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    6850/6890 GC Column Installation and Instrument Setup Guide - PPIP/TCD

    Uploaded by

    Waleed El-azab
    This document provides instructions for installing gas chromatography (GC) columns and setting up GC inlets and detectors, including the purged packed injection port (PPIP) and thermal conductivity detector (TCD). It describes how to prepare and install capillary columns, set flows for the PPIP and TCD, and outlines checkout conditions and chromatograms to test the instruments. The document contains detailed steps, diagrams and examples to optimize the GC setup and component configurations.

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    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd

    6850/6890 GC Column Installation and Instrument Setup Guide - PPIP/TCD

    Uploaded by

    Waleed El-azab
    872 views18 pages
    This document provides instructions for installing gas chromatography (GC) columns and setting up GC inlets and detectors, including the purged packed injection port (PPIP) and thermal conductivity detector (TCD). It describes how to prepare and install capillary columns, set flows for the PPIP and TCD, and outlines checkout conditions and chromatograms to test the instruments. The document contains detailed steps, diagrams and examples to optimize the GC setup and component configurations.

    Original Title

    PPIP_TCD Column Install_Setup Guide

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    © © All Rights Reserved

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    This document provides instructions for installing gas chromatography (GC) columns and setting up GC inlets and detectors, including the purged packed injection port (PPIP) and thermal conductivity detector (TCD). It describes how to prepare and install capillary columns, set flows for the PPIP and TCD, and outlines checkout conditions and chromatograms to test the instruments. The document contains detailed steps, diagrams and examples to optimize the GC setup and component configurations.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    872 views18 pages

    6850/6890 GC Column Installation and Instrument Setup Guide - PPIP/TCD

    Uploaded by

    Waleed El-azab
    This document provides instructions for installing gas chromatography (GC) columns and setting up GC inlets and detectors, including the purged packed injection port (PPIP) and thermal conductivity detector (TCD). It describes how to prepare and install capillary columns, set flows for the PPIP and TCD, and outlines checkout conditions and chromatograms to test the instruments. The document contains detailed steps, diagrams and examples to optimize the GC setup and component configurations.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 18

    6850/6890 GC Column Installation and

    Instrument Setup Guide - PPIP/TCD


    July 14, 2004
    Title of Presentation
    Date
    Agilent Restricted Page 2
    GC Setup and Capillary Column Preparation
    Inlets:

    Split/Splitless Capillary Inlet

    PPIP - Purged - Packed Injection Port

    PCOC - Programmable Cool on Column

    PTV - Programmable Temperature Vaporization Inlet
    LVI - Large Volume Injection

    VI - Volatiles Interface

    GSV - Gas Sampling Valve


    Title of Presentation
    Date
    Agilent Restricted Page 3
    GC Capillary Column Preparation
    Created 5//02
    Ceramic Column Cutter
    Rough Edge
    New Column Ferrule
    Column Nut
    Septum to mark
    Column Position
    1) Slide a used Septum over the
    column as a marker
    2) Slide a Column Nut onto the
    column
    3) Slide a new Column Ferrule
    onto the column
    4) Score the column and snap off
    the end
    5) Inspect the column for a clean
    cut.
    Title of Presentation
    Date
    Agilent Restricted Page 4
    GC Inlet Systems - Purged Packed Injection Port
    Maintenance Parts
    Septum Purge Weldment
    O Ring
    Glass Liner Sleeve
    Septum
    1/4 Vespel Seal
    Heater/Sensor
    Assembly
    Column Nut/Ferrule
    Column Adapter
    Septum Purge Weldment
    Title of Presentation
    Date
    Agilent Restricted Page 5
    GC Inlet Systems - Purged Packed Injection Port
    Column Adapters/liners - Capillary Column Installation
    Capillary Packed Glass Insert
    1-2mm
    2
    0
    22
    cm
    Column Adapters/liners Capillary Column Installation
    Title of Presentation
    Date
    Agilent Restricted Page 6
    6890 GC Inlets - Purged Packed Injection
    Port
    Maintenance:
    Replace Septum
    Replace Glass Liner
    Replace Septum Purge Weldment O-Ring
    Perform Inlet Leak Test
    Run Customer Sample or Agilent Checkout
    Replace 1/4 Vespel Ferrule
    Title of Presentation
    Date
    Agilent Restricted Page 7
    Electronic Pneumatic Control.PPIP
    Packed Column/Flow Control Mode -
    Flow can be set, Pressure readout only
    No Column assigned to inlet
    Capillary/Pressure Control Mode -
    Pressure can be set, Flow readout only
    Selected by assigning a capillary column to the inlet (Config Column 1 or 2)
    Title of Presentation
    Date
    Agilent Restricted Page 8
    GC Setup and Capillary Column Installation
    Detectors:

    FID - Flame Ionization Detector

    TCD - Thermal Conductivity Detector

    NPD - Nitrogen Phosphorous Detector

    ECD/uECD - (micro) Electron Capture Detector

    FPD - Flame Photometric Detector


    Title of Presentation
    Date
    Agilent Restricted Page 9
    6850/90 THERMAL CONDUCTIVITY
    DETECTOR
    Gas Connections
    Note: The Reference/Makeup Gas
    must be the same as the Carrier
    Title of Presentation
    Date
    Agilent Restricted Page 10
    6890 TCD Setup -
    Detector Column Adapters
    New Ferrule Design-6850/90
    Original 6890 Design
    Capillary Adapter
    Old Style New Style
    G1532-80540
    0100-1332
    5182-9677- .20/.250
    5182-9676- .320
    5182-9673- .530
    5182-3477
    Description Column ID mm Single part# 10 Pack#
    Back Ferrule for 1/8" inlet G1530-80400 5182-3477
    Frnt Ferr for 0.8mm OD Col 0.53 G1530-80410 5182-9673
    Frnt Ferr for 0.53mm OD Col 0.32 G1530-80420 5182-9676
    Frnt Ferr for 0.43mm+OD Col 0.25/0.20/0.1 G1530-80430 5182-9677
    Front Ferrule, no hole G1530-80440 5182-9679
    Title of Presentation
    Date
    Agilent Restricted Page 11
    6850/90 Setup - TCD Column Installation -
    Original Capillary Adapter
    6890 Operating Manual -
    Volume 1 Chapter 5, Part1
    1. 2.
    3. 4.
    Title of Presentation
    Date
    Agilent Restricted Page 12
    6850/90 Setup - TCD Column Installation -
    New Capillary Adapter
    Column Installation - from 6890N User Information
    1- Slide the 1/8 swagelok Column Nut,
    Back Ferrule and Front Ferrule
    onto the column.

    2- Trim the end of the column

    3- Insert the column into the TCD and
    thread the nut onto the fitting

    4- Push the column all the way into the
    Detector and withdraw 1-2 mm.

    5- Tighten the column nut
    1/8 Swagelok
    Title of Presentation
    Date
    Agilent Restricted Page 13
    6850/90 TCD - Front Panel Menu
    Turns Filament On and Off
    Output of Detector. 1 unit = 25uV
    Turns polarity between + and -
    6890 TCD OPERATION
    Title of Presentation
    Date
    Agilent Restricted Page 14
    6850/90 THERMAL CONDUCTIVITY
    DETECTOR
    Optimizing Makeup and Reference Flows
    0510152025303540455055606570
    0.5
    1
    1.5
    2
    2.5
    3
    3.5
    Column+Makeupflow(ml/min)
    R a t i o o f r e f f l o w t o M U + C o l f l o w
    Thermal ConductivityReferenceGasFlow
    ColumnFlow=25
    Refflow=2X25=50
    Title of Presentation
    Date
    Agilent Restricted Page 15
    6850/90 TCD - Optimizing Makeup and
    Reference Flows
    Setting TCD Flows in Packed Mode

    The Packed Mode describes using the TCD with column flow rates between 15 and 60
    ml/min. The reference flow should be set to 1.5 times the combined flow rate of the
    column plus make-up gas. Make-up gas is recommended whenever a capillary column
    is used. This allows the column to be installed all the way into the detector and
    withdrawn one millimeter. If make-up is not used then the end of the column should be
    no more than three millimeters above the ferrule. Otherwise excessive tailing will occur.
    The minimum make-up flow is 1 ml/min.

    .Example 1: 1/8 Stainless Steel Column If the column flow = 30 ml/min, then the
    reference flow should be set to (30ml/min) (1.5) = 45 ml/min. Therefore total detector
    flow is 30 + 45 = 75 ml/min.

    .Example 2: 10 Meter X 530 uM column If the column flow = 15 ml/min and the
    make-up flow = 2 ml/min, then the reference flow should be set to (15 ml/min + 2
    ml/min) (1.5) = 25.5 ml/min. Therefore the total detector flow is 17 + 25.5 = 42.5 ml/min.


    Title of Presentation
    Date
    Agilent Restricted Page 16
    6850/90 TCD - Optimizing Makeup and
    Reference Flows
    Setting Flows in Capillary Mode

    The Capillary Mode describes using the TCD with a combined column plus make-up
    flow of less than 15 ml/min. The combined column plus make-up flow must not be
    less than 5 ml/min. The reference flow rate should be set to optimize detector
    response. This will result in a reference flow of approximately two times the combined
    column plus make-up flow for a column plus make-up flow of 10-15 ml/min. For a column
    plus make-up flow of 5 - 10 ml/min, a multiplier of three is used. The detector output will
    be within 25% of the maximum response. The response can be further optimized by
    adjusting the reference flow. The make-up flow should not be less than 1 ml/min.

    .Example 3: 2 Meter X 200 uM capillary column If the column flow rate = .75 ml/min,
    then the make-up flow must be at least 4.25 ml/min. Set the make-up flow to 5 ml/min.
    The optimum reference flow will be (5.75 ml/min) (3) = 17.25 ml/min. The total detector
    flow will be 5.75 + 17.25 = 22.5 ml/min.

    .Example 4: 25 Meter X 320 uM capillary column if the column flow rate = 10
    ml/min, then the make-up flow should be set low to minimize sample dilution. Set the
    make-up to 2 ml/min. The reference flow will = (12 ml/min) (2) = 24 ml/min. The total
    detector flow will be 12 + 24 = 36 ml/min.

    Title of Presentation
    Date
    Agilent Restricted Page 17
    6850/90 TCD Checkout Conditions
    Title of Presentation
    Date
    Agilent Restricted Page 18
    6850/90 TCD Checkout Chromatogram
    25 uV
    (Sampling Rate)
    0
    10
    20
    30
    40
    50
    60
    70

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