Anaerobic System

COMPLETE ANAEROBIC SYSTEM

For fast results and accurate diagnosis from a completely centralized oxygen-free work chamber, choose Thermo Forma Anaerobic System Model 1025 (1029). Safely inoculate, incubate, examine, and subculture even the most fastidious organisms. This complete anaerobic system provides the culture, work, and storage space for all of your needs, and is expandable to accommodate the requirements of growing labs.

Model 1025 (1029) Anaerobic Systema centralized oxygen-free work chambershown with optional bench and vacuum pump

FEATURES AND BENEFITS

Condensate-free stainless steel work chamber includes two shelves that are adjustable on 1.0" (2.5cm) centers, four electrical receptacles, two 15 Watt fluorescent lights, and a warm air jacketed incubator. Built-in 2.72 cu. ft. (77.0 liters) desiccation-free incubator provides a stable temperature that can be fixed at 35C (95F) or variably set from 4C above ambient to 70C (158F). Centrally located oval glove ports allow free access to all areas of the work chamber. Soft, clear vinyl front slopes gently for maximum visibility. Optional polycarbonate hard front is available. Optional single item entry system in the vinyl front allows rapid movement in and out of the chamber for small items. Control panel includes automatic and manual control of the interchange autosequence, indicator lights to monitor function status, and fixed and variable temperature control to regulate the incubator temperature.

Adjustable overtemperature safety thermostat is standard. Audible alarm warns you of overtemperature conditions. Easily accessible palladium catalyst and desiccant wafers work together to maintain strict anaerobiosis (less than 10 ppm O2). Charcoal wafer removes hydrogen sulfide (H2S) and extends the life of the palladium wafer. Auto-sequence cycle completely automates oxygen reduction of the interchange atmosphere. All utility connections are on the recessed panel at the rear of the cabinet. Unit requires only anaerobic grade gas mixture and nitrogen. Thermo Forma Anaerobic Systems include a full one year parts and labor warranty.
Built-in incubator for incubation capabilities within the anaerobic environment

ACHIEVING LESS THAN 1% O2 CONDITIONS

Stringent anaerobiosis is maintained inside the cabinet with a palladium catalyst wafer and a desiccant wafer. Palladium pellets and desiccant crystals, sealed in individual screen wraps, minimize sifting. The reusable palladium catalyst and desiccant wafers can be regenerated periodically in a standard laboratory oven to minimize operating costs.

H2S
Charcoal Filter Removes hydrogen sulfide and extends the life of the palladium wafer. Longevity: Three months Regeneration: Cannot be regenerated

trace

O2

2H2

Palladium Catalyst Wafer Bonds trace oxygen to hydrogen in the anaerobic gas mixture, forming water vapor. Longevity: Two years (at 10% H2) Regeneration: Once per week; bake two hours at 160C (320F)

2H2O
absorbed
Desiccant Wafer Absorbs the water vapor. Longevity: Two years Regeneration: Two times per week; bake two hours at 160C (320F)

All three wafers are inserted into the airflow system.

The door is closed, and the system continuously circulates the chamber atmosphere through the wafer system.

CONTROL PANEL
Auto-Sequence Cycle Includes on/off switches for the gas, lights, and catalyst. Also includes manual/auto switches for the vacuum, nitrogen purge, and equalization steps of the cycle. Lights indicate that a function has been activated. Interchange Indicates the interchange gas pressure.

Incubator On/Off Switch

Incubator Power Green light indicates that the incubator is on. Heat Indicates that the incubator is heating.

Manual Chamber Fill Is used to increase the pressure in the chamber. Chamber Relief Prevents the chamber from overpressurizing, while keeping out ambient air. Interchange Status Includes the Start Cycle button and lights that indicate the cycle and door status.

Incubator Temperature Control Can be set to a constant 35C (95F) or variably set from 4C above ambient to 70C (158F).

AUTOMATIC INTERCHANGE
The automatic interchange allows you to transfer specimens to the work chamber without exposing the specimens or work chamber to lab air. Outer door opens to the front so the anaerobic system can be located against a wall. Lights on the control panel indicate chamber functions, including all phases of the automatic interchange. Lights also denote interchange status by signaling when it is safe to transfer specimens without contaminating the anaerobic chamber.

Interchange compartment features a sliding floor that allows materials to be easily introduced into the working chamber. Interchange holds stacked culture dishes, glass jars, test tube racks, and other items used in the work chamber. The oversized compartment accommodates the largest GasPak jar.

Inner door may be opened and materials moved into the work chamber when the status light on the control panel indicates an anaerobic atmosphere. GasPak is a trademark of Becton, Dickinson and Co.

AUTO-SEQUENCE CYCLE
The auto-sequence cyclea completely automatic oxygen reduction of the interchange atmosphereis activated by a single, push-button control. Merely press the Start Cycle button after all materials to be transferred are loaded in the interchange, and the outer door is securely clamped. The auto-sequence cycle is preset for three evacuations, two nitrogen purges, and one pressure equalization of anaerobic gas from the work chamber. When the auto-sequence is completed, the atmosphere in the interchange is anaerobic, as indicated by the Sequence Complete status light on the control panel. You may then open the inner door to move materials from the interchange to the work chamber.

Step 1 Interchange Vacuum Function Time: 18 seconds Elapsed Time: 18 seconds Inner and outer interchange doors are sealed. Vacuum pump pulls down to 20 in./Hg. Interchange is 95% oxygen free.

Step 2 Interchange N2 Purge Function Time: 1 minute, 4 seconds Elapsed Time: 1 minute, 22 seconds Interchange fills with pure nitrogen gas until atmospheric pressure is reached.

Step 3 Interchange Vacuum Function Time: 18 seconds Elapsed Time: 1 minute, 40 seconds Second vacuum pulls down to 20 in./Hg to evacuate nitrogen and trace oxygen to a 96% oxygen-free chamber.

Step 4 Interchange N2 Purge Function Time: 1 minute, 4 seconds Elapsed Time: 2 minutes, 44 seconds Interchange fills with pure nitrogen gas until atmospheric pressure is reached.

Step 5 Interchange Vacuum Function Time: 17 seconds Elapsed Time: 3 minutes, 1 second Final vacuum pulls down to 20 in./Hg to evacuate nitrogen and trace oxygen to a 98% oxygen-free chamber.

Step 6 Interchange Equalization Function Time: 1 minute, 31 seconds Elapsed Time: 4 minutes, 32 seconds Interchange is purged with anaerobic gas from the work chamber to equalize both pressure and atmosphere. Inner door may be opened after the anaerobic status light (Sequence Complete) is on. Palladium catalyst wafer removes trace oxygen to ensure less than 1% O2 conditions.

SPECIFICATIONS
Incubator Temperature Control . . . . . . . . . . . . . . . . . . 0.3C Range . . . . . . . . . . . . . . . . . . . 35C (95F) fixed; variable 4C above ambient to 70C (158F) Thermostat/Alarm . . . . . . . . . . Adjustable overtemperature safety thermostat and audible alarm Utility Connections Mixed Gas . . . . . . . . . . . . . . . 1/4" compression fitting Nitrogen Gas. . . . . . . . . . . . . . 1/4" compression fitting Vacuum Pump. . . . . . . . . . . . . 1/2" MPT Gas Supply Anaerobic Gas . . . . . . . . . . . . High purity grade, 85% N2 /10% H2 /5% CO2 (or mixture containing 5-7% H2 Nitrogen Gas. . . . . . . . . . . . . . High purity grade Vacuum Pump Optional Vacuum Pump. . . . . . Stock No. 901024 115V, 60 Hz Stock No. 901025 230V, 50 Hz 7.2 cfm pulls down interchange to 18 in. Hg in 15 seconds. In-house vacuum systems must meet 4 cfm capacity to 24 in. Hg minimum requirements. (203.7 cfm pulls down interchange to 0.6 bar in 15 seconds. In-house vacuum systems must meet 113.2 cfm capacity to 0.8 bar minimum requirements.) Optional Foot Switch . . . . . . . Stock No. 255023 plugs into electrical receptacle included on the unit Incubator Capacity Plate Capacity . . . . . . . . . . . . . 425 (100 x 15mm plates) Dimensions Anaerobic System Exterior . . . 60.6"W x 30.0"H x 31.0"F-B (153.9cm x 76.2cm x 78.7cm) Incubator Interior . . . . . . . . . . 17.5"W x 22.0"H x 12.0"F-B (44.5cm x 55.9cm x 30.5cm) Interchange Opening . . . . . . . . 10.6"W x 11.4"H (26.9cm x 29.0cm) Interchange Depth. . . . . . . . . . 11.0"F-B (27.9cm) Work Surface . . . . . . . . . . . . . 42.5"W x 15.0"F-B (108.0cm x 38.1cm) Shelves Work Area . . . . . . . . . . . . . . . Two adjustable stainless steel shelves on 1.0" (2.5cm) centers, 18.8" x 13.0" (47.8cm x 33.0cm) Incubator . . . . . . . . . . . . . . . . Two adjustable stainless steel shelves on 0.5" (1.3cm) centers, 7.5" x 12.0" (19.1cm x 30.5cm) Construction Interchange . . . . . . . . . . . . . . 11 gauge 2B stainless steel Incubator . . . . . . . . . . . . . . . . 20 gauge 2B stainless steel Exterior Work Chamber . . . . . 16 gauge 2B stainless steel, powder coated exterior Electrical 1025 . . . . . . . . . . . . . . . . . . . 120V, 60 Hz, 14.6 FLA, includes outlets and vacuum pump (Operating range 108-125V) 1029 . . . . . . . . . . . . . . . . . . . 220V, 50 Hz, 7.0 FLA, includes outlets and vacuum pump (Operating range 216-250V) Plug . . . . . . . . . . . . . . . . . . . . NEMA 5-15P (1025), CEE 7/7 (1029) Convenience Receptacles . . . . 4 receptacles, 1 foot operated; 1025: 5 Amps; 1029: 2 Amps Weight 1025 Net . . . . . . . . . . . . . . . . . . . . 290 lbs. (131.5 kg) Shipping (Motor) . . . . . . . . . . 390 lbs. (176.9 kg) 1029 290 lbs. (131.5 kg) 525 lbs. (238.1 kg)

Continuing research and improvements may result in specification changes at any time.

ACCESSORIES
NUMBER 1018 1016 1031 1037 188032 188035 188022 188027 188012 190011 931024 191022 191024 191025 961024 255023 901024 901025 921025 921036 921031 921032 921033 921034 188014 188015 188016 961027 961028 5599000 5599017 5699997 DESCRIPTION Add-On Glove Cabinet for Model 1025, Customer Installed Add-On Glove Cabinet for Model 1029, Customer Installed Add-On Glove Cabinet with Incubator for Model 1025, Customer Installed Add-On Glove Cabinet with Incubator for Model 1029, Customer Installed All Add-On Glove Cabinets are 43.0"W x 30.0"H x 31.0"F-B (109.2cm x 76.2cm x 78.7cm) Hard Front Polycarbonate Panel, Customer Installed Hard Front Polycarbonate Panel (in lieu of vinyl front), Factory Installed Replacement Vinyl Front Panel, Customer Installed Vinyl Front Ocular Pouch (adapts the anaerobic system to your microscope), Customer Installed Single Item Entry Port, Customer Installed Auxiliary Desiccant/Palladium Dryer for Model 1029, Customer Installed Auxiliary Desiccant/Palladium Dryer for Model 1025, Customer Installed Activated Charcoal Filter, Customer Installed Palladium Catalyst Wafer, Customer Installed Desiccant Wafer, Customer Installed Wafer Storage Container Foot Switch (plugs in to electrical receptacle included on the unit), Customer Installed Vacuum Pump for Model 1025, Customer Installed Vacuum Pump for Model 1029, Customer Installed Disposable Waste Bags, 6.0"W x 12.0"H x 6.0"F-B (15.2cm x 30.5cm x 15.2cm), 100 per package Absorbent Work Mats, 18.0" x 20.0" (45.7cm x 50.8cm), 350 per case Size 8 Rubber Gloves (one dozen) Size 9 Rubber Gloves (one dozen) Size 10 Rubber Gloves (one dozen) Size 11 Rubber Gloves (one dozen) Size 8 Neoprene Sleeve/Glove for Hard Front Size 9 Neoprene Sleeve/Glove for Hard Front Size 10 Neoprene Sleeve/Glove for Hard Front Two-Stage Gas Regulator for N2 Two-Stage Gas Regulator for Anaerobic Gas Anaerobic Bench, 66.0"W x 37.0"H x 30.0"F-B (167.6cm x 94.0cm x 76.2cm) Add-On Anaerobic Bench, 44.0"W x 37.0"H x 30.0"F-B (111.8cm x 94.0cm x 76.2cm) Caster with Brake for Anaerobic Bench (order 4)

Vacuum pump

Auxiliary desiccant/palladium dryer

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Rev. 0, 7/01