Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

Sayaka WAKAYAMA; Nguyen Van THUAN; Satoshi KISHIGAMI; Hiroshi OHTA; Eiji MIZUTANI; Takafusa HIKICHI; Masashi MIYAKE; Teruhiko WAKAYAMA

doi:10.1262/jrd.50.627

Original Article

Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

Sayaka WAKAYAMA, Nguyen Van THUAN, Satoshi KISHIGAMI, Hiroshi OHTA, Eiji MIZUTANI, Takafusa HIKICHI, Masashi MIYAKE, Teruhiko WAKAYAMA

Author information

Sayaka WAKAYAMA
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
Department of Life Science, Graduate School of Science and Technology, Kobe University
Nguyen Van THUAN
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
Satoshi KISHIGAMI
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
Hiroshi OHTA
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
Eiji MIZUTANI
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
Graduate School of Agricultural Science, Tohoku University
Takafusa HIKICHI
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe
Masashi MIYAKE
Department of Life Science, Graduate School of Science and Technology, Kobe University
Teruhiko WAKAYAMA
Laboratory for Genomic Reprogramming, Center for Developmental Biology (CDB), Riken Kobe

Keywords: Aging, Preservation, Offspring, Room temperature

JOURNAL FREE ACCESS

2004 Volume 50 Issue 6 Pages 627-637

DOI https://doi.org/10.1262/jrd.50.627

Details

Abstract

To determine the feasibility of preserving oocytes without freezing, we stored mouse oocytes in several media at different temperatures for one day. Confocal microscopy of the metaphase-II spindle in these stored oocytes revealed gross abnormalities in both the spindle and the arrangement of chromosomes. The abnormal spindles could not be rescued by transplanting the aged spindle-chromosome complex into a fresh enucleated oocyte. A diploid parthenogenetic development showed that some of the oocytes stored at room temperature could still develop into blastocysts (10-57%). However, oocytes stored in a refrigerator (5%) or incubator (0%) lost the potential almost entirely. Fertilization of room-temperature-preserved oocytes with fresh spermatozoa by ICSI or IVF resulted in, respectively, 4 and 10%, full-term births. These results suggest that when oocytes are stored at room temperature for one day, most have irreversible damage not only to their cytoplasm but also to the spindle. However, since at least a few percent of stored oocytes retained the potential for full-term development, it may be possible to overcome these problems and develop a simple method for preserving mammalian oocytes without freezing.

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