Scribd Logo
Upload

Welcome to Scribd!

  • 63 views

    Column Chromatography

    Uploaded by

    Criminegrology Tv
    Column chromatography is a technique used to separate mixtures by passing a solution through a column packed with a solid stationary phase. The individual components of the mixture move through the column at different rates depending on how strongly they interact with the stationary phase, allowing separation. Key aspects of column chromatography include using a glass or plastic tube as the column, packing it with an absorbent like silica or alumina as the stationary phase, and passing a liquid or mixture of liquids as the mobile phase to carry the sample through the column. It is a widely used but time-consuming chromatography method.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    Column Chromatography

    Uploaded by

    Criminegrology Tv
    63 views9 pages
    Column chromatography is a technique used to separate mixtures by passing a solution through a column packed with a solid stationary phase. The individual components of the mixture move through the column at different rates depending on how strongly they interact with the stationary phase, allowing separation. Key aspects of column chromatography include using a glass or plastic tube as the column, packing it with an absorbent like silica or alumina as the stationary phase, and passing a liquid or mixture of liquids as the mobile phase to carry the sample through the column. It is a widely used but time-consuming chromatography method.

    Original Description:

    CC

    Original Title

    COLUMN-CHROMATOGRAPHY

    Copyright

    © © All Rights Reserved

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Column chromatography is a technique used to separate mixtures by passing a solution through a column packed with a solid stationary phase. The individual components of the mixture move through the column at different rates depending on how strongly they interact with the stationary phase, allowing separation. Key aspects of column chromatography include using a glass or plastic tube as the column, packing it with an absorbent like silica or alumina as the stationary phase, and passing a liquid or mixture of liquids as the mobile phase to carry the sample through the column. It is a widely used but time-consuming chromatography method.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    63 views9 pages

    Column Chromatography

    Uploaded by

    Criminegrology Tv
    Column chromatography is a technique used to separate mixtures by passing a solution through a column packed with a solid stationary phase. The individual components of the mixture move through the column at different rates depending on how strongly they interact with the stationary phase, allowing separation. Key aspects of column chromatography include using a glass or plastic tube as the column, packing it with an absorbent like silica or alumina as the stationary phase, and passing a liquid or mixture of liquids as the mobile phase to carry the sample through the column. It is a widely used but time-consuming chromatography method.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    You are on page 1of 9

    COLUMN

    CHROMATOGRAPHY
    GROUP 1
    Agbayani, Mark Kevil
    Ahuyan, Eugine P.
    Bojno, Nadia B.
    Bulayang, Hanz Christian A.
    Capua, Russel A. Jr.
    INTRODUCTION

     Chromatography is purification technique. Individual components


    are separated from a mixture by passing it in solution or suspension
    through a medium in which the components move at different rates.
    In case of column chromatography the separation occurs with help
    of a column, therefore it is termed as column chromatography.
     Column is a tube like structure made of glass or plastic.
    PRINCIPLE

     The main principle involve is absorption of solutes through a


    stationary phase and separates the mixture into individual
    components.
     The mostly used stationary phases are salica and alumina. Mobile
    phase may be single phase solvent or mixture mobile phase is
    opposite in nature to stationary phase.
    COLUMN CHROMATOGRAPHY
     It is a most widely used chromatography which finds daily use in
    research labs and industries. It is easy to operate and less expensive
    technique. As the name indicates, there is a long column which is
    suspended in the air with the help of stand.
     Into the column, the stationary phase is the packed and the mobile
    phase is allowed to flow taking the sample through the solid
    column.
    INSTRUMENTATION OF COLUMN
    CHROMATOGRAPHY
     Mobile Phase - Liquid
     Stationary Phase - Solid
    PREPARATION OF COLUMN
     The column mostly made of glass tube packed with stationary phase
    either salica or alumina.
     A glass woll or cotton is placed at the bottom of the column before
    packing the stationary phase.
    Two types of preparing the column:
     Dry Packing or Dry Filling
    - The Dry Powder is filled in the column and then the solvent is
    allowed to flow through the column.
     Wet Packing
    - The slurry of absorbent with the mobile phase is prepared and is
    poured into the column stationary phase should be filled uniformly
    without any air bubbles.
    ADVANTAGES:
     Any type of mixture can be separated by the Column
    Chromatography.
     Wider choice of mobile phase.
     No limit of quantity as any amount of mixture can be separated by
    this technique.
     It is a robust method.
     The separated analytes can be reused.
    DISADVANTAGES:
     It is time-consuming process for the separation of compounds.
     It is expensive as higher quantities of solvents are required.
     The automated process becomes complicated and therefore costly.
     It has a low separation power.

    You might also like