Enzymes PPT by Utkarsh Satish

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The key takeaways are that enzymes are proteins that catalyze chemical reactions in cells and organisms. They speed up reactions and minimize the energy needed to achieve reactions. There are over 2000 known enzymes, each involved in a specific reaction.

Enzymes are protein molecules found in cells that catalyze chemical reactions. They are catalysts that optimize cell activity while minimizing energy needed for reactions. Enzymes are necessary for life and each is involved in one specific reaction. The pancreas produces enzymes that break down food into nutrients.

Enzymes function by binding to substrates at their active site. The shape of the active site fits the substrate like a lock and key. This allows only the correct substrate to bind. Once bound, the enzyme can promote the reaction by orienting substrates, weakening bonds, or coupling reactions.

Enzymes PPT by Utkarsh Satish

Class:- 8IG-A
What are Enzymes?
Enzymes are catalysts that optimize cell activity while minimizing
the amount of energy needed to achieve a specific reaction.
Enzymes are also energized protein molecules found in every living
cell, and are necessary for life. There are over 2000 known enzymes,
each of which is involved with one specific chemical reaction. There
many various proteins, originating from living cells and capable of
producing certain chemical changes in organic substances by
catalytic action, such as digestion. These proteins, and their
functions, are determined by their shape
In cells and organisms, most reactions are catalyzed by enzymes,
which are regenerated during the course of a reaction. Biological
catalysts are physiologically important because they speed up rates
of reactions that would otherwise be too slow to support life. Our
bodies naturally produce digestive and metabolic enzymes as they
are needed. Specifically, the pancreas produces enzymes that break
down foods into nutrients the body can use for energy and other
bodily functions.
Functions of Enzymes :-
In simple terms, an enzyme functions by binding to one or more of the reactants in a
reaction. The reactants that bind to the enzyme are known as the substrates of the
enzyme. The exact location on the enzyme where substrate binding takes place is
called the active site of the enzyme. The shape of the active site just fits the shape of
the substrate, somewhat like a lock fits a key. In this way only the correct substrate
binds to the enzyme.
Once the substrate or substrates are bound to the enzyme, the enzyme can promote
the desired reaction in some particular way. What that way is depends on the nature
of the reaction and the nature of the enzyme. An enzyme may hold two substrate
molecules in precisely the orientation needed for the reaction to occur. Or binding
to the enzyme may weaken a bond in a substrate molecule that must be broken
in the course of the reaction, thus increasing the rate at which the reaction can
occur.
An enzyme may also couple two different reactions. Coupling an exothermic
reaction with an endothermic one allows the enzyme to use the energy released by
the exothermic reaction to drive the endothermic reaction. In fact, a large variety of
enzymes couple many different endothermic reactions to the exothermic reaction in
which ATP is converted by hydrolysis to ADP. In this way, ATP serves as the
molecular fuel that powers most of the energy-requiring processes of living things.
Effects of PH :-

Enzymes are affected by changes in


ph. The most favorable pH value , the
point where the enzyme is most
active, is known as the optimum ph.
Extremely high or low pH values
generally result in complete loss of
activity for most enzymes. pH is also
a factor in the stability of enzymes.
As with activity, for each enzyme
there is also a region of pH optimal
stability.
In addition to temperature and pH
there are other factors, such as ionic
strength, which can affect the
enzymatic reaction. Each of these
physical and chemical parameters
must be considered and optimized in
order for an enzymatic reaction to be
accurate and reproducible.
Differing pH value of Enzymes

 The following table shows the different pH levels of


some Enzymes :-
Enzyme pH Optimum
Lipase 8.0
(pancreas)
Lipase (stomach) 4.0 - 5.0
Lipase (castor oil) 4.7
Pepsin 1.5 - 1.6
Trypsin 7.8 - 8.7
Urease 7.0
Invertase 4.5
Maltase 6.1 - 6.8
Amylase (pancreas) 6.7 - 7.0

Amylase (malt) 4.6 - 5.2


Catalase 7.0

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