Flow cytometry analysis

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Label-free cell cycle analysis for high-throughput imaging flow cytometry | Nature Communications Supervised Machine Learning, Cycle Phases, Fluorescence Microscopy, Flow Cytometry, Cell Cycle, Free Labels, Machine Learning, How To Apply, Nature

Imaging flow cytometry enables high-throughput acquisition of fluorescence, brightfield and darkfield images of biological cells. Here, Blasi et al.demonstrate that applying machine learning algorithms on brightfield and darkfield images can detect cellular phenotypes without the need for fluorescent stains, enabling label-free assays.

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Basic Protein Characterization: Profacgen’s biochemical research facility enables the comprehensive analysis of basic protein characteristics. Various technologies, including chromatography, electrophoresis, immuno blotting, immune-precipitation, flow-cytometry, microscopy, are used in our facility for protein identification, separation, and analysis. Flow Cytometry Analysis, Oxidation State, Flow Cytometry, College Paper, Research Facility, Lab Technician, Cell Biology, Scientific Research, Biology

Profacgen provides comprehensive protein characterization services, including protein identification, expression profile analysis, purity test, oxidation status test, aggregation status test etc., to facilitate your scientific research. Our service can be tailored according to your specific requirements.

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This is done by flow cytometric analysis for detection of lack of GpIb/IX in Bernanrd Soulier syndrome (deficiency of CD42), and lack of GpIIb/IIIa in Glanzmann’s thrombasthenia (deficiency of CD41, CD61).
 
What is the best protocol for platelet glycoprotein (GPIIb/IIIa) analysis using flow cytometry?
 

Fresh platelets should always be used. Storing platelets dramatically changes the level of transmembrane proteins. The best way is to follow one of standardized protocols defined Flow Cytometry, Always Be

This is done by flow cytometric analysis for detection of lack of GpIb/IX in Bernanrd Soulier syndrome (deficiency of CD42), and lack of GpIIb/IIIa in Glanzmann’s thrombasthenia (deficiency of CD41, CD61). What is the best protocol for platelet glycoprotein (GPIIb/IIIa) analysis using flow cytometry? Fresh platelets should always be used. Storing platelets dramatically changes the level of transmembrane proteins. The best way is to follow one of standardized protocols defined

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