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    Microscope Basics

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    Mark Arnold Malaluan
    Microscopes magnify objects too small to see with the naked eye using lenses. There are three main types: simple microscopes with one lens, compound microscopes with two lens sets magnifying up to 200x, and electron microscopes using electrons to magnify up to 300,000x. Microscopes have many parts including ocular lenses, objectives, stages, and bases. Users must handle them carefully and can calculate magnification by multiplying ocular and objective powers. Wet mounts are made by placing specimens in a water drop covered with a slip for viewing.

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    Microscope Basics

    Uploaded by

    Mark Arnold Malaluan
    138 views15 pages
    Microscopes magnify objects too small to see with the naked eye using lenses. There are three main types: simple microscopes with one lens, compound microscopes with two lens sets magnifying up to 200x, and electron microscopes using electrons to magnify up to 300,000x. Microscopes have many parts including ocular lenses, objectives, stages, and bases. Users must handle them carefully and can calculate magnification by multiplying ocular and objective powers. Wet mounts are made by placing specimens in a water drop covered with a slip for viewing.

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    Microscopes magnify objects too small to see with the naked eye using lenses. There are three main types: simple microscopes with one lens, compound microscopes with two lens sets magnifying up to 200x, and electron microscopes using electrons to magnify up to 300,000x. Microscopes have many parts including ocular lenses, objectives, stages, and bases. Users must handle them carefully and can calculate magnification by multiplying ocular and objective powers. Wet mounts are made by placing specimens in a water drop covered with a slip for viewing.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    138 views15 pages

    Microscope Basics

    Uploaded by

    Mark Arnold Malaluan
    Microscopes magnify objects too small to see with the naked eye using lenses. There are three main types: simple microscopes with one lens, compound microscopes with two lens sets magnifying up to 200x, and electron microscopes using electrons to magnify up to 300,000x. Microscopes have many parts including ocular lenses, objectives, stages, and bases. Users must handle them carefully and can calculate magnification by multiplying ocular and objective powers. Wet mounts are made by placing specimens in a water drop covered with a slip for viewing.

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    You are on page 1of 15

    MICROSCOPES

    • Magnifies objects (makes objects


    look bigger)
    • Help scientists study objects & living
    things too small to see with the naked
    eye
    Ocular lens
    (Eyepiece)
    Body Tube

    Nosepiece
    Arm
    Objectives

    Stage
    Stage Clips
    Coarse Adjustment
    Diaphragm
    Fine Adjustment
    Light

    Base
    Always carry a microscope with one hand
    holding the arm and one hand under the base.
    3 Types of Microscopes
    • simple microscope has only 1 lens.
    • compound microscope has 2 sets of
    lenses. It can magnify things 100 -
    200 times larger than they really are.
    • electron microscope can magnify
    objects up to 300,000 times. They
    do not use lenses, but use electrons
    to enlarge the image.
    PARTS OF A MICROSCOPE
    1. ocular (lens) eyepiece
    • the lens of the microscope that you
    look through
    2. coarse adjustment
    • the large knob on the microscope
    that moves the body tube up or
    down to bring the object into focus
    3. fine adjustment
    • the small knob on the microscope
    that brings the image into sharp
    focus
    PARTS OF A MICROSCOPE

    4. arm
    • the part of the microscope
    supporting the body tube
    5. body tube
    • the part that holds the eyepiece and
    the objective lenses.
    6. nosepiece
    • the part at the bottom of the body
    tube that holds the objective lenses
    and allows them to be turned
    7. high power objective lens
    • the lens that magnifies the object
    the greatest amount. (usually 40x)
    8. Low power (scanner) objective lens
    • the lens that magnifies the object
    the least amount (usually used to find
    the object; magnifies only 3x or 4x)

    9. middle power objective lens


    • the lens that usually magnifies the
    object more than the scanner lens,
    but less than the high power lens
    (usually 10x to 20x)
    10. stage
    • the flat part below the
    objectives lens where the slide
    is placed
    11. clip
    • the part that holds the slide in
    place so it doesn’t move
    12. diaphragm
    • the part that controls the
    amount of light entering the
    field of view
    13. light source
    • the lamp (or mirror) under the
    stage that sends light through
    the object being viewed.
    14. base
    • the bottom part that supports
    the rest of the microscope
    Ocular lens
    (Eyepiece)
    Body Tube

    Nosepiece
    Arm
    Objectives

    Stage
    Stage Clips
    Coarse Adjustment
    Diaphragm
    Fine Adjustment
    Light

    Base
    Always carry a microscope with one hand
    holding the arm and one hand under the base.
    Comparing Powers of Magnification

    We can see better details with higher the


    powers of magnification, but we cannot see
    as much of the image.

    Which of these images


    would be viewed at a
    higher power of
    magnification?
    What’s my power?
    To calculate the power of magnification, multiply the power of the
    ocular lens by the power of the objective.
    Appearance of the Specimen
    • Objects appear upside-down &
    backward

    • Movement appears to be in opposite


    direction than actual movement 1
    2

    move slide
    slide appears to move
    2 1
    Making a Wet Mount Slide
    1. Use dropper to place a drop of
    water on the center of a clean slide.
    2. Use tweezers to lay specimen on
    the drop of water.

    3. Gently touch the cover slip to the


    edge of the drop of water to cover
    the specimen & the water.
    How to make a wet-mount slide …
    1 – Get a clean slide and coverslip from your teacher.
    2 – Place ONE drop of water in the middle of the slide. Don’t use
    too much or the water will run off the edge and make a mess!
    3 – Place the edge of the cover slip on one side of the water drop.
    4 - Slowly lower the cover slip on top of the drop.
    Cover Lower slowly
    Slip

    5 – Place the slide on the stage and view it first with the red-banded
    objective. Once you see the image, you can rotate the nosepiece to
    view the slide with the different objectives.
    You do not need to use the stage clips
    when viewing wet-mount slides!

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