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CONFIRMATION OF MSCs by

FLOW CYTOMETRY

Presented by
Surbhi
What are Mesenchymal Stem Cells (MSCs)

 Mesenchymal stem cells (MSCs) are adult stem cells isolated from different

sources that can differentiate into other types of cells.

 In humans, these sources are bone marrow, fat (adipose tissue), umbilical

cord tissue (Wharton’s Jelly) or amniotic fluid (the fluid surrounding a fetus).

 Wharton’s Jelly is the gelatinous connective tissue that is present inside

umbilical cord.

 MSCs, can differentiate into many different types of cells within the body,

Bone cells, Cartilage, Muscle cells, Neural cells, Skin cells, and Corneal cells.
Differentiation of MSCs
FLOW CYTOMETRY FOR MSC
IDENTIFICATION, VERIFICATION

 Reveals the phenotype of individual cells


 • Examines several markers simultaneously
 • Can be used to isolate specific populations (when equipped with a cell sorter)
 • High sensitivity
 • High speed analysis
 • Can use gating to remove dead cells from analysis

BD FACS CALIBUR
Selection of marker for MSCs
 The International Society for Cellular Therapy {ISCT} proposed positive and
negative markers that enabled researchers to distinguish MSCs from other
cells in the bone marrow compartment.

 The negative markers were selected to include surface antigens that are
expressed by hematopoietic cells, while

 the positive markers were selected to include surface antigens that are
absent from most hematopoietic cells

 The purity of MSCs can be increased by using more than one MSC marker for
positive selection.
Combination of Positive marker and
Negative marker
Graphical Representation of MSCs
CONCLUSION

 Wharton’s Jelly-derived Mesenchymal stem cells (WJ-MSCs) have been


found to be an alternative source of stem cells for the regenerative
medicine. This is as WJ-MSCs have potential for differentiation, self-
renewal and unique immunomodulatory properties.

 Flow cytometry is the gold standard clinical tool for analyzing


expression of cell surface markers and evaluating the cell population
composition in a sample.

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