3Biochemical Test of Bacteria Notes - Microbe Notes

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Biochemical Test Topics / Categories


Agricultural Microbiology (12)
Biochemical tests are the tests that are performed on different bacteria for their
Bacteriology (123)
identification on the basis of their biochemical activities towards different biochemical
compounds. Basic Microbiology (60)

Biochemical Test (114)


Biochemical tests are one of the traditional methods for the identification of
microorganisms, usually performed with phenotypic identification. Biochemistry (155)

For many years these methods were employed extensively, and they continue to be used Bioinformatics (21)

nowadays, especially in some laboratory routines where a particular type of Biology (183)
microorganism has to be identified rapidly. Biotechnology (28)
The ability of microorganisms to utilize certain biomolecules, resulting in useful organic Book Reviews (1)
compounds for themselves forms the basis of various biochemical tests. Cell Biology (94)
Biochemical tests are of different types, where the identification or distinction between
Culture Media (66)
different microorganisms is made on various bases.
Difference Between (89)
One of the traditional methods commonly used is a simple visual detection of the growth
Diseases (29)
of the organism in the presence of essential nutrients by increased turbidity in the liquid
Environmental Microbiology (6)
medium.
In other tests, however, the results are based on the change in color of the medium as a Epidemiology (23)

result of the change in the pH of the medium. Food Microbiology (49)

Microorganisms can be classified into different groups on the basis of their reaction to Genetics (78)

such tests. Some tests even allow the distinction of microorganisms to the species level. Human Anatomy and Physiology (70)
Biochemical tests are thus, essential as they are inexpensive and relatively simple to Immunology (115)
perform. Instrumentation (63)
The physiology of bacteria and other microorganisms differs from one another, which
Journals (1)
allows for the differentiation of such microorganisms.
Microscopy (28)
Biochemical tests, however, have some disadvantages. Despite being inexpensive and
Molecular Biology (66)
allowing both quantitative and qualitative information about the diversity of
Mycology (33)
microorganisms present in a sample, these methods are laborious and time-consuming,
Parasitology (25)
and results are only observed after several days.
In some cases, false positives are obtained, especially when considering similar microbial Pharmacology (14)

species. Phycology (2)

Protocols (9)

Research Methodology (19)


Nitrate Reduction Test- Principle, Procedure, Types, Results, Uses
Staining (29)
January 2, 2024 by Prashant Dahal
Syllabus (18)

In the anaerobic respiration process, bacteria derive Universities (4)

their required oxygen from nitrate (NO3–) or can use Virology (50)
NO3– as a terminal electron acceptor. In either of
these processes, nitrate is first … Read more

Sulfur Reduction Test: Principle, Procedure, Results, Uses


October 6, 2023 by Prashant Dahal

Elemental sulfur and sulfur-containing compounds –


both organic and inorganic compounds – can be
metabolically reduced by some microorganisms to
obtain metabolic energy. Such microorganisms are
LEAM Solution Fluorescence Microscope
called sulfur-reducing microorganisms. Several …
Read more

TSIA Test: Principle, Media, Procedure, Results, Uses


October 5, 2023 by Prashant Dahal

Hyaline Casts: Structure, Types, Process,


When it comes to the biochemical activities and
Importance
enzyme systems, each bacterial genus has its unique
Secondary Succession: Stages, Examples,
characteristics. These unique characteristics are the
Importance
basis for differentiating and identifying bacteria
Primary Succession: Stages, Examples,
based on … Read more
Importance

Mucous Membrane: Structure, Location,


VP Test: Principle, Reagents, Procedure, Results, Uses Functions, Disorders

October 4, 2023 by Prashant Dahal Secondary Consumers: Types, Food Chain,


Examples, Roles
Although most bacteria are able to ferment
(metabolize) glucose, the end product of glucose
metabolism by different bacteria might not always be
the same. Following glycolysis, some follow the
mixed … Read more

Satellitism Test: Principle, Media, Procedure, Results, Uses


October 3, 2023 by Prashant Dahal

Haemophilus spp. requires either or both of the X-


factor (hemin or protoporphyrin IX) and V-factor
(nicotinamide adenine dinucleotide (NAD)) for their
growth and survival. Haemophilus spp. can be
distinguished down … Read more

Spot Indole Test: Principle, Procedure, Results, Uses


October 1, 2023 by Prashant Dahal

Spot indole test method is a rapid indole detection


method where bacteria cultivated in the tryptophan-
rich medium are directly exposed to an Indole
Reagent for detection of indole production. The …
Read more

Starch Hydrolysis Test: Principle, Procedure, Results, Uses


August 1, 2023 by Sagar Aryal

Starch is the reserved food material of most plants


and is one of the abundant carbohydrates in nature.
Starch is a polysaccharide made of two glucose
polymers –amylose and amylopectin. … Read more

X and V Factor Test: Principle, Procedure, Results


July 15, 2023 by Sagar Aryal

Haemophilus spp. is a genus of Gram-negative,


aerobic or facultatively anaerobic, pleomorphic
(mostly coccobacilli), Gammaproteobacteria of the
phylum Pseudomonadota and family Pasteurellaceae.
Several species of the genus Haemophilus are
pathogenic … Read more

Beta (β) Lactamase Test: Principle, Types, Procedure, Results


May 30, 2023 by Babita Sharma

Beta (β) Lactamase Test is used to detect beta-


lactamase enzymes produced by different bacteria.
Objectives To detect the enzyme beta-lactamase by
chromogenic, iodometric, and acidometric tests,
which confers beta-lactam resistance … Read more

LAP Test- Principle, Procedure, Results, Uses


April 16, 2023 by Prashant Dahal

LAP test is used for the differentiation and


identification of catalase-negative Gram-positive
cocci bacteria. Specifically, it is used to distinguish
Streptococcus, Enterococcus, Pediococcus, and
Lactococcus species from Aerococcus and
Leuconostoc … Read more

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