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    Lecture 17 - Slides

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    ash.smithy0624
    The document discusses midterm grades for a class, noting that assignments are due next week and the final exam will be on June 21st from 9am to 12pm in room HL170. It also provides information on the cytoskeleton, including the main components of actin filaments, microtubules, and intermediate filaments, and how they help provide structure, shape, and movement within cells. Dynamic instability allows microtubules to quickly change between growing and shrinking states in response to cellular needs.

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    Lecture 17 - Slides

    Uploaded by

    ash.smithy0624
    21 views37 pages
    The document discusses midterm grades for a class, noting that assignments are due next week and the final exam will be on June 21st from 9am to 12pm in room HL170. It also provides information on the cytoskeleton, including the main components of actin filaments, microtubules, and intermediate filaments, and how they help provide structure, shape, and movement within cells. Dynamic instability allows microtubules to quickly change between growing and shrinking states in response to cellular needs.

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    The document discusses midterm grades for a class, noting that assignments are due next week and the final exam will be on June 21st from 9am to 12pm in room HL170. It also provides information on the cytoskeleton, including the main components of actin filaments, microtubules, and intermediate filaments, and how they help provide structure, shape, and movement within cells. Dynamic instability allows microtubules to quickly change between growing and shrinking states in response to cellular needs.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    21 views37 pages

    Lecture 17 - Slides

    Uploaded by

    ash.smithy0624
    The document discusses midterm grades for a class, noting that assignments are due next week and the final exam will be on June 21st from 9am to 12pm in room HL170. It also provides information on the cytoskeleton, including the main components of actin filaments, microtubules, and intermediate filaments, and how they help provide structure, shape, and movement within cells. Dynamic instability allows microtubules to quickly change between growing and shrinking states in response to cellular needs.

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    Midterm Grades

    Assignments – due next week!

    Final Exam: June 21st 9-12pm, HL170


    The Cytoskeleton
    The Cytoskeleton
    System of filaments

    Actin

    Microtubules
    Actin Filaments

    Underlie the plasma membrane – shape cell surface

    Provide strength and shape

    Movement

    Muscle contractions
    Microtubules

    Extend to cell periphery

    Can quickly rearrange themselves to form mitotic spindle


    during cell division

    Can serve as tracks – help determine position of


    membrane-bound organelles
    Intermediate Filaments

    Provides strength

    Line the inner face of the nuclear envelope 


    protective cage for the cell’s DNA

    Allow the formation of tough appendages


    Cytoskeletal Filament Systems

    Dynamic and adaptable

    Regulation of the dynamic behaviour and assembly of


    cytoskeletal filaments allow euk. cells to build many
    structures
    Microtubules’ role during cell division

    McGraw Hills Animation, 2017


    The Contractile Ring
    Actin’s role during cell division

    McGraw Hills Animation, 2017


    Crawling Cell
    Crawling Cell
    Cell – cell
    interactions
    Filaments assemble from protein subunits
    Subunits

    1. Actin Filaments – actin subunits [G-actin]

    2. Microtubules – tubulin subunits

    3. Intermediate Filaments - fibrous subunits that


    are elongated
    Subunits
    1. Actin Filaments – actin subunits – ATP

    2. Microtubules – tubulin subunits – GTP

    3. Intermediate Filaments - fibrous subunits that are


    elongated
    Hydrolysis
    A G

    GTP GDP
    Accessory Proteins and Motors
    Cell regulates
    -length
    -stability
    -number
    -geometry
    of its cytoskeletal filaments

    Work with the cytoskeletal filaments and allow them to


    carry out their roles in the cell
    Microtubules & Motors
    Tubulin Subunit:

    1 Protofilament:


    13 Protofilaments = Microtubule


    Microtubule’s subunit: Tubulin
    +End
    - End

    Subunit = Tubulin
    - a heterodimer +End

    --tubulin &
    - -tubulin - End
    Microtubule

    + End

    Made of 13 parallel protofilaments

    Each composed of  & -tubulin

    Additional lateral contacts

    - End
    Kon –rate of addition of subunits

    Koff – rate of removal of subunits


    Tubulin can hydrolyze GTP
    Tubulin Subunits

    T form – GTP bound

    D form –GDP bound


    Hydrolysis of the bound nucleotide reduces the binding
    affinity of the subunit for neighboring subunits
    Rate of addition of subunits can be faster than rate at which
    they are hydrolyzed
    Dynamic Instability
    Rate of GTP hydrolysis:
    Low:

    High?
    Similar as subunit addition?
    New subunit is added Hydrolysis occurs
    before previously added before the next
    subunit is hydrolyzed subunit is added

    Tip: T-Form Tip: D-Form


    Dynamic Instability
    Catastrophe: Growth  Shrinkage

    Rescue: Shrinkage  Growth


    Dynamic Instability
    Think Pair Share
    Dynamic Instability
    Dynamic Instability
    Critical Concentration [Cc]

    Kon = Koff
    Course of Polymerization

    Steady State phase

    Nucleation
    Break

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