Modified Vaccinia Virus Ankara Triggers Type I IFN Production in Murine Conventional Dendritic Cells via a cGAS/STING-Mediated Cytosolic DNA-Sensing Pathway
Figure 2
Transcription factors IRF3/IRF7 and the type I IFN positive feedback loop mediated by IFNAR1 are required for the induction of type I IFN in murine cDCs by MVA.
GM-CSF-BMDCs were generated from IRF3−/− (A), IRF7−/− (B), IFNAR1−/− (C) mice, and their age-matched WT controls. Cells (1×106) were stimulated with CpG or infected with MVA at a MOI of 10. Supernatants were collected 22 h later. The concentrations of IFN-α and IFN-β were determined by ELISA. Data are means ± SEM (n = 3). A representative experiment is shown, repeated twice. *, p<0.05; **, p<0.01; ***, p<0.001; comparisons were made between WT cells and various knockout cells as indicated.