Operating Microscope: Submitted By: Meisha Imam Roll No: BSMMG38F17 Submitted To: Dr. Mehboob Ahmed

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Operating

Microscope
Submitted by: Meisha Imam
Roll No: BSMMG38F17
Submitted to: Dr. Mehboob Ahmed 
Background

 Various diseases, including cancer, require surgery as a prime treatment method.


 One key actor for surgeons to operate accurately is a clear visualization of the
anatomical structures.
 This has never been easy. some anatomical structures are very small, varying from
millimeters to microns.
 They might have close proximity to other organs or tissue
 A clear view of these structures requires a resolution well beyond that of  human
eyes.
 Lack of illumination in narrow cavities and deep channels results in a dim
visualization with shadows
Continued

 Poor visualization may lead to inappropriate operation on anatomical structures or


a nearby organ.
 This which will affect the surgical outcome, reduce organ preservation, or even
cause life-threatening consequences.
 Sufficient magnification and proper illumination are vital for the success of
surgery.
 Before the advent of the surgical microscope, surgeons had been using various
magnifying systems mounted on spectacles or headbands.
History

 In 1590, compound microscope had been invented  and was used for examination
of wounds and scars in the late 17th century, it had several limitations.
 Carl Zeiss company presented a binocular loupe with a working distance of 25 cm,
which opened the door to modern microsurgery.
 In the late 19th century, Ernst Abbe proposed numerical aperture and greatly
enhanced the resolution of microscopes.
 In 1921 a monocular microscope truly entered the operating room for an aural
surgery.
 One year later, this idea was modified using a binocular microscope.
Introduction

 A surgical microscope, also known as an operating microscope, is an optical


microscope
 It is specifically designed to be used in a surgical setting.
 It is especially requisite for microsurgery.
 Surgical microscopes provide adjustable magnification, bright illumination, and
clear visualization
Earliest
Operating
Microscopes
(a) Brinell–Leitz
monocular microscope used
by Carl Olof Nylen and the
modified Brinell microscope
 (b) Zeiss binocular
microscope adapted by
Holmgren
Zeiss
company's
Microscope 
Zeiss-Opton microscope by
Hans Littman had various
magnification options and
one working distance.
Revolutionized

Operating
Microscope
Surgical microscope Zeiss OPMI
1:
(a) Zeiss OPMI 1 on its stand
with motorized head,
(b) Zeiss OPMI 1 with a camera,
and
(c) Zeiss OPMI 1 optical
diagram.
Revolutionized 
Operating 
Microscope
Surgical microscope OPMI 2
and OPMI 3
Components

 A surgical microscope can be roughly divided into a microscope body, a light


source, and a supporting structure.
 Each of these is vital for the performance of the microscope.
 Modern microscopes have adopted advanced technologies to facilitate
visualization and surgical navigation.
Microscope body

 It has all the high-precision optics that provide a clear magnified image with the
minimum distortion.
 The binoculars mounted on the microscope head offer stereopsis.
 Multiple optical ports are open for adaptation of imaging devices.
 These imaging devices can be video cameras or for assistants to share the
identical FOV.
Microscope
body
Light source 

 It is installed away from the microscope to avoid heating the microscope optics or
the surgical site.
 Commonly used light sources for surgical microscope are:
 xenon light bulbs
 halogen light bulbs 
 light-emitting diodes (LED)
Supporting structure 

 Based on the configuration, there are four types of surgical microscopes:


 on casters
 wall mounted 
 table top
 ceiling mounted.
 Supporting structure has precision motorized mechanics
 The microscope can be balanced easily and adjusted flexibly to the right position
with the help of these motors
Supporting
structure 
Magnification and Resolution
 Magnification
 The total magnification (Mtotal) of a surgical microscope is determined by all the four optical
components in the microscope
 the focal length of the objective lens (fOBJ)

 zoom value (MZOOM) 

 the focal length of binocular (fTUBE)

 the magnifying power of eyepieces (MEP)

 Resolution
 Human eyes have an inherent resolution of 0.2 mm 
 With 20× magnification it can be increased to 0.01 mm
Principle

 The basic principle of the microscope is two convex lenses ( objective lens and eyepiece
lens) 
 These are separated to a certain distance more than the focal length of each lens.
 The primary requisite for the good operating microscope is smooth magnification without
any change in working distance. 
 Working distance is the distance between the objective lens and the main focusing point. 
 For the smooth transition of this magnification, the operating microscope is fitted with a
magnification changer based on the principle of the Galilean telescope .
Principle of
Operating
Microscope
Specimen Preparation

 The speciman in this case are the organs and the tissues which are observed live
under the microscope.
 Various fluorescent dyes have been invented for different surgical applications and
put into use. 
 Most invented dyes generate visible fluorescence,  surgeons are provided with a
high contrast of visualization, and they are able to separate different tissues with
human eyes. 
 Besides the visible exogenous fluorescence, some dyes that emit fluorescence in
the NIR wavelength range, such as the ICG, are also helpful during surgery.
Image

 The intraoperative imaging modalities have been evaluated with surgical


microscopes to provide real-time diagnostic information.
 It helps surgeons relate the preoperative twodimensional (2D) images with the
real 3D surgical site intraoperatively for navigation. 
 The overlay of images reveals not only the 3D model but also the anatomical
structures beneath the patient’s skin.
Information provided 

 Operating microscope helps in the visualization of the surgery being performed.


 A surgical microscope is able to provide a clear, bright, and magnified view of the
surgical site
 This also  allow photography or video to be taken of the operating field.
 plastic surgery
 dentistry (especially endod  Spine Surgery
ontics)  Neurovascular surgery
 root canal therapy  Aneurysms
Uses  ENT surgery  Moyamoya disease
Fields of medicine that  ophthalmic surgery  Stroke
make significant use of  Neurosurgery.  Otology
the operating  brain tumor resection  Rhinology
microscope include:  Thyroidectomy  Laryngology
 retinal detachment  Head and neck surgery
 lymphaticovenular
anastomosis (LVA)
Availability in Pakistan and price 

 In Pakistan the operating microscopes are nearly available at all following places
where either surgeries or compelete observation of the eye and ear are made:
 hospitals 
 dental clinics
 Aopthalmology clinics
 The price of an operating microscope can range from $10,000 to $40,000
depending upon the advanced features it have.
Research use

 The further research and advancement can help in directly visualizing the bacteria
and viruses in the organs, tissues of the human body.
References

 http://www.eophtha.com/posts/a-beginners-guide-to-ophthalmic-surgical-micros
cope#:~:text=The%20basic%20principle%20of%20the,any%20change%20in%20
working%20distance
.
 https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-2
6/issue-01/010901/Comprehensive-review-of-surgical-microscopes--technology-d
evelopment-and-medical/10.1117/1.JBO.26.1.010901.full?SSO=1
 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069782/
 https://en.wikipedia.org/wiki/Operating_microscope#:~:text=An%20operating%2
0microscope%20or%20surgical,setting%2C%20typically%20to%20perform%20m
icrosurgery.&text=Additionally%20specialized%20microsurgical%20instruments
%20may,improved%20vision%20the%20microscope%20affords
.

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