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    Simmons Citrato

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    Karen Flores
    This document describes Simmons Citrate agar, a differential culture medium used to differentiate Gram-negative bacteria based on their ability to use citrate as a sole carbon source. It contains trisodium citrate as the sole carbon source and ammonium dihydrogen phosphate as the sole nitrogen source. Bacteria that are able to metabolize citrate through the citric acid cycle will alkalinize the medium, turning it blue. Common citrate-positive bacteria include Enterobacter, Klebsiella, Serratia, and Citrobacter, while Escherichia, Shigella, Salmonella typhi and Salmonella paratyphi are typically citrate-negative. The test determines if an organism can use citrate

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    Simmons Citrato

    Uploaded by

    Karen Flores
    337 views2 pages
    This document describes Simmons Citrate agar, a differential culture medium used to differentiate Gram-negative bacteria based on their ability to use citrate as a sole carbon source. It contains trisodium citrate as the sole carbon source and ammonium dihydrogen phosphate as the sole nitrogen source. Bacteria that are able to metabolize citrate through the citric acid cycle will alkalinize the medium, turning it blue. Common citrate-positive bacteria include Enterobacter, Klebsiella, Serratia, and Citrobacter, while Escherichia, Shigella, Salmonella typhi and Salmonella paratyphi are typically citrate-negative. The test determines if an organism can use citrate

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    This document describes Simmons Citrate agar, a differential culture medium used to differentiate Gram-negative bacteria based on their ability to use citrate as a sole carbon source. It contains trisodium citrate as the sole carbon source and ammonium dihydrogen phosphate as the sole nitrogen source. Bacteria that are able to metabolize citrate through the citric acid cycle will alkalinize the medium, turning it blue. Common citrate-positive bacteria include Enterobacter, Klebsiella, Serratia, and Citrobacter, while Escherichia, Shigella, Salmonella typhi and Salmonella paratyphi are typically citrate-negative. The test determines if an organism can use citrate

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    337 views2 pages

    Simmons Citrato

    Uploaded by

    Karen Flores
    This document describes Simmons Citrate agar, a differential culture medium used to differentiate Gram-negative bacteria based on their ability to use citrate as a sole carbon source. It contains trisodium citrate as the sole carbon source and ammonium dihydrogen phosphate as the sole nitrogen source. Bacteria that are able to metabolize citrate through the citric acid cycle will alkalinize the medium, turning it blue. Common citrate-positive bacteria include Enterobacter, Klebsiella, Serratia, and Citrobacter, while Escherichia, Shigella, Salmonella typhi and Salmonella paratyphi are typically citrate-negative. The test determines if an organism can use citrate

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    SIMMONS CITRATO

    COMPOSICION(por litro) Descripción del Medio


     Tri-sodio citrato 2.0 g Requerimiento Energético
     Amonio di-hidrogeno fosfato 1.0 g Fuente de carbono: citrato
     Azul de bromotimol 0.08 g Fuente de nitrógeno: amonio
     Magnesio sulfato 0.2 g Requerimiento no Energético
     Di-potasio hidrogeno fosfato 1.0 g Fuente de azufre: sulfató de magnesio MgSO4
     Sodio cloruro 5.0 g Fuente de fósforo: fosfato dipotásico K2HPO4
     Agar 15.0 g Iones metálicos: cloruro de sodio NaCl
    Preparación Factores inhibidores: no tiene
    Suspendemos 0.56 g en 25 ml de Según su proporción en agua medio solido
    agua destilada: calentamos y Según su uso o utilización medio diferencial
    agitamos hasta ebullición y hervir Según la composición medio definido
    durante 1 min. Distribuir y Según su presentación tubos de ensayo
    esterilizar a 121 oC durante 15 Almacenamiento:
    minutos.  Medio de cultivo deshidratado a 10 – 35 oC
    La distribución en tubos de ensayo  Medio de cultivo preparado a 2 – 8 oC
    deja enfriar en posición inclinada
    PICO.
    Siembra: Estría simple superficie del medio
    Incubación: En aerobiosis a 35 – 37 oC durante 18 - 24horas
     Es un medio de cultivo utilizado para diferenciar las
    bacterias Gram negativas en función de su capacidad de
    utilizar el Citrato como única fuente de Carbono.
     Esta prueba sirve para determinar si un organismo es capaz
    de utilizar citrato como única fuente de carbono y Negativas
    compuestos amoniacales como única fuente de nitrógeno Escherichia,
    en su metabolismo, provocando una alcalinización del Shigella, Salmonella
    medio. typhi y Salmonella
     El metabolismo del citrato se realiza , en aquellas bacterias paratyphi son
    poseedoras de citrato permeasa, a través del ciclo del acido incapaces de crecer
    Entre las
    tricarboxilico. con esos nutrientes.
    enterobacterias
     El desdoblamiento del citrato genera progresivamente,
    positivas oxalacetato y piruvato.
    Enterobacter,  Piruvato en medio alcalino, da origen a ácidos orgánicos que al
    Klebsiella, Serratia, ser utilizados como fuente de carbono, producen carbonatos y
    Citrobacter y algunas bicarbonatos alcalinos. El medio entonces vira a azul y esto es
    especies indicativo de la producción de citrato permeasa .
    de Salmonella.
    VERDE pH del medio : 6.9 -
    AZUL pH del medio alcalino: 7.6 +

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