepts in subunits and placing keep up to the
Microbial Metabolism them in an altered connectivity and flow
 The laws of thermodynamics & Free order. of knowledge.
energy and reactions
 Structure & role of ATP in
metabolism, Enzymes and its These topics are
 Oxidation- reduction reactions action & inhibition more
 Electron transport chain electron are removed from appropriate here
carriers. Unit 4 of the same with the
 Metabolic Diversity in micro-
course and added into principles and
organisms.
unit 2 concepts of
(K, L, A, Ap) Microbial
metabolism
2.2. Mechanism of enzyme action and
its inhibition
 Basic structure and properties of
enzymes.
 Enzyme kinetics: Miachelis Menten
Equation, Vmax, Km.
 Mechanism of enzyme action: lock
and key and induced fit hypothesis.
Inhibition of enzymes – Types of
inhibition.
 Enzyme Regulation
EXTERNAL BOS
(L, A, Ap)

2.3. Bacterial Respiration and ATP


Synthesis
 Aerobic and Anaerobic
respiration.

 Substrate level phosphorylation,


Oxidative phosphorylation.
Generation of ATP in
chemolithotrophs - hydrogen-
oxidizing bacteria, nitrifying
bacteria, and sulfur-oxidizing
bacteria.

 Assimilation of Inorganic
phosphorus, sulphur and Nitrogen.
Ammonifying bacteria & Iron
oxidizing bacteria

(K, L, A, Ap)
2.4 Pathways in Carbohydrate
Metabolism
 Glycolysis, TCA cycle, Glyoxylate
cycle. Pentose phosphate pathway,
Gluconeogenesis, Entner – Doudoroff
pathway, Phosphoketolase pathway.

 Fermentation pathways: Homo


and Heterolactic fermentation - lactic
acid fermentation, acetic acid, butyric
acid, mixed acid and propionic acid
fermentation.

(K, L, A)

Unit III 3. Microbial Metabolism Unit is revised by To make the subunits


rearranging the more specific and to
3.1 Lipids metabolism: subunits and placing keep up to the
them in an altered connectivity and flow
EXTERNAL BOS
 Biosynthesis of lipids order. of knowledge.

 Oxidation of fatty acids (β


oxidation)

(K, L, A)
3.2. Nucleic acids:
 Biosynthesis of Purine and pyrimidine,
denovo and salvage pathway.

(K, L, A)
3.3. Amino acids metabolism:
 General reaction in catabolism of
amino acids- transamination,
Deamination, Decarboxylation.

 Basic pathways of amino acid


synthesis- aliphatic and aromatic
aminoacids.

(K, A, Ap)
Unit IV Unit 4: Metabolic Regulation and Unit is revised by To make the subunits
Bacterial Photosynthesis rearranging the more specific and to
subunits and placing keep up to the
4.1. Bacterial photosynthesis: them in an altered connectivity and flow
 Light & dark reactions, oxygenic and order. of knowledge.
anoxygenic photosynthesis. Carbon
dioxide fixation, Reduction phase,
Regeneration Phase

(K, L, A)
4.2 Metabolomics:
 Concepts, Levels of metabolite
analysis, sample selection and
handling. over view of different
methods used for analysis of
metabolites and metabolic
engineering.
EXTERNAL BOS
(K, L, A)

REVISIONS BEYOND SYLLABUS

S. No Amendment Title Amendment Justification


Description
1. NA NA NA
2. NA NA NA
3. NA NA NA
4. NA NA NA

EXTERNAL BOS MEETING MINIUTES

Few concepts were added as per the suggestions by External and Internal BOS members

Whether the internal BOS members reviewed the proposed Yes


revisions?

Whether the internal BOS members approved the proposed Yes


revisions?

S.No Internal BOS Member Name Signature


1. Dr. Sheeja Rajesh
2. Dr. Prema Kulkarni
3. Dr. Preethi Rajesh
4.
5.
6.

Note:
1. This form shall be typed and printed. Only the signature and date should be handwritten.
2. The format should not be altered. Any alteration in the format will not be accepted.
EXTERNAL BOS
3. Times New Roman font, size 11 pt., and unbold text should be used.

BOS assessment parameters Comments of the BOS Member

Changes to be made for unit I


Review

Done

Comparison
New concepts are added in unit I

What's New

Done
Depth Analysis

Included
Research Component

Included
Technical Skills
Yes

Industry Relevance

Yes
GCU Standards
Yes

Joy of Learning Component


TEACHING PLAN

COURSE: FUNDAMENTALS OF BACTERIOLOGY AND VIROLOGY

CODE: 02BMSMB23163 CREDITS: 2

Week Topic Hours Teaching Methods


EXTERNAL BOS
1-15  Study of stress responses in bacteria 120 1. Composition and preparation
of buffers, reagents & stock
 Estimation of protein from microbes 4 Hours per solution
week 2. Protocol Demonstration
& its estimation by Lowry’s method
3. Experimental discussion
 Estimation of protein by Bradford 4. Individual hands on
method experiments
5. Drawing of figures and graphs
 Estimation of reducing sugars by 6. Derivation of equations
DNS method 7. Group assignments
8. E-content related to
 Estimation of amylase activity Km & experiments
Vmax of amylase 9. Discussions
10.Industrial visit related to the
 Effect of pH on amylase activity experiments
 Effect of temperature on amylase
activity
Fractionation of microbial lipids
by Column chromatography
16 END SEMESTER EXAMINATION

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