Atomic force microscopy

Transmission of mechanical force is crucial for normal cell development and functioning. However, the process of mechanotransduction cannot be studied in isolation from cell mechanics. Thus, in order to understand how cells ‘feel’, we must first ...

Providing unique insights into life sciences research including physical structure, biochemical interactions, and mechanical properties

Open access peer-reviewed chapter

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Fig.1: Atomic-Force Microscopy Setup

Polar Covalent Bonding and Molecular Dipoles

Observing the structural dynamics of biomolecules is vital to deepening our understanding of biomolecular functions. High-speed (HS) atomic force microscopy (AFM) is a powerful method to measure biomolecular behavior at near physiological conditions. In the AFM, measured image profiles on a molecular surface are distorted by the tip shape through the interactions between the tip and molecule. Once the tip shape is known, AFM images can be approximately deconvolved to reconstruct the surface…