2A3 Osmosis

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2A- 3 Osmosis

TO UNDERSTAND WHAT IS MEANT BY OSMOSIS IN TERMS OF MOVEMENT OF FREE


WATER MOLECULES THROUGH A PARTIALLY PERMEABLE MEMBRANE, DOWN A
WATER POTENTIAL GRADIENT
What is Osmosis ?

 Osmosis is the net movement of free water molecules through a partially


permeable membrane, down a water potential gradient.
 Water potential of a solution measures the concentration of free water molecules-
the water molecules that are not associated with solute molecules. More free water
molecules mean a higher water potential.
 Therefore, osmosis in cells involves the movement of water from a region of
high-water potential to a region of lower water potential down a water potential
gradient across a partially permeable membrane such as the cell surface
membrane or nuclear membrane.
The osmotic
concentration
 The osmotic concentration of a
solution concern only those solutes
that have an osmotic effect.
 Many large insoluble molecules found
in cytoplasm(starch and lipids), do not
affect the movement of water and so
are ignored when considering osmotic
concentration. Only soluble particle
are considered (including plasma
proteins such as albumin and
fibrinogen).
Modelling
osmosis in
cell;
Sucrose
example
 Recap;

In animals
 (A) In hypotonic solution, water
moves in and the cell swells and
burst
 (B) In Isotonic solution the red
blood cell maintain its normal
shape
 (C) In Hypertonic solution, water
moves out and the cell shrivels
In plants
• In plant cells, the cellulose cell wall prevents cells bursting. If the surrounding fluid
is hypotonic to the cytoplasm of a plant cell water will enter the cell by osmosis but
not indefinitely.
• This is because cytoplasm swells and presses on the cell walls, it generates hydrostatic
pressure.
• The inward pressure of the cell wall on the cytoplasm increases until it cancels out the
tendency of water molecules to move in.
• This inward pressure is called pressure potential.
• When the osmotic force moving water into plant cell is balanced by the pressure
potential forcing it out, the plant cell is rigid, in a state known as turgor.
In plants

 When the surrounding solution is hypertonic, water moves out of the cell by
osmosis and turgor is lost. The cell membrane begins to pull away from the cell
wall as the protoplasm (cytoplasm+nucleus) shrinks. This is called incipient
plasmolysis.
 We measure incipient plasmolysis using serial dilations, looking for the point at
which 50% of the cells are plasmolyzed and 50% are not.
 If the cell in a hypertonic solution water will move out, vacuole will be reduced,
and the protoplasm will shrink away completely- the cell suffers from
plasmolysis.
 However, the presence of cell wall causes no/little change in the size and shape of
the plant( whether they are fully turgid or plasmolyzed). They do not swell and
burst, nor do they become very small. Only the contents change.
Example of red beet plant
TASK

Check out the summary of Osmosis and water potential video;


https://www.youtube.com/watch?v=L-osEc07vMs

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