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Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity

Nature volume 312, pages 721–724 (1984)Cite this article

Abstract

A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sarcomas in vivo.

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Authors and Affiliations

  1. Department of Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Patrick W. Gray, Charles V. Benton, Timothy S. Bringman, Julie A. Jarrett, David W. Leung, Peter Ng & Glenn E. Nedwin

  2. Department of Protein Biochemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Bharat B. Aggarwal, William J. Henzel & Barbara Moffat

  3. Department of Pharmacological Sciences, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Lloyd P. Svedersky & Michael A. Palladino

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  1. Patrick W. Gray
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Gray, P., Aggarwal, B., Benton, C. et al. Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity. Nature 312, 721–724 (1984). https://doi.org/10.1038/312721a0

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