MICROSCOPE

The microscope gets its
name from the Greek

words micro, meaning small,
and skopion, meaning to see
or look, and it literally is a
machine for looking at
small things.
HISTORY OF PARTS OF THE
THE COMPOUND
MICROSCOPE MICROSCOPES
MICROSCOPE
PROPER
FUNCTIONS
HANDLING OF
OF THE
THE
COMPOUND
COMPOUND
MICROSCOPES
MICROSCOPES
The microscope
was first built in
1595 by Hans and
Zacharias Janssen
(1588-1631) in
Holland
Later, it was perfected
in the 17th century in
several countries,
including by Robert
Hooke (1635-1703), in
England but most
notably by a Dutchman,
Anton Van
Leeuwenhoek.
Robert Hooke using a
much improved
microscope, with a
monocular eyepiece, a
wooden tube, a stage
for holding a specimen,
and a glass globe full
of water to concentrate
light onto it. Hooke
produced marvelous
illustrations, whic were
published in 1667.
• Anton Van
Leeuwenhoek
(1632-1723) was
the first to see
bacteria, yeast, and
life found in a drop
of pond water.
•In approx. 1880 =
modern microscopes
were being used
• with the
advancecement of
technology and
improved optics, the
compund LIGHT
MICROSCOPE came
into being
Electron Microscope
• Developed in the
1930
• Allowed for higher
magnification
•Used electron beams
(instead of light) and
focused with an
electromagnet (no
lenses)
•Light microscope
magnifies up to
2000X
• Electron microscopes
magnifies up to
50000X or higher
•Better quality images
at higher
magnification
1. Eyepieces are the lenses
at the top that the
viewer looks through;
they are usually 10X or
15X.
2. Body Tube, The long
tube that holds the
eyepiece and connects
the objective
3. Nosepiece, Rotating
part of the microscope
at the bottom of the
body tube. It holds the
objective lenses
4. Arm, The part of
the microscope that
connects the
eyepiece tube to the
base.
5. Objective
Lenses:
• High Power
Objective
• Low Power
Objective
• Mid Power
Objective
6. Stage : The flat
platform where
you place your
slides. It has a
hole in it so light
can shine
through.
7. Stage Clip: Shiny
clips on the top of
the stage. Holds a
slide in place
8. Diaphragm: Is
used to adjust the
amount of light
and is located in
the base
9. Coarse Adjustment:
Large, round knob on
the side of the
microscope. Either
moves the stage or the
top parts of the
microscope up and
down
10. Fine
Adjustment:
Small, round knob
on the side of the
microscope. Used
to fine tune the
focuse after using
the coarse
adjustment knob
11. Light Source
Microscope: Any
microscope that uses a
source of light to create
an image of the
specimen and,
essentially includes all
compound and stereo
microscopes.
12. Base: The bottom
of the microscope,
used for support. Hold
this part with one
hand when carrying a
microscope
2 Column notepad
Parts of a Microscope Function
Eyepiece
Fine Adjustment
• You have to make a table like this on the paper

• Give 2 parts of a microscope, for the example....
• Please provide your table to beside you
• You must answer the function of the microscope from the other
table you get
1.When moving your
microscope, always
carry it with both
hands (Figure 1, at
left). Grasp the arm
with one hand and
place the other hand
under the base for
support.
2. Turn the revolving
nosepiece so that the
lowest power objective
lens is "clicked" into
position (This is also the
shortest objective lens).
3. Your microscope slide should be
prepared by placing a coverslip or
cover glass over the specimen. This
will help protect the objective
lenses if they touch the slide. Place
the microscope slide on the stage
and fasten it with the stage
clips. You can push down on the
back end of the stage clip to open it.
4. Look at the objective
lens and the stage from
the side (Figure 2, at
right) and turn the coarse
focus knob so that the
objective lens moves
downward (or the stage,
if it moves, goes upward).
Move it as far as it will
go without touching the
slide!
5. Now, look through
the eyepiece and
adjust the
illuminator (or
mirror) and
diaphragm (Figure
3,at left) for the
greatest amount of
light.
6. Slowly turn the coarse adjustment so
that the objective lens goes up (away
from the slide). Continue until the
image comes into focus. Use the fine
adjustment, if available, for fine
focusing. If you have a microscope
with a moving stage, then turn the
coarse knob so the stage moves
downward or away from the objective
lens.
7. Move the microscope
slide around so that the
image is in the center of the
field of view and readjust
the mirror, illuminator or
diaphragm for the clearest
image.
8. Now, you should be able to
change to the next objective lens
with only minimal use of the
focusing adjustment. Use the fine
adjustment, if available. If you
cannot focus on your specimen,
repeat steps 4 through 7 with the
higher power objective lens in
place.
9. The proper way to use a monocular
microscope is to look through the
eyepiece with one eye and keep the
other eye open (this helps avoid eye
strain). If you have to close one eye
when looking into the microscope, it's
ok. Remember, everything is upside
down and backwards. When you move
the slide to the right, the image goes to
the left.
10. Do not touch the
glass part of the lenses
with your fingers. Use
only special lens paper
to clean the lenses.
11. When finished, raise
the tube (or lower the
stage), click the low
power lens into
position and remove
the slide.
12. Always keep
your microscope
covered when
not in use.
#Reflection
1. How can you become
a light to others,
especially those in
need?
#Reflection
2. Are there moments in
your life that we are
likened to a microscope?
Cite some instances?
#Reflection
3.What should be
magnified, good deeds or
bad deeds? Justify your
answer.
QUOTE OF THE DAY
• Group Activity

MICROSCOPE

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MICROSCOPE

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The microscope gets its

name from the Greek

Parts of a Microscope Function

• You have to make a table like this on the paper

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