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Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin

Nature volume 312, pages 724–729 (1984)Cite this article

Abstract

Human tumour necrosis factor has about 30% homology in its amino acid sequence with lymphotoxin, a lymphokine that has similar biological properties. Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.

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Authors and Affiliations

  1. Department of Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Diane Pennica, Glenn E. Nedwin, Joel S. Hayflick, Peter H. Seeburg, Rik Derynck & David V. Goeddel

  2. Department of Pharmacological Sciences, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Michael A. Palladino

  3. Department of Protein Biochemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    William J. Kohr & Bharat B. Aggarwal

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  1. Diane Pennica
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  3. Joel S. Hayflick
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  9. David V. Goeddel
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Pennica, D., Nedwin, G., Hayflick, J. et al. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature 312, 724–729 (1984). https://doi.org/10.1038/312724a0

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  • DOI: https://doi.org/10.1038/312724a0

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