WQLTA
WQLTA
WQLTA
February, 2023
Mekelle, Tigray, Ethiopia
10/31/24 WQMHHLT 1
Training duration
• February, 23-25.2023
Title
• Water quality laboratory,
Analysis,
Test and
Treatment
Sanitary survey
Others
10/31/24 WQLTA 2
H2O
10/31/24 WQLTA 3
Introductory Sessions
Building Relationship
Introduction
–Facilitators Introduction
–Participants Introduction
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Establishing Ground Rule
10/31/24 WQLTA 5
Cont'd...
Pa Participants’
s’ rtic
i pa
nt
Pr
e
–te Expectation
en as st
po try &
br ints Fear
ai
ns an 8 mnts
15 to d
rm
m
nt
s
10/31/24 WQLTA 6
Training contents
• Hygiene and Sanitation over view
• Bacteriological and analysis
• Chemical and analysis
• Physico-chemical and analysis
• Sanitary survey
• Sampling methods
• Practical session
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Methodology...
• Theoretical presentation = LCD
• Questions and Answers
• Big group discussion and presentation = LCD
• Practical demonstration = at field and lab room
Sampling
Analysis
Interpretation
Recommendation
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Objectives of the ToT
• Participants build common/shared understanding on WQ parameters
• To facilitate the establishment of system which creates safe,
accessible, affordable and sustainable water supply in the area.
10/31/24 WQ 9
Project overall objective
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Project purpose
• To capacitate the staff of all Partner’s in Concern World wide
Ethiopia sub office Tigray and the staff of the local government and
stakholders;
• To make the local populations aware of water quality issues.
Safe dirinking water supply
1. Safe and meet the STD
Free from pathogenic microorganisms
Free from physical foreign bodies
Chemical parametres = Fulfill the standard
2. Access = for all with in STD (amount) 25l/c/d
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Water ===> Life
• Functions of Water in the body...
Water is essential for physiological processes of digestion, absorption
and excretion.
It plays a key role in the structure and function of the circulatory system
and acts as transport medium for nutrients and all body substances.
Water plays a direct role in maintaining body temperature.
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Cont'd...
The average adult human body is 50-65% water,
Averaging around 57-60%.
The percentage of water in infants is much higher, typically around 75-
78% water, dropping to 65% by one year of age.
Body composition varies according to gender and fitness level because
fatty tissue contains less water than lean tissue.
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Cont'd...
Water promotes
The absorption of nutrients,
Carries nutrients to cells,
Dissolves and eliminates excess products of metabolism,
Moistens the air we breathe,
It helps regulate body temperature through evaporation of
perspiration,
Facilitates joint flexibility, and
Creates a flexible protective coating around our vital ...
10/31/24 WQLTA 15
Cont'd...
• What are the vital functions of water in my body?
Helps your kidneys flush out all that waste.
Your total is how many ounces of water you should drink each
day.
Divide that number by 8 to see your result in cups.
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Cont'd…
According WHO std
1. Safe water supply should be:-
• Amount =30-40 l/c/d/
(excluding other domestic uses)
• Distance = 15-20 mnts W.I 1$
• Access = to ALL
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Introduction
• Water is one of the indispensable natural resources
required for the existence of all living things. In
quantity and quality.
• Water in nature
a, Colorless
b, Odorless
c, Tasteless
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Introduction to Drinking Water
10/31/24 WQLTA 21
Cont'd...
• Having safe drinking water and basic sanitation is a human
need and right for every man, woman and child.
10/31/24 WQLTA 22
Water quality
• Water quality is the physical, chemical and biological
characteristics of properties of water that determine the
fitness for use of a specific water source
10/31/24 WQLTA 23
Group discusion-2
The difference b/n pollution and contamination
10/31/24 WQLTA 25
Cont'd...
• Pollution is after the end process,
pollution happens to nature, soil, water, air, light, etc.
The waste (products that are waste in the process) reacts with the
surroundings they are dumped at.
Substance (physical, chemical or biological).
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Water Pollution
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Point Source Pollution
comes from a specific source,
like a pipe
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Nonpoint source
Nonpoint Source (NPS) Pollution
is pollution associated with
stormwater or runoff
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Sanitary survey
• The evaluation of the water source and intake structure, the treatment
and conditioning process, the facilities and components and also an
evaluation of the distribution system
• Inspections
• Types of the water points
• Location
• Possible contamination for the water points
• Structure of the water points
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Free from
Bacteria
Radioactive
Organic
substances
element
absent absent
Safe Water/
wholesome
Water
Chemically Aesthetically
safe acceptable
WQLTA
10/31/24 31
Cont'd…
2, Chemical/mineral elements
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CLEAR
PURE
WATER ?
SAFE
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3. WATER QUALITY
CHARACTERISTICS
Potable water quality performs the following basic
requirements.
It should be free from bacteria that causes disease;
Free from compounds that cause an offensive taste
& odor;
It should be colorless/clear;
It should not corrode pipes;
It should be free from all objectionable matters;
It should be not saline;
low in concentrations of compounds are acutely or
toxic;
10/31/24 TWRB, Water Quality Control 35
Team
Cont'd…
10/31/24 WQLTA 36
Water Quality parameters
1. Microbiological:
3. Chemical:
• Faecal coliforms
Arsenic
• Total coliform
Fluoride
2. Physical:
Nitrate
• pH
• Temperature Nitrite
• Turbidity Iron
• Taste Manganese
• Odur Total dissolved solids /
• color
conductivity
• 10/31/24
Appearance WQLTA
37
10/31/24 WQLTA 38
Bacteriological analysis :
mainly includes estimation of
Total coliforms(37d.cel in 18 - 24hrs)
Fecal coliform(thermo tolerant 44 degree Celsius
in 18 - 24hrs)
• Drying
• Temperature
• pH
• uV Radiation
• Competition
• Predation
• Toxic substance
10/31/24 WQLTA 41
Microbial…
• The common MP are:
1, Coliform Groups
a, Total coliform
c, Escherichia coli
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Membrane Filter Methods
• Filter water through a 0.45 μM membrane
filter
• Place membrane on selective media
• Incubate
– 35 C total coliform for 18hr Vs 48hr
– 44.5 C fecal coliform 18hr vs 24hr
• Count colonies with necked eye/glace x4
• Count only the yellowish color only.
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International & USAID water quality guideline for selected
constituents (Maximum allowable)
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WHO (1993), Guideline for water quality
Coliform Group
• Total coliform
• Fecal coliform
– All total coliform criteria total coliform
– Grows at 44.5 C
fecal
• Escherichia coli coliform
– Individual species
– Enzyme specific E. coli
10/31/24 WQLTA 46
Cont'd...
10/31/24 WQLTA 47
Counting of colonies
count only
yellow color
coloni
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Bacteriological
10/31/24 WQLTA 49
10/31/24 WQLTA 50
Cont'd…
• Household water sources:-
can come from Three main source
1, Municipality
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Cont'd...
2, Ground water (needs special follow up and
inspections)
10/31/24 WQLTA 53
Cont'd...
Improved drinking-water sources:
– Piped water into dwelling, yard or plot
– Public tap or standpipe
– Tubewell or borehole
– Protected dug well
– Protected spring
– Rainwater collection
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Cont'd…
• unprotected spring
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Cont'd...
• Sometimes the contamination is not obvious such as it is:-
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Important Note:-
• Microbiological contamination of drinking water is the greatest
concern to human health in most developing countries.
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General consideration of WHO
• Water is essential to sustain to life. And a satisfactory
(adequate, safe, and accessible)
• Supply must be available to all.
• Improving access to safe drinking-water can result in
tangible benefits to health.
• Every effort should be made to achieve a drinking-water
quality as safe as practicable.
10/31/24 WQLTA 59
Cont'd…
According WHO std
1. Safe water supply should be:-
• Amount = 30-40 l/c/d
(excluding other domestic uses)
• Distance = 15-20 mnts
• Access = to ALL
10/31/24 WQLTA 60
Cont'd...
Microbial aspects ( WQ WHO guidelines)
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Cont'd...
10/31/24 WQLTA 62
Cont'd...
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Water is contaminated
• At source
• During transportation pipe, container, etc
• At home (containers)
• During preparation and consumptions
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Possible ways to contaminant water points
Human made
Open defecation
Wastes for different plants
Domestic waste
Municipality wastes
Vehicle wastes
Pesticides and herbicides
Animal wastes
Natural phenomenon
Others
Point and none point sources
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Importance of safe Water
Cont'd...
•Essential for the prevention of disease, especially in developing nations
o Reduce the number of insect-related disease,
disease such as malaria, as well
as air-borne diseases
o Improving individual health
o Improve Sanitation
• Basic needs
o Bathing
o Drinking
o Cooking
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Students Without Water
• UNICEF estimates over 50% of world’s
schools have no access to drinking
water/sanitation
• Safe water leads to fewer water-borne
diseases, which means fewer missed
classes
• Children (esp. girls) often miss class to
fetch water
Prone to drop out…..less performance
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Water contaminated through
• Human (open defecation continued= is vital)
a, poor sanitation and hygiene environments
• Animal (dugs)
• Farmlands (pesticides, herbicides, urea etc…)
• Points and non points sources
a, know and unknown sources
• Factories (small and large scale industries)
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Water associated diseases
• WHO recognizes that access to adequate water supplies is a
fundamental human right.
10/31/24 WQLTA 69
Cont'd...
• Each person needs 20-50 liters of safe freshwater a day
for drinking, cooking and cleaning.
o
More than one/six people worldwide -
10/31/24 WQLTA 70
Cont'd...
By 2025, 800 million people will experience absolute water scarcity, and
two-thirds of the world population could be under stress conditions.
1.1 billion people have no access to any type of improved drinking
source of water.
10/31/24 WQLTA 71
Cont'd...
……. In comparison …..
• On average, people in Europe use more than 200 liters—
in the United States more than 400 liters.
• The 1.1 billion people who don’t have access to drinking
water only use up to 5 liters- one-tenth of the average
daily amount used in rich countries to flush toilets.
10/31/24 WQLTA 72
Consequences…
Think Guinea worm and
Related
• At the start of the 21st century un-safe water is the world’s second
biggest killer of children.
• 1.6 million people die every year from diarrhoeal diseases, the leading
cause of disease worldwide
o 90% of these are children under 5
• 133 million suffer from high intensity intestinal helminthes (parasitic
worms)
worms infections
o 1.5 million cases of clinical hepatitis
10/31/24 WQLTA A every year 73
Water associated…
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Water associated…
Disease Transmission
A, Many diseases may be transmitted via the fecal-oral
route ,and that occurs when human faecal is ingested
through drinking contaminated water or eating
contaminated food .
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Contamination....
• One GRAM of faeces contains:
10,000,000 viruses
1,000,000 bacteria
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10/31/24 WQLTA 77
Cont'd...
k i l l e r
e l e s s
v oi c
h i t i s
S
10/31/24 WQLTA 78
F-diagram Transmi. And barrier…
Outcome
Flies
. Healthful
individual
Shits Fingers
Solid and Water Future
Food Host
Liquid
wastes
Mortality
Fluids morbidity
• Water-borne diseases .
• Water-based diseases.
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10/31/24 WQLTA 83
Water quality analysis – key factors
Sampling Selection of
Parameters
Preservation
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Types of sampling…
Surface water
Grab sampling
Composite sampling
Integrated
Ground water (well, open BH)
Tap water(hand pump, pipe line/distribution point
10/31/24 WQLTA 85
Cont'd...
• Surface water
Types of Samples
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Steps and materials…
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Sample Collection and storage tips
1. Sampling Strategies;
Types of Wpts, Number and types of sample, Location, Depths, date
and times, woreda, Tabia, Kushet, name of Wpts,
2. Sapling Methods;
Types of Sample, Techniques, equipment's =(based on the purpose)
3. Sample Storage;
Types of container, Preservative Mtds, Maximum holding
times = according Sample purpose
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STANDARDS - SAMPLING:
The number of bacteriological samples to be taken is based on the
following:
SN Water supply system per population Number of sample per month
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Cont'd...
Are pointed upwards
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Sampling…
Aims of Sampling
• Quality Consistency
• Representative Sample
• Prevent Deterioration
• Prevent Contamination
• Reliable Results
• Suitable Sampling Techniques
• Correct Sampling Bottles
• Preservatives
• Accurate Field Measurements
• Transportation
10/31/24
• Time WQLTA 92
Cont'd...
Field Sheets
•Reminder of where and what need to
sampled/measured
•Permanent record of sample details
•Record of field measurements
•Observations
•Deviations
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Cont'd...
•Sampling location (name and/or code)
•Sampling date and time
• Name & signature of sample collector
• Results of field measurements e.g. Temp, DO, pH
• Types of samples taken (general chemical, metals, etc.)
Details of any preservative used/ filtration carried out
• GPS readings – if new location or non-routine site
•Observations – weather, visual inspection
•Deviations
10/31/24 WQLTA 94
Treatment ?
10/31/24 WQLTA 95
Treatment...
• Proper treatment and handling of drinking
water is essential not only for the quality of
water but also for human health.
10/31/24 WQLTA 96
Treatment
• House hold level
• School
• Municipality/community
10/31/24 WQLTA 97
House hold level Water treatment
chemical Methodelogy of disinifection
5 ዝሩግ ማይ እንተኾይኑ ናብ 20-25ሊ ዕፅፊ ብምግባር በቶም ካብ 1-4 ዝተቐመጡ ዓይነት ቅመም
ን5 ደቒቓ ብምሕቓቕን ዝግ ብምባል ድሕሪ 30 ደቒቓ ኣብ ጥቕሚ ክውዕል ይኽእል፡፡
10/31/24 WQLTA 98
Cont'd...
• In water distribution centers, 4 different
methods are used to treat drinking water:
Boiling
SODIS
Flocculation,
Sedimentation,
Filtration, and
Disinfection:
10/31/24 WQLTA 99
Group discusion t s
m n
l 2 5
Two Big
Tot a
Groups
• Hygiene
The concept and the difference b/n
• Sanitation
• Water quality
• The hygiene and sanitation domains
• The three key hygiene and sanitations
• Basic sanitation
• Onsite Sanitation
• Offsite Sanitation
• Improved Sanitation
Ethic Skilled
al Optimist
vs
Having an Attiude Pesimist