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Types of Biopsy

• Surgical biopsy- Incisional Biopsy ,Excisional Biopsy and


Punch Biopsy.
• Fine Needle Aspiration Cytology(FNAC) and CT guided
FNAC.
• Exfoliative Cytology.
• Brush Biopsy.
• Frozen Section Biopsy.
• Core Needle Biopsy. trucut biopsy
STEPs OF BIOPSY

• 1.SELECTION OF AREA OF BIOPSY


• 2.PREPARATION OF SURGICAL FIELD
• 3.LOCAL ANASTHESIA
• 4.INCISION
• 5.HANDLING OF SPECIMEN
• 6.SUTURING OF THE RESULTING WOUND
INCISIONAL BIOPSY

If a lesion is large or has different


characteristics in various locations
more than one area may need to be
sampled
Incision should extend from the ulceration out onto clinically normal tissue

Grasp area to be removed with forceps and make an elliptical incision from the centre out
onto clinically normal tissue: wound after removal of incised tissue: suturing completed
Incisional Biopsy
• Indications:
• Size limitations
• Hazardous location of the lesion
• Great suspicion of malignancy

• Technique:
• Representative areas are biopsied in a wedge fashion.
• Margins should extend into normal tissue on the deep surface.

• Necrotic tissue should be avoided.


• A narrow deep specimen is better than a broad shallow one.
DISADVANTAGES:
1. Crush, splits and haemorrhage are the artefacts most
frequently found in incisional oral biopsies.
2. Theoretical seeding of cancer cells into the adjoining tissues.
Excisional Biopsy
The entire lesion with 2 to
3mm of normal appearing
tissue surrounding the
lesion is excised if benign.
Excisional Biopsy

An excisional biposy implies the complete removal of the


lesion.
• Indications:
• Should be employed with small lesions. Less than 1cm
• The lesion on clinical exam appears benign.
• When complete excision with a margin of normal tissue is
possible without mutilation.
Excisional Biopsy

• Technique:
• The entire lesion with 2 to 3mm of normal appearing
tissue surrounding the lesion is excised if benign.

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