REV3L

Protein reversionless 3-like (REV3L) also known as DNA polymerase zeta catalytic subunit (POLZ) is an enzyme that in humans is encoded by the REV3L gene.

REV3L
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3ABD, 3ABE, 3VU7, 4EXT, 4GK0, 4GK5
Identifiers
Aliases	REV3L, POLZ, REV3, REV3 like, DNA directed polymerase zeta catalytic subunit
External IDs	OMIM: 602776; MGI: 1337131; HomoloGene: 48147; GeneCards: REV3L; OMA:REV3L - orthologs
Gene location (Human) Chr. Chromosome 6 (human) Band 6q21 Start 111,299,028 bp End 111,483,715 bp
Gene location (Mouse) Chr. Chromosome 10 (mouse) Band 10|10 B1 Start 39,608,114 bp End 39,751,207 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon skin of hip endometrium body of uterus myometrium synovial joint testicle canal of the cervix tail of epididymis skin of thigh Top expressed in ciliary body pineal gland vestibular sensory epithelium barrel cortex Rostral migratory stream iris retinal pigment epithelium vas deferens conjunctival fornix human fetus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding DNA binding 4 iron, 4 sulfur cluster binding nucleotidyltransferase activity iron-sulfur cluster binding metal ion binding protein binding nucleic acid binding DNA-directed DNA polymerase activity 3'-5' exonuclease activity Cellular component nucleoplasm nucleolus nucleus zeta DNA polymerase complex Biological process DNA-dependent DNA replication error-prone translesion synthesis cellular response to DNA damage stimulus DNA replication DNA repair translesion synthesis nucleic acid phosphodiester bond hydrolysis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5980 19714 Ensembl ENSG00000009413 ENSMUSG00000019841 UniProt O60673 Q61493 RefSeq (mRNA) NM_001286431 NM_001286432 NM_002912 NM_001372078 NM_011264 RefSeq (protein) NP_001273360 NP_001273361 NP_002903 NP_001359007 NP_035394 Location (UCSC) Chr 6: 111.3 – 111.48 Mb Chr 10: 39.61 – 39.75 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

The Rev3 subunit interacts with Rev7 to form Pol ζ, a B family polymerase. Pol ζ lacks 3' to 5' exonuclease activity and is a moderate fidelity polymerase. It cannot add nucleotides across from DNA lesions, yet it can extend from primers with terminal mismatches. This makes Pol ζ very important in translesion synthesis (TLS), because it can act in concert with other TLS polymerases that can add across the lesion to complete the bypass of the lesion. Most polymerases have difficulty extending mismatches because they cannot bind properly to the mismatched DNA. So rather than the cell dying, it can survive albeit with a mutation that may or may not be deleterious, so it is believed that Pol ζ is a driving force of evolution.

Interactions

REV3L has been shown to interact with MAD2L2.

See also

• REV1

Further reading

• Xiao W, Lechler T, Chow BL, et al. (1998). "Identification, chromosomal mapping and tissue-specific expression of hREV3 encoding a putative human DNA polymerase zeta". Carcinogenesis. 19 (5): 945–9. doi:10.1093/carcin/19.5.945. PMID 9635887.
• Lin W, Wu X, Wang Z (1999). "A full-length cDNA of hREV3 is predicted to encode DNA polymerase zeta for damage-induced mutagenesis in humans". Mutat. Res. 433 (2): 89–98. doi:10.1016/s0921-8777(98)00065-2. PMID 10102035.
• Karayianni E, Magnanini C, Orphanos V, et al. (2000). "Transcriptional map of chromosome region 6q16→q21". Cytogenet. Cell Genet. 86 (3–4): 263–6. doi:10.1159/000015356. PMID 10575223. S2CID 39490958.
• Murakumo Y, Roth T, Ishii H, et al. (2000). "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2". J. Biol. Chem. 275 (6): 4391–7. doi:10.1074/jbc.275.6.4391. PMID 10660610.
• Masutani C, Kusumoto R, Iwai S, Hanaoka F (2000). "Mechanisms of accurate translesion synthesis by human DNA polymerase eta". EMBO J. 19 (12): 3100–9. doi:10.1093/emboj/19.12.3100. PMC 203367. PMID 10856253.
• Kawamura K, O-Wang J, Bahar R, et al. (2001). "The error-prone DNA polymerase zeta catalytic subunit (Rev3) gene is ubiquitously expressed in normal and malignant human tissues". Int. J. Oncol. 18 (1): 97–103. doi:10.3892/ijo.18.1.97. PMID 11115544.
• Murakumo Y, Ogura Y, Ishii H, et al. (2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. 276 (38): 35644–51. doi:10.1074/jbc.M102051200. PMID 11485998.
• Li Z, Zhang H, McManus TP, et al. (2003). "hREV3 is essential for error-prone translesion synthesis past UV or benzo[a]pyrene diol epoxide-induced DNA lesions in human fibroblasts". Mutat. Res. 510 (1–2): 71–80. doi:10.1016/S0027-5107(02)00253-1. PMID 12459444.
• Zhu F, Jin CX, Song T, et al. (2003). "Response of human REV3 gene to gastric cancer inducing carcinogen N-methyl-N'-nitro-N-nitrosoguanidine and its role in mutagenesis". World J. Gastroenterol. 9 (5): 888–93. doi:10.3748/wjg.v9.i5.888. PMC 4611392. PMID 12717825.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. doi:10.1038/nature02055. PMID 14574404.
• Ohashi E, Murakumo Y, Kanjo N, et al. (2005). "Interaction of hREV1 with three human Y-family DNA polymerases". Genes Cells. 9 (6): 523–31. doi:10.1111/j.1356-9597.2004.00747.x. PMID 15189446. S2CID 24470762.
• Tao WA, Wollscheid B, O'Brien R, et al. (2005). "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nat. Methods. 2 (8): 591–8. doi:10.1038/nmeth776. PMID 16094384. S2CID 20475874.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.