Sanger sequencing

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Sanger Sequencing, Dna Sequence, Ground Breaking, Medical Science, Light Sensor, The Gold, The Basics, Lab, Bench

Let’s go back to the basics and explore the technology platform that has been regarded as the gold standard for many years. You guessed it – we’re talking about Sanger Sequencing by capillary electrophoresis. Many might ask, “why is it called Sanger Sequencing?” Sanger Sequencing is named after the inventor of this ground breaking technology, Dr. Frederick Sanger, who

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Sanger Sequencing, Dna Sequencing, Dna Facts, Bio Notes, Dna Polymerase, Next Generation Sequencing, Genome Sequencing, Color Knowledge, Dna Replication

Determining the order of the nucleotides present in the DNA is called a DNA sequencing. Our DNA is made up of the 4 nitrogenous bases adenine, thymine, cytosine and guanine. A long chain of the bases which codes for a particular protein is called as a gene.

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RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing- and microarray-based methods, RNA-Seq provides far higher coverage and greater resolution of the dynamic nature of the transcriptome. Beyond quantifying gene expression, the data generated by RNA-Seq facilitate the discovery of novel transcripts, identification of alternatively spliced genes, and detection of allele-spec Sanger Sequencing, Laboratory Techniques, Experimental Design, Gene Expression, Molecular Biology, A Cell, The Cell, Transcription, Data Analysis

RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing- and microarray-based methods, RNA-Seq provides far higher coverage and greater resolution of the dynamic nature of the transcriptome. Beyond quantifying gene expression, the data generated by RNA-Seq facilitate the discovery of novel transcripts, identification of alternatively spliced genes, and detection of…

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