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Differential Leukocyte Count

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Umm e Aiman
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20 views23 pages

Differential Leukocyte Count

Uploaded by

Umm e Aiman
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPT, PDF, TXT or read online on Scribd
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Dr.

Farhan Ahmed
Consultant Hematologist
LEARNING OBJECTIVES
 At the end of lecture students will able to know

1. Types of WBC.
2. Maturation
3. Function.
4. Diseases
White Blood Cells

 Leukocytes include myeloid cells and lymphoid


cells.

 Myeloid cells consist of neutrophils, eosinophils,


basophils and monocytes.

 Lymphoidcells include small and large


lymphocytes and natural killer (NK) cells.
Maturation
 Granulocytic precursor do not normally appears in
the blood but they are found in the bone-marrow.
 The earlier form granulocytic precursor
 Myeloblast.
 Promyelocyte.
 Myelocyte.
 Metamyelocyte.
 Neutrophils.
Time to make maturation of neutrophils:
Maturation From 0 hour to 300 hours.
MYELOBLAST
 Characterized by variable in
sized that has large nucleus
with fine chromatin and
usually two to five nucleoli.
 Cytoplasm of myeloblast is
basophilic and no granules
seen in the cytoplasm.
 Normally myeloblast in the
bone marrow is < 5%.
PROMYELOCYTES

 Which are slightly


larger in sized and
develop primary
granules / Azurophilic
granules in the
cytoplasm.
Myelocytes

 Which are condensed


nuclear chromatin and
contain primary and
secondary granules.
 Note: sunrise effect
seen in myelocytes.
Metamyelocytes

 Itis differ from


myelocyte by slightly
indentation of nucleus.

 Nuclear indentation <


half.
Band Cells

 Nuclear indentation > half


NEUTROPHILS

 Neutrophils are also called


polymorph.
 These cells are
characterized by dense
nucleus having 2-5 lobes.
 Pale cytoplasm with uneven
outlines containing many
fine pink blue azurophilic
grains.
LYMPHOCYTES

Small Large granular


Lymphocytes Lymphocytes
Small lymphocytes

 Largerthan RBCs.
 Condensed nuclear
chromatin.
 Rim of cytoplasm.
 Nucleoli are rarely
seen.
Large Granular Lymphocytes
 Cytoplasm of LGL has
lighter stained that of
small lymphocytes.

 Azurophilic granules
are seen and the
nucleus is more
variable in shape and
often eccentric.
Eosinophils
 Eosinophils arise from the same stem cell
 Earliest point at which eosinophils can be
morphologically defined in the bone marrow is at the
promyelocyte stage.
 Promyelocytes contain large granules stain blue–red
 Metamyelocyte stage golden-red granules filling the
cytoplasm
 Mature eosinophils usually have only two segments
Basophils

 Earlieststage at which they can be identified is the


promyelocyte stage at which large, black–violet
stained granules are visible.
 Mature basophils, which are relatively small, two
compact nuclear segments like blackberries.
Monocytes

 Arise from bone


marrow.
 Earliest stage is
monoblast then
promonocytes and
mature monocytes.
 Measuring anywhere
between 20 and 40 μm
in size.
Terminologies used to describe
variation in the WBC count

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