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    Microtome

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    gandhiayu
    The document discusses the principles and types of microtomy. It describes five basic types of microtomes - rocking, rotary, sledge, sliding, and freezing. It explains how to prepare tissue samples, maintain microtome knives, cut sections, and mount stained sections. Key factors that affect section quality like blunt knives, soft wax, and non-parallel surfaces are also summarized.

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    Attribution Non-Commercial (BY-NC)
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    Microtome

    Uploaded by

    gandhiayu
    353 views32 pages
    The document discusses the principles and types of microtomy. It describes five basic types of microtomes - rocking, rotary, sledge, sliding, and freezing. It explains how to prepare tissue samples, maintain microtome knives, cut sections, and mount stained sections. Key factors that affect section quality like blunt knives, soft wax, and non-parallel surfaces are also summarized.

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    Types of microtomes and their uses

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    © Attribution Non-Commercial (BY-NC)

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    The document discusses the principles and types of microtomy. It describes five basic types of microtomes - rocking, rotary, sledge, sliding, and freezing. It explains how to prepare tissue samples, maintain microtome knives, cut sections, and mount stained sections. Key factors that affect section quality like blunt knives, soft wax, and non-parallel surfaces are also summarized.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    353 views32 pages

    Microtome

    Uploaded by

    gandhiayu
    The document discusses the principles and types of microtomy. It describes five basic types of microtomes - rocking, rotary, sledge, sliding, and freezing. It explains how to prepare tissue samples, maintain microtome knives, cut sections, and mount stained sections. Key factors that affect section quality like blunt knives, soft wax, and non-parallel surfaces are also summarized.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    You are on page 1of 32

    Dr. S.

    Manickam

    Understand

    the principles of microtomy Describe the different types of microtomes and their specific purposes Describe the factors affecting section quality Describe the purpose of adhesives

    Knowledge

    of equipment used Practical experience

    Classified by the manner they are ground: Plane concave Plane concave (Very concave) Plane wedge Biconcave

    Sledge

    microtome

    Celloidin

    section with hard

    Thin
    Vibrates

    tissues

    Frozen

    section Paraffin wax section Rigid Suitable for sledge microtome

    Paraffin

    wax sections Used in sledge & Rocking microtomes

    Cutting edge should be straight line formed by intersection of 2 planes, the cutting facets

    Angle between planes Bevel angle (27 - 32)


    Assessed by seeing under microscope at 100X

    Grinding, renews bevel, restores edge to straight line Belgium black vein Arkansas Carborundum Plate glass with abrasives like aluminium oxide

    Measures 8 x 2 inches

    Polishes

    already sharp edge Hide of rump of horse

    Knife

    sharpening machines

    Facet angle is 35o for routine cutting Teflon reduces resistance during cutting; platinum gives edge of blade strength

    Clamped into welldesigned blade holder

    Mechanical devices for cutting thin uniform slices of tissue (sections) Coined by Charles Chevalier, who perfected it around 1825

    5 Basic types: Rocking Rotary Sledge Sliding Freezing

    Knife

    is fixed Block moves through arc & strikes against knife Adv: Small blocks, simple, long lifetime
    Disadv: Used:

    Lightness

    Paraffin & Frozen sections

    Rotary

    action of handwheel Adv: Heavier, ideal for serial sections, large blocks
    Used:

    Paraffin & celloidin sections

    For very large tissue blocks Knife is fixed Block in steel carriage moves forwards & backwards

    Adv: Heavy, no vibration Disadv: Slower


    Used: Paraffin & Celloidin sections

    Knife

    moves horizontally Block remains fixed


    Used:

    Celloidin sections

    Semi-thin

    and ultrathin sections Resin-embedded specimens Glass or diamond knives Block or knife retraction between strokes

    Other

    microtomes can be modified This gives best results

    sections (3-5) of frozen tissue Rotary microtome in deep freeze cabinet


    Thin

    1/8

    inches on all sides of tissue Edges should be parallel Surface should be trimmed with microtome

    Block

    in block holder Insert knife, check tilt Blocks and Knifes edges should be parallel Trim the surface Set thickness to 6 Cut sections and hold the free end with needle or forceps

    In

    cutting paraffin wax section, each section sticks to the previous one by its edge

    If

    every fifth or tenth section is taken for study

    Cutting makes sections Compressed Creased It should be removed & flattened Float them on warm water Waterbath method Hot stage method Warm slide method

    Albumin

    Starch

    paste Chrome gelatin

    Faults Section cut vertically

    Reasons Knife edge is damaged

    Section rolls up
    Section crumbles on cutting Ribbon curves

    Knife is blunt or tilted more


    Knife blunt, wax is soft Block edges are not parallel to each other or to knife

    Tissue embedded on ice & sections cut on freezing microtome

    To demonstrate fat, lipids or special components For rapid preparation for Dx

    Fixation: Formal saline, 60, 10 min Gum syrup 8 -10 min

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