The BioRad D10 uses high performance liquid chromatography to separate hemoglobin in whole blood samples into types based on ionic interactions. It dilutes blood samples, runs a buffer gradient to increase ionic strength, and measures separated hemoglobin passing through a flow cell at 415 nm. Whole blood must be collected in EDTA tubes and can be stored for up to 7 days at 2-8 degrees Celsius. Reagents must be inspected for discoloration, cloudiness, precipitation, or leakage and discarded if issues are observed.
The BioRad D10 uses high performance liquid chromatography to separate hemoglobin in whole blood samples into types based on ionic interactions. It dilutes blood samples, runs a buffer gradient to increase ionic strength, and measures separated hemoglobin passing through a flow cell at 415 nm. Whole blood must be collected in EDTA tubes and can be stored for up to 7 days at 2-8 degrees Celsius. Reagents must be inspected for discoloration, cloudiness, precipitation, or leakage and discarded if issues are observed.
The BioRad D10 uses high performance liquid chromatography to separate hemoglobin in whole blood samples into types based on ionic interactions. It dilutes blood samples, runs a buffer gradient to increase ionic strength, and measures separated hemoglobin passing through a flow cell at 415 nm. Whole blood must be collected in EDTA tubes and can be stored for up to 7 days at 2-8 degrees Celsius. Reagents must be inspected for discoloration, cloudiness, precipitation, or leakage and discarded if issues are observed.
The BioRad D10 uses high performance liquid chromatography to separate hemoglobin in whole blood samples into types based on ionic interactions. It dilutes blood samples, runs a buffer gradient to increase ionic strength, and measures separated hemoglobin passing through a flow cell at 415 nm. Whole blood must be collected in EDTA tubes and can be stored for up to 7 days at 2-8 degrees Celsius. Reagents must be inspected for discoloration, cloudiness, precipitation, or leakage and discarded if issues are observed.
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BioRad D10
Specimen Specimen Handling Buffer Handling Whole Blood Handling Method Selection Calibration Limitations Limitations Precision Accuracy Recovery Mechanism • D-10 Hemoglobin A1C program utilizes principles of ion-exchange high performance liquid chromatography (HPLC) • Sample diluted on the D10 then injected into analytical cartridge • D10 deliver programmed buffer gradient to increase ionic strength to the cartridge • Hemoglobin separated based on their ionic interaction with cartridge material • Separated Hb pass through flow cell of filter photometer then measured in the absorbance at 415 nm Specimen • The specimen is whole blood • Collected in a vacuum collection tube containing EDTA • WB specimens may be stored up to 7 days at 2-8 celcius degree Instability of Reagens • If reagents were frozen during shipment, mix each bottle by gently inverting before installing on instrument. • Do not use any reagents which have any indications of discoloration, cloudiness or precipitation. • Do not use any reagents that show any signs of leakage. • Do not use the calibrator or whole blood primer if the pellet is brown or the vial is broken. If the lyophilized material contains insoluble matter, discard the material and reconstitute a new vial.
