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    3-1 Dynamic Light Scattering

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    Amal Patel
    Dynamic Light Scattering (DLS) is a non-invasive technique used to determine the size of particles and molecules in suspension by measuring their Brownian motion. Smaller particles move more rapidly due to bombardment by solvent molecules, while larger particles move more slowly. DLS converts the measured translational diffusion coefficient into hydrodynamic size, which is the size of a hypothetical hard sphere that diffuses at the same rate as the particle. An experiment was conducted using DLS to determine the z-average size, polydispersity index (PDI), and zeta potential (ZP) of polystyrene and ZIF-8 samples.

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    3-1 Dynamic Light Scattering

    Uploaded by

    Amal Patel
    559 views5 pages
    Dynamic Light Scattering (DLS) is a non-invasive technique used to determine the size of particles and molecules in suspension by measuring their Brownian motion. Smaller particles move more rapidly due to bombardment by solvent molecules, while larger particles move more slowly. DLS converts the measured translational diffusion coefficient into hydrodynamic size, which is the size of a hypothetical hard sphere that diffuses at the same rate as the particle. An experiment was conducted using DLS to determine the z-average size, polydispersity index (PDI), and zeta potential (ZP) of polystyrene and ZIF-8 samples.

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    Dynamic Light Scattering (DLS) is a non-invasive technique used to determine the size of particles and molecules in suspension by measuring their Brownian motion. Smaller particles move more rapidly due to bombardment by solvent molecules, while larger particles move more slowly. DLS converts the measured translational diffusion coefficient into hydrodynamic size, which is the size of a hypothetical hard sphere that diffuses at the same rate as the particle. An experiment was conducted using DLS to determine the z-average size, polydispersity index (PDI), and zeta potential (ZP) of polystyrene and ZIF-8 samples.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    559 views5 pages

    3-1 Dynamic Light Scattering

    Uploaded by

    Amal Patel
    Dynamic Light Scattering (DLS) is a non-invasive technique used to determine the size of particles and molecules in suspension by measuring their Brownian motion. Smaller particles move more rapidly due to bombardment by solvent molecules, while larger particles move more slowly. DLS converts the measured translational diffusion coefficient into hydrodynamic size, which is the size of a hypothetical hard sphere that diffuses at the same rate as the particle. An experiment was conducted using DLS to determine the z-average size, polydispersity index (PDI), and zeta potential (ZP) of polystyrene and ZIF-8 samples.

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    Dynamic Light Scattering

    Dynamic Light Scattering(DLS) is a non-invasive technique for measuring the size of particles
    and molecules in suspension.
    • Particle/molecular size
    • Size distribution

    DLS doesn’t measure the size of the particles directly, it measures the Brownian motion of
    the particles and uses this information to determine their hydrodynamic size.
    Brownian Motion
    Brownian motion: random movement of particles which results from the bombardment of
    collision of the solvent molecules.

    • Related to size of particles


    • Related to viscosity of the suspension
    • Related to temperature

    -smaller particles moves or diffuses more rapidly


    -larger particles moves or diffuses more slowly
    -velocity of the Brownian Motion can be quantified as
    the translational diffusion coefficient(D)
    Hydrodynamic Size
    The translational diffusion coefficient(D) can be converted to the size of a particle using
    Stoke-Einstein equation

      𝑘 𝐵𝑇
    𝐷𝐻 =
    3 𝜋𝜂 𝐷

     : Boltzman’s constant
    : absolute temperature
    : viscosity
    : hydrodynamic size

    Hydrodynamic size: the size of a sphere that diffuses/moves at the same speed as the
    particle being measured.
    Dynamic Light Scattering
    How does DLS work?

    https://www.youtube.com/watch?v=ET6S03GeMKE&t=181s
    DLS experiment
    Determine and study the z-average, Polydispersity index(PDI), and zeta-potential(ZP) of a
    polystyrene(PS) standard and zeolitic imidazolate framework-8 (ZIF-8) samples.

    z-average: represent the hydrodynamic parameter or the average size of the particle for the
    sample.

    PDI: describes the size distribution. PDI values between 0.0~0.3 are considered acceptable
    by industrial standards, but values greater than 0.7 are not compatible with DLS.

    ZP: describes the surface charge of the particle in a colloidal. An indicator of stability of
    particles in the colloidal. Very negative(<-60 mv) or very positive(>+60 mv) ZP indicate
    excellent stability(particles repel each other due to high voltages with the same sign)

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