Light Microscope

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LIGHT

MICROSCOPE

BY
ANKIT MISHRA
ROLL NO - 09
WHAT IS A LIGHT MICROSCOPE ?
Light microscopy refers to the use of any kind of microscope that
uses visible light to observe specimens.
A light microscope is a biology laboratory instrument or tool,
that uses visible light to detect and magnify very small objects
and enlarge them.
It is so-called because it employs the use of white or visible light
to illuminate the object of interest so it can be magnified and
viewed through one or a series of lenses.
•They use lenses to focus light on the specimen, magnifying it thus
producing an image. The specimen is normally placed close to the
microscopic lens.
•Microscopic magnification varies greatly depending on the types
and series of lenses that make up the microscope. Depending on the
number of lenses, there are two types of microscopes i. e Simple
light microscope (it has low magnification because it uses a single
lens) and the Compound light microscope (it has a higher
magnification compared to the simple microscope because it uses
at least two sets of lenses, an objective lens, and an eyepiece).
•The lenses are aligned in that, they can be able to bend light for
efficient magnification of the image.
TYPE OF MICROSCOPE

The modern types of Light Microscopes include:


1. Brightfield Light Microscope (Compound light microscope)
2. Phase Contrast Microscope
3. Dark-Field Light Microscope
4. Fluorescent Microscope
Parts of
Light
Microscope
Parts of a Compound Microscope
Eyepiece And Body Tube. Illuminator and Stage
 The eyepiece is the lens through which the viewer looks to  The illuminator is the light source for a microscope.
see the specimen.  A compound light microscope mostly uses a low voltage bulb as an
 It usually contains a 10X or 15X power lens. illuminator.
 The body tube connects the eyepiece to the objective lenses.  The stage is the flat platform where the slide is placed.

Objectives and Stage Clips Nosepiece and Aperture


 Objective Lenses are one of the most important parts of a  Nosepiece is a rotating turret that holds the objective lenses.
Compound Microscope.  The viewer spins the nosepiece to select different objective lenses.
 They are the closest to the specimen.  The aperture is the middle of the stage that allows light from the
 A standard Microscope has three to four Objective Lenses illuminator to reach the specimen.
which range from 10X,40 X to 100X.
 Stage Clips are metal clips that held the slide in a place.
Condenser, Iris diaphragm, and Diaphragm
 A condenser gathers and focuses light from the illuminator onto the
Arm and Base specimen being viewed.
 The Arm connects the Body Tube to the base of the  Iris diaphragm adjusts the amount of light that reaches the specimen.
Microscope.  The diaphragm is a five holed disk placed under the stage.
 The Base supports the Microscope and its where Illuminator.  Each hole is of a different diameter. By turning it, you can vary the
amount of light passing through the stage opening.
MAGNIFCATION
TITLE LOREM IPSUM

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